################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AluI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstUI BstXI Bsu36I CelII CfoI Cfr10I Cfr13I CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MluI MluNI MroI MscI MspA1I MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 257 GTATAC GT^[AC][GT]AC AciI 162 GCGG CCGC(-3/-1) AcsI 26 AAATTT [GA]^AATT[CT] AfaI 215 GTAC GT^AC Alw44I 347 GTGCAC G^TGCAC AlwI 424 GATCC GGATC(4/5) ApaLI 347 GTGCAC G^TGCAC ApoI 26 AAATTT [GA]^AATT[CT] AspHI 347 GTGCAC G[AT]GC[AT]^C BanI 312 GGTGCC G^G[CT][GA]CC BbvI 263 GCTGC GCAGC(8/12) BcgI 181 CGATGGCGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 309 CCGGG CC^[GC]GG BsiHKAI 347 GTGCAC G[AT]GC[AT]^C BspDI 179 ATCGAT AT^CGAT BspMI 368 GCAGGT ACCTGC(4/8) BsrI 457 CCAGT ACTGG(1/-1) Bst1107I 257 GTATAC GTA^TAC BstEII 247 GGTAACC G^GT[ACGT]ACC BstF5I 117 CATCC GGATG(2/0) BstPI 247 GGTAACC G^GT[ACGT]ACC BstYI 423 AGATCC [GA]^GATC[CT] BstZ17I 257 GTATAC GTA^TAC Cac8I 162 GCGGGC GC[ACGT]^[ACGT]GC ClaI 179 ATCGAT AT^CGAT DdeI 104 CTCAG C^T[ACGT]AG DpnII 424 GATC ^GATC EcoO65I 247 GGTAACC G^GT[ACGT]ACC FauI 162 GCGGG CCCGC(4/6) Fnu4HI 263 GCTGC GC^[ACGT]GC FokI 117 CATCC GGATG(9/13) HapII 309 CCGG C^CGG HpaII 309 CCGG C^CGG HphI 462 TCACC GGTGA(8/7) ItaI 263 GCTGC GC^[ACGT]GC MboI 424 GATC ^GATC MfeI 288 CAATTG C^AATTG MflI 423 AGATCC [GA]^GATC[CT] MslI 153 CATTGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 309 CCGG C^CGG MunI 288 CAATTG C^AATTG MwoI 157 GCAATGCGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 309 CCGGG CC^[GC]GG NdeII 424 GATC ^GATC NspI 409 GCATGT [GA]CATG^[CT] PstI 366 CTGCAG CTGCA^G RsaI 215 GTAC GT^AC Sau3AI 424 GATC ^GATC SfaNI 146 GATGC GCATC(5/9) SfcI 366 CTGCAG C^T[GA][CT]AG SspI 355 AATATT AAT^ATT TseI 263 GCTGC G^C[AT]GC XhoII 423 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BanII 66 GGGCTC G[GA]GC[CT]^C 101 GGGCTC BciVI 468 GGATAC GTATCC(6/5) 444 GTATCC BsaJI 427 CCACGG C^C[ACGT][ACGT]GG 439 CCACGG BsrDI 153 CATTGC GCAATG(2/0) 157 GCAATG DsaI 427 CCACGG C^C[GA][CT]GG 439 CCACGG HinfI 305 GATTC G^A[ACGT]TC 433 GATTC MboII 39 TCTTC GAAGA(8/7) 210 TCTTC MseI 170 TTAA T^TAA 236 TTAA NlaIII 76 CATG CATG^ 410 CATG TaqI 144 TCGA T^CGA 180 TCGA TfiI 305 GATTC G^A[AT]TC 433 GATTC Tru9I 170 TTAA T^TAA 236 TTAA Tsp45I 329 GTGAC ^GT[GC]AC 413 GTGAC TspRI 106 CAGTG CA[GC]TG[ACGT][ACGT]^ 325 CACTG TthHB8I 144 TCGA T^CGA 180 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### BmyI Bsp1286I BssKI BstNI EcoRII MaeIII MnlI MvaI NlaIV ScrFI Tsp509I