Restriction map for
pFN21AE5167
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# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AflIII AgeI AhdI AlwI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstF5I BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DpnII DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FokI FseI
FspI HgaI HincII HindII HindIII
HpaI Ksp632I KspI MaeI MaeII
MamI MboI MboII MfeI MflI
MluI MluNI MroI MscI MseI
MunI MvnI NaeI NcoI NdeII
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tru9I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 356 GGTACC G^GTACC
AcsI 103 AAATTC [GA]^AATT[CT]
AcyI 176 GGCGCC G[GA]^CG[CT]C
AfaI 357 GTAC GT^AC
AluI 148 AGCT AG^CT
Alw44I 223 GTGCAC G^TGCAC
ApaLI 223 GTGCAC G^TGCAC
ApoI 103 AAATTC [GA]^AATT[CT]
Asp718I 356 GGTACC G^GTACC
AspHI 223 GTGCAC G[AT]GC[AT]^C
AvaI 92 CCCGGG C^[CT]CG[GA]G
BbeI 176 GGCGCC GGCGC^C
BsaHI 176 GGCGCC G[GA]^CG[CT]C
BsaWI 374 ACCGGA [AT]^CCGG[AT]
BsiHKAI 223 GTGCAC G[AT]GC[AT]^C
BsoBI 92 CCCGGG C^[CT]CG[GA]G
BspMI 274 ACCTGC ACCTGC(4/8)
BssSI 226 CACGAG CACGAG(-5/-1)
BstEII 270 GGTCACC G^GT[ACGT]ACC
BstPI 270 GGTCACC G^GT[ACGT]ACC
Cac8I 317 GCATGC GC[ACGT]^[ACGT]GC
CfoI 177 GCGC GCG^C
DdeI 126 CTCAG C^T[ACGT]AG
EcoNI 78 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 270 GGTCACC G^GT[ACGT]ACC
HaeII 176 GGCGCC [GA]GCGC^[CT]
HhaI 177 GCGC GCG^C
Hin1I 176 GGCGCC G[GA]^CG[CT]C
HinP1I 177 GCGC G^CGC
HphI 272 TCACC GGTGA(8/7)
KasI 176 GGCGCC G^GCGCC
KpnI 356 GGTACC GGTAC^C
MspA1I 109 CAGCGG C[AC]G^C[GT]G
NarI 176 GGCGCC GG^CGCC
NlaIII 318 CATG CATG^
NspI 317 GCATGC [GA]CATG^[CT]
RsaI 357 GTAC GT^AC
SfaNI 445 GATGC GCATC(5/9)
SfoI 176 GGCGCC GGC^GCC
SmaI 92 CCCGGG CCC^GGG
SphI 317 GCATGC GCATG^C
TfiI 88 GAATC G^A[AT]TC
Tsp45I 271 GTCAC ^GT[GC]AC
Tsp509I 104 AATT ^AATT
TspRI 209 CAGTG CA[GC]TG[ACGT][ACGT]^
XmaCI 92 CCCGGG C^CCGGG
XmaI 92 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AvaII 66 GGTCC G^G[AT]CC
377 GGACC
BanI 176 GGCGCC G^G[CT][GA]CC
356 GGTACC
BanII 229 GAGCCC G[GA]GC[CT]^C
420 GAGCCC
BcnI 92 CCCGG CC^[GC]GG
93 CCGGG
BsmAI 393 GAGAC GTCTC(1/5)
15 GTCTC
BsrI 208 CCAGT ACTGG(1/-1)
433 CCAGT
HaeIII 96 GGCC GG^CC
408 GGCC
MaeIII 161 GTAAC ^GT[ACGT]AC
271 GTCAC
MnlI 174 GAGG CCTC(7/6)
457 GAGG
NciI 92 CCCGG CC^[GC]GG
93 CCGGG
NdeI 118 CATATG CA^TATG
425 CATATG
PleI 124 GACTC GAGTC(4/5)
460 GACTC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI BbvI BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BssKI BstNI
Cfr13I EcoRII Fnu4HI HapII HinfI
HpaII ItaI MslI MspI MvaI
MwoI NlaIV Sau96I ScrFI TseI