(Circular) (Six-base) MAPSORT of: pFN21AE5167.seq Check: 4338 from: 1 to: 5173 pFN21AE5167 5173 bp With 182 enzymes: SgfI * January 30, 2014 17:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2288 2288 Size: 5173 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2983 278 Size: 53 83 186 936 1447 2468 Fragments arranged by size: 2468 1447 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2516 1937 Size: 579 4594 Acc65I G'GTAC_C Cuts at: 2361 2494 2361 Size: 133 5040 AclI AA'CG_TT Cuts at: 1563 3300 3673 1563 Size: 1737 373 3063 Fragments arranged by size: 3063 1737 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3236 4620 4888 1632 Size: 1604 1384 268 1917 Fragments arranged by size: 1917 1604 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4976 AflIII A'CryG_T Cuts at: 1203 4072 5049 1203 Size: 2869 977 1327 Fragments arranged by size: 2869 1327 977 AgeI A'CCGG_T Cuts at: 4004 4004 Size: 5173 AhdI GACnn_n'nnGTC Cuts at: 3902 3902 Size: 5173 AleI CACnn'nnGTG Cuts at: 1166 2225 1166 Size: 1059 4114 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5173 AlwNI CAG_nnn'CTG Cuts at: 2916 4488 2916 Size: 1572 3601 ApaLI G'TGCA_C Cuts at: 2228 3230 4386 2228 Size: 1002 1156 3015 Fragments arranged by size: 3015 1156 1002 ApoI r'AATT_y Cuts at: 1493 2108 2482 2708 2860 4969 1493 Size: 615 374 226 152 2109 1697 Fragments arranged by size: 2109 1697 615 374 226 152 AseI AT'TA_AT Cuts at: 160 1032 3727 160 Size: 872 2695 1606 Fragments arranged by size: 2695 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5173 AvaI C'yCGr_G Cuts at: 1870 1943 2097 2498 1870 Size: 73 154 401 4545 Fragments arranged by size: 4545 401 154 73 BamHI G'GATC_C Cuts at: 2503 2870 2503 Size: 367 4806 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2181 2361 2494 3949 618 Size: 325 217 627 394 180 133 1455 1842 Fragments arranged by size: 1842 1455 627 394 325 217 180 133 BanII G_rGCy'C Cuts at: 1391 2238 2429 2492 1391 Size: 847 191 63 4072 Fragments arranged by size: 4072 847 191 63 BbeI G_GCGC'C Cuts at: 2185 2185 Size: 5173 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4202 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3364 3398 1903 Size: 34 1427 34 3678 Fragments arranged by size: 3678 1427 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3065 4275 3065 Size: 1210 3963 BclI T'GATC_A Cuts at: 1548 1548 Size: 5173 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3784 136 Size: 107 122 71 631 605 2112 1525 Fragments arranged by size: 2112 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5168 5168 Size: 5173 BlpI GC'TnA_GC Cuts at: 2590 2590 Size: 5173 Bme1580I G_kGCm'C Cuts at: 1790 2232 3234 4390 1790 Size: 442 1002 1156 2573 Fragments arranged by size: 2573 1156 1002 442 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5173 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2904 3862 442 Size: 416 53 83 399 24 1487 958 1753 Fragments arranged by size: 1753 1487 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5173 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2033 2302 2341 3833 1239 Size: 794 269 39 1492 2579 Fragments arranged by size: 2579 1492 794 269 39 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3298 4163 4461 3298 Size: 865 298 4010 Fragments arranged by size: 4010 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3836 882 Size: 617 2337 2219 Fragments arranged by size: 2337 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2964 493 Size: 711 1760 2702 Fragments arranged by size: 2702 1760 711 BsaBI GATnn'nnATC Cuts at: 5167 5167 Size: 5173 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2182 2980 3362 3997 Size: 53 83 186 936 649 798 382 635 Cuts at: 3997 275 Size: 1451 Fragments arranged by size: 1451 936 798 649 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2379 3606 4004 4278 4425 4836 2379 Size: 1227 398 274 147 411 2716 Fragments arranged by size: 2716 1227 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4948 Fragments arranged by size: 4948 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5173 BseYI C'CCAG_C Cuts at: 2062 2067 2144 4376 4872 2062 Size: 5 77 2232 496 2363 Fragments arranged by size: 2363 2232 496 77 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4600 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2623 3384 4412 1540 Size: 275 186 622 761 1028 2301 Fragments arranged by size: 2301 1028 761 622 275 186 BsiHKAI G_wGCw'C Cuts at: 2232 3234 3319 4390 2232 Size: 1002 85 1071 3015 Fragments arranged by size: 3015 1071 1002 85 BsmI GAATG_Cn' Cuts at: 2684 2777 2684 Size: 93 5080 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2232 2238 2429 2492 3234 3319 4390 Size: 399 442 6 191 63 742 85 1071 Cuts at: 4390 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 442 399 191 85 63 6 BspEI T'CCGG_A Cuts at: 4836 4836 Size: 5173 BspHI T'CATG_A Cuts at: 3062 4792 3062 Size: 1730 3443 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2288 2528 844 Size: 1444 240 3489 Fragments arranged by size: 3489 1444 240 BsrBI CCG'CTC Cuts at: 1394 1823 3060 1394 Size: 429 1237 3507 Fragments arranged by size: 3507 1237 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3668 3842 65 Size: 1183 2420 174 1396 Fragments arranged by size: 2420 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3817 4004 1700 Size: 110 2007 187 2869 Fragments arranged by size: 2869 2007 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5173 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5173 BssSI C'ACGA_G Cuts at: 2231 3233 4245 2231 Size: 1002 1012 3159 Fragments arranged by size: 3159 1012 1002 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5173 BstBI TT'CG_AA Cuts at: 3977 3977 Size: 5173 BstEII G'GTnAC_C Cuts at: 1184 2275 1184 Size: 1091 4082 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5173 BstYI r'GATC_y Cuts at: 1972 2503 2870 3255 3272 4724 4810 4822 5168 Size: 531 367 385 17 1452 86 12 346 Cuts at: 5168 1972 Size: 1977 Fragments arranged by size: 1977 1452 531 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3682 Fragments arranged by size: 3682 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4434 Fragments arranged by size: 4434 683 56 BtsI GCAGTG_nn' Cuts at: 2689 3483 3503 2689 Size: 794 20 4359 Fragments arranged by size: 4359 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5173 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5138 DraI TTT'AAA Cuts at: 2477 2477 Size: 5173 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4180 809 Size: 507 2864 1802 Fragments arranged by size: 2864 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2620 3509 5138 Size: 54 1425 50 275 18 790 889 1629 Cuts at: 5138 8 Size: 43 Fragments arranged by size: 1629 1425 889 790 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2620 1537 Size: 275 808 4090 Fragments arranged by size: 4090 808 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3103 1353 Size: 1750 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3760 4134 4280 387 Size: 1064 138 2171 374 146 1280 Fragments arranged by size: 2171 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2302 2341 3236 3833 4620 4888 Size: 393 401 269 39 895 597 787 268 Cuts at: 4888 1239 Size: 1524 Fragments arranged by size: 1524 895 787 597 401 393 269 268 39 EcoNI CCTnn'n_nnAGG Cuts at: 2087 2087 Size: 5173 EcoRI G'AATT_C Cuts at: 2482 2860 2482 Size: 378 4795 FspI TGC'GCA Cuts at: 3679 3679 Size: 5173 HaeII r_GCGC'y Cuts at: 1678 2185 4320 1678 Size: 507 2135 2531 Fragments arranged by size: 2531 2135 507 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3814 3846 3888 3920 1373 Size: 32 135 32 2242 32 42 32 2626 Fragments arranged by size: 2626 2242 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2517 2763 669 Size: 988 281 579 246 3079 Fragments arranged by size: 3079 988 579 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5173 HpaI GTT'AAC Cuts at: 2763 2763 Size: 5173 KasI G'GCGC_C Cuts at: 2181 2181 Size: 5173 KpnI G_GTAC'C Cuts at: 2365 2498 2365 Size: 133 5040 MfeI C'AATT_G Cuts at: 2772 2772 Size: 5173 MluI A'CGCG_T Cuts at: 5049 5049 Size: 5173 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4287 4471 1305 Size: 2982 184 2007 Fragments arranged by size: 2982 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3351 Fragments arranged by size: 3351 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2225 2408 2448 3131 3490 3649 518 Size: 648 1059 183 40 683 359 159 2042 Fragments arranged by size: 2042 1059 683 648 359 183 159 40 MspA1I CmG'CkG Cuts at: 1606 2116 2589 3266 4414 4659 1606 Size: 510 473 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 510 473 245 NarI GG'CG_CC Cuts at: 2182 2182 Size: 5173 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3682 Fragments arranged by size: 3682 939 552 NdeI CA'TA_TG Cuts at: 387 2124 2431 387 Size: 1737 307 3129 Fragments arranged by size: 3129 1737 307 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5173 NotI GC'GGCC_GC Cuts at: 2620 2620 Size: 5173 NspI r_CATG'y Cuts at: 1132 1229 2326 2531 4076 1132 Size: 97 1097 205 1545 2229 Fragments arranged by size: 2229 1545 1097 205 97 PasI CC'CwG_GG Cuts at: 1348 2083 1348 Size: 735 4438 PciI A'CATG_T Cuts at: 4072 4072 Size: 5173 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5173 PfoI T'CCnGG_A Cuts at: 2265 2265 Size: 5173 PmeI GTTT'AAAC Cuts at: 2477 2477 Size: 5173 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3242 3274 4755 4787 3242 Size: 32 1481 32 3628 Fragments arranged by size: 3628 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5173 PsiI TTA'TAA Cuts at: 2743 2743 Size: 5173 PstI C_TGCA'G Cuts at: 830 2525 830 Size: 1695 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5173 SalI G'TCGA_C Cuts at: 1936 2515 1936 Size: 579 4594 SbfI CC_TGCA'GG Cuts at: 2525 2525 Size: 5173 ScaI AGT'ACT Cuts at: 3421 5133 3421 Size: 1712 3461 SfcI C'TryA_G Cuts at: 826 1045 2521 3656 4337 4528 826 Size: 219 1476 1135 681 191 1471 Fragments arranged by size: 1476 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2183 2183 Size: 5173 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5173 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5173 SmaI CCC'GGG Cuts at: 1872 2099 2500 1872 Size: 227 401 4545 Fragments arranged by size: 4545 401 227 SmlI C'TyrA_G Cuts at: 820 1017 1943 3277 4178 4440 820 Size: 197 926 1334 901 262 1553 Fragments arranged by size: 1553 1334 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2964 493 Size: 2471 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5173 SphI G_CATG'C Cuts at: 1132 2326 2531 1132 Size: 1194 205 3774 Fragments arranged by size: 3774 1194 205 SspI AAT'ATT Cuts at: 5 52 3097 5 Size: 47 3045 2081 Fragments arranged by size: 3045 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5173 StyI C'CwwG_G Cuts at: 513 1065 2004 2612 513 Size: 552 939 608 3074 Fragments arranged by size: 3074 939 608 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3200 3217 3370 3555 4000 325 Size: 1255 1620 17 153 185 445 1498 Fragments arranged by size: 1620 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3419 5131 96 Size: 274 80 33 51 1443 1442 1712 138 Fragments arranged by size: 1712 1443 1442 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5133 5165 5133 Size: 32 5141 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5173 XbaI T'CTAG_A Cuts at: 2509 2509 Size: 5173 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5173 XmaI C'CCGG_G Cuts at: 1870 2097 2498 1870 Size: 227 401 4545 Fragments arranged by size: 4545 401 227 XmnI GAAnn'nnTTC Cuts at: 3302 3302 Size: 5173 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2981 276 Size: 53 83 186 936 1447 2468 Fragments arranged by size: 2468 1447 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI