Restriction map for
pFN21AB0431
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
Acc65I AccI AccIII AfaI AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BciVI
BclI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HaeII HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PvuI
RcaI RsaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 1005 GACGTC GACGT^C
AclI 740 AACGTT AA^CGTT
AcsI 689 GAATTC [GA]^AATT[CT]
AflII 850 CTTAAG C^TTAAG
ApaI 54 GGGCCC GGGCC^C
ApoI 689 GAATTC [GA]^AATT[CT]
AseI 346 ATTAAT AT^TAAT
AsnI 346 ATTAAT AT^TAAT
AvaI 57 CCCGAG C^[CT]CG[GA]G
BalI 1176 TGGCCA TGG^CCA
BfrI 850 CTTAAG C^TTAAG
BglI 1122 GCCTGAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1048 AGATCT A^GATCT
BsaWI 148 TCCGGT [AT]^CCGG[AT]
BsiEI 38 CGGCCG CG[GA][CT]^CG
BsmFI 1396 GGGAC GGGAC(10/14)
BsoBI 57 CCCGAG C^[CT]CG[GA]G
BsrBI 60 GAGCGG CCGCTC(-3/-3)
BsrDI 627 CATTGC GCAATG(2/0)
BsrFI 1304 ACCGGC [GA]^CCGG[CT]
BssSI 1117 CACGAG CACGAG(-5/-1)
BstXI 968 CCAGGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1304 ACCGGC [GA]^CCGG[CT]
EagI 38 CGGCCG C^GGCCG
EclXI 38 CGGCCG C^GGCCG
Eco52I 38 CGGCCG C^GGCCG
EcoRI 689 GAATTC G^AATTC
HgaI 129 GACGC GACGC(5/10)
HindIII 844 AAGCTT A^AGCTT
MluNI 1176 TGGCCA TGG^CCA
MscI 1176 TGGCCA TGG^CCA
NruI 177 TCGCGA TCG^CGA
PpuMI 1170 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 346 ATTAAT AT^TAAT
Psp1406I 740 AACGTT AA^CGTT
PstI 434 CTGCAG CTGCA^G
SapI 1244 GAAGAGC GCTCTTC(1/4)
SfcI 434 CTGCAG C^T[GA][CT]AG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
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AcyI 129 GACGCC G[GA]^CG[CT]C
1005 GACGTC
BbsI 567 GAAGAC GAAGAC(2/6)
612 GAAGAC
BcgI 684 GCAGAGAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1384 GCAGAAGGTTCG
BpuAI 567 GAAGAC GAAGAC(2/6)
612 GAAGAC
BsaHI 129 GACGCC G[GA]^CG[CT]C
1005 GACGTC
BsgI 91 GTGCAG GTGCAG(16/14)
953 GTGCAG
BsmBI 127 GAGACG CGTCTC(1/5)
1003 GAGACG
BstYI 1048 AGATCT [GA]^GATC[CT]
1373 GGATCT
DraII 53 GGGGCCC [GA]G^G[ACGT]CC[CT]
1170 AGGACCT
DsaI 974 CCATGG C^C[GA][CT]GG
1229 CCATGG
EcoNI 198 CCTGAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
234 CCTCACCAAGG
EcoO109I 53 GGGGCCC [GA]G^G[ACGT]CC[CT]
1170 AGGACCT
Hin1I 129 GACGCC G[GA]^CG[CT]C
1005 GACGTC
HphI 236 TCACC GGTGA(8/7)
1113 TCACC
MaeII 741 ACGT A^CGT
1006 ACGT
MaeIII 1008 GTCAC ^GT[ACGT]AC
1259 GTGAC
MflI 1048 AGATCT [GA]^GATC[CT]
1373 GGATCT
MslI 1033 CACAGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1084 CATGTGGATG
NcoI 974 CCATGG C^CATGG
1229 CCATGG
PshAI 307 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1261 GACGACAGTC
StuI 27 AGGCCT AGG^CCT
420 AGGCCT
Tsp45I 1008 GTCAC ^GT[GC]AC
1259 GTGAC
XhoII 1048 AGATCT [GA]^GATC[CT]
1373 GGATCT
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI Ksp632I MaeI
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
SacI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XcmI