(Circular) (Six-base) MAPSORT of: pFN21AB0431.seq Check: 5328 from: 1 to: 6133 pFN21AB0431 6133 bp With 182 enzymes: SgfI * January 27, 2011 14:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3014 3943 278 Size: 53 83 186 936 1478 929 2468 Fragments arranged by size: 2468 1478 936 929 186 83 53 AccI GT'mk_AC Cuts at: 1937 3476 1937 Size: 1539 4594 Acc65I G'GTAC_C Cuts at: 3454 3454 Size: 6133 AclI AA'CG_TT Cuts at: 1563 2746 4260 4633 1563 Size: 1183 1514 373 3063 Fragments arranged by size: 3063 1514 1183 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3093 4196 5580 5848 1632 Size: 1461 1103 1384 268 1917 Fragments arranged by size: 1917 1461 1384 1103 268 AflII C'TTAA_G Cuts at: 820 1017 2855 820 Size: 197 1838 4098 Fragments arranged by size: 4098 1838 197 AflIII A'CryG_T Cuts at: 1203 5032 6009 1203 Size: 3829 977 1327 Fragments arranged by size: 3829 1327 977 AgeI A'CCGG_T Cuts at: 4964 4964 Size: 6133 AhdI GACnn_n'nnGTC Cuts at: 4862 4862 Size: 6133 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6133 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6133 AlwNI CAG_nnn'CTG Cuts at: 3876 5448 3876 Size: 1572 4561 ApaI G_GGCC'C Cuts at: 2063 2063 Size: 6133 ApaLI G'TGCA_C Cuts at: 4190 5346 4190 Size: 1156 4977 ApoI r'AATT_y Cuts at: 1493 2694 3442 3668 3820 5929 1493 Size: 1201 748 226 152 2109 1697 Fragments arranged by size: 2109 1697 1201 748 226 152 AseI AT'TA_AT Cuts at: 160 1032 2352 4687 160 Size: 872 1320 2335 1606 Fragments arranged by size: 2335 1606 1320 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 AvaI C'yCGr_G Cuts at: 1870 1943 2062 3458 1870 Size: 73 119 1396 4545 Fragments arranged by size: 4545 1396 119 73 BamHI G'GATC_C Cuts at: 3463 3830 3463 Size: 367 5766 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3454 4909 618 Size: 325 217 627 1667 1455 1842 Fragments arranged by size: 1842 1667 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2063 2631 3003 3118 3223 3343 3452 1391 Size: 672 568 372 115 105 120 109 4072 Fragments arranged by size: 4072 672 568 372 120 115 109 105 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2579 2624 928 Size: 971 680 45 4437 Fragments arranged by size: 4437 971 680 45 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2678 2712 3378 3412 4324 Size: 34 50 34 657 34 666 34 912 Cuts at: 4324 4358 1903 Size: 34 3678 Fragments arranged by size: 3678 912 666 657 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4025 5235 4025 Size: 1210 4923 BclI T'GATC_A Cuts at: 1548 1548 Size: 6133 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3133 4744 136 Size: 107 122 71 631 605 1461 1611 1525 Fragments arranged by size: 1611 1525 1461 631 605 122 107 71 BglII A'GATC_T Cuts at: 3053 6128 3053 Size: 3075 3058 BlpI GC'TnA_GC Cuts at: 3550 3550 Size: 6133 Bme1580I G_kGCm'C Cuts at: 1790 2063 2953 4194 5350 1790 Size: 273 890 1241 1156 2573 Fragments arranged by size: 2573 1241 1156 890 273 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6133 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3254 3864 4822 Size: 416 53 83 399 24 1837 610 958 Cuts at: 4822 442 Size: 1753 Fragments arranged by size: 1837 1753 958 610 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6133 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2276 2946 3014 4793 1239 Size: 1037 670 68 1779 2579 Fragments arranged by size: 2579 1779 1037 670 68 Bpu10I CC'TnA_GC Cuts at: 2204 2204 Size: 6133 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2323 3042 4258 5123 5421 2323 Size: 719 1216 865 298 3035 Fragments arranged by size: 3035 1216 865 719 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4796 882 Size: 617 3297 2219 Fragments arranged by size: 3297 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3924 493 Size: 711 2720 2702 Fragments arranged by size: 2720 2702 711 BsaBI GATnn'nnATC Cuts at: 6127 6127 Size: 6133 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2135 3011 3940 4322 Size: 53 83 186 936 602 876 929 382 Cuts at: 4322 4957 275 Size: 635 1451 Fragments arranged by size: 1451 936 929 876 635 602 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2153 4566 4964 5238 5385 5796 2153 Size: 2413 398 274 147 411 2490 Fragments arranged by size: 2490 2413 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2066 2096 1178 Size: 30 165 30 663 30 5215 Fragments arranged by size: 5215 663 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2201 2273 2737 2740 2922 2927 3011 1186 Size: 1015 72 464 3 182 5 84 4308 Fragments arranged by size: 4308 1015 464 182 84 72 5 3 BseYI C'CCAG_C Cuts at: 2599 5336 5832 2599 Size: 2737 496 2900 Fragments arranged by size: 2900 2737 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2117 2979 1163 Size: 573 381 862 4317 Fragments arranged by size: 4317 862 573 381 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2046 3583 4344 5372 1540 Size: 275 186 45 1537 761 1028 2301 Fragments arranged by size: 2301 1537 1028 761 275 186 45 BsiHKAI G_wGCw'C Cuts at: 2631 3039 3118 3343 4194 4279 5350 2631 Size: 408 79 225 851 85 1071 3414 Fragments arranged by size: 3414 1071 851 408 225 85 79 BsmI GAATG_Cn' Cuts at: 3644 3737 3644 Size: 93 6040 BsmBI CGTCTCn'nnnn_ Cuts at: 2126 3002 2126 Size: 876 5257 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2631 2953 3003 3039 3118 3223 Size: 399 273 568 322 50 36 79 105 Cuts at: 3223 3343 3452 4194 4279 5350 1391 Size: 120 109 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 568 399 322 273 120 109 105 85 79 50 36 BspEI T'CCGG_A Cuts at: 5796 5796 Size: 6133 BspHI T'CATG_A Cuts at: 4022 5752 4022 Size: 1730 4403 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3488 844 Size: 2644 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2067 4020 1394 Size: 429 244 1953 3507 Fragments arranged by size: 3507 1953 429 244 BsrDI GCAATG_nn' Cuts at: 65 1248 2631 4628 4802 65 Size: 1183 1383 1997 174 1396 Fragments arranged by size: 1997 1396 1383 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3309 4777 4964 1700 Size: 110 1499 1468 187 2869 Fragments arranged by size: 2869 1499 1468 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6133 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6133 BssSI C'ACGA_G Cuts at: 3122 4193 5205 3122 Size: 1071 1012 4050 Fragments arranged by size: 4050 1071 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6133 BstBI TT'CG_AA Cuts at: 4937 4937 Size: 6133 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6133 BstXI CCAn_nnnn'nTGG Cuts at: 2980 2980 Size: 6133 BstYI r'GATC_y Cuts at: 1972 3053 3378 3463 3830 4215 4232 5684 5770 Size: 1081 325 85 367 385 17 1452 86 Cuts at: 5770 5782 6128 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1081 385 367 346 325 86 85 17 12 BtgI C'CryG_G Cuts at: 513 1065 2979 3234 513 Size: 552 1914 255 3412 Fragments arranged by size: 3412 1914 552 255 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5394 Fragments arranged by size: 5394 683 56 BtsI GCAGTG_nn' Cuts at: 2448 3031 3649 4443 4463 2448 Size: 583 618 794 20 4118 Fragments arranged by size: 4118 794 618 583 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6133 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6098 DraI TTT'AAA Cuts at: 3437 3437 Size: 6133 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5140 809 Size: 507 3824 1802 Fragments arranged by size: 3824 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 3181 3311 Size: 54 1425 50 275 18 213 1138 130 Cuts at: 3311 3580 4469 6098 8 Size: 269 889 1629 43 Fragments arranged by size: 1629 1425 1138 889 275 269 213 130 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2043 3580 1537 Size: 275 231 1537 4090 Fragments arranged by size: 4090 1537 275 231 EarI CTCTTCn'nnn_ Cuts at: 1353 2186 2562 2697 2751 2770 3244 3410 3417 Size: 833 376 135 54 19 474 166 7 Cuts at: 3417 4063 1353 Size: 646 3423 Fragments arranged by size: 3423 833 646 474 376 166 135 54 19 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2210 3381 4720 5094 5240 387 Size: 1064 138 621 1171 1339 374 146 1280 Fragments arranged by size: 1339 1280 1171 1064 621 374 146 138 EcoICRI GAG'CTC Cuts at: 2629 3116 3341 2629 Size: 487 225 5421 Fragments arranged by size: 5421 487 225 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2276 2946 3014 3093 4196 4793 5580 Size: 393 644 670 68 79 1103 597 787 Cuts at: 5580 5848 1239 Size: 268 1524 Fragments arranged by size: 1524 1103 787 670 644 597 393 268 79 68 EcoNI CCTnn'n_nnAGG Cuts at: 2207 2243 2207 Size: 36 6097 EcoO109I rG'GnC_Cy Cuts at: 2059 3176 2059 Size: 1117 5016 EcoRI G'AATT_C Cuts at: 2694 3442 3820 2694 Size: 748 378 5007 Fragments arranged by size: 5007 748 378 FspI TGC'GCA Cuts at: 4639 4639 Size: 6133 HaeII r_GCGC'y Cuts at: 1678 5280 1678 Size: 3602 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2066 2098 2308 2340 4774 Size: 32 135 32 494 32 210 32 2434 Cuts at: 4774 4806 4848 4880 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2434 494 210 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3477 3723 669 Size: 988 281 1539 246 3079 Fragments arranged by size: 3079 1539 988 281 246 HindIII A'AGCT_T Cuts at: 748 2849 748 Size: 2101 4032 HpaI GTT'AAC Cuts at: 3723 3723 Size: 6133 KpnI G_GTAC'C Cuts at: 3458 3458 Size: 6133 MfeI C'AATT_G Cuts at: 3732 3732 Size: 6133 MluI A'CGCG_T Cuts at: 6009 6009 Size: 6133 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5247 5431 1305 Size: 3942 184 2007 Fragments arranged by size: 3942 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3183 10 Size: 54 1425 343 1351 2960 Fragments arranged by size: 2960 1425 1351 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3042 3093 4091 4450 4609 518 Size: 648 1876 51 998 359 159 2042 Fragments arranged by size: 2042 1876 998 648 359 159 51 MspA1I CmG'CkG Cuts at: 1606 2254 2266 2275 3021 3216 3325 3549 4226 Size: 648 12 9 746 195 109 224 677 Cuts at: 4226 5374 5619 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 746 677 648 245 224 195 109 12 9 NcoI C'CATG_G Cuts at: 513 1065 2979 3234 513 Size: 552 1914 255 3412 Fragments arranged by size: 3412 1914 552 255 NdeI CA'TA_TG Cuts at: 387 387 Size: 6133 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6133 NotI GC'GGCC_GC Cuts at: 3580 3580 Size: 6133 NruI TCG'CGA Cuts at: 2184 2184 Size: 6133 NspI r_CATG'y Cuts at: 1132 1229 3491 5036 1132 Size: 97 2262 1545 2229 Fragments arranged by size: 2262 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6133 PciI A'CATG_T Cuts at: 5032 5032 Size: 6133 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6133 PfoI T'CCnGG_A Cuts at: 3334 3334 Size: 6133 PmeI GTTT'AAAC Cuts at: 3437 3437 Size: 6133 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2635 2667 4202 4234 5715 5747 2635 Size: 32 1535 32 1481 32 3021 Fragments arranged by size: 3021 1535 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3176 3176 Size: 6133 PshAI GACnn'nnGTC Cuts at: 1613 2316 3270 1613 Size: 703 954 4476 Fragments arranged by size: 4476 954 703 PsiI TTA'TAA Cuts at: 3703 3703 Size: 6133 PspOMI G'GGCC_C Cuts at: 2059 2059 Size: 6133 PstI C_TGCA'G Cuts at: 830 2443 3485 830 Size: 1613 1042 3478 Fragments arranged by size: 3478 1613 1042 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6133 PvuII CAG'CTG Cuts at: 2254 2266 3325 2254 Size: 12 1059 5062 Fragments arranged by size: 5062 1059 12 SacI G_AGCT'C Cuts at: 2631 3118 3343 2631 Size: 487 225 5421 Fragments arranged by size: 5421 487 225 SalI G'TCGA_C Cuts at: 1936 3475 1936 Size: 1539 4594 SapI GCTCTTCn'nnn_ Cuts at: 3244 3244 Size: 6133 SbfI CC_TGCA'GG Cuts at: 3485 3485 Size: 6133 ScaI AGT'ACT Cuts at: 4381 6093 4381 Size: 1712 4421 SfcI C'TryA_G Cuts at: 826 1045 2439 3481 4616 5297 5488 826 Size: 219 1394 1042 1135 681 191 1471 Fragments arranged by size: 1471 1394 1135 1042 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6133 SmaI CCC'GGG Cuts at: 1872 3460 1872 Size: 1588 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2338 2855 3057 4237 5138 5400 Size: 197 926 395 517 202 1180 901 262 Cuts at: 5400 820 Size: 1553 Fragments arranged by size: 1553 1180 926 901 517 395 262 202 197 SnaBI TAC'GTA Cuts at: 493 3924 493 Size: 3431 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6133 SphI G_CATG'C Cuts at: 1132 3491 1132 Size: 2359 3774 SspI AAT'ATT Cuts at: 5 52 4057 5 Size: 47 4005 2081 Fragments arranged by size: 4005 2081 47 StuI AGG'CCT Cuts at: 1517 2034 2427 1517 Size: 517 393 5223 Fragments arranged by size: 5223 517 393 StyI C'CwwG_G Cuts at: 513 1065 2244 2979 3234 3572 513 Size: 552 1179 735 255 338 3074 Fragments arranged by size: 3074 1179 735 552 338 255 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3295 4160 4177 4330 4515 4960 325 Size: 1255 1715 865 17 153 185 445 1498 Fragments arranged by size: 1715 1498 1255 865 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4379 6091 96 Size: 274 80 33 51 1443 2402 1712 138 Fragments arranged by size: 2402 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6093 6125 6093 Size: 32 6101 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6133 XbaI T'CTAG_A Cuts at: 3469 3469 Size: 6133 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2251 2653 3241 2251 Size: 402 588 5143 Fragments arranged by size: 5143 588 402 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6133 XmaI C'CCGG_G Cuts at: 1870 3458 1870 Size: 1588 4545 XmnI GAAnn'nnTTC Cuts at: 4262 4262 Size: 6133 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3012 3941 276 Size: 53 83 186 936 1478 929 2468 Fragments arranged by size: 2468 1478 936 929 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI