Restriction map for pFN21AB0431
 (Circular) (Six-base) MAPSORT of: pFN21AB0431.seq  Check: 5328  from: 1  to: 6133



pFN21AB0431  6133 bp

 With 182 enzymes: SgfI * 

                        January 27, 2011 14:18  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3014    3943     278 
   Size:         53      83     186     936    1478     929    2468

  Fragments arranged by size: 

                2468    1478     936     929     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3476    1937 
   Size:       1539    4594
 
Acc65I G'GTAC_C

Cuts at:   3454    3454 
   Size:       6133
 
AclI AA'CG_TT

Cuts at:   1563    2746    4260    4633    1563 
   Size:       1183    1514     373    3063

  Fragments arranged by size: 

                3063    1514    1183     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3093    4196    5580    5848    1632 
   Size:       1461    1103    1384     268    1917

  Fragments arranged by size: 

                1917    1461    1384    1103     268
 
AflII C'TTAA_G

Cuts at:    820    1017    2855     820 
   Size:        197    1838    4098

  Fragments arranged by size: 

                4098    1838     197
 
AflIII A'CryG_T

Cuts at:   1203    5032    6009    1203 
   Size:       3829     977    1327

  Fragments arranged by size: 

                3829    1327     977
 
AgeI A'CCGG_T

Cuts at:   4964    4964 
   Size:       6133
 
AhdI GACnn_n'nnGTC

Cuts at:   4862    4862 
   Size:       6133
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6133
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6133
 
AlwNI CAG_nnn'CTG

Cuts at:   3876    5448    3876 
   Size:       1572    4561
 
ApaI G_GGCC'C

Cuts at:   2063    2063 
   Size:       6133
 
ApaLI G'TGCA_C

Cuts at:   4190    5346    4190 
   Size:       1156    4977
 
ApoI r'AATT_y

Cuts at:   1493    2694    3442    3668    3820    5929    1493 
   Size:       1201     748     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1201     748     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    2352    4687     160 
   Size:        872    1320    2335    1606

  Fragments arranged by size: 

                2335    1606    1320     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6133
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2062    3458    1870 
   Size:         73     119    1396    4545

  Fragments arranged by size: 

                4545    1396     119      73
 
BamHI G'GATC_C

Cuts at:   3463    3830    3463 
   Size:        367    5766
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3454    4909     618 
   Size:        325     217     627    1667    1455    1842

  Fragments arranged by size: 

                1842    1667    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2063    2631    3003    3118    3223    3343    3452    1391 
   Size:        672     568     372     115     105     120     109    4072

  Fragments arranged by size: 

                4072     672     568     372     120     115     109     105
            
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2579    2624     928 
   Size:        971     680      45    4437

  Fragments arranged by size: 

                4437     971     680      45
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    2678    2712    3378    3412    4324 
   Size:         34      50      34     657      34     666      34     912

Cuts at:   4324    4358    1903 
   Size:         34    3678

  Fragments arranged by size: 

                3678     912     666     657      50      34      34      34
                  34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4025    5235    4025 
   Size:       1210    4923
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6133
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3133    4744     136 
   Size:        107     122      71     631     605    1461    1611    1525

  Fragments arranged by size: 

                1611    1525    1461     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   3053    6128    3053 
   Size:       3075    3058
 
BlpI GC'TnA_GC

Cuts at:   3550    3550 
   Size:       6133
 
Bme1580I G_kGCm'C

Cuts at:   1790    2063    2953    4194    5350    1790 
   Size:        273     890    1241    1156    2573

  Fragments arranged by size: 

                2573    1241    1156     890     273
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6133
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3254    3864    4822 
   Size:        416      53      83     399      24    1837     610     958

Cuts at:   4822     442 
   Size:       1753

  Fragments arranged by size: 

                1837    1753     958     610     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6133
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2276    2946    3014    4793    1239 
   Size:       1037     670      68    1779    2579

  Fragments arranged by size: 

                2579    1779    1037     670      68
 
Bpu10I CC'TnA_GC

Cuts at:   2204    2204 
   Size:       6133
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2323    3042    4258    5123    5421    2323 
   Size:        719    1216     865     298    3035

  Fragments arranged by size: 

                3035    1216     865     719     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4796     882 
   Size:        617    3297    2219

  Fragments arranged by size: 

                3297    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3924     493 
   Size:        711    2720    2702

  Fragments arranged by size: 

                2720    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6127    6127 
   Size:       6133
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2135    3011    3940    4322 
   Size:         53      83     186     936     602     876     929     382

Cuts at:   4322    4957     275 
   Size:        635    1451

  Fragments arranged by size: 

                1451     936     929     876     635     602     382     186
                  83      53
 
BsaWI w'CCGG_w

Cuts at:   2153    4566    4964    5238    5385    5796    2153 
   Size:       2413     398     274     147     411    2490

  Fragments arranged by size: 

                2490    2413     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2066    2096    1178 
   Size:         30     165      30     663      30    5215

  Fragments arranged by size: 

                5215     663     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2201    2273    2737    2740    2922    2927    3011    1186 
   Size:       1015      72     464       3     182       5      84    4308

  Fragments arranged by size: 

                4308    1015     464     182      84      72       5       3
            
 
BseYI C'CCAG_C

Cuts at:   2599    5336    5832    2599 
   Size:       2737     496    2900

  Fragments arranged by size: 

                2900    2737     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2117    2979    1163 
   Size:        573     381     862    4317

  Fragments arranged by size: 

                4317     862     573     381
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2046    3583    4344    5372    1540 
   Size:        275     186      45    1537     761    1028    2301

  Fragments arranged by size: 

                2301    1537    1028     761     275     186      45
 
BsiHKAI G_wGCw'C

Cuts at:   2631    3039    3118    3343    4194    4279    5350    2631 
   Size:        408      79     225     851      85    1071    3414

  Fragments arranged by size: 

                3414    1071     851     408     225      85      79
 
BsmI GAATG_Cn'

Cuts at:   3644    3737    3644 
   Size:         93    6040
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2126    3002    2126 
   Size:        876    5257
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2063    2631    2953    3003    3039    3118    3223 
   Size:        399     273     568     322      50      36      79     105

Cuts at:   3223    3343    3452    4194    4279    5350    1391 
   Size:        120     109     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     568     399     322     273     120
                 109     105      85      79      50      36
 
BspEI T'CCGG_A

Cuts at:   5796    5796 
   Size:       6133
 
BspHI T'CATG_A

Cuts at:   4022    5752    4022 
   Size:       1730    4403
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3488     844 
   Size:       2644    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2067    4020    1394 
   Size:        429     244    1953    3507

  Fragments arranged by size: 

                3507    1953     429     244
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2631    4628    4802      65 
   Size:       1183    1383    1997     174    1396

  Fragments arranged by size: 

                1997    1396    1383    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3309    4777    4964    1700 
   Size:        110    1499    1468     187    2869

  Fragments arranged by size: 

                2869    1499    1468     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6133
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6133
 
BssSI C'ACGA_G

Cuts at:   3122    4193    5205    3122 
   Size:       1071    1012    4050

  Fragments arranged by size: 

                4050    1071    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6133
 
BstBI TT'CG_AA

Cuts at:   4937    4937 
   Size:       6133
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6133
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2980    2980 
   Size:       6133
 
BstYI r'GATC_y

Cuts at:   1972    3053    3378    3463    3830    4215    4232    5684    5770 
   Size:       1081     325      85     367     385      17    1452      86

Cuts at:   5770    5782    6128    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452    1081     385     367     346     325      86
                  85      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2979    3234     513 
   Size:        552    1914     255    3412

  Fragments arranged by size: 

                3412    1914     552     255
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5394

  Fragments arranged by size: 

                5394     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2448    3031    3649    4443    4463    2448 
   Size:        583     618     794      20    4118

  Fragments arranged by size: 

                4118     794     618     583      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6133
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6098
 
DraI TTT'AAA

Cuts at:   3437    3437 
   Size:       6133
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5140     809 
   Size:        507    3824    1802

  Fragments arranged by size: 

                3824    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2043    3181    3311 
   Size:         54    1425      50     275      18     213    1138     130

Cuts at:   3311    3580    4469    6098       8 
   Size:        269     889    1629      43

  Fragments arranged by size: 

                1629    1425    1138     889     275     269     213     130
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2043    3580    1537 
   Size:        275     231    1537    4090

  Fragments arranged by size: 

                4090    1537     275     231
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2186    2562    2697    2751    2770    3244    3410    3417 
   Size:        833     376     135      54      19     474     166       7

Cuts at:   3417    4063    1353 
   Size:        646    3423

  Fragments arranged by size: 

                3423     833     646     474     376     166     135      54
                  19       7
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2210    3381    4720    5094    5240     387 
   Size:       1064     138     621    1171    1339     374     146    1280

  Fragments arranged by size: 

                1339    1280    1171    1064     621     374     146     138
            
 
EcoICRI GAG'CTC

Cuts at:   2629    3116    3341    2629 
   Size:        487     225    5421

  Fragments arranged by size: 

                5421     487     225
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2276    2946    3014    3093    4196    4793    5580 
   Size:        393     644     670      68      79    1103     597     787

Cuts at:   5580    5848    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1103     787     670     644     597     393     268
                  79      68
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2207    2243    2207 
   Size:         36    6097
 
EcoO109I rG'GnC_Cy

Cuts at:   2059    3176    2059 
   Size:       1117    5016
 
EcoRI G'AATT_C

Cuts at:   2694    3442    3820    2694 
   Size:        748     378    5007

  Fragments arranged by size: 

                5007     748     378
 
FspI TGC'GCA

Cuts at:   4639    4639 
   Size:       6133
 
HaeII r_GCGC'y

Cuts at:   1678    5280    1678 
   Size:       3602    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2066    2098    2308    2340    4774 
   Size:         32     135      32     494      32     210      32    2434

Cuts at:   4774    4806    4848    4880    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    2434     494     210     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3477    3723     669 
   Size:        988     281    1539     246    3079

  Fragments arranged by size: 

                3079    1539     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2849     748 
   Size:       2101    4032
 
HpaI GTT'AAC

Cuts at:   3723    3723 
   Size:       6133
 
KpnI G_GTAC'C

Cuts at:   3458    3458 
   Size:       6133
 
MfeI C'AATT_G

Cuts at:   3732    3732 
   Size:       6133
 
MluI A'CGCG_T

Cuts at:   6009    6009 
   Size:       6133
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    5247    5431    1305 
   Size:       3942     184    2007

  Fragments arranged by size: 

                3942    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    3183      10 
   Size:         54    1425     343    1351    2960

  Fragments arranged by size: 

                2960    1425    1351     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3042    3093    4091    4450    4609     518 
   Size:        648    1876      51     998     359     159    2042

  Fragments arranged by size: 

                2042    1876     998     648     359     159      51
 
MspA1I CmG'CkG

Cuts at:   1606    2254    2266    2275    3021    3216    3325    3549    4226 
   Size:        648      12       9     746     195     109     224     677

Cuts at:   4226    5374    5619    1606 
   Size:       1148     245    2120

  Fragments arranged by size: 

                2120    1148     746     677     648     245     224     195
                 109      12       9
 
NcoI C'CATG_G

Cuts at:    513    1065    2979    3234     513 
   Size:        552    1914     255    3412

  Fragments arranged by size: 

                3412    1914     552     255
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6133
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6133
 
NotI GC'GGCC_GC

Cuts at:   3580    3580 
   Size:       6133
 
NruI TCG'CGA

Cuts at:   2184    2184 
   Size:       6133
 
NspI r_CATG'y

Cuts at:   1132    1229    3491    5036    1132 
   Size:         97    2262    1545    2229

  Fragments arranged by size: 

                2262    2229    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6133
 
PciI A'CATG_T

Cuts at:   5032    5032 
   Size:       6133
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6133
 
PfoI T'CCnGG_A

Cuts at:   3334    3334 
   Size:       6133
 
PmeI GTTT'AAAC

Cuts at:   3437    3437 
   Size:       6133
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2635    2667    4202    4234    5715    5747    2635 
   Size:         32    1535      32    1481      32    3021

  Fragments arranged by size: 

                3021    1535    1481      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   3176    3176 
   Size:       6133
 
PshAI GACnn'nnGTC

Cuts at:   1613    2316    3270    1613 
   Size:        703     954    4476

  Fragments arranged by size: 

                4476     954     703
 
PsiI TTA'TAA

Cuts at:   3703    3703 
   Size:       6133
 
PspOMI G'GGCC_C

Cuts at:   2059    2059 
   Size:       6133
 
PstI C_TGCA'G

Cuts at:    830    2443    3485     830 
   Size:       1613    1042    3478

  Fragments arranged by size: 

                3478    1613    1042
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6133
 
PvuII CAG'CTG

Cuts at:   2254    2266    3325    2254 
   Size:         12    1059    5062

  Fragments arranged by size: 

                5062    1059      12
 
SacI G_AGCT'C

Cuts at:   2631    3118    3343    2631 
   Size:        487     225    5421

  Fragments arranged by size: 

                5421     487     225
 
SalI G'TCGA_C

Cuts at:   1936    3475    1936 
   Size:       1539    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   3244    3244 
   Size:       6133
 
SbfI CC_TGCA'GG

Cuts at:   3485    3485 
   Size:       6133
 
ScaI AGT'ACT

Cuts at:   4381    6093    4381 
   Size:       1712    4421
 
SfcI C'TryA_G

Cuts at:    826    1045    2439    3481    4616    5297    5488     826 
   Size:        219    1394    1042    1135     681     191    1471

  Fragments arranged by size: 

                1471    1394    1135    1042     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6133
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6133
 
SmaI CCC'GGG

Cuts at:   1872    3460    1872 
   Size:       1588    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2338    2855    3057    4237    5138    5400 
   Size:        197     926     395     517     202    1180     901     262

Cuts at:   5400     820 
   Size:       1553

  Fragments arranged by size: 

                1553    1180     926     901     517     395     262     202
                 197
 
SnaBI TAC'GTA

Cuts at:    493    3924     493 
   Size:       3431    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6133
 
SphI G_CATG'C

Cuts at:   1132    3491    1132 
   Size:       2359    3774
 
SspI AAT'ATT

Cuts at:      5      52    4057       5 
   Size:         47    4005    2081

  Fragments arranged by size: 

                4005    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2034    2427    1517 
   Size:        517     393    5223

  Fragments arranged by size: 

                5223     517     393
 
StyI C'CwwG_G

Cuts at:    513    1065    2244    2979    3234    3572     513 
   Size:        552    1179     735     255     338    3074

  Fragments arranged by size: 

                3074    1179     735     552     338     255
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3295    4160    4177    4330    4515    4960     325 
   Size:       1255    1715     865      17     153     185     445    1498

  Fragments arranged by size: 

                1715    1498    1255     865     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4379    6091      96 
   Size:        274      80      33      51    1443    2402    1712     138

  Fragments arranged by size: 

                2402    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6093    6125    6093 
   Size:         32    6101
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6133
 
XbaI T'CTAG_A

Cuts at:   3469    3469 
   Size:       6133
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2251    2653    3241    2251 
   Size:        402     588    5143

  Fragments arranged by size: 

                5143     588     402
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6133
 
XmaI C'CCGG_G

Cuts at:   1870    3458    1870 
   Size:       1588    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4262    4262 
   Size:       6133
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3012    3941     276 
   Size:         53      83     186     936    1478     929    2468

  Fragments arranged by size: 

                2468    1478     936     929     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI
     NruI     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI
    PpuMI    PshAI     PsiI   PspOMI     PstI     PvuI    PvuII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI    BsiWI  BstZ17I   Bsu36I   DraIII    EcoRV     FalI
     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NsiI     PacI
     PmlI    PspXI     PsrI    RsrII    SacII    SanDI    SexAI     SfiI
     SfoI     SrfI     SwaI
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