(Linear) (Six-base) MAPSORT of: KIBB0431.seq Check: 6278 from: 1 to: 1428 KIBB0431.orf With 182 enzymes: SgfI * January 27, 2011 13:24 .. AatII G_ACGT'C Cuts at: 0 1009 1428 Size: 1009 419 AclI AA'CG_TT Cuts at: 0 741 1428 Size: 741 687 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1088 1428 Size: 1088 340 AflII C'TTAA_G Cuts at: 0 850 1428 Size: 850 578 ApaI G_GGCC'C Cuts at: 0 58 1428 Size: 58 1370 ApoI r'AATT_y Cuts at: 0 689 1428 Size: 689 739 AseI AT'TA_AT Cuts at: 0 347 1428 Size: 347 1081 AvaI C'yCGr_G Cuts at: 0 57 1428 Size: 57 1371 BanII G_rGCy'C Cuts at: 0 58 626 998 1113 1218 1338 1428 Size: 58 568 372 115 105 120 90 Fragments arranged by size: 568 372 120 115 105 90 58 BbsI GAAGACnn'nnnn_ Cuts at: 0 574 619 1428 Size: 574 45 809 Fragments arranged by size: 809 574 45 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 673 707 1373 1407 1428 Size: 673 34 666 34 21 Fragments arranged by size: 673 666 34 34 21 BglI GCCn_nnn'nGGC Cuts at: 0 1128 1428 Size: 1128 300 BglII A'GATC_T Cuts at: 0 1048 1428 Size: 1048 380 Bme1580I G_kGCm'C Cuts at: 0 58 948 1428 Size: 58 890 480 Fragments arranged by size: 890 480 58 BmrI ACTGGGnnnn_n' Cuts at: 0 1249 1428 Size: 1249 179 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 271 941 1009 1428 Size: 271 670 68 419 Fragments arranged by size: 670 419 271 68 Bpu10I CC'TnA_GC Cuts at: 0 199 1428 Size: 199 1229 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 318 1037 1428 Size: 318 719 391 Fragments arranged by size: 719 391 318 BsaHI Gr'CG_yC Cuts at: 0 130 1006 1428 Size: 130 876 422 Fragments arranged by size: 876 422 130 BsaWI w'CCGG_w Cuts at: 0 148 1428 Size: 148 1280 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 61 91 1428 Size: 61 30 1337 Fragments arranged by size: 1337 61 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 196 268 732 735 917 922 1006 1428 Size: 196 72 464 3 182 5 84 422 Fragments arranged by size: 464 422 196 182 84 72 5 3 BseYI C'CCAG_C Cuts at: 0 594 1428 Size: 594 834 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 112 974 1428 Size: 112 862 454 Fragments arranged by size: 862 454 112 BsiEI CG_ry'CG Cuts at: 0 41 1428 Size: 41 1387 BsiHKAI G_wGCw'C Cuts at: 0 626 1034 1113 1338 1428 Size: 626 408 79 225 90 Fragments arranged by size: 626 408 225 90 79 BsmBI CGTCTCn'nnnn_ Cuts at: 0 121 997 1428 Size: 121 876 431 Fragments arranged by size: 876 431 121 Bsp1286I G_dGCh'C Cuts at: 0 58 626 948 998 1034 1113 1218 1338 Size: 58 568 322 50 36 79 105 120 Cuts at: 1338 1428 Size: 90 Fragments arranged by size: 568 322 120 105 90 79 58 50 36 BsrBI CCG'CTC Cuts at: 0 62 1428 Size: 62 1366 BsrDI GCAATG_nn' Cuts at: 0 626 1428 Size: 626 802 BsrFI r'CCGG_y Cuts at: 0 1304 1428 Size: 1304 124 BssSI C'ACGA_G Cuts at: 0 1117 1428 Size: 1117 311 BstXI CCAn_nnnn'nTGG Cuts at: 0 975 1428 Size: 975 453 BstYI r'GATC_y Cuts at: 0 1048 1373 1428 Size: 1048 325 55 Fragments arranged by size: 1048 325 55 BtgI C'CryG_G Cuts at: 0 974 1229 1428 Size: 974 255 199 Fragments arranged by size: 974 255 199 BtsI GCAGTG_nn' Cuts at: 0 443 1026 1428 Size: 443 583 402 Fragments arranged by size: 583 443 402 EaeI y'GGCC_r Cuts at: 0 38 1176 1306 1428 Size: 38 1138 130 122 Fragments arranged by size: 1138 130 122 38 EagI C'GGCC_G Cuts at: 0 38 1428 Size: 38 1390 EarI CTCTTCn'nnn_ Cuts at: 0 181 557 692 746 765 1239 1405 1412 Size: 181 376 135 54 19 474 166 7 Cuts at: 1412 1428 Size: 16 Fragments arranged by size: 474 376 181 166 135 54 19 16 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 205 1376 1428 Size: 205 1171 52 Fragments arranged by size: 1171 205 52 EcoICRI GAG'CTC Cuts at: 0 624 1111 1336 1428 Size: 624 487 225 92 Fragments arranged by size: 624 487 225 92 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 271 941 1009 1088 1428 Size: 271 670 68 79 340 Fragments arranged by size: 670 340 271 79 68 EcoNI CCTnn'n_nnAGG Cuts at: 0 202 238 1428 Size: 202 36 1190 Fragments arranged by size: 1190 202 36 EcoO109I rG'GnC_Cy Cuts at: 0 54 1171 1428 Size: 54 1117 257 Fragments arranged by size: 1117 257 54 EcoRI G'AATT_C Cuts at: 0 689 1428 Size: 689 739 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 61 93 303 335 1428 Size: 61 32 210 32 1093 Fragments arranged by size: 1093 210 61 32 32 HindIII A'AGCT_T Cuts at: 0 844 1428 Size: 844 584 MscI TGG'CCA Cuts at: 0 1178 1428 Size: 1178 250 MslI CAynn'nnrTG Cuts at: 0 1037 1088 1428 Size: 1037 51 340 Fragments arranged by size: 1037 340 51 MspA1I CmG'CkG Cuts at: 0 249 261 270 1016 1211 1320 1428 Size: 249 12 9 746 195 109 108 Fragments arranged by size: 746 249 195 109 108 12 9 NcoI C'CATG_G Cuts at: 0 974 1229 1428 Size: 974 255 199 Fragments arranged by size: 974 255 199 NruI TCG'CGA Cuts at: 0 179 1428 Size: 179 1249 PfoI T'CCnGG_A Cuts at: 0 1329 1428 Size: 1329 99 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 630 662 1428 Size: 630 32 766 Fragments arranged by size: 766 630 32 PpuMI rG'GwC_Cy Cuts at: 0 1171 1428 Size: 1171 257 PshAI GACnn'nnGTC Cuts at: 0 311 1265 1428 Size: 311 954 163 Fragments arranged by size: 954 311 163 PspOMI G'GGCC_C Cuts at: 0 54 1428 Size: 54 1374 PstI C_TGCA'G Cuts at: 0 438 1428 Size: 438 990 PvuII CAG'CTG Cuts at: 0 249 261 1320 1428 Size: 249 12 1059 108 Fragments arranged by size: 1059 249 108 12 SacI G_AGCT'C Cuts at: 0 626 1113 1338 1428 Size: 626 487 225 90 Fragments arranged by size: 626 487 225 90 SapI GCTCTTCn'nnn_ Cuts at: 0 1239 1428 Size: 1239 189 SfcI C'TryA_G Cuts at: 0 434 1428 Size: 434 994 SmlI C'TyrA_G Cuts at: 0 333 850 1052 1428 Size: 333 517 202 376 Fragments arranged by size: 517 376 333 202 StuI AGG'CCT Cuts at: 0 29 422 1428 Size: 29 393 1006 Fragments arranged by size: 1006 393 29 StyI C'CwwG_G Cuts at: 0 239 974 1229 1428 Size: 239 735 255 199 Fragments arranged by size: 735 255 239 199 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1290 1428 Size: 1290 138 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 246 648 1236 1428 Size: 246 402 588 192 Fragments arranged by size: 588 402 246 192 ZraI GAC'GTC Cuts at: 0 1007 1428 Size: 1007 421 Enzymes that do cut: AatII AclI AcuI AflII ApaI ApoI AseI AvaI BanII BbsI BcgI BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BsrFI BssSI BstXI BstYI BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I HindIII MscI MslI MspA1I NcoI NruI PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SacI SapI SfcI SmlI StuI StyI TaqII XcmI ZraI Enzymes that do not cut: AarI AccI Acc65I AfeI AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BciVI BclI BfrBI BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsiWI BsmI BspEI BspHI BspMI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoRV FalI FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI