################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AccI AccIII AclI AcsI AflII AgeI Alw44I ApaLI ApoI AseI AsnI Asp700I AvrII BamHI BbrPI BbsI BcgI BclI BfrI BlnI BpuAI BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI EcoRI EcoRV EcoT22I FbaI HindIII HpaI MamI MfeI MluI MroI MseI MunI NdeI NheI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SfuI SnaBI SpeI SspBI SspI SwaI TfiI Tru9I Tsp509I XbaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AatII 3303 GACGTC GACGT^C Acc65I 404 GGTACC G^GTACC AflIII 2303 ACGTGT A^C[GA][CT]GT AlwI 3706 GGATC GGATC(4/5) AocI 2521 CCTCAGG CC^T[ACGT]AGG AscI 259 GGCGCGCC GG^CGCGCC Asp718I 404 GGTACC G^GTACC BbvCI 3826 CCTCAGC CCTCAGC(-5/-2) BciVI 3039 GTATCC GTATCC(6/5) BfaI 2615 CTAG C^TAG BglII 2063 AGATCT A^GATCT BlpI 3321 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3321 GCTGAGC GC^T[ACGT]AGC BsaAI 2467 TACGTG [CT]AC^GT[GA] BsaWI 2730 ACCGGA [AT]^CCGG[AT] BsrI 2463 CCAGT ACTGG(1/-1) BstYI 2063 AGATCT [GA]^GATC[CT] Bsu36I 2521 CCTCAGG CC^T[ACGT]AGG CelII 3321 GCTGAGC GC^T[ACGT]AGC Eco81I 2521 CCTCAGG CC^T[ACGT]AGG EcoNI 2499 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HincII 985 GTCAAC GT[CT]^[GA]AC HindII 985 GTCAAC GT[CT]^[GA]AC KpnI 404 GGTACC GGTAC^C MaeI 2615 CTAG C^TAG MflI 2063 AGATCT [GA]^GATC[CT] PaeR7I 217 CTCGAG C^TCGAG PvuII 2004 CAGCTG CAG^CTG SapI 888 GCTCTTC GCTCTTC(1/4) SbfI 3360 CCTGCAGG CCTGCA^GG Sse8387I 3360 CCTGCAGG CCTGCA^GG XhoI 217 CTCGAG C^TCGAG XhoII 2063 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AhdI 3660 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3970 GACTTCTTGTC AlwNI 1693 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 3278 CAGAAGCTG Aor51HI 134 AGCGCT AGC^GCT 3883 AGCGCT AspEI 3660 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3970 GACTTCTTGTC AspI 421 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2317 GACGCGGTC BalI 126 TGGCCA TGG^CCA 3293 TGGCCA BspHI 725 TCATGA T^CATGA 2474 TCATGA BstEII 2322 GGTCACC G^GT[ACGT]ACC 3471 GGTCACC BstPI 2322 GGTCACC G^GT[ACGT]ACC 3471 GGTCACC BstXI 611 CCAAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1295 CCATGCCCGTGG DraIII 85 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG 2989 CACCCCGTG Eam1105I 3660 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3970 GACTTCTTGTC EarI 889 CTCTTC CTCTTC(1/4) 3315 GAAGAG Eco47III 134 AGCGCT AGC^GCT 3883 AGCGCT EcoO65I 2322 GGTCACC G^GT[ACGT]ACC 3471 GGTCACC FseI 2565 GGCCGGCC GGCCGG^CC 3649 GGCCGGCC Ksp632I 889 CTCTTC CTCTTC(1/4) 3315 GAAGAG MluNI 126 TGGCCA TGG^CCA 3293 TGGCCA MscI 126 TGGCCA TGG^CCA 3293 TGGCCA NcoI 1631 CCATGG C^CATGG 2255 CCATGG PflFI 421 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2317 GACGCGGTC RcaI 725 TCATGA T^CATGA 2474 TCATGA ScaI 221 AGTACT AGT^ACT 359 AGTACT SexAI 422 ACCTGGT A^CC[AT]GGT 980 ACCTGGT SgrAI 1052 CGCCGGCG C[GA]^CCGG[CT]G 3903 CACCGGCG SmlI 217 CTCGAG C^T[CT][GA]AG 880 CTCAAG SphI 2262 GCATGC GCATG^C 2708 GCATGC StuI 3026 AGGCCT AGG^CCT 3353 AGGCCT Tth111I 421 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2317 GACGCGGTC XcmI 1928 CCAGCAGCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2246 CCAAGCTGTCCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccII AciI AcyI AfaI AluI ApaI AspHI AvaI AvaII AviII BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BssHII BssKI BssSI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NotI NspI PflMI PleI PpuMI PstI RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SfoI SmaI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XmaCI XmaI