(Circular) (Six-base) MAPSORT of: pFN21AE2308.seq Check: 2746 from: 1 to: 8719 pFN21AE2308 8719 bp With 182 enzymes: SgfI * December 8, 2014 13:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2590 4457 5332 2590 Size: 1867 875 5977 Fragments arranged by size: 5977 1867 875 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5312 6529 278 Size: 53 83 186 936 3776 1217 2468 Fragments arranged by size: 3776 2468 1217 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6062 1937 Size: 4125 4594 Acc65I G'GTAC_C Cuts at: 2409 6040 2409 Size: 3631 5088 AclI AA'CG_TT Cuts at: 1563 6846 7219 1563 Size: 5283 373 3063 Fragments arranged by size: 5283 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2630 4936 6782 8166 8434 1632 Size: 998 2306 1846 1384 268 1917 Fragments arranged by size: 2306 1917 1846 1384 998 268 AfeI AGC'GCT Cuts at: 2141 5890 2141 Size: 3749 4970 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8522 AflIII A'CryG_T Cuts at: 1203 4308 7618 8595 1203 Size: 3105 3310 977 1327 Fragments arranged by size: 3310 3105 1327 977 AgeI A'CCGG_T Cuts at: 7550 7550 Size: 8719 AhdI GACnn_n'nnGTC Cuts at: 5670 5980 7448 5670 Size: 310 1468 6941 Fragments arranged by size: 6941 1468 310 AleI CACnn'nnGTG Cuts at: 1166 5483 6008 1166 Size: 4317 525 3877 Fragments arranged by size: 4317 3877 525 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4301 1184 Size: 3117 5602 AlwNI CAG_nnn'CTG Cuts at: 3703 5288 6462 8034 3703 Size: 1585 1174 1572 4388 Fragments arranged by size: 4388 1585 1572 1174 ApaI G_GGCC'C Cuts at: 4545 4792 5171 4545 Size: 247 379 8093 Fragments arranged by size: 8093 379 247 ApaLI G'TGCA_C Cuts at: 6776 7932 6776 Size: 1156 7563 ApoI r'AATT_y Cuts at: 1493 6028 6254 6406 8515 1493 Size: 4535 226 152 2109 1697 Fragments arranged by size: 4535 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 2265 2265 Size: 8719 AseI AT'TA_AT Cuts at: 160 1032 7273 160 Size: 872 6241 1606 Fragments arranged by size: 6241 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8719 AvaI C'yCGr_G Cuts at: 1870 1943 2033 2222 3179 3370 3469 3566 3594 Size: 73 90 189 957 191 99 97 28 Cuts at: 3594 3682 3820 4214 4784 4891 4984 5027 5116 Size: 88 138 394 570 107 93 43 89 Cuts at: 5116 5170 5668 5678 5789 5873 6044 1870 Size: 54 498 10 111 84 171 4545 Fragments arranged by size: 4545 957 570 498 394 191 189 171 138 111 107 99 97 93 90 89 88 84 73 54 43 28 10 BamHI G'GATC_C Cuts at: 6049 6416 6049 Size: 367 8352 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2203 2409 2510 2531 3140 Size: 325 217 627 416 206 101 21 609 Cuts at: 3140 3763 4337 4886 4895 4979 5174 5399 5632 Size: 623 574 549 9 84 195 225 233 Cuts at: 5632 5644 5939 6040 7495 618 Size: 12 295 101 1455 1842 Fragments arranged by size: 1842 1455 627 623 609 574 549 416 325 295 233 225 217 206 195 101 101 84 21 12 9 BanII G_rGCy'C Cuts at: 1391 2015 2895 3170 3179 3377 3476 3932 4043 Size: 624 880 275 9 198 99 456 111 Cuts at: 4043 4211 4487 4545 4743 4751 4785 4792 4883 Size: 168 276 58 198 8 34 7 91 Cuts at: 4883 5006 5171 5852 6038 1391 Size: 123 165 681 186 4072 Fragments arranged by size: 4072 880 681 624 456 276 275 198 198 186 168 165 123 111 99 91 58 34 9 8 7 BbeI G_GCGC'C Cuts at: 2207 2514 2535 3144 4983 5178 5403 5636 2207 Size: 307 21 609 1839 195 225 233 5290 Fragments arranged by size: 5290 1839 609 307 233 225 195 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 7748 BbvCI CC'TCA_GC Cuts at: 5832 5832 Size: 8719 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6910 6944 1903 Size: 34 4973 34 3678 Fragments arranged by size: 4973 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5055 6611 7821 5055 Size: 1556 1210 5953 Fragments arranged by size: 5953 1556 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8719 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2035 2540 2748 Size: 107 122 71 631 605 363 505 208 Cuts at: 2748 2879 3012 3660 3751 4129 4568 4615 4638 Size: 131 133 648 91 378 439 47 23 Cuts at: 4638 4656 5297 7330 136 Size: 18 641 2033 1525 Fragments arranged by size: 2033 1525 648 641 631 605 505 439 378 363 208 133 131 122 107 91 71 47 23 18 BglII A'GATC_T Cuts at: 4068 8714 4068 Size: 4646 4073 BlpI GC'TnA_GC Cuts at: 5327 6136 5327 Size: 809 7910 Bme1580I G_kGCm'C Cuts at: 1790 3768 4340 4545 4792 4898 5171 5944 6780 Size: 1978 572 205 247 106 273 773 836 Cuts at: 6780 7936 1790 Size: 1156 2573 Fragments arranged by size: 2573 1978 1156 836 773 572 273 247 205 106 BmgBI CAC'GTC Cuts at: 1318 5776 1318 Size: 4458 4261 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6450 7408 442 Size: 416 53 83 399 24 5033 958 1753 Fragments arranged by size: 5033 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8719 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3532 3934 5053 5476 3532 Size: 402 1119 423 6775 Fragments arranged by size: 6775 1119 423 402 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2888 3433 3502 3916 4526 4541 4709 5607 Size: 1649 545 69 414 610 15 168 898 Cuts at: 5607 7379 1239 Size: 1772 2579 Fragments arranged by size: 2579 1772 1649 898 610 545 414 168 69 15 Bpu10I CC'TnA_GC Cuts at: 3173 3200 3218 3650 4388 4882 4971 5832 3173 Size: 27 18 432 738 494 89 861 6060 Fragments arranged by size: 6060 861 738 494 432 89 27 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2870 6844 7709 8007 2870 Size: 3974 865 298 3582 Fragments arranged by size: 3974 3582 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2852 3685 4926 5447 5723 5759 5885 Size: 617 1353 833 1241 521 276 36 126 Cuts at: 5885 7382 882 Size: 1497 2219 Fragments arranged by size: 2219 1497 1353 1241 833 617 521 276 126 36 BsaAI yAC'GTr Cuts at: 493 1204 4474 6510 493 Size: 711 3270 2036 2702 Fragments arranged by size: 3270 2702 2036 711 BsaBI GATnn'nnATC Cuts at: 8713 8713 Size: 8719 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2204 2322 2511 2532 Size: 53 83 186 936 671 118 189 21 Cuts at: 2532 3141 3584 3974 4823 4980 5175 5309 5400 Size: 609 443 390 849 157 195 134 91 Cuts at: 5400 5633 5898 6526 6908 7543 275 Size: 233 265 628 382 635 1451 Fragments arranged by size: 1451 936 849 671 635 628 609 443 390 382 265 233 195 189 186 157 134 118 91 83 53 21 BsaWI w'CCGG_w Cuts at: 4735 7152 7550 7824 7971 8382 4735 Size: 2417 398 274 147 411 5072 Fragments arranged by size: 5072 2417 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2393 2423 3275 3305 3914 Size: 30 165 30 990 30 852 30 609 Cuts at: 3914 3944 4340 4370 4940 4970 5330 5360 5513 Size: 30 396 30 570 30 360 30 153 Cuts at: 5513 5543 5834 5864 1178 Size: 30 291 30 4033 Fragments arranged by size: 4033 990 852 609 570 396 360 291 165 153 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3766 4523 4526 4831 5026 5452 1186 Size: 2580 757 3 305 195 426 4453 Fragments arranged by size: 4453 2580 757 426 305 195 3 BseYI C'CCAG_C Cuts at: 3002 3161 3479 3768 4316 4544 4700 4709 4876 Size: 159 318 289 548 228 156 9 167 Cuts at: 4876 5802 7922 8418 3002 Size: 926 2120 496 3303 Fragments arranged by size: 3303 2120 926 548 496 318 289 228 167 159 156 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3091 3407 4352 1163 Size: 573 1355 316 945 5530 Fragments arranged by size: 5530 1355 945 573 316 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2451 2580 2793 2909 3135 3238 Size: 275 186 450 129 213 116 226 103 Cuts at: 3238 3761 4630 4660 5151 5660 6169 6930 7958 Size: 523 869 30 491 509 509 761 1028 Cuts at: 7958 1540 Size: 2301 Fragments arranged by size: 2301 1028 869 761 523 509 509 491 450 275 226 213 186 129 116 103 30 BsiHKAI G_wGCw'C Cuts at: 2895 3932 4211 4479 4487 5852 6780 6865 7936 Size: 1037 279 268 8 1365 928 85 1071 Cuts at: 7936 2895 Size: 3678 Fragments arranged by size: 3678 1365 1071 1037 928 279 268 85 8 BsmI GAATG_Cn' Cuts at: 6230 6323 6230 Size: 93 8626 BsmBI CGTCTCn'nnnn_ Cuts at: 2043 2451 3575 3591 5091 2043 Size: 408 1124 16 1500 5671 Fragments arranged by size: 5671 1500 1124 408 16 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2895 3170 3179 3377 3476 3768 Size: 399 225 880 275 9 198 99 292 Cuts at: 3768 3932 4043 4211 4340 4479 4487 4545 4743 Size: 164 111 168 129 139 8 58 198 Cuts at: 4743 4751 4785 4792 4883 4898 5006 5171 5852 Size: 8 34 7 91 15 108 165 681 Cuts at: 5852 5944 6038 6780 6865 7936 1391 Size: 92 94 742 85 1071 2174 Fragments arranged by size: 2174 1071 880 742 681 399 292 275 225 198 198 168 165 164 139 129 111 108 99 94 92 91 85 58 34 15 9 8 8 7 BspEI T'CCGG_A Cuts at: 8382 8382 Size: 8719 BspHI T'CATG_A Cuts at: 2730 4479 6608 8338 2730 Size: 1749 2129 1730 3111 Fragments arranged by size: 3111 2129 1749 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2121 2590 3003 3540 4457 5332 6074 844 Size: 1277 469 413 537 917 875 742 3489 Fragments arranged by size: 3489 1277 917 875 742 537 469 413 BsrBI CCG'CTC Cuts at: 1394 1823 2046 2787 3027 3128 3207 3222 3291 Size: 429 223 741 240 101 79 15 69 Cuts at: 3291 3666 4005 4387 4428 4927 4989 5706 6606 Size: 375 339 382 41 499 62 717 900 Cuts at: 6606 1394 Size: 3507 Fragments arranged by size: 3507 900 741 717 499 429 382 375 339 240 223 101 79 69 62 41 15 BsrDI GCAATG_nn' Cuts at: 65 1248 7214 7388 65 Size: 1183 5966 174 1396 Fragments arranged by size: 5966 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2038 2528 3058 3148 3184 3262 3760 Size: 110 228 490 530 90 36 78 498 Cuts at: 3760 4571 4911 5110 5629 5655 5738 5909 7363 Size: 811 340 199 519 26 83 171 1454 Cuts at: 7363 7550 1700 Size: 187 2869 Fragments arranged by size: 2869 1454 811 530 519 498 490 340 228 199 187 171 110 90 83 78 36 26 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8719 BssHII G'CGCG_C Cuts at: 1925 2265 2314 2415 2672 2967 2969 3190 3192 Size: 340 49 101 257 295 2 221 2 Cuts at: 3192 3273 3535 1925 Size: 81 262 7109 Fragments arranged by size: 7109 340 295 262 257 221 101 81 49 2 2 BssSI C'ACGA_G Cuts at: 2855 3076 4718 6779 7791 2855 Size: 221 1642 2061 1012 3783 Fragments arranged by size: 3783 2061 1642 1012 221 BstAPI GCAn_nnn'nTGC Cuts at: 794 3078 3421 4072 4242 794 Size: 2284 343 651 170 5271 Fragments arranged by size: 5271 2284 651 343 170 BstBI TT'CG_AA Cuts at: 7523 7523 Size: 8719 BstEII G'GTnAC_C Cuts at: 1184 4327 5476 1184 Size: 3143 1149 4427 Fragments arranged by size: 4427 3143 1149 BstXI CCAn_nnnn'nTGG Cuts at: 2623 3307 2623 Size: 684 8035 BstYI r'GATC_y Cuts at: 1972 4068 6049 6416 6801 6818 8270 8356 8368 Size: 2096 1981 367 385 17 1452 86 12 Cuts at: 8368 8714 1972 Size: 346 1977 Fragments arranged by size: 2096 1981 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4527 4527 Size: 8719 BtgI C'CryG_G Cuts at: 513 1065 2004 2018 2216 2373 2574 3135 3238 Size: 552 939 14 198 157 201 561 103 Cuts at: 3238 3306 3364 3427 3636 4260 4332 4491 4998 Size: 68 58 63 209 624 72 159 507 Cuts at: 4998 5395 5532 5660 513 Size: 397 137 128 3572 Fragments arranged by size: 3572 939 624 561 552 507 397 209 201 198 159 157 137 128 103 72 68 63 58 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3559 5749 487 Size: 683 56 2333 2190 3457 Fragments arranged by size: 3457 2333 2190 683 56 BtsI GCAGTG_nn' Cuts at: 4003 4727 6235 7029 7049 4003 Size: 724 1508 794 20 5673 Fragments arranged by size: 5673 1508 794 724 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8719 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5604 5639 529 Size: 35 5040 35 3609 Fragments arranged by size: 5040 3609 35 35 DraI TTT'AAA Cuts at: 6023 6023 Size: 8719 DraIII CAC_nnn'GTG Cuts at: 2095 4999 2095 Size: 2904 5815 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7726 809 Size: 507 6410 1802 Fragments arranged by size: 6410 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2131 2376 2448 Size: 54 1425 50 275 18 301 245 72 Cuts at: 2448 2577 2749 2790 2906 2940 3045 3063 3132 Size: 129 172 41 116 34 105 18 69 Cuts at: 3132 3235 3361 3436 3661 3758 4554 4627 4657 Size: 103 126 75 225 97 796 73 30 Cuts at: 4657 4990 5148 5241 5298 5657 6166 7055 8684 Size: 333 158 93 57 359 509 889 1629 Cuts at: 8684 8 Size: 43 Fragments arranged by size: 1629 1425 889 796 509 359 333 301 275 245 225 172 158 129 126 116 105 103 97 93 75 73 72 69 57 54 50 43 41 34 30 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2448 2577 2790 2906 3132 3235 3758 Size: 275 636 129 213 116 226 103 523 Cuts at: 3758 4627 4657 5148 5657 6166 1537 Size: 869 30 491 509 509 4090 Fragments arranged by size: 4090 869 636 523 509 509 491 275 226 213 129 116 103 30 EarI CTCTTCn'nnn_ Cuts at: 1353 2900 5315 6649 1353 Size: 1547 2415 1334 3423 Fragments arranged by size: 3423 2415 1547 1334 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2266 2729 2840 3454 4045 4843 Size: 1064 138 677 463 111 614 591 798 Cuts at: 4843 5037 7306 7680 7826 387 Size: 194 2269 374 146 1280 Fragments arranged by size: 2269 1280 1064 798 677 614 591 463 374 194 146 138 111 EcoICRI GAG'CTC Cuts at: 2893 3930 4209 4485 5850 2893 Size: 1037 279 276 1365 5762 Fragments arranged by size: 5762 1365 1037 279 276 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2630 2888 3433 3502 3916 4526 4541 Size: 393 998 258 545 69 414 610 15 Cuts at: 4541 4709 4936 5607 6782 7379 8166 8434 1239 Size: 168 227 671 1175 597 787 268 1524 Fragments arranged by size: 1524 1175 998 787 671 610 597 545 414 393 268 258 227 168 69 15 EcoNI CCTnn'n_nnAGG Cuts at: 4508 4508 Size: 8719 EcoO109I rG'GnC_Cy Cuts at: 2801 3645 3700 3726 4121 4438 4541 4788 5167 Size: 844 55 26 395 317 103 247 379 Cuts at: 5167 5933 2801 Size: 766 5587 Fragments arranged by size: 5587 844 766 395 379 317 247 103 55 26 EcoRI G'AATT_C Cuts at: 6028 6406 6028 Size: 378 8341 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2161 2911 4087 2161 Size: 750 1176 6793 Fragments arranged by size: 6793 1176 750 FseI GG_CCGG'CC Cuts at: 4575 5659 4575 Size: 1084 7635 FspI TGC'GCA Cuts at: 2099 2117 3089 3719 4235 5723 7225 2099 Size: 18 972 630 516 1488 1502 3593 Fragments arranged by size: 3593 1502 1488 972 630 516 18 FspAI rTGC'GCAy Cuts at: 3089 4235 3089 Size: 1146 7573 HaeII r_GCGC'y Cuts at: 1678 2143 2207 2499 2514 2535 2885 3144 3257 Size: 465 64 292 15 21 350 259 113 Cuts at: 3257 3462 4563 4983 5178 5403 5518 5636 5892 Size: 205 1101 420 195 225 115 118 256 Cuts at: 5892 7866 1678 Size: 1974 2531 Fragments arranged by size: 2531 1974 1101 465 420 350 292 259 256 225 205 195 118 115 113 64 21 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2696 2728 4340 4372 4940 Size: 32 135 32 1124 32 1612 32 568 Cuts at: 4940 4972 5330 5362 5656 5688 5834 5866 5966 Size: 32 358 32 294 32 146 32 100 Cuts at: 5966 5998 7360 7392 7434 7466 1373 Size: 32 1362 32 42 32 2626 Fragments arranged by size: 2626 1612 1362 1124 568 358 294 146 135 100 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2992 6063 6309 669 Size: 988 281 1054 3071 246 3079 Fragments arranged by size: 3079 3071 1054 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8719 HpaI GTT'AAC Cuts at: 6309 6309 Size: 8719 KasI G'GCGC_C Cuts at: 2203 2510 2531 3140 4979 5174 5399 5632 2203 Size: 307 21 609 1839 195 225 233 5290 Fragments arranged by size: 5290 1839 609 307 233 225 195 21 KpnI G_GTAC'C Cuts at: 2413 6044 2413 Size: 3631 5088 MfeI C'AATT_G Cuts at: 6318 6318 Size: 8719 MluI A'CGCG_T Cuts at: 8595 8595 Size: 8719 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4956 7833 8017 1305 Size: 3651 2877 184 2007 Fragments arranged by size: 3651 2877 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2133 5300 10 Size: 54 1425 343 301 3167 3429 Fragments arranged by size: 3429 3167 1425 343 301 54 MslI CAynn'nnrTG Cuts at: 518 1166 2777 3299 3305 3635 3803 3968 4034 Size: 648 1611 522 6 330 168 165 66 Cuts at: 4034 4235 4272 5225 5483 5774 6008 6677 7036 Size: 201 37 953 258 291 234 669 359 Cuts at: 7036 7195 518 Size: 159 2042 Fragments arranged by size: 2042 1611 953 669 648 522 359 330 291 258 234 201 168 165 159 66 37 6 MspA1I CmG'CkG Cuts at: 1606 2020 2218 2745 3137 3240 3360 3421 3442 Size: 414 198 527 392 103 120 61 21 Cuts at: 3442 3453 3757 4011 4918 5397 6135 6812 7960 Size: 11 304 254 907 479 738 677 1148 Cuts at: 7960 8205 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 907 738 677 527 479 414 392 304 254 245 198 120 103 61 21 11 NaeI GCC'GGC Cuts at: 2040 2530 3060 3264 4573 4913 5112 5631 5657 Size: 490 530 204 1309 340 199 519 26 Cuts at: 5657 5740 2040 Size: 83 5019 Fragments arranged by size: 5019 1309 530 519 490 340 204 199 83 26 NarI GG'CG_CC Cuts at: 2204 2511 2532 3141 4980 5175 5400 5633 2204 Size: 307 21 609 1839 195 225 233 5290 Fragments arranged by size: 5290 1839 609 307 233 225 195 21 NcoI C'CATG_G Cuts at: 513 1065 2004 3636 4260 513 Size: 552 939 1632 624 4972 Fragments arranged by size: 4972 1632 939 624 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8719 NgoMIV G'CCGG_C Cuts at: 2038 2528 3058 3262 4571 4911 5110 5629 5655 Size: 490 530 204 1309 340 199 519 26 Cuts at: 5655 5738 2038 Size: 83 5019 Fragments arranged by size: 5019 1309 530 519 490 340 204 199 83 26 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8719 NotI GC'GGCC_GC Cuts at: 2448 3132 3235 4627 6166 2448 Size: 684 103 1392 1539 5001 Fragments arranged by size: 5001 1539 1392 684 103 NspI r_CATG'y Cuts at: 1132 1229 3967 4033 4234 4240 4271 4717 6077 Size: 97 2738 66 201 6 31 446 1360 Cuts at: 6077 7622 1132 Size: 1545 2229 Fragments arranged by size: 2738 2229 1545 1360 446 201 97 66 31 6 PasI CC'CwG_GG Cuts at: 1348 2515 4080 5164 1348 Size: 1167 1565 1084 4903 Fragments arranged by size: 4903 1565 1167 1084 PciI A'CATG_T Cuts at: 7618 7618 Size: 8719 PflMI CCAn_nnn'nTGG Cuts at: 1108 2986 3703 4999 1108 Size: 1878 717 1296 4828 Fragments arranged by size: 4828 1878 1296 717 PfoI T'CCnGG_A Cuts at: 2865 4503 2865 Size: 1638 7081 PmeI GTTT'AAAC Cuts at: 6023 6023 Size: 8719 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6788 6820 8301 8333 6788 Size: 32 1481 32 7174 Fragments arranged by size: 7174 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3700 3726 5933 3700 Size: 26 2207 6486 Fragments arranged by size: 6486 2207 26 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8719 PsiI TTA'TAA Cuts at: 6289 6289 Size: 8719 PspOMI G'GGCC_C Cuts at: 4541 4788 5167 4541 Size: 247 379 8093 Fragments arranged by size: 8093 379 247 PspXI vC'TCGA_Gb Cuts at: 2222 2222 Size: 8719 PstI C_TGCA'G Cuts at: 830 2495 2727 3548 4016 5094 5370 6071 830 Size: 1665 232 821 468 1078 276 701 3478 Fragments arranged by size: 3478 1665 1078 821 701 468 276 232 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8719 PvuII CAG'CTG Cuts at: 4011 4011 Size: 8719 SacI G_AGCT'C Cuts at: 2895 3932 4211 4487 5852 2895 Size: 1037 279 276 1365 5762 Fragments arranged by size: 5762 1365 1037 279 276 SacII CC_GC'GG Cuts at: 2021 2219 3138 3241 5398 2021 Size: 198 919 103 2157 5342 Fragments arranged by size: 5342 2157 919 198 103 SalI G'TCGA_C Cuts at: 1936 6061 1936 Size: 4125 4594 SanDI GG'GwC_CC Cuts at: 5933 5933 Size: 8719 SapI GCTCTTCn'nnn_ Cuts at: 2900 2900 Size: 8719 SbfI CC_TGCA'GG Cuts at: 5370 6071 5370 Size: 701 8018 ScaI AGT'ACT Cuts at: 2228 2366 6967 8679 2228 Size: 138 4601 1712 2268 Fragments arranged by size: 4601 2268 1712 138 SexAI A'CCwGG_T Cuts at: 2427 2985 2427 Size: 558 8161 SfcI C'TryA_G Cuts at: 826 1045 2491 2723 3544 4012 4411 4596 5090 Size: 219 1446 232 821 468 399 185 494 Cuts at: 5090 5366 6067 7202 7883 8074 826 Size: 276 701 1135 681 191 1471 Fragments arranged by size: 1471 1446 1135 821 701 681 494 468 399 276 232 219 191 185 SfiI GGCCn_nnn'nGGCC Cuts at: 2035 4129 4656 2035 Size: 2094 527 6098 Fragments arranged by size: 6098 2094 527 SfoI GGC'GCC Cuts at: 2205 2512 2533 3142 4981 5176 5401 5634 2205 Size: 307 21 609 1839 195 225 233 5290 Fragments arranged by size: 5290 1839 609 307 233 225 195 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8719 SgrAI Cr'CCGG_yG Cuts at: 1700 3058 5909 1700 Size: 1358 2851 4510 Fragments arranged by size: 4510 2851 1358 SmaI CCC'GGG Cuts at: 1872 2035 3181 4786 4893 5118 5172 5670 5680 Size: 163 1146 1605 107 225 54 498 10 Cuts at: 5680 5791 6046 1872 Size: 111 255 4545 Fragments arranged by size: 4545 1605 1146 498 255 225 163 111 107 54 10 SmlI C'TyrA_G Cuts at: 820 1017 1943 2222 2885 6823 7724 7986 820 Size: 197 926 279 663 3938 901 262 1553 Fragments arranged by size: 3938 1553 926 901 663 279 262 197 SnaBI TAC'GTA Cuts at: 493 6510 493 Size: 6017 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8719 SphI G_CATG'C Cuts at: 1132 4271 4717 6077 1132 Size: 3139 446 1360 3774 Fragments arranged by size: 3774 3139 1360 446 SrfI GCCC'GGGC Cuts at: 2035 5172 2035 Size: 3137 5582 SspI AAT'ATT Cuts at: 5 52 6643 5 Size: 47 6591 2081 Fragments arranged by size: 6591 2081 47 StuI AGG'CCT Cuts at: 1517 5033 5360 1517 Size: 3516 327 4876 Fragments arranged by size: 4876 3516 327 StyI C'CwwG_G Cuts at: 513 1065 2004 2355 2616 3636 3867 4260 5007 Size: 552 939 351 261 1020 231 393 747 Cuts at: 5007 5403 6158 513 Size: 396 755 3074 Fragments arranged by size: 3074 1020 939 755 747 552 396 393 351 261 231 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3723 4153 5564 6746 6763 6916 7101 Size: 1255 2143 430 1411 1182 17 153 185 Cuts at: 7101 7546 325 Size: 445 1498 Fragments arranged by size: 2143 1498 1411 1255 1182 445 430 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2226 2364 4758 Size: 274 80 33 51 1443 249 138 2394 Cuts at: 4758 6965 8677 96 Size: 2207 1712 138 Fragments arranged by size: 2394 2207 1712 1443 274 249 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3275 3307 3914 3946 8679 8711 3275 Size: 32 607 32 4733 32 3283 Fragments arranged by size: 4733 3283 607 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2429 4325 1316 Size: 1113 1896 5710 Fragments arranged by size: 5710 1896 1113 XbaI T'CTAG_A Cuts at: 6055 6055 Size: 8719 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3940 4258 3940 Size: 318 8401 XhoI C'TCGA_G Cuts at: 1943 2222 1943 Size: 279 8440 XmaI C'CCGG_G Cuts at: 1870 2033 3179 4784 4891 5116 5170 5668 5678 Size: 163 1146 1605 107 225 54 498 10 Cuts at: 5678 5789 6044 1870 Size: 111 255 4545 Fragments arranged by size: 4545 1605 1146 498 255 225 163 111 107 54 10 XmnI GAAnn'nnTTC Cuts at: 6848 6848 Size: 8719 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5310 6527 276 Size: 53 83 186 936 3776 1217 2468 Fragments arranged by size: 3776 2468 1217 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV NruI NsiI PacI PmlI PsrI RsrII SwaI