Restriction map for
pFN21AE2308
(Linear) (Six-base) MAPSORT of: KIEE2308.seq Check: 6221 from: 1 to: 4014
KIEE2308.orf
With 182 enzymes: SgfI *
December 5, 2014 12:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 585 2452 3327 4014
Size: 585 1867 875 687
Fragments arranged by size:
1867 875 687 585
AatII G_ACGT'C
Cuts at: 0 3307 4014
Size: 3307 707
Acc65I G'GTAC_C
Cuts at: 0 404 4014
Size: 404 3610
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 2931 4014
Size: 625 2306 1083
Fragments arranged by size:
2306 1083 625
AfeI AGC'GCT
Cuts at: 0 136 3885 4014
Size: 136 3749 129
Fragments arranged by size:
3749 136 129
AflIII A'CryG_T
Cuts at: 0 2303 4014
Size: 2303 1711
AhdI GACnn_n'nnGTC
Cuts at: 0 3665 3975 4014
Size: 3665 310 39
Fragments arranged by size:
3665 310 39
AleI CACnn'nnGTG
Cuts at: 0 3478 4003 4014
Size: 3478 525 11
Fragments arranged by size:
3478 525 11
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2296 4014
Size: 2296 1718
AlwNI CAG_nnn'CTG
Cuts at: 0 1698 3283 4014
Size: 1698 1585 731
Fragments arranged by size:
1698 1585 731
ApaI G_GGCC'C
Cuts at: 0 2540 2787 3166 4014
Size: 2540 247 379 848
Fragments arranged by size:
2540 848 379 247
AscI GG'CGCG_CC
Cuts at: 0 260 4014
Size: 260 3754
AvaI C'yCGr_G
Cuts at: 0 28 217 1174 1365 1464 1561 1589 1677
Size: 28 189 957 191 99 97 28 88
Cuts at: 1677 1815 2209 2779 2886 2979 3022 3111 3165
Size: 138 394 570 107 93 43 89 54
Cuts at: 3165 3663 3673 3784 3868 4014
Size: 498 10 111 84 146
Fragments arranged by size:
957 570 498 394 191 189 146 138
111 107 99 97 93 89 88 84
54 43 28 28 10
BanI G'GyrC_C
Cuts at: 0 198 404 505 526 1135 1758 2332 2881
Size: 198 206 101 21 609 623 574 549
Cuts at: 2881 2890 2974 3169 3394 3627 3639 3934 4014
Size: 9 84 195 225 233 12 295 80
Fragments arranged by size:
623 609 574 549 295 233 225 206
198 195 101 84 80 21 12 9
BanII G_rGCy'C
Cuts at: 0 10 890 1165 1174 1372 1471 1927 2038
Size: 10 880 275 9 198 99 456 111
Cuts at: 2038 2206 2482 2540 2738 2746 2780 2787 2878
Size: 168 276 58 198 8 34 7 91
Cuts at: 2878 3001 3166 3847 4014
Size: 123 165 681 167
Fragments arranged by size:
880 681 456 276 275 198 198 168
167 165 123 111 99 91 58 34
10 9 8 7
BbeI G_GCGC'C
Cuts at: 0 202 509 530 1139 2978 3173 3398 3631
Size: 202 307 21 609 1839 195 225 233
Cuts at: 3631 4014
Size: 383
Fragments arranged by size:
1839 609 383 307 233 225 202 195
21
BbvCI CC'TCA_GC
Cuts at: 0 3827 4014
Size: 3827 187
BciVI GTATCCnnnnn_n'
Cuts at: 0 3050 4014
Size: 3050 964
BglI GCCn_nnn'nGGC
Cuts at: 0 30 535 743 874 1007 1655 1746 2124
Size: 30 505 208 131 133 648 91 378
Cuts at: 2124 2563 2610 2633 2651 3292 4014
Size: 439 47 23 18 641 722
Fragments arranged by size:
722 648 641 505 439 378 208 133
131 91 47 30 23 18
BglII A'GATC_T
Cuts at: 0 2063 4014
Size: 2063 1951
BlpI GC'TnA_GC
Cuts at: 0 3322 4014
Size: 3322 692
Bme1580I G_kGCm'C
Cuts at: 0 1763 2335 2540 2787 2893 3166 3939 4014
Size: 1763 572 205 247 106 273 773 75
Fragments arranged by size:
1763 773 572 273 247 205 106 75
BmgBI CAC'GTC
Cuts at: 0 3771 4014
Size: 3771 243
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1527 1929 3048 3471 4014
Size: 1527 402 1119 423 543
Fragments arranged by size:
1527 1119 543 423 402
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 883 1428 1497 1911 2521 2536 2704 3602
Size: 883 545 69 414 610 15 168 898
Cuts at: 3602 4014
Size: 412
Fragments arranged by size:
898 883 610 545 414 412 168 69
15
Bpu10I CC'TnA_GC
Cuts at: 0 1168 1195 1213 1645 2383 2877 2966 3827
Size: 1168 27 18 432 738 494 89 861
Cuts at: 3827 4014
Size: 187
Fragments arranged by size:
1168 861 738 494 432 187 89 27
18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 4014
Size: 865 3149
BsaI GGTCTCn'nnnn_
Cuts at: 0 847 1680 2921 3442 3718 3754 3880 4014
Size: 847 833 1241 521 276 36 126 134
Fragments arranged by size:
1241 847 833 521 276 134 126 36
BsaAI yAC'GTr
Cuts at: 0 2469 4014
Size: 2469 1545
BsaHI Gr'CG_yC
Cuts at: 0 199 317 506 527 1136 1579 1969 2818
Size: 199 118 189 21 609 443 390 849
Cuts at: 2818 2975 3170 3304 3395 3628 3893 4014
Size: 157 195 134 91 233 265 121
Fragments arranged by size:
849 609 443 390 265 233 199 195
189 157 134 121 118 91 21
BsaWI w'CCGG_w
Cuts at: 0 2730 4014
Size: 2730 1284
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 388 418 1270 1300 1909 1939 2335 2365
Size: 388 30 852 30 609 30 396 30
Cuts at: 2365 2935 2965 3325 3355 3508 3538 3829 3859
Size: 570 30 360 30 153 30 291 30
Cuts at: 3859 4014
Size: 155
Fragments arranged by size:
852 609 570 396 388 360 291 155
153 30 30 30 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1761 2518 2521 2826 3021 3447 4014
Size: 1761 757 3 305 195 426 567
Fragments arranged by size:
1761 757 567 426 305 195 3
BseYI C'CCAG_C
Cuts at: 0 997 1156 1474 1763 2311 2539 2695 2704
Size: 997 159 318 289 548 228 156 9
Cuts at: 2704 2871 3797 4014
Size: 167 926 217
Fragments arranged by size:
997 926 548 318 289 228 217 167
159 156 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1086 1402 2347 4014
Size: 1086 316 945 1667
Fragments arranged by size:
1667 1086 945 316
BsiEI CG_ry'CG
Cuts at: 0 446 575 788 904 1130 1233 1756 2625
Size: 446 129 213 116 226 103 523 869
Cuts at: 2625 2655 3146 3655 4014
Size: 30 491 509 359
Fragments arranged by size:
869 523 509 491 446 359 226 213
129 116 103 30
BsiHKAI G_wGCw'C
Cuts at: 0 890 1927 2206 2474 2482 3847 4014
Size: 890 1037 279 268 8 1365 167
Fragments arranged by size:
1365 1037 890 279 268 167 8
BsmBI CGTCTCn'nnnn_
Cuts at: 0 38 446 1570 1586 3086 4014
Size: 38 408 1124 16 1500 928
Fragments arranged by size:
1500 1124 928 408 38 16
Bsp1286I G_dGCh'C
Cuts at: 0 10 890 1165 1174 1372 1471 1763 1927
Size: 10 880 275 9 198 99 292 164
Cuts at: 1927 2038 2206 2335 2474 2482 2540 2738 2746
Size: 111 168 129 139 8 58 198 8
Cuts at: 2746 2780 2787 2878 2893 3001 3166 3847 3939
Size: 34 7 91 15 108 165 681 92
Cuts at: 3939 4014
Size: 75
Fragments arranged by size:
880 681 292 275 198 198 168 165
164 139 129 111 108 99 92 91
75 58 34 15 10 9 8 8
7
BspHI T'CATG_A
Cuts at: 0 725 2474 4014
Size: 725 1749 1540
Fragments arranged by size:
1749 1540 725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 116 585 998 1535 2452 3327 4014
Size: 116 469 413 537 917 875 687
Fragments arranged by size:
917 875 687 537 469 413 116
BsrBI CCG'CTC
Cuts at: 0 41 782 1022 1123 1202 1217 1286 1661
Size: 41 741 240 101 79 15 69 375
Cuts at: 1661 2000 2382 2423 2922 2984 3701 4014
Size: 339 382 41 499 62 717 313
Fragments arranged by size:
741 717 499 382 375 339 313 240
101 79 69 62 41 41 15
BsrFI r'CCGG_y
Cuts at: 0 33 523 1053 1143 1179 1257 1755 2566
Size: 33 490 530 90 36 78 498 811
Cuts at: 2566 2906 3105 3624 3650 3733 3904 4014
Size: 340 199 519 26 83 171 110
Fragments arranged by size:
811 530 519 498 490 340 199 171
110 90 83 78 36 33 26
BssHII G'CGCG_C
Cuts at: 0 260 309 410 667 962 964 1185 1187
Size: 260 49 101 257 295 2 221 2
Cuts at: 1187 1268 1530 4014
Size: 81 262 2484
Fragments arranged by size:
2484 295 262 260 257 221 101 81
49 2 2
BssSI C'ACGA_G
Cuts at: 0 850 1071 2713 4014
Size: 850 221 1642 1301
Fragments arranged by size:
1642 1301 850 221
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1073 1416 2067 2237 4014
Size: 1073 343 651 170 1777
Fragments arranged by size:
1777 1073 651 343 170
BstEII G'GTnAC_C
Cuts at: 0 2322 3471 4014
Size: 2322 1149 543
Fragments arranged by size:
2322 1149 543
BstXI CCAn_nnnn'nTGG
Cuts at: 0 618 1302 4014
Size: 618 684 2712
Fragments arranged by size:
2712 684 618
BstYI r'GATC_y
Cuts at: 0 2063 4014
Size: 2063 1951
Bsu36I CC'TnA_GG
Cuts at: 0 2522 4014
Size: 2522 1492
BtgI C'CryG_G
Cuts at: 0 13 211 368 569 1130 1233 1301 1359
Size: 13 198 157 201 561 103 68 58
Cuts at: 1359 1422 1631 2255 2327 2486 2993 3390 3527
Size: 63 209 624 72 159 507 397 137
Cuts at: 3527 3655 4014
Size: 128 359
Fragments arranged by size:
624 561 507 397 359 209 201 198
159 157 137 128 103 72 68 63
58 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1554 3744 4014
Size: 1554 2190 270
Fragments arranged by size:
2190 1554 270
BtsI GCAGTG_nn'
Cuts at: 0 1998 2722 4014
Size: 1998 724 1292
Fragments arranged by size:
1998 1292 724
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3599 3634 4014
Size: 3599 35 380
Fragments arranged by size:
3599 380 35
DraIII CAC_nnn'GTG
Cuts at: 0 90 2994 4014
Size: 90 2904 1020
Fragments arranged by size:
2904 1020 90
EaeI y'GGCC_r
Cuts at: 0 126 371 443 572 744 785 901 935
Size: 126 245 72 129 172 41 116 34
Cuts at: 935 1040 1058 1127 1230 1356 1431 1656 1753
Size: 105 18 69 103 126 75 225 97
Cuts at: 1753 2549 2622 2652 2985 3143 3236 3293 3652
Size: 796 73 30 333 158 93 57 359
Cuts at: 3652 4014
Size: 362
Fragments arranged by size:
796 362 359 333 245 225 172 158
129 126 126 116 105 103 97 93
75 73 72 69 57 41 34 30
18
EagI C'GGCC_G
Cuts at: 0 443 572 785 901 1127 1230 1753 2622
Size: 443 129 213 116 226 103 523 869
Cuts at: 2622 2652 3143 3652 4014
Size: 30 491 509 362
Fragments arranged by size:
869 523 509 491 443 362 226 213
129 116 103 30
EarI CTCTTCn'nnn_
Cuts at: 0 895 3310 4014
Size: 895 2415 704
Fragments arranged by size:
2415 895 704
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 261 724 835 1449 2040 2838 3032 4014
Size: 261 463 111 614 591 798 194 982
Fragments arranged by size:
982 798 614 591 463 261 194 111
EcoICRI GAG'CTC
Cuts at: 0 888 1925 2204 2480 3845 4014
Size: 888 1037 279 276 1365 169
Fragments arranged by size:
1365 1037 888 279 276 169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 883 1428 1497 1911 2521 2536 2704
Size: 625 258 545 69 414 610 15 168
Cuts at: 2704 2931 3602 4014
Size: 227 671 412
Fragments arranged by size:
671 625 610 545 414 412 258 227
168 69 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2503 4014
Size: 2503 1511
EcoO109I rG'GnC_Cy
Cuts at: 0 796 1640 1695 1721 2116 2433 2536 2783
Size: 796 844 55 26 395 317 103 247
Cuts at: 2783 3162 3928 4014
Size: 379 766 86
Fragments arranged by size:
844 796 766 395 379 317 247 103
86 55 26
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 156 906 2082 4014
Size: 156 750 1176 1932
Fragments arranged by size:
1932 1176 750 156
FseI GG_CCGG'CC
Cuts at: 0 2570 3654 4014
Size: 2570 1084 360
Fragments arranged by size:
2570 1084 360
FspI TGC'GCA
Cuts at: 0 94 112 1084 1714 2230 3718 4014
Size: 94 18 972 630 516 1488 296
Fragments arranged by size:
1488 972 630 516 296 94 18
FspAI rTGC'GCAy
Cuts at: 0 1084 2230 4014
Size: 1084 1146 1784
Fragments arranged by size:
1784 1146 1084
HaeII r_GCGC'y
Cuts at: 0 138 202 494 509 530 880 1139 1252
Size: 138 64 292 15 21 350 259 113
Cuts at: 1252 1457 2558 2978 3173 3398 3513 3631 3887
Size: 205 1101 420 195 225 115 118 256
Cuts at: 3887 4014
Size: 127
Fragments arranged by size:
1101 420 350 292 259 256 225 205
195 138 127 118 115 113 64 21
15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 691 723 2335 2367 2935 2967 3325 3357
Size: 691 32 1612 32 568 32 358 32
Cuts at: 3357 3651 3683 3829 3861 3961 3993 4014
Size: 294 32 146 32 100 32 21
Fragments arranged by size:
1612 691 568 358 294 146 100 32
32 32 32 32 32 32 21
HincII GTy'rAC
Cuts at: 0 987 4014
Size: 987 3027
KasI G'GCGC_C
Cuts at: 0 198 505 526 1135 2974 3169 3394 3627
Size: 198 307 21 609 1839 195 225 233
Cuts at: 3627 4014
Size: 387
Fragments arranged by size:
1839 609 387 307 233 225 198 195
21
KpnI G_GTAC'C
Cuts at: 0 408 4014
Size: 408 3606
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2951 4014
Size: 2951 1063
MscI TGG'CCA
Cuts at: 0 128 3295 4014
Size: 128 3167 719
Fragments arranged by size:
3167 719 128
MslI CAynn'nnrTG
Cuts at: 0 772 1294 1300 1630 1798 1963 2029 2230
Size: 772 522 6 330 168 165 66 201
Cuts at: 2230 2267 3220 3478 3769 4003 4014
Size: 37 953 258 291 234 11
Fragments arranged by size:
953 772 522 330 291 258 234 201
168 165 66 37 11 6
MspA1I CmG'CkG
Cuts at: 0 15 213 740 1132 1235 1355 1416 1437
Size: 15 198 527 392 103 120 61 21
Cuts at: 1437 1448 1752 2006 2913 3392 4014
Size: 11 304 254 907 479 622
Fragments arranged by size:
907 622 527 479 392 304 254 198
120 103 61 21 15 11
NaeI GCC'GGC
Cuts at: 0 35 525 1055 1259 2568 2908 3107 3626
Size: 35 490 530 204 1309 340 199 519
Cuts at: 3626 3652 3735 4014
Size: 26 83 279
Fragments arranged by size:
1309 530 519 490 340 279 204 199
83 35 26
NarI GG'CG_CC
Cuts at: 0 199 506 527 1136 2975 3170 3395 3628
Size: 199 307 21 609 1839 195 225 233
Cuts at: 3628 4014
Size: 386
Fragments arranged by size:
1839 609 386 307 233 225 199 195
21
NcoI C'CATG_G
Cuts at: 0 1631 2255 4014
Size: 1631 624 1759
Fragments arranged by size:
1759 1631 624
NgoMIV G'CCGG_C
Cuts at: 0 33 523 1053 1257 2566 2906 3105 3624
Size: 33 490 530 204 1309 340 199 519
Cuts at: 3624 3650 3733 4014
Size: 26 83 281
Fragments arranged by size:
1309 530 519 490 340 281 204 199
83 33 26
NotI GC'GGCC_GC
Cuts at: 0 443 1127 1230 2622 4014
Size: 443 684 103 1392 1392
Fragments arranged by size:
1392 1392 684 443 103
NspI r_CATG'y
Cuts at: 0 1962 2028 2229 2235 2266 2712 4014
Size: 1962 66 201 6 31 446 1302
Fragments arranged by size:
1962 1302 446 201 66 31 6
PasI CC'CwG_GG
Cuts at: 0 510 2075 3159 4014
Size: 510 1565 1084 855
Fragments arranged by size:
1565 1084 855 510
PflMI CCAn_nnn'nTGG
Cuts at: 0 981 1698 2994 4014
Size: 981 717 1296 1020
Fragments arranged by size:
1296 1020 981 717
PfoI T'CCnGG_A
Cuts at: 0 860 2498 4014
Size: 860 1638 1516
Fragments arranged by size:
1638 1516 860
PpuMI rG'GwC_Cy
Cuts at: 0 1695 1721 3928 4014
Size: 1695 26 2207 86
Fragments arranged by size:
2207 1695 86 26
PspOMI G'GGCC_C
Cuts at: 0 2536 2783 3162 4014
Size: 2536 247 379 852
Fragments arranged by size:
2536 852 379 247
PspXI vC'TCGA_Gb
Cuts at: 0 217 4014
Size: 217 3797
PstI C_TGCA'G
Cuts at: 0 490 722 1543 2011 3089 3365 4014
Size: 490 232 821 468 1078 276 649
Fragments arranged by size:
1078 821 649 490 468 276 232
PvuII CAG'CTG
Cuts at: 0 2006 4014
Size: 2006 2008
SacI G_AGCT'C
Cuts at: 0 890 1927 2206 2482 3847 4014
Size: 890 1037 279 276 1365 167
Fragments arranged by size:
1365 1037 890 279 276 167
SacII CC_GC'GG
Cuts at: 0 16 214 1133 1236 3393 4014
Size: 16 198 919 103 2157 621
Fragments arranged by size:
2157 919 621 198 103 16
SanDI GG'GwC_CC
Cuts at: 0 3928 4014
Size: 3928 86
SapI GCTCTTCn'nnn_
Cuts at: 0 895 4014
Size: 895 3119
SbfI CC_TGCA'GG
Cuts at: 0 3365 4014
Size: 3365 649
ScaI AGT'ACT
Cuts at: 0 223 361 4014
Size: 223 138 3653
Fragments arranged by size:
3653 223 138
SexAI A'CCwGG_T
Cuts at: 0 422 980 4014
Size: 422 558 3034
Fragments arranged by size:
3034 558 422
SfcI C'TryA_G
Cuts at: 0 486 718 1539 2007 2406 2591 3085 3361
Size: 486 232 821 468 399 185 494 276
Cuts at: 3361 4014
Size: 653
Fragments arranged by size:
821 653 494 486 468 399 276 232
185
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 30 2124 2651 4014
Size: 30 2094 527 1363
Fragments arranged by size:
2094 1363 527 30
SfoI GGC'GCC
Cuts at: 0 200 507 528 1137 2976 3171 3396 3629
Size: 200 307 21 609 1839 195 225 233
Cuts at: 3629 4014
Size: 385
Fragments arranged by size:
1839 609 385 307 233 225 200 195
21
SgrAI Cr'CCGG_yG
Cuts at: 0 1053 3904 4014
Size: 1053 2851 110
Fragments arranged by size:
2851 1053 110
SmaI CCC'GGG
Cuts at: 0 30 1176 2781 2888 3113 3167 3665 3675
Size: 30 1146 1605 107 225 54 498 10
Cuts at: 3675 3786 4014
Size: 111 228
Fragments arranged by size:
1605 1146 498 228 225 111 107 54
30 10
SmlI C'TyrA_G
Cuts at: 0 217 880 4014
Size: 217 663 3134
Fragments arranged by size:
3134 663 217
SphI G_CATG'C
Cuts at: 0 2266 2712 4014
Size: 2266 446 1302
Fragments arranged by size:
2266 1302 446
SrfI GCCC'GGGC
Cuts at: 0 30 3167 4014
Size: 30 3137 847
Fragments arranged by size:
3137 847 30
StuI AGG'CCT
Cuts at: 0 3028 3355 4014
Size: 3028 327 659
Fragments arranged by size:
3028 659 327
StyI C'CwwG_G
Cuts at: 0 350 611 1631 1862 2255 3002 3398 4014
Size: 350 261 1020 231 393 747 396 616
Fragments arranged by size:
1020 747 616 396 393 350 261 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1718 2148 3559 4014
Size: 1718 430 1411 455
Fragments arranged by size:
1718 1411 455 430
TatI w'GTAC_w
Cuts at: 0 221 359 2753 4014
Size: 221 138 2394 1261
Fragments arranged by size:
2394 1261 221 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1270 1302 1909 1941 4014
Size: 1270 32 607 32 2073
Fragments arranged by size:
2073 1270 607 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 424 2320 4014
Size: 424 1896 1694
Fragments arranged by size:
1896 1694 424
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1935 2253 4014
Size: 1935 318 1761
Fragments arranged by size:
1935 1761 318
XhoI C'TCGA_G
Cuts at: 0 217 4014
Size: 217 3797
XmaI C'CCGG_G
Cuts at: 0 28 1174 2779 2886 3111 3165 3663 3673
Size: 28 1146 1605 107 225 54 498 10
Cuts at: 3673 3784 4014
Size: 111 230
Fragments arranged by size:
1605 1146 498 230 225 111 107 54
28 10
ZraI GAC'GTC
Cuts at: 0 3305 4014
Size: 3305 709
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaI AscI AvaI BanI BanII BbeI
BbvCI BciVI BglI BglII BlpI Bme1580I BmgBI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FseI FspI FspAI HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NspI PasI PflMI PfoI PpuMI PspOMI PspXI
PstI PvuII SacI SacII SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AccI AclI AflII AgeI AloI ApaLI ApoI AseI
AsiSI AvrII BaeI BamHI BbsI BcgI BclI BfrBI
BmrI BmtI BsaBI BsiWI BsmI BspEI BsrDI BsrGI
BstBI BstZ17I ClaI DraI DrdI EcoRI EcoRV HindIII
HpaI MfeI MluI NdeI NheI NruI NsiI PacI
PciI PmeI PmlI PpiI PshAI PsiI PsrI PvuI
RsrII SalI SgfI SnaBI SpeI SspI SwaI XbaI
XmnI