(Circular) (Six-base) MAPSORT of: pFN21AE9612.seq Check: 595 from: 1 to: 5641 pFN21AE9612 5641 bp With 182 enzymes: SgfI * March 10, 2017 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3451 278 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2173 2984 1937 Size: 236 811 4594 Fragments arranged by size: 4594 811 236 Acc65I G'GTAC_C Cuts at: 2962 2962 Size: 5641 AclI AA'CG_TT Cuts at: 1563 3768 4141 1563 Size: 2205 373 3063 Fragments arranged by size: 3063 2205 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2700 3704 5088 5356 1632 Size: 1068 1004 1384 268 1917 Fragments arranged by size: 1917 1384 1068 1004 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5444 AflIII A'CryG_T Cuts at: 1203 2052 2364 4540 5517 1203 Size: 849 312 2176 977 1327 Fragments arranged by size: 2176 1327 977 849 312 AgeI A'CCGG_T Cuts at: 4472 4472 Size: 5641 AhdI GACnn_n'nnGTC Cuts at: 4370 4370 Size: 5641 AleI CACnn'nnGTG Cuts at: 1166 2273 1166 Size: 1107 4534 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2402 1184 Size: 1218 4423 AlwNI CAG_nnn'CTG Cuts at: 2182 3384 4956 2182 Size: 1202 1572 2867 Fragments arranged by size: 2867 1572 1202 ApaLI G'TGCA_C Cuts at: 3698 4854 3698 Size: 1156 4485 ApoI r'AATT_y Cuts at: 1493 2950 3176 3328 5437 1493 Size: 1457 226 152 2109 1697 Fragments arranged by size: 2109 1697 1457 226 152 AseI AT'TA_AT Cuts at: 160 1032 4195 160 Size: 872 3163 1606 Fragments arranged by size: 3163 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 AvaI C'yCGr_G Cuts at: 1870 1943 2565 2966 1870 Size: 73 622 401 4545 Fragments arranged by size: 4545 622 401 73 BamHI G'GATC_C Cuts at: 2278 2971 3338 2278 Size: 693 367 4581 Fragments arranged by size: 4581 693 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 2962 4417 618 Size: 325 217 627 252 923 1455 1842 Fragments arranged by size: 1842 1455 923 627 325 252 217 BanII G_rGCy'C Cuts at: 1391 2960 1391 Size: 1569 4072 BbeI G_GCGC'C Cuts at: 2043 2043 Size: 5641 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2246 2495 2822 928 Size: 971 347 249 327 3747 Fragments arranged by size: 3747 971 347 327 249 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3832 3866 1903 Size: 34 1895 34 3678 Fragments arranged by size: 3678 1895 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3533 4743 3533 Size: 1210 4431 BclI T'GATC_A Cuts at: 1548 2344 1548 Size: 796 4845 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4252 136 Size: 107 122 71 631 605 2580 1525 Fragments arranged by size: 2580 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5636 5636 Size: 5641 BlpI GC'TnA_GC Cuts at: 3058 3058 Size: 5641 Bme1580I G_kGCm'C Cuts at: 1790 3702 4858 1790 Size: 1912 1156 2573 Fragments arranged by size: 2573 1912 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5641 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3372 4330 442 Size: 416 53 83 399 24 1955 958 1753 Fragments arranged by size: 1955 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5641 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2273 2273 Size: 5641 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4301 1239 Size: 3062 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2426 3766 4631 4929 2426 Size: 1340 865 298 3138 Fragments arranged by size: 3138 1340 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4304 882 Size: 617 2805 2219 Fragments arranged by size: 2805 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2053 3432 493 Size: 711 849 1379 2702 Fragments arranged by size: 2702 1379 849 711 BsaBI GATnn'nnATC Cuts at: 5635 5635 Size: 5641 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2040 3448 3830 4465 Size: 53 83 186 936 507 1408 382 635 Cuts at: 4465 275 Size: 1451 Fragments arranged by size: 1451 1408 936 635 507 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4074 4472 4746 4893 5304 4074 Size: 398 274 147 411 4411 Fragments arranged by size: 4411 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2315 2345 1178 Size: 30 165 30 912 30 4474 Fragments arranged by size: 4474 912 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5641 BseYI C'CCAG_C Cuts at: 4844 5340 4844 Size: 496 5145 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5068 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3091 3852 4880 1540 Size: 275 186 1090 761 1028 2301 Fragments arranged by size: 2301 1090 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3702 3787 4858 3702 Size: 85 1071 4485 Fragments arranged by size: 4485 1071 85 BsmI GAATG_Cn' Cuts at: 3152 3245 3152 Size: 93 5548 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2960 3702 3787 4858 1391 Size: 399 1170 742 85 1071 2174 Fragments arranged by size: 2174 1170 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5304 5304 Size: 5641 BspHI T'CATG_A Cuts at: 3530 5260 3530 Size: 1730 3911 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2996 844 Size: 2152 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3528 1394 Size: 429 1705 3507 Fragments arranged by size: 3507 1705 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2723 4136 4310 65 Size: 1183 1475 1413 174 1396 Fragments arranged by size: 1475 1413 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4285 4472 1700 Size: 110 2475 187 2869 Fragments arranged by size: 2869 2475 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5641 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5641 BssSI C'ACGA_G Cuts at: 3701 4713 3701 Size: 1012 4629 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5641 BstBI TT'CG_AA Cuts at: 4445 4445 Size: 5641 BstEII G'GTnAC_C Cuts at: 1184 2594 1184 Size: 1410 4231 BstXI CCAn_nnnn'nTGG Cuts at: 2275 2275 Size: 5641 BstYI r'GATC_y Cuts at: 1972 2278 2971 3338 3723 3740 5192 5278 5290 Size: 306 693 367 385 17 1452 86 12 Cuts at: 5290 5636 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 693 385 367 346 306 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2641 513 Size: 552 939 637 3513 Fragments arranged by size: 3513 939 637 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4902 Fragments arranged by size: 4902 683 56 BtsI GCAGTG_nn' Cuts at: 3157 3951 3971 3157 Size: 794 20 4827 Fragments arranged by size: 4827 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5641 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5606 DraI TTT'AAA Cuts at: 2076 2945 2076 Size: 869 4772 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4648 809 Size: 507 3332 1802 Fragments arranged by size: 3332 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3088 3977 Size: 54 1425 50 275 18 177 1081 889 Cuts at: 3977 5606 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1081 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3088 1537 Size: 275 1276 4090 Fragments arranged by size: 4090 1276 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2613 3571 1353 Size: 1260 958 3423 Fragments arranged by size: 3423 1260 958 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4228 4602 4748 387 Size: 1064 138 2639 374 146 1280 Fragments arranged by size: 2639 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2700 3704 4301 5088 5356 1239 Size: 393 1068 1004 597 787 268 1524 Fragments arranged by size: 1524 1068 1004 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2309 2309 Size: 5641 EcoRI G'AATT_C Cuts at: 2950 3328 2950 Size: 378 5263 EcoRV GAT'ATC Cuts at: 2652 2652 Size: 5641 FspI TGC'GCA Cuts at: 4147 4147 Size: 5641 HaeII r_GCGC'y Cuts at: 1678 2043 4788 1678 Size: 365 2745 2531 Fragments arranged by size: 2745 2531 365 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2672 2704 2833 2865 4282 Size: 32 135 32 1100 32 129 32 1417 Cuts at: 4282 4314 4356 4388 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1417 1100 135 129 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2128 2577 2872 2985 3231 669 Size: 988 281 190 449 295 113 246 3079 Fragments arranged by size: 3079 988 449 295 281 246 190 113 HindIII A'AGCT_T Cuts at: 748 2601 2702 748 Size: 1853 101 3687 Fragments arranged by size: 3687 1853 101 HpaI GTT'AAC Cuts at: 2128 3231 2128 Size: 1103 4538 KasI G'GCGC_C Cuts at: 2039 2039 Size: 5641 KpnI G_GTAC'C Cuts at: 2966 2966 Size: 5641 MfeI C'AATT_G Cuts at: 3240 3240 Size: 5641 MluI A'CGCG_T Cuts at: 5517 5517 Size: 5641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4755 4939 1305 Size: 3450 184 2007 Fragments arranged by size: 3450 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3819 Fragments arranged by size: 3819 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2273 2497 3599 3958 4117 518 Size: 648 1107 224 1102 359 159 2042 Fragments arranged by size: 2042 1107 1102 648 359 224 159 MspA1I CmG'CkG Cuts at: 1606 2012 2182 3057 3734 4882 5127 1606 Size: 406 170 875 677 1148 245 2120 Fragments arranged by size: 2120 1148 875 677 406 245 170 NarI GG'CG_CC Cuts at: 2040 2040 Size: 5641 NcoI C'CATG_G Cuts at: 513 1065 2004 2641 513 Size: 552 939 637 3513 Fragments arranged by size: 3513 939 637 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5641 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5641 NotI GC'GGCC_GC Cuts at: 3088 3088 Size: 5641 NspI r_CATG'y Cuts at: 1132 1229 2999 4544 1132 Size: 97 1770 1545 2229 Fragments arranged by size: 2229 1770 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5641 PciI A'CATG_T Cuts at: 4540 4540 Size: 5641 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5641 PfoI T'CCnGG_A Cuts at: 2398 2398 Size: 5641 PmeI GTTT'AAAC Cuts at: 2945 2945 Size: 5641 PmlI CAC'GTG Cuts at: 2053 2053 Size: 5641 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 5223 5255 3710 Size: 32 1481 32 4096 Fragments arranged by size: 4096 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5641 PsiI TTA'TAA Cuts at: 2228 3211 2228 Size: 983 4658 PstI C_TGCA'G Cuts at: 830 2848 2993 830 Size: 2018 145 3478 Fragments arranged by size: 3478 2018 145 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5641 PvuII CAG'CTG Cuts at: 2182 2182 Size: 5641 SalI G'TCGA_C Cuts at: 1936 2983 1936 Size: 1047 4594 SbfI CC_TGCA'GG Cuts at: 2993 2993 Size: 5641 ScaI AGT'ACT Cuts at: 3889 5601 3889 Size: 1712 3929 SfcI C'TryA_G Cuts at: 826 1045 2174 2769 2844 2989 4124 4805 4996 Size: 219 1129 595 75 145 1135 681 191 Cuts at: 4996 826 Size: 1471 Fragments arranged by size: 1471 1135 1129 681 595 219 191 145 75 SfoI GGC'GCC Cuts at: 2041 2041 Size: 5641 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5641 SmaI CCC'GGG Cuts at: 1872 2567 2968 1872 Size: 695 401 4545 Fragments arranged by size: 4545 695 401 SmlI C'TyrA_G Cuts at: 820 1017 1943 2405 3745 4646 4908 820 Size: 197 926 462 1340 901 262 1553 Fragments arranged by size: 1553 1340 926 901 462 262 197 SnaBI TAC'GTA Cuts at: 493 3432 493 Size: 2939 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5641 SphI G_CATG'C Cuts at: 1132 2999 1132 Size: 1867 3774 SspI AAT'ATT Cuts at: 5 52 2072 2468 3565 5 Size: 47 2020 396 1097 2081 Fragments arranged by size: 2081 2020 1097 396 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5641 StyI C'CwwG_G Cuts at: 513 1065 2004 2641 3080 513 Size: 552 939 637 439 3074 Fragments arranged by size: 3074 939 637 552 439 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3668 3685 3838 4023 4468 325 Size: 1255 2088 17 153 185 445 1498 Fragments arranged by size: 2088 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2475 3887 5599 Size: 274 80 33 51 1443 498 1412 1712 Cuts at: 5599 96 Size: 138 Fragments arranged by size: 1712 1443 1412 498 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5601 5633 5601 Size: 32 5609 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5641 XbaI T'CTAG_A Cuts at: 2977 2977 Size: 5641 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5641 XmaI C'CCGG_G Cuts at: 1870 2565 2966 1870 Size: 695 401 4545 Fragments arranged by size: 4545 695 401 XmnI GAAnn'nnTTC Cuts at: 3770 3770 Size: 5641 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3449 276 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI