Restriction map for
pFN21AE9612
(Linear) (Six-base) MAPSORT of: KIEE9612.seq Check: 3201 from: 1 to: 936
KIEE9612.orf
With 182 enzymes: SgfI *
March 10, 2017 10:38 ..
AccI GT'mk_AC
Cuts at: 0 168 936
Size: 168 768
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 936
Size: 695 241
AflIII A'CryG_T
Cuts at: 0 47 359 936
Size: 47 312 577
Fragments arranged by size:
577 312 47
AleI CACnn'nnGTG
Cuts at: 0 268 936
Size: 268 668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 397 936
Size: 397 539
AlwNI CAG_nnn'CTG
Cuts at: 0 177 936
Size: 177 759
AvaI C'yCGr_G
Cuts at: 0 560 936
Size: 560 376
BamHI G'GATC_C
Cuts at: 0 273 936
Size: 273 663
BanI G'GyrC_C
Cuts at: 0 34 936
Size: 34 902
BbeI G_GCGC'C
Cuts at: 0 38 936
Size: 38 898
BbsI GAAGACnn'nnnn_
Cuts at: 0 241 490 817 936
Size: 241 249 327 119
Fragments arranged by size:
327 249 241 119
BclI T'GATC_A
Cuts at: 0 339 936
Size: 339 597
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 268 936
Size: 268 668
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 936
Size: 421 515
BsaAI yAC'GTr
Cuts at: 0 48 936
Size: 48 888
BsaHI Gr'CG_yC
Cuts at: 0 35 936
Size: 35 901
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 310 340 936
Size: 310 30 596
Fragments arranged by size:
596 310 30
BsrDI GCAATG_nn'
Cuts at: 0 718 936
Size: 718 218
BstEII G'GTnAC_C
Cuts at: 0 589 936
Size: 589 347
BstXI CCAn_nnnn'nTGG
Cuts at: 0 270 936
Size: 270 666
BstYI r'GATC_y
Cuts at: 0 273 936
Size: 273 663
BtgI C'CryG_G
Cuts at: 0 636 936
Size: 636 300
DraI TTT'AAA
Cuts at: 0 71 936
Size: 71 865
EaeI y'GGCC_r
Cuts at: 0 2 936
Size: 2 934
EarI CTCTTCn'nnn_
Cuts at: 0 608 936
Size: 608 328
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 936
Size: 695 241
EcoNI CCTnn'n_nnAGG
Cuts at: 0 304 936
Size: 304 632
EcoRV GAT'ATC
Cuts at: 0 647 936
Size: 647 289
HaeII r_GCGC'y
Cuts at: 0 38 936
Size: 38 898
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 667 699 828 860 936
Size: 667 32 129 32 76
Fragments arranged by size:
667 129 76 32 32
HincII GTy'rAC
Cuts at: 0 123 572 867 936
Size: 123 449 295 69
Fragments arranged by size:
449 295 123 69
HindIII A'AGCT_T
Cuts at: 0 596 697 936
Size: 596 101 239
Fragments arranged by size:
596 239 101
HpaI GTT'AAC
Cuts at: 0 123 936
Size: 123 813
KasI G'GCGC_C
Cuts at: 0 34 936
Size: 34 902
MslI CAynn'nnrTG
Cuts at: 0 268 492 936
Size: 268 224 444
Fragments arranged by size:
444 268 224
MspA1I CmG'CkG
Cuts at: 0 7 177 936
Size: 7 170 759
Fragments arranged by size:
759 170 7
NarI GG'CG_CC
Cuts at: 0 35 936
Size: 35 901
NcoI C'CATG_G
Cuts at: 0 636 936
Size: 636 300
PfoI T'CCnGG_A
Cuts at: 0 393 936
Size: 393 543
PmlI CAC'GTG
Cuts at: 0 48 936
Size: 48 888
PsiI TTA'TAA
Cuts at: 0 223 936
Size: 223 713
PstI C_TGCA'G
Cuts at: 0 843 936
Size: 843 93
PvuII CAG'CTG
Cuts at: 0 177 936
Size: 177 759
SfcI C'TryA_G
Cuts at: 0 169 764 839 936
Size: 169 595 75 97
Fragments arranged by size:
595 169 97 75
SfoI GGC'GCC
Cuts at: 0 36 936
Size: 36 900
SmaI CCC'GGG
Cuts at: 0 562 936
Size: 562 374
SmlI C'TyrA_G
Cuts at: 0 400 936
Size: 400 536
SspI AAT'ATT
Cuts at: 0 67 463 936
Size: 67 396 473
Fragments arranged by size:
473 396 67
StyI C'CwwG_G
Cuts at: 0 636 936
Size: 636 300
TatI w'GTAC_w
Cuts at: 0 470 936
Size: 470 466
XmaI C'CCGG_G
Cuts at: 0 560 936
Size: 560 376
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI AvaI BamHI
BanI BbeI BbsI BclI BplI BpuEI BsaAI BsaHI
BsaXI BsrDI BstEII BstXI BstYI BtgI DraI EaeI
EarI Eco57MI EcoNI EcoRV HaeII Hin4I HincII HindIII
HpaI KasI MslI MspA1I NarI NcoI PfoI PmlI
PsiI PstI PvuII SfcI SfoI SmaI SmlI SspI
StyI TatI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BanII BbvCI BcgI BciVI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BsaI
BsaBI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraIII DrdI EagI EciI EcoICRI
EcoO109I EcoRI FalI FseI FspI FspAI KpnI MfeI
MluI MmeI MscI NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI StuI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmnI
ZraI