(Circular) (Six-base) MAPSORT of: pFN21AE1764.seq Check: 2069 from: 1 to: 5515 pFN21AE1764 5515 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3325 278 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2858 1937 Size: 921 4594 Acc65I G'GTAC_C Cuts at: 2836 2836 Size: 5515 AclI AA'CG_TT Cuts at: 1563 3642 4015 1563 Size: 2079 373 3063 Fragments arranged by size: 3063 2079 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3578 4962 5230 1632 Size: 1946 1384 268 1917 Fragments arranged by size: 1946 1917 1384 268 AfeI AGC'GCT Cuts at: 2600 2600 Size: 5515 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5318 AflIII A'CryG_T Cuts at: 1203 4414 5391 1203 Size: 3211 977 1327 Fragments arranged by size: 3211 1327 977 AgeI A'CCGG_T Cuts at: 2769 4346 2769 Size: 1577 3938 AhdI GACnn_n'nnGTC Cuts at: 4244 4244 Size: 5515 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5515 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5515 AlwNI CAG_nnn'CTG Cuts at: 3258 4830 3258 Size: 1572 3943 ApaLI G'TGCA_C Cuts at: 3572 4728 3572 Size: 1156 4359 ApoI r'AATT_y Cuts at: 1493 2180 2824 3050 3202 5311 1493 Size: 687 644 226 152 2109 1697 Fragments arranged by size: 2109 1697 687 644 226 152 AseI AT'TA_AT Cuts at: 160 1032 4069 160 Size: 872 3037 1606 Fragments arranged by size: 3037 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 AvaI C'yCGr_G Cuts at: 1870 1943 2077 2840 1870 Size: 73 134 763 4545 Fragments arranged by size: 4545 763 134 73 BamHI G'GATC_C Cuts at: 2845 3212 2845 Size: 367 5148 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2084 2164 2608 2836 4291 Size: 325 217 627 297 80 444 228 1455 Cuts at: 4291 618 Size: 1842 Fragments arranged by size: 1842 1455 627 444 325 297 228 217 80 BanII G_rGCy'C Cuts at: 1391 2424 2577 2697 2834 1391 Size: 1033 153 120 137 4072 Fragments arranged by size: 4072 1033 153 137 120 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2136 928 Size: 971 237 4307 Fragments arranged by size: 4307 971 237 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3706 3740 1903 Size: 34 1769 34 3678 Fragments arranged by size: 3678 1769 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3407 4617 3407 Size: 1210 4305 BclI T'GATC_A Cuts at: 1548 1548 Size: 5515 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2442 4126 136 Size: 107 122 71 631 605 770 1684 1525 Fragments arranged by size: 1684 1525 770 631 605 122 107 71 BglII A'GATC_T Cuts at: 5510 5510 Size: 5515 BlpI GC'TnA_GC Cuts at: 2756 2932 2756 Size: 176 5339 Bme1580I G_kGCm'C Cuts at: 1790 2087 2611 3576 4732 1790 Size: 297 524 965 1156 2573 Fragments arranged by size: 2573 1156 965 524 297 BmgBI CAC'GTC Cuts at: 1318 2704 1318 Size: 1386 4129 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3246 4204 442 Size: 416 53 83 399 24 1829 958 1753 Fragments arranged by size: 1829 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5515 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 2323 2621 4175 1239 Size: 818 266 298 1554 2579 Fragments arranged by size: 2579 1554 818 298 266 Bpu10I CC'TnA_GC Cuts at: 2691 2691 Size: 5515 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3640 4505 4803 3640 Size: 865 298 4352 Fragments arranged by size: 4352 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4178 882 Size: 617 2679 2219 Fragments arranged by size: 2679 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3306 493 Size: 711 2102 2702 Fragments arranged by size: 2702 2102 711 BsaBI GATnn'nnATC Cuts at: 5509 5509 Size: 5515 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2060 2133 3322 3704 Size: 53 83 186 936 527 73 1189 382 Cuts at: 3704 4339 275 Size: 635 1451 Fragments arranged by size: 1451 1189 936 635 527 382 186 83 73 53 BsaWI w'CCGG_w Cuts at: 2769 3948 4346 4620 4767 5178 2769 Size: 1179 398 274 147 411 3106 Fragments arranged by size: 3106 1179 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2619 2649 2736 2766 1178 Size: 30 165 30 1216 30 87 30 3927 Fragments arranged by size: 3927 1216 165 87 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2624 1186 Size: 1438 4077 BseYI C'CCAG_C Cuts at: 4718 5214 4718 Size: 496 5019 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4942 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2965 3726 4754 1540 Size: 275 186 964 761 1028 2301 Fragments arranged by size: 2301 1028 964 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2525 2757 3576 3661 4732 2525 Size: 232 819 85 1071 3308 Fragments arranged by size: 3308 1071 819 232 85 BsmI GAATG_Cn' Cuts at: 3026 3119 3026 Size: 93 5422 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2424 2525 2577 2611 2697 2757 Size: 399 297 337 101 52 34 86 60 Cuts at: 2757 2834 3576 3661 4732 1391 Size: 77 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 337 297 101 86 85 77 60 52 34 BspEI T'CCGG_A Cuts at: 5178 5178 Size: 5515 BspHI T'CATG_A Cuts at: 3404 5134 3404 Size: 1730 3785 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2870 844 Size: 2026 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2041 3402 1394 Size: 429 218 1361 3507 Fragments arranged by size: 3507 1361 429 218 BsrDI GCAATG_nn' Cuts at: 65 1248 4010 4184 65 Size: 1183 2762 174 1396 Fragments arranged by size: 2762 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2769 4159 4346 1700 Size: 110 959 1390 187 2869 Fragments arranged by size: 2869 1390 959 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5515 BssHII G'CGCG_C Cuts at: 1925 2026 1925 Size: 101 5414 BssSI C'ACGA_G Cuts at: 3575 4587 3575 Size: 1012 4503 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5515 BstBI TT'CG_AA Cuts at: 4319 4319 Size: 5515 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5515 BstYI r'GATC_y Cuts at: 1972 2109 2353 2845 3212 3597 3614 5066 5152 Size: 137 244 492 367 385 17 1452 86 Cuts at: 5152 5164 5510 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 492 385 367 346 244 137 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4024 Fragments arranged by size: 4024 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4776 Fragments arranged by size: 4776 683 56 BtsI GCAGTG_nn' Cuts at: 2026 3031 3825 3845 2026 Size: 1005 794 20 3696 Fragments arranged by size: 3696 1005 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5515 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5480 DraI TTT'AAA Cuts at: 2819 2819 Size: 5515 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4522 809 Size: 507 3206 1802 Fragments arranged by size: 3206 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2050 2237 2962 Size: 54 1425 50 275 18 220 187 725 Cuts at: 2962 3851 5480 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 725 275 220 187 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2962 1537 Size: 275 1150 4090 Fragments arranged by size: 4090 1150 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2147 2589 3445 1353 Size: 794 442 856 3423 Fragments arranged by size: 3423 856 794 442 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2280 4102 4476 4622 387 Size: 1064 138 691 1822 374 146 1280 Fragments arranged by size: 1822 1280 1064 691 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2323 2621 3578 4175 4962 5230 Size: 393 425 266 298 957 597 787 268 Cuts at: 5230 1239 Size: 1524 Fragments arranged by size: 1524 957 787 597 425 393 298 268 266 EcoNI CCTnn'n_nnAGG Cuts at: 2651 2651 Size: 5515 EcoO109I rG'GnC_Cy Cuts at: 2289 2558 2289 Size: 269 5246 EcoRI G'AATT_C Cuts at: 2180 2824 3202 2180 Size: 644 378 4493 Fragments arranged by size: 4493 644 378 FspI TGC'GCA Cuts at: 4021 4021 Size: 5515 HaeII r_GCGC'y Cuts at: 1678 2602 4662 1678 Size: 924 2060 2531 Fragments arranged by size: 2531 2060 924 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4156 4188 4230 4262 1373 Size: 32 135 32 2584 32 42 32 2626 Fragments arranged by size: 2626 2584 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2452 2859 3105 669 Size: 988 281 514 407 246 3079 Fragments arranged by size: 3079 988 514 407 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5515 HpaI GTT'AAC Cuts at: 2452 3105 2452 Size: 653 4862 KpnI G_GTAC'C Cuts at: 2840 2840 Size: 5515 MfeI C'AATT_G Cuts at: 3114 3114 Size: 5515 MluI A'CGCG_T Cuts at: 5391 5391 Size: 5515 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4629 4813 1305 Size: 3324 184 2007 Fragments arranged by size: 3324 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2052 10 Size: 54 1425 343 220 3473 Fragments arranged by size: 3473 1425 343 220 54 MslI CAynn'nnrTG Cuts at: 518 1166 2465 3473 3832 3991 518 Size: 648 1299 1008 359 159 2042 Fragments arranged by size: 2042 1299 1008 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2931 3608 4756 5001 1606 Size: 1325 677 1148 245 2120 Fragments arranged by size: 2120 1325 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4024 Fragments arranged by size: 4024 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5515 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5515 NotI GC'GGCC_GC Cuts at: 2962 2962 Size: 5515 NspI r_CATG'y Cuts at: 1132 1229 2873 4418 1132 Size: 97 1644 1545 2229 Fragments arranged by size: 2229 1644 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5515 PciI A'CATG_T Cuts at: 4414 4414 Size: 5515 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5515 PfoI T'CCnGG_A Cuts at: 2349 2349 Size: 5515 PmeI GTTT'AAAC Cuts at: 2819 2819 Size: 5515 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3584 3616 5097 5129 3584 Size: 32 1481 32 3970 Fragments arranged by size: 3970 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2289 2558 2289 Size: 269 5246 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5515 PsiI TTA'TAA Cuts at: 3085 3085 Size: 5515 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2705 2737 2705 Size: 32 5483 PstI C_TGCA'G Cuts at: 830 2867 830 Size: 2037 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5515 SalI G'TCGA_C Cuts at: 1936 2857 1936 Size: 921 4594 SapI GCTCTTCn'nnn_ Cuts at: 2589 2589 Size: 5515 SbfI CC_TGCA'GG Cuts at: 2867 2867 Size: 5515 ScaI AGT'ACT Cuts at: 3763 5475 3763 Size: 1712 3803 SfcI C'TryA_G Cuts at: 826 1045 2863 3998 4679 4870 826 Size: 219 1818 1135 681 191 1471 Fragments arranged by size: 1818 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5515 SmaI CCC'GGG Cuts at: 1872 2842 1872 Size: 970 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3619 4520 4782 820 Size: 197 926 1676 901 262 1553 Fragments arranged by size: 1676 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3306 493 Size: 2813 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5515 SphI G_CATG'C Cuts at: 1132 2873 1132 Size: 1741 3774 SspI AAT'ATT Cuts at: 5 52 3439 5 Size: 47 3387 2081 Fragments arranged by size: 3387 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5515 StyI C'CwwG_G Cuts at: 513 1065 2004 2088 2954 513 Size: 552 939 84 866 3074 Fragments arranged by size: 3074 939 866 552 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3542 3559 3712 3897 4342 325 Size: 1255 1962 17 153 185 445 1498 Fragments arranged by size: 1962 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2376 3761 5473 Size: 274 80 33 51 1443 399 1385 1712 Cuts at: 5473 96 Size: 138 Fragments arranged by size: 1712 1443 1385 399 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2384 2399 2416 2431 2619 2651 2656 2688 2736 Size: 15 17 15 188 32 5 32 48 Cuts at: 2736 2768 5475 5507 2384 Size: 32 2707 32 2392 Fragments arranged by size: 2707 2392 188 48 32 32 32 32 17 15 15 5 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5515 XbaI T'CTAG_A Cuts at: 2851 2851 Size: 5515 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2113 2113 Size: 5515 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5515 XmaI C'CCGG_G Cuts at: 1870 2840 1870 Size: 970 4545 XmnI GAAnn'nnTTC Cuts at: 3644 3644 Size: 5515 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3323 276 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI