################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AccI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI AspEI AspI AviII AvrII BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsiWI BspDI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DrdI Eam1105I EarI Eco47III Eco81I EcoO109I EcoO65I EcoRI EcoT22I FbaI FseI FspI HindIII HpaI Ksp632I KspI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SpeI SphI Sse8387I SwaI TaqI Tth111I TthHB8I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AatII 976 GACGTC GACGT^C Acc65I 869 GGTACC G^GTACC AccII 1471 CGCG CG^CG AccIII 300 TCCGGA T^CCGGA AclI 1090 AACGTT AA^CGTT AcsI 814 GAATTT [GA]^AATT[CT] Alw44I 218 GTGCAC G^TGCAC ApaLI 218 GTGCAC G^TGCAC ApoI 814 GAATTT [GA]^AATT[CT] Asp700I 847 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 869 GGTACC G^GTACC AvaI 85 CCCGAG C^[CT]CG[GA]G BamHI 1175 GGATCC G^GATCC BbeI 63 GGCGCC GGCGC^C BbrPI 1107 CACGTG CAC^GTG BbsI 922 GAAGAC GAAGAC(2/6) BglII 897 AGATCT A^GATCT BpuAI 922 GAAGAC GAAGAC(2/6) BsaBI 479 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1233 GGTCTC GGTCTC(1/5) BseAI 300 TCCGGA T^CCGGA BsiEI 112 CGGCCG CG[GA][CT]^CG BsmBI 978 CGTCTC CGTCTC(1/5) BsoBI 85 CCCGAG C^[CT]CG[GA]G Bsp1407I 1005 TGTACA T^GTACA BspEI 300 TCCGGA T^CCGGA BspMI 908 ACCTGC ACCTGC(4/8) BsrDI 471 CATTGC GCAATG(2/0) BsrGI 1005 TGTACA T^GTACA BstAPI 1344 GCACCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1471 CGCG CG^CG BstXI 1136 CCAACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 1345 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 112 CGGCCG C^GGCCG EclXI 112 CGGCCG C^GGCCG Eco52I 112 CGGCCG C^GGCCG EcoRV 148 GATATC GAT^ATC HincII 1114 GTCAAC GT[CT]^[GA]AC HindII 1114 GTCAAC GT[CT]^[GA]AC KasI 63 GGCGCC G^GCGCC KpnI 869 GGTACC GGTAC^C MamI 479 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 300 TCCGGA T^CCGGA MslI 457 CACGATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 1471 CGCG CG^CG NarI 63 GGCGCC GG^CGCC NcoI 1544 CCATGG C^CATGG PleI 925 GACTC GAGTC(4/5) PmaCI 1107 CACGTG CAC^GTG PmlI 1107 CACGTG CAC^GTG Psp1406I 1090 AACGTT AA^CGTT PvuII 1495 CAGCTG CAG^CTG SfcI 9 CTACAG C^T[GA][CT]AG SfoI 63 GGCGCC GGC^GCC SnaBI 526 TACGTA TAC^GTA SspBI 1005 TGTACA T^GTACA SspI 646 AATATT AAT^ATT StuI 1284 AGGCCT AGG^CCT XbaI 972 TCTAGA T^CTAGA XcmI 990 CCAAGTTGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 847 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AspHI 218 GTGCAC G[AT]GC[AT]^C 691 GAGCAC AvaII 662 GGACC G^G[AT]CC 1491 GGACC BalI 743 TGGCCA TGG^CCA 1547 TGGCCA BanII 104 GGGCTC G[GA]GC[CT]^C 1509 GAGCCC BbvCI 720 CCTCAGC CCTCAGC(-5/-2) 1119 CCTCAGC BpmI 415 CTGGAG CTGGAG(16/14) 893 CTGGAG BsaAI 526 TACGTA [CT]AC^GT[GA] 1107 CACGTG BsaWI 300 TCCGGA [AT]^CCGG[AT] 664 ACCGGA BsgI 1243 GTGCAG GTGCAG(16/14) 1351 GTGCAG BsiHKAI 218 GTGCAC G[AT]GC[AT]^C 691 GAGCAC BsmFI 506 GGGAC GGGAC(10/14) 521 GGGAC BsmI 572 GAATGC GAATGC(1/-1) 450 GCATTC BspLU11I 368 ACATGT A^CATGT 967 ACATGT EcoNI 590 CCTTTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 720 CCTCAGCCAGG EcoT14I 359 CCAAGG C^C[AT][AT]GG 1544 CCATGG HaeII 32 GGCGCT [GA]GCGC^[CT] 63 GGCGCC HgaI 1067 GACGC GACGC(5/10) 1540 GACGC MluNI 743 TGGCCA TGG^CCA 1547 TGGCCA MscI 743 TGGCCA TGG^CCA 1547 TGGCCA MseI 644 TTAA T^TAA 658 TTAA MspA1I 887 CCGCTG C[AC]G^C[GT]G 1495 CAGCTG StyI 359 CCAAGG C^C[AT][AT]GG 1544 CCATGG TfiI 390 GAATC G^A[AT]TC 544 GAATC Tru9I 644 TTAA T^TAA 658 TTAA Tsp509I 167 AATT ^AATT 815 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AcyI AfaI AflIII AluI AlwI AlwNI BanI BbvI BcnI BfaI BmyI BsaHI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I TspRI XhoII