(Circular) (Six-base) MAPSORT of: pFN21AE4158.seq Check: 1332 from: 1 to: 6301 pFN21AE4158 6301 bp With 182 enzymes: SgfI * March 28, 2012 13:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2985 4111 278 Size: 53 83 186 936 1449 1126 2468 Fragments arranged by size: 2468 1449 1126 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3644 1937 Size: 1707 4594 Acc65I G'GTAC_C Cuts at: 2874 3622 2874 Size: 748 5553 AclI AA'CG_TT Cuts at: 1563 3096 4428 4801 1563 Size: 1533 1332 373 3063 Fragments arranged by size: 3063 1533 1332 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4364 5748 6016 1632 Size: 2732 1384 268 1917 Fragments arranged by size: 2732 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6104 AflIII A'CryG_T Cuts at: 1203 2373 2972 3113 5200 6177 1203 Size: 1170 599 141 2087 977 1327 Fragments arranged by size: 2087 1327 1170 977 599 141 AgeI A'CCGG_T Cuts at: 5132 5132 Size: 6301 AhdI GACnn_n'nnGTC Cuts at: 5030 5030 Size: 6301 AleI CACnn'nnGTG Cuts at: 1166 2466 1166 Size: 1300 5001 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2829 1184 Size: 1645 4656 AlwNI CAG_nnn'CTG Cuts at: 2197 2759 3019 3328 4044 5616 2197 Size: 562 260 309 716 1572 2882 Fragments arranged by size: 2882 1572 716 562 309 260 ApaLI G'TGCA_C Cuts at: 2223 4358 5514 2223 Size: 2135 1156 3010 Fragments arranged by size: 3010 2135 1156 ApoI r'AATT_y Cuts at: 1493 2819 3610 3836 3988 6097 1493 Size: 1326 791 226 152 2109 1697 Fragments arranged by size: 2109 1697 1326 791 226 152 AseI AT'TA_AT Cuts at: 160 1032 4855 160 Size: 872 3823 1606 Fragments arranged by size: 3823 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 AvaI C'yCGr_G Cuts at: 1870 1943 2090 3626 1870 Size: 73 147 1536 4545 Fragments arranged by size: 4545 1536 147 73 BamHI G'GATC_C Cuts at: 3180 3631 3998 3180 Size: 451 367 5483 Fragments arranged by size: 5483 451 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2068 2298 2405 2874 3348 Size: 325 217 627 281 230 107 469 474 Cuts at: 3348 3622 5077 618 Size: 274 1455 1842 Fragments arranged by size: 1842 1455 627 474 469 325 281 274 230 217 107 BanII G_rGCy'C Cuts at: 1391 2113 3518 3620 1391 Size: 722 1405 102 4072 Fragments arranged by size: 4072 1405 722 102 BbeI G_GCGC'C Cuts at: 2072 2072 Size: 6301 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2934 928 Size: 971 1035 4295 Fragments arranged by size: 4295 1035 971 BbvCI CC'TCA_GC Cuts at: 2726 3125 2726 Size: 399 5902 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4492 4526 1903 Size: 34 2555 34 3678 Fragments arranged by size: 3678 2555 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4193 5403 4193 Size: 1210 5091 BclI T'GATC_A Cuts at: 1548 1548 Size: 6301 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4912 136 Size: 107 122 71 631 605 3240 1525 Fragments arranged by size: 3240 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2902 6296 2902 Size: 3394 2907 BlpI GC'TnA_GC Cuts at: 3718 3718 Size: 6301 Bme1580I G_kGCm'C Cuts at: 1790 2227 2301 3351 4362 5518 1790 Size: 437 74 1050 1011 1156 2573 Fragments arranged by size: 2573 1156 1050 1011 437 74 BmgBI CAC'GTC Cuts at: 1318 2241 1318 Size: 923 5378 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4032 4990 442 Size: 416 53 83 399 24 2615 958 1753 Fragments arranged by size: 2615 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6301 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2127 2127 Size: 6301 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2441 2919 4961 1239 Size: 1202 478 2042 2579 Fragments arranged by size: 2579 2042 1202 478 Bpu10I CC'TnA_GC Cuts at: 2726 3125 3233 2726 Size: 399 108 5794 Fragments arranged by size: 5794 399 108 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4426 5291 5589 4426 Size: 865 298 5138 Fragments arranged by size: 5138 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3244 4964 882 Size: 617 1745 1720 2219 Fragments arranged by size: 2219 1745 1720 617 BsaAI yAC'GTr Cuts at: 493 1204 2533 3114 4092 493 Size: 711 1329 581 978 2702 Fragments arranged by size: 2702 1329 978 711 581 BsaBI GATnn'nnATC Cuts at: 2488 6295 2488 Size: 3807 2494 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2069 2982 3073 3546 Size: 53 83 186 936 536 913 91 473 Cuts at: 3546 4108 4490 5125 275 Size: 562 382 635 1451 Fragments arranged by size: 1451 936 913 635 562 536 473 382 186 91 83 53 BsaWI w'CCGG_w Cuts at: 2305 2669 4734 5132 5406 5553 5964 2305 Size: 364 2065 398 274 147 411 2642 Fragments arranged by size: 2642 2065 411 398 364 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6076 Fragments arranged by size: 6076 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 2576 2807 1186 Size: 877 513 231 4680 Fragments arranged by size: 4680 877 513 231 BseYI C'CCAG_C Cuts at: 3088 5504 6000 3088 Size: 2416 496 3389 Fragments arranged by size: 3389 2416 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3269 3377 1163 Size: 573 1533 108 4087 Fragments arranged by size: 4087 1533 573 108 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2120 3751 4512 5540 1540 Size: 275 186 119 1631 761 1028 2301 Fragments arranged by size: 2301 1631 1028 761 275 186 119 BsiHKAI G_wGCw'C Cuts at: 2227 2700 4362 4447 5518 2227 Size: 473 1662 85 1071 3010 Fragments arranged by size: 3010 1662 1071 473 85 BsmI GAATG_Cn' Cuts at: 2455 2583 3812 3905 2455 Size: 128 1229 93 4851 Fragments arranged by size: 4851 1229 128 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2989 2989 Size: 6301 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2113 2227 2301 2700 3351 3518 3620 Size: 399 323 114 74 399 651 167 102 Cuts at: 3620 4362 4447 5518 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 651 399 399 323 167 114 102 85 74 BspEI T'CCGG_A Cuts at: 2305 5964 2305 Size: 3659 2642 BspHI T'CATG_A Cuts at: 4190 5920 4190 Size: 1730 4571 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2922 3656 844 Size: 2078 734 3489 Fragments arranged by size: 3489 2078 734 BsrBI CCG'CTC Cuts at: 1394 1823 4188 1394 Size: 429 2365 3507 Fragments arranged by size: 3507 2365 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2475 4796 4970 65 Size: 1183 1227 2321 174 1396 Fragments arranged by size: 2321 1396 1227 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4945 5132 1700 Size: 110 3135 187 2869 Fragments arranged by size: 3135 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3010 96 Size: 2914 3387 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6301 BssSI C'ACGA_G Cuts at: 4361 5373 4361 Size: 1012 5289 BstAPI GCAn_nnn'nTGC Cuts at: 794 3355 794 Size: 2561 3740 BstBI TT'CG_AA Cuts at: 5105 5105 Size: 6301 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6301 BstXI CCAn_nnnn'nTGG Cuts at: 3148 3148 Size: 6301 BstYI r'GATC_y Cuts at: 1972 2902 3045 3180 3631 3998 4383 4400 5852 Size: 930 143 135 451 367 385 17 1452 Cuts at: 5852 5938 5950 6296 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 930 451 385 367 346 143 135 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2745 2956 3549 513 Size: 552 939 741 211 593 3265 Fragments arranged by size: 3265 939 741 593 552 211 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3190 487 Size: 683 56 1964 3598 Fragments arranged by size: 3598 1964 683 56 BtsI GCAGTG_nn' Cuts at: 3202 3817 4611 4631 3202 Size: 615 794 20 4872 Fragments arranged by size: 4872 794 615 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6301 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6266 DraI TTT'AAA Cuts at: 3605 3605 Size: 6301 DraIII CAC_nnn'GTG Cuts at: 3355 3355 Size: 6301 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5308 809 Size: 507 3992 1802 Fragments arranged by size: 3992 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2117 2748 2988 Size: 54 1425 50 275 18 287 631 240 Cuts at: 2988 3393 3552 3748 4637 6266 8 Size: 405 159 196 889 1629 43 Fragments arranged by size: 1629 1425 889 631 405 287 275 240 196 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2117 3748 1537 Size: 275 305 1631 4090 Fragments arranged by size: 4090 1631 305 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4231 1353 Size: 2878 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2923 3211 4888 5262 5408 387 Size: 1064 138 1334 288 1677 374 146 1280 Fragments arranged by size: 1677 1334 1280 1064 374 288 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2441 2919 4364 4961 5748 6016 1239 Size: 393 809 478 1445 597 787 268 1524 Fragments arranged by size: 1524 1445 809 787 597 478 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2599 2729 2599 Size: 130 6171 EcoRI G'AATT_C Cuts at: 3610 3988 3610 Size: 378 5923 EcoRV GAT'ATC Cuts at: 2155 2155 Size: 6301 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2318 2362 2318 Size: 44 6257 FspI TGC'GCA Cuts at: 4807 4807 Size: 6301 HaeII r_GCGC'y Cuts at: 1678 2041 2072 5448 1678 Size: 363 31 3376 2531 Fragments arranged by size: 3376 2531 363 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3063 3095 4942 4974 5016 Size: 32 135 32 1491 32 1847 32 42 Cuts at: 5016 5048 1373 Size: 32 2626 Fragments arranged by size: 2626 1847 1491 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3121 3645 3891 669 Size: 988 281 1183 524 246 3079 Fragments arranged by size: 3079 1183 988 524 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6301 HpaI GTT'AAC Cuts at: 3891 3891 Size: 6301 KasI G'GCGC_C Cuts at: 2068 2068 Size: 6301 KpnI G_GTAC'C Cuts at: 2878 3626 2878 Size: 748 5553 MfeI C'AATT_G Cuts at: 3900 3900 Size: 6301 MluI A'CGCG_T Cuts at: 6177 6177 Size: 6301 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2182 5415 5599 1305 Size: 877 3233 184 2007 Fragments arranged by size: 3233 2007 877 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2750 3554 10 Size: 54 1425 343 918 804 2757 Fragments arranged by size: 2757 1425 918 804 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2466 4259 4618 4777 518 Size: 648 1300 1793 359 159 2042 Fragments arranged by size: 2042 1793 1300 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2894 3502 3717 4394 5542 5787 1606 Size: 1288 608 215 677 1148 245 2120 Fragments arranged by size: 2120 1288 1148 677 608 245 215 NarI GG'CG_CC Cuts at: 2069 2069 Size: 6301 NcoI C'CATG_G Cuts at: 513 1065 2004 3549 513 Size: 552 939 1545 3265 Fragments arranged by size: 3265 1545 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6301 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6301 NotI GC'GGCC_GC Cuts at: 3748 3748 Size: 6301 NspI r_CATG'y Cuts at: 1132 1229 2377 2429 2976 3659 5204 1132 Size: 97 1148 52 547 683 1545 2229 Fragments arranged by size: 2229 1545 1148 683 547 97 52 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6301 PciI A'CATG_T Cuts at: 2373 2972 5200 2373 Size: 599 2228 3474 Fragments arranged by size: 3474 2228 599 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6301 PfoI T'CCnGG_A Cuts at: 2544 2544 Size: 6301 PmeI GTTT'AAAC Cuts at: 3605 3605 Size: 6301 PmlI CAC'GTG Cuts at: 3114 3114 Size: 6301 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4370 4402 5883 5915 4370 Size: 32 1481 32 4756 Fragments arranged by size: 4756 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6301 PsiI TTA'TAA Cuts at: 3871 3871 Size: 6301 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2361 2393 2361 Size: 32 6269 PstI C_TGCA'G Cuts at: 830 3653 830 Size: 2823 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6301 PvuII CAG'CTG Cuts at: 3502 3502 Size: 6301 SalI G'TCGA_C Cuts at: 1936 3643 1936 Size: 1707 4594 SbfI CC_TGCA'GG Cuts at: 3653 3653 Size: 6301 ScaI AGT'ACT Cuts at: 4549 6261 4549 Size: 1712 4589 SfcI C'TryA_G Cuts at: 826 1045 2014 3649 4784 5465 5656 826 Size: 219 969 1635 1135 681 191 1471 Fragments arranged by size: 1635 1471 1135 969 681 219 191 SfoI GGC'GCC Cuts at: 2070 2070 Size: 6301 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6301 SmaI CCC'GGG Cuts at: 1872 3628 1872 Size: 1756 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4405 5306 5568 820 Size: 197 926 2462 901 262 1553 Fragments arranged by size: 2462 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2533 4092 493 Size: 2040 1559 2702 Fragments arranged by size: 2702 2040 1559 SpeI A'CTAG_T Cuts at: 152 152 Size: 6301 SphI G_CATG'C Cuts at: 1132 3659 1132 Size: 2527 3774 SspI AAT'ATT Cuts at: 5 52 2653 4225 5 Size: 47 2601 1572 2081 Fragments arranged by size: 2601 2081 1572 47 StuI AGG'CCT Cuts at: 1517 3291 1517 Size: 1774 4527 StyI C'CwwG_G Cuts at: 513 1065 2004 2364 3549 3740 513 Size: 552 939 360 1185 191 3074 Fragments arranged by size: 3074 1185 939 552 360 191 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3016 4328 4345 4498 4683 5128 325 Size: 1255 1436 1312 17 153 185 445 1498 Fragments arranged by size: 1498 1436 1312 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3010 4547 6259 Size: 274 80 33 51 1443 1033 1537 1712 Cuts at: 6259 96 Size: 138 Fragments arranged by size: 1712 1537 1443 1033 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6261 6293 6261 Size: 32 6269 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6301 XbaI T'CTAG_A Cuts at: 2977 3637 2977 Size: 660 5641 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3002 3002 Size: 6301 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6301 XmaI C'CCGG_G Cuts at: 1870 3626 1870 Size: 1756 4545 XmnI GAAnn'nnTTC Cuts at: 2856 4430 2856 Size: 1574 4727 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2983 4109 276 Size: 53 83 186 936 1449 1126 2468 Fragments arranged by size: 2468 1449 1126 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoO109I FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI