(Linear) (Six-base) MAPSORT of: KIEE4158.seq Check: 2110 from: 1 to: 1596 KIEE4158.orf With 182 enzymes: SgfI * March 22, 2012 17:29 .. AatII G_ACGT'C Cuts at: 0 980 1596 Size: 980 616 Acc65I G'GTAC_C Cuts at: 0 869 1596 Size: 869 727 AclI AA'CG_TT Cuts at: 0 1091 1596 Size: 1091 505 AflIII A'CryG_T Cuts at: 0 368 967 1108 1596 Size: 368 599 141 488 Fragments arranged by size: 599 488 368 141 AleI CACnn'nnGTG Cuts at: 0 461 1596 Size: 461 1135 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 824 1596 Size: 824 772 AlwNI CAG_nnn'CTG Cuts at: 0 192 754 1014 1323 1596 Size: 192 562 260 309 273 Fragments arranged by size: 562 309 273 260 192 ApaLI G'TGCA_C Cuts at: 0 218 1596 Size: 218 1378 ApoI r'AATT_y Cuts at: 0 814 1596 Size: 814 782 AvaI C'yCGr_G Cuts at: 0 85 1596 Size: 85 1511 BamHI G'GATC_C Cuts at: 0 1175 1596 Size: 1175 421 BanI G'GyrC_C Cuts at: 0 63 293 400 869 1343 1596 Size: 63 230 107 469 474 253 Fragments arranged by size: 474 469 253 230 107 63 BanII G_rGCy'C Cuts at: 0 108 1513 1596 Size: 108 1405 83 Fragments arranged by size: 1405 108 83 BbeI G_GCGC'C Cuts at: 0 67 1596 Size: 67 1529 BbsI GAAGACnn'nnnn_ Cuts at: 0 929 1596 Size: 929 667 BbvCI CC'TCA_GC Cuts at: 0 721 1120 1596 Size: 721 399 476 Fragments arranged by size: 721 476 399 BglII A'GATC_T Cuts at: 0 897 1596 Size: 897 699 Bme1580I G_kGCm'C Cuts at: 0 222 296 1346 1596 Size: 222 74 1050 250 Fragments arranged by size: 1050 250 222 74 BmgBI CAC'GTC Cuts at: 0 236 1596 Size: 236 1360 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 122 1596 Size: 122 1474 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 436 914 1596 Size: 436 478 682 Fragments arranged by size: 682 478 436 Bpu10I CC'TnA_GC Cuts at: 0 721 1120 1228 1596 Size: 721 399 108 368 Fragments arranged by size: 721 399 368 108 BsaI GGTCTCn'nnnn_ Cuts at: 0 1239 1596 Size: 1239 357 BsaAI yAC'GTr Cuts at: 0 528 1109 1596 Size: 528 581 487 Fragments arranged by size: 581 528 487 BsaBI GATnn'nnATC Cuts at: 0 483 1596 Size: 483 1113 BsaHI Gr'CG_yC Cuts at: 0 64 977 1068 1541 1596 Size: 64 913 91 473 55 Fragments arranged by size: 913 473 91 64 55 BsaWI w'CCGG_w Cuts at: 0 300 664 1596 Size: 300 364 932 Fragments arranged by size: 932 364 300 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 58 571 802 1596 Size: 58 513 231 794 Fragments arranged by size: 794 513 231 58 BseYI C'CCAG_C Cuts at: 0 1083 1596 Size: 1083 513 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1264 1372 1596 Size: 1264 108 224 Fragments arranged by size: 1264 224 108 BsiEI CG_ry'CG Cuts at: 0 115 1596 Size: 115 1481 BsiHKAI G_wGCw'C Cuts at: 0 222 695 1596 Size: 222 473 901 Fragments arranged by size: 901 473 222 BsmI GAATG_Cn' Cuts at: 0 450 578 1596 Size: 450 128 1018 Fragments arranged by size: 1018 450 128 BsmBI CGTCTCn'nnnn_ Cuts at: 0 984 1596 Size: 984 612 Bsp1286I G_dGCh'C Cuts at: 0 108 222 296 695 1346 1513 1596 Size: 108 114 74 399 651 167 83 Fragments arranged by size: 651 399 167 114 108 83 74 BspEI T'CCGG_A Cuts at: 0 300 1596 Size: 300 1296 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 917 1596 Size: 917 679 BsrDI GCAATG_nn' Cuts at: 0 470 1596 Size: 470 1126 BsrGI T'GTAC_A Cuts at: 0 1005 1596 Size: 1005 591 BstAPI GCAn_nnn'nTGC Cuts at: 0 1350 1596 Size: 1350 246 BstXI CCAn_nnnn'nTGG Cuts at: 0 1143 1596 Size: 1143 453 BstYI r'GATC_y Cuts at: 0 897 1040 1175 1596 Size: 897 143 135 421 Fragments arranged by size: 897 421 143 135 BtgI C'CryG_G Cuts at: 0 740 951 1544 1596 Size: 740 211 593 52 Fragments arranged by size: 740 593 211 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1185 1596 Size: 1185 411 BtsI GCAGTG_nn' Cuts at: 0 1197 1596 Size: 1197 399 DraIII CAC_nnn'GTG Cuts at: 0 1350 1596 Size: 1350 246 EaeI y'GGCC_r Cuts at: 0 112 743 983 1388 1547 1596 Size: 112 631 240 405 159 49 Fragments arranged by size: 631 405 240 159 112 49 EagI C'GGCC_G Cuts at: 0 112 1596 Size: 112 1484 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 918 1206 1596 Size: 918 288 390 Fragments arranged by size: 918 390 288 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 436 914 1596 Size: 436 478 682 Fragments arranged by size: 682 478 436 EcoNI CCTnn'n_nnAGG Cuts at: 0 594 724 1596 Size: 594 130 872 Fragments arranged by size: 872 594 130 EcoRV GAT'ATC Cuts at: 0 150 1596 Size: 150 1446 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 313 357 1596 Size: 313 44 1239 Fragments arranged by size: 1239 313 44 HaeII r_GCGC'y Cuts at: 0 36 67 1596 Size: 36 31 1529 Fragments arranged by size: 1529 36 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1058 1090 1596 Size: 1058 32 506 Fragments arranged by size: 1058 506 32 HincII GTy'rAC Cuts at: 0 1116 1596 Size: 1116 480 KasI G'GCGC_C Cuts at: 0 63 1596 Size: 63 1533 KpnI G_GTAC'C Cuts at: 0 873 1596 Size: 873 723 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 177 1596 Size: 177 1419 MscI TGG'CCA Cuts at: 0 745 1549 1596 Size: 745 804 47 Fragments arranged by size: 804 745 47 MslI CAynn'nnrTG Cuts at: 0 461 1596 Size: 461 1135 MspA1I CmG'CkG Cuts at: 0 889 1497 1596 Size: 889 608 99 Fragments arranged by size: 889 608 99 NarI GG'CG_CC Cuts at: 0 64 1596 Size: 64 1532 NcoI C'CATG_G Cuts at: 0 1544 1596 Size: 1544 52 NspI r_CATG'y Cuts at: 0 372 424 971 1596 Size: 372 52 547 625 Fragments arranged by size: 625 547 372 52 PciI A'CATG_T Cuts at: 0 368 967 1596 Size: 368 599 629 Fragments arranged by size: 629 599 368 PfoI T'CCnGG_A Cuts at: 0 539 1596 Size: 539 1057 PmlI CAC'GTG Cuts at: 0 1109 1596 Size: 1109 487 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 356 388 1596 Size: 356 32 1208 Fragments arranged by size: 1208 356 32 PvuII CAG'CTG Cuts at: 0 1497 1596 Size: 1497 99 SfcI C'TryA_G Cuts at: 0 9 1596 Size: 9 1587 SfoI GGC'GCC Cuts at: 0 65 1596 Size: 65 1531 SnaBI TAC'GTA Cuts at: 0 528 1596 Size: 528 1068 SspI AAT'ATT Cuts at: 0 648 1596 Size: 648 948 StuI AGG'CCT Cuts at: 0 1286 1596 Size: 1286 310 StyI C'CwwG_G Cuts at: 0 359 1544 1596 Size: 359 1185 52 Fragments arranged by size: 1185 359 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1011 1596 Size: 1011 585 TatI w'GTAC_w Cuts at: 0 1005 1596 Size: 1005 591 XbaI T'CTAG_A Cuts at: 0 972 1596 Size: 972 624 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 997 1596 Size: 997 599 XmnI GAAnn'nnTTC Cuts at: 0 851 1596 Size: 851 745 ZraI GAC'GTC Cuts at: 0 978 1596 Size: 978 618 Enzymes that do cut: AatII Acc65I AclI AflIII AleI AlfI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BglII Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI BsrGI BstAPI BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI EciI Eco57MI EcoNI EcoRV FalI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NarI NcoI NspI PciI PfoI PmlI PsrI PvuII SfcI SfoI SnaBI SspI StuI StyI TaqII TatI XbaI XcmI XmnI ZraI Enzymes that do not cut: AarI AccI AcuI AfeI AflII AgeI AhdI AloI ApaI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BglI BlpI BmrI BmtI BpuEI BsaXI BsiWI BspHI BsrBI BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DrdI EarI EcoICRI EcoO109I EcoRI FseI FspI FspAI HindIII HpaI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SpeI SphI SrfI SwaI TstI Tth111I XhoI XmaI