Restriction map for pFN21AE4158
 (Linear) (Six-base) MAPSORT of: KIEE4158.seq  Check: 2110  from: 1  to: 1596



KIEE4158.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:29  ..

AatII G_ACGT'C

Cuts at:      0     980    1596 
   Size:        980     616
 
Acc65I G'GTAC_C

Cuts at:      0     869    1596 
   Size:        869     727
 
AclI AA'CG_TT

Cuts at:      0    1091    1596 
   Size:       1091     505
 
AflIII A'CryG_T

Cuts at:      0     368     967    1108    1596 
   Size:        368     599     141     488

  Fragments arranged by size: 

                 599     488     368     141
 
AleI CACnn'nnGTG

Cuts at:      0     461    1596 
   Size:        461    1135
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     824    1596 
   Size:        824     772
 
AlwNI CAG_nnn'CTG

Cuts at:      0     192     754    1014    1323    1596 
   Size:        192     562     260     309     273

  Fragments arranged by size: 

                 562     309     273     260     192
 
ApaLI G'TGCA_C

Cuts at:      0     218    1596 
   Size:        218    1378
 
ApoI r'AATT_y

Cuts at:      0     814    1596 
   Size:        814     782
 
AvaI C'yCGr_G

Cuts at:      0      85    1596 
   Size:         85    1511
 
BamHI G'GATC_C

Cuts at:      0    1175    1596 
   Size:       1175     421
 
BanI G'GyrC_C

Cuts at:      0      63     293     400     869    1343    1596 
   Size:         63     230     107     469     474     253

  Fragments arranged by size: 

                 474     469     253     230     107      63
 
BanII G_rGCy'C

Cuts at:      0     108    1513    1596 
   Size:        108    1405      83

  Fragments arranged by size: 

                1405     108      83
 
BbeI G_GCGC'C

Cuts at:      0      67    1596 
   Size:         67    1529
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     929    1596 
   Size:        929     667
 
BbvCI CC'TCA_GC

Cuts at:      0     721    1120    1596 
   Size:        721     399     476

  Fragments arranged by size: 

                 721     476     399
 
BglII A'GATC_T

Cuts at:      0     897    1596 
   Size:        897     699
 
Bme1580I G_kGCm'C

Cuts at:      0     222     296    1346    1596 
   Size:        222      74    1050     250

  Fragments arranged by size: 

                1050     250     222      74
 
BmgBI CAC'GTC

Cuts at:      0     236    1596 
   Size:        236    1360
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     122    1596 
   Size:        122    1474
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436     914    1596 
   Size:        436     478     682

  Fragments arranged by size: 

                 682     478     436
 
Bpu10I CC'TnA_GC

Cuts at:      0     721    1120    1228    1596 
   Size:        721     399     108     368

  Fragments arranged by size: 

                 721     399     368     108
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1239    1596 
   Size:       1239     357
 
BsaAI yAC'GTr

Cuts at:      0     528    1109    1596 
   Size:        528     581     487

  Fragments arranged by size: 

                 581     528     487
 
BsaBI GATnn'nnATC

Cuts at:      0     483    1596 
   Size:        483    1113
 
BsaHI Gr'CG_yC

Cuts at:      0      64     977    1068    1541    1596 
   Size:         64     913      91     473      55

  Fragments arranged by size: 

                 913     473      91      64      55
 
BsaWI w'CCGG_w

Cuts at:      0     300     664    1596 
   Size:        300     364     932

  Fragments arranged by size: 

                 932     364     300
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      58     571     802    1596 
   Size:         58     513     231     794

  Fragments arranged by size: 

                 794     513     231      58
 
BseYI C'CCAG_C

Cuts at:      0    1083    1596 
   Size:       1083     513
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1264    1372    1596 
   Size:       1264     108     224

  Fragments arranged by size: 

                1264     224     108
 
BsiEI CG_ry'CG

Cuts at:      0     115    1596 
   Size:        115    1481
 
BsiHKAI G_wGCw'C

Cuts at:      0     222     695    1596 
   Size:        222     473     901

  Fragments arranged by size: 

                 901     473     222
 
BsmI GAATG_Cn'

Cuts at:      0     450     578    1596 
   Size:        450     128    1018

  Fragments arranged by size: 

                1018     450     128
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     984    1596 
   Size:        984     612
 
Bsp1286I G_dGCh'C

Cuts at:      0     108     222     296     695    1346    1513    1596 
   Size:        108     114      74     399     651     167      83

  Fragments arranged by size: 

                 651     399     167     114     108      83      74
 
BspEI T'CCGG_A

Cuts at:      0     300    1596 
   Size:        300    1296
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     917    1596 
   Size:        917     679
 
BsrDI GCAATG_nn'

Cuts at:      0     470    1596 
   Size:        470    1126
 
BsrGI T'GTAC_A

Cuts at:      0    1005    1596 
   Size:       1005     591
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1350    1596 
   Size:       1350     246
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1143    1596 
   Size:       1143     453
 
BstYI r'GATC_y

Cuts at:      0     897    1040    1175    1596 
   Size:        897     143     135     421

  Fragments arranged by size: 

                 897     421     143     135
 
BtgI C'CryG_G

Cuts at:      0     740     951    1544    1596 
   Size:        740     211     593      52

  Fragments arranged by size: 

                 740     593     211      52
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1185    1596 
   Size:       1185     411
 
BtsI GCAGTG_nn'

Cuts at:      0    1197    1596 
   Size:       1197     399
 
DraIII CAC_nnn'GTG

Cuts at:      0    1350    1596 
   Size:       1350     246
 
EaeI y'GGCC_r

Cuts at:      0     112     743     983    1388    1547    1596 
   Size:        112     631     240     405     159      49

  Fragments arranged by size: 

                 631     405     240     159     112      49
 
EagI C'GGCC_G

Cuts at:      0     112    1596 
   Size:        112    1484
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     918    1206    1596 
   Size:        918     288     390

  Fragments arranged by size: 

                 918     390     288
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436     914    1596 
   Size:        436     478     682

  Fragments arranged by size: 

                 682     478     436
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     594     724    1596 
   Size:        594     130     872

  Fragments arranged by size: 

                 872     594     130
 
EcoRV GAT'ATC

Cuts at:      0     150    1596 
   Size:        150    1446
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     313     357    1596 
   Size:        313      44    1239

  Fragments arranged by size: 

                1239     313      44
 
HaeII r_GCGC'y

Cuts at:      0      36      67    1596 
   Size:         36      31    1529

  Fragments arranged by size: 

                1529      36      31
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1058    1090    1596 
   Size:       1058      32     506

  Fragments arranged by size: 

                1058     506      32
 
HincII GTy'rAC

Cuts at:      0    1116    1596 
   Size:       1116     480
 
KasI G'GCGC_C

Cuts at:      0      63    1596 
   Size:         63    1533
 
KpnI G_GTAC'C

Cuts at:      0     873    1596 
   Size:        873     723
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     177    1596 
   Size:        177    1419
 
MscI TGG'CCA

Cuts at:      0     745    1549    1596 
   Size:        745     804      47

  Fragments arranged by size: 

                 804     745      47
 
MslI CAynn'nnrTG

Cuts at:      0     461    1596 
   Size:        461    1135
 
MspA1I CmG'CkG

Cuts at:      0     889    1497    1596 
   Size:        889     608      99

  Fragments arranged by size: 

                 889     608      99
 
NarI GG'CG_CC

Cuts at:      0      64    1596 
   Size:         64    1532
 
NcoI C'CATG_G

Cuts at:      0    1544    1596 
   Size:       1544      52
 
NspI r_CATG'y

Cuts at:      0     372     424     971    1596 
   Size:        372      52     547     625

  Fragments arranged by size: 

                 625     547     372      52
 
PciI A'CATG_T

Cuts at:      0     368     967    1596 
   Size:        368     599     629

  Fragments arranged by size: 

                 629     599     368
 
PfoI T'CCnGG_A

Cuts at:      0     539    1596 
   Size:        539    1057
 
PmlI CAC'GTG

Cuts at:      0    1109    1596 
   Size:       1109     487
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     356     388    1596 
   Size:        356      32    1208

  Fragments arranged by size: 

                1208     356      32
 
PvuII CAG'CTG

Cuts at:      0    1497    1596 
   Size:       1497      99
 
SfcI C'TryA_G

Cuts at:      0       9    1596 
   Size:          9    1587
 
SfoI GGC'GCC

Cuts at:      0      65    1596 
   Size:         65    1531
 
SnaBI TAC'GTA

Cuts at:      0     528    1596 
   Size:        528    1068
 
SspI AAT'ATT

Cuts at:      0     648    1596 
   Size:        648     948
 
StuI AGG'CCT

Cuts at:      0    1286    1596 
   Size:       1286     310
 
StyI C'CwwG_G

Cuts at:      0     359    1544    1596 
   Size:        359    1185      52

  Fragments arranged by size: 

                1185     359      52
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1011    1596 
   Size:       1011     585
 
TatI w'GTAC_w

Cuts at:      0    1005    1596 
   Size:       1005     591
 
XbaI T'CTAG_A

Cuts at:      0     972    1596 
   Size:        972     624
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     997    1596 
   Size:        997     599
 
XmnI GAAnn'nnTTC

Cuts at:      0     851    1596 
   Size:        851     745
 
ZraI GAC'GTC

Cuts at:      0     978    1596 
   Size:        978     618
 

 Enzymes that do cut:

    AatII   Acc65I     AclI   AflIII     AleI     AlfI    AlwNI    ApaLI
     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
    BglII Bme1580I    BmgBI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrDI    BsrGI   BstAPI
    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII     EaeI     EagI
     EciI  Eco57MI    EcoNI    EcoRV     FalI    HaeII    Hin4I   HincII
     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NarI     NcoI
     NspI     PciI     PfoI     PmlI     PsrI    PvuII     SfcI     SfoI
    SnaBI     SspI     StuI     StyI    TaqII     TatI     XbaI     XcmI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI     AcuI     AfeI    AflII     AgeI     AhdI     AloI
     ApaI     AscI     AseI    AsiSI    AvrII     BaeI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI     BmrI     BmtI    BpuEI    BsaXI
    BsiWI    BspHI    BsrBI    BsrFI   BssHII    BssSI    BstBI   BstEII
  BstZ17I   Bsu36I     ClaI    CspCI     DraI     DrdI     EarI  EcoICRI
 EcoO109I    EcoRI     FseI     FspI    FspAI  HindIII     HpaI     MfeI
     MluI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmaI     SmlI     SpeI     SphI     SrfI     SwaI     TstI  Tth111I
     XhoI     XmaI
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