Restriction map for pFN21AE1884
 (Circular) (Six-base) MAPSORT of: pFN21AE1884.seq  Check: 5479  from: 1  to: 5935



pFN21AE1884  5935 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:54  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3745     278 
   Size:         53      83     186     936    2209    2468

  Fragments arranged by size: 

                2468    2209     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2328    3278    1937 
   Size:        391     950    4594

  Fragments arranged by size: 

                4594     950     391
 
Acc65I G'GTAC_C

Cuts at:   3256    3256 
   Size:       5935
 
AclI AA'CG_TT

Cuts at:   1563    4062    4435    1563 
   Size:       2499     373    3063

  Fragments arranged by size: 

                3063    2499     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2021    2106    2750    2900    3998    5382    5650    1632 
   Size:        389      85     644     150    1098    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1098     644     389     268     150      85
            
 
AflII C'TTAA_G

Cuts at:    820    1017    2887     820 
   Size:        197    1870    3868

  Fragments arranged by size: 

                3868    1870     197
 
AflIII A'CryG_T

Cuts at:   1203    4834    5811    1203 
   Size:       3631     977    1327

  Fragments arranged by size: 

                3631    1327     977
 
AgeI A'CCGG_T

Cuts at:   4766    4766 
   Size:       5935
 
AhdI GACnn_n'nnGTC

Cuts at:   4664    4664 
   Size:       5935
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5935
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5935
 
AlwNI CAG_nnn'CTG

Cuts at:   2150    2354    3678    5250    2150 
   Size:        204    1324    1572    2835

  Fragments arranged by size: 

                2835    1572    1324     204
 
ApaLI G'TGCA_C

Cuts at:   3992    5148    3992 
   Size:       1156    4779
 
ApoI r'AATT_y

Cuts at:   1493    2624    2712    2756    3177    3244    3470    3622    5731 
   Size:       1131      88      44     421      67     226     152    2109

Cuts at:   5731    1493 
   Size:       1697

  Fragments arranged by size: 

                2109    1697    1131     421     226     152      88      67
                  44
 
AseI AT'TA_AT

Cuts at:    160    1032    4489     160 
   Size:        872    3457    1606

  Fragments arranged by size: 

                3457    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5935
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2425    2844    3260    1870 
   Size:         73     482     419     416    4545

  Fragments arranged by size: 

                4545     482     419     416      73
 
BamHI G'GATC_C

Cuts at:   3265    3632    3265 
   Size:        367    5568
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3256    4711     618 
   Size:        325     217     627    1469    1455    1842

  Fragments arranged by size: 

                1842    1469    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2508    2851    3254    1391 
   Size:       1117     343     403    4072

  Fragments arranged by size: 

                4072    1117     403     343
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4964
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4126    4160    1903 
   Size:         34    2189      34    3678

  Fragments arranged by size: 

                3678    2189      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3827    5037    3827 
   Size:       1210    4725
 
BclI T'GATC_A

Cuts at:   1548    2536    1548 
   Size:        988    4947
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4546     136 
   Size:        107     122      71     631     605    2874    1525

  Fragments arranged by size: 

                2874    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2608    5930    2608 
   Size:       3322    2613
 
BlpI GC'TnA_GC

Cuts at:   3352    3352 
   Size:       5935
 
Bme1580I G_kGCm'C

Cuts at:   1790    3996    5152    1790 
   Size:       2206    1156    2573

  Fragments arranged by size: 

                2573    2206    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5935
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3154    3666    4624 
   Size:        416      53      83     399      24    1737     512     958

Cuts at:   4624     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1737     958     512     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5935
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2599    4595    1239 
   Size:       1360    1996    2579

  Fragments arranged by size: 

                2579    1996    1360
 
Bpu10I CC'TnA_GC

Cuts at:   2154    2154 
   Size:       5935
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2031    4060    4925    5223    2031 
   Size:       2029     865     298    2743

  Fragments arranged by size: 

                2743    2029     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4598     882 
   Size:        617    3099    2219

  Fragments arranged by size: 

                3099    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3726     493 
   Size:        711    2522    2702

  Fragments arranged by size: 

                2702    2522     711
 
BsaBI GATnn'nnATC

Cuts at:   5929    5929 
   Size:       5935
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3742    4124    4759     275 
   Size:         53      83     186     936    2209     382     635    1451

  Fragments arranged by size: 

                2209    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4368    4766    5040    5187    5598    4368 
   Size:        398     274     147     411    4705

  Fragments arranged by size: 

                4705     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2727    2757    1178 
   Size:         30     165      30    1324      30    4356

  Fragments arranged by size: 

                4356    1324     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3129    1186 
   Size:       1943    3992
 
BseYI C'CCAG_C

Cuts at:   2143    5138    5634    2143 
   Size:       2995     496    2444

  Fragments arranged by size: 

                2995    2444     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5362
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3385    4146    5174    1540 
   Size:        275     186    1384     761    1028    2301

  Fragments arranged by size: 

                2301    1384    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2836    3996    4081    5152    2836 
   Size:       1160      85    1071    3619

  Fragments arranged by size: 

                3619    1160    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3135    3446    3539    3135 
   Size:        311      93    5531

  Fragments arranged by size: 

                5531     311      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2508    2836    2851    3254    3996    4081    5152 
   Size:        399     718     328      15     403     742      85    1071

Cuts at:   5152    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     718     403     399     328      85
                  15
 
BspEI T'CCGG_A

Cuts at:   5598    5598 
   Size:       5935
 
BspHI T'CATG_A

Cuts at:   3824    5554    3824 
   Size:       1730    4205
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3290     844 
   Size:       2446    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3822    1394 
   Size:        429    1999    3507

  Fragments arranged by size: 

                3507    1999     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4430    4604      65 
   Size:       1183    3182     174    1396

  Fragments arranged by size: 

                3182    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4579    4766    1700 
   Size:        110    2769     187    2869

  Fragments arranged by size: 

                2869    2769     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5935
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5935
 
BssSI C'ACGA_G

Cuts at:   3995    5007    3995 
   Size:       1012    4923
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2831     794 
   Size:       2037    3898
 
BstBI TT'CG_AA

Cuts at:   4739    4739 
   Size:       5935
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5935
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2581    2581 
   Size:       5935
 
BstYI r'GATC_y

Cuts at:   1972    2608    3265    3632    4017    4034    5486    5572    5584 
   Size:        636     657     367     385      17    1452      86      12

Cuts at:   5584    5930    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     657     636     385     367     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4444

  Fragments arranged by size: 

                4444     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5196

  Fragments arranged by size: 

                5196     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2691    3067    3155    3451    4245    4265    2691 
   Size:        376      88     296     794      20    4361

  Fragments arranged by size: 

                4361     794     376     296      88      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5935
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5900
 
DraI TTT'AAA

Cuts at:   2311    3239    2311 
   Size:        928    5007
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4942     809 
   Size:        507    3626    1802

  Fragments arranged by size: 

                3626    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3382    4271    5900 
   Size:         54    1425      50     275      18    1552     889    1629

Cuts at:   5900       8 
   Size:         43

  Fragments arranged by size: 

                1629    1552    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3382    1537 
   Size:        275    1570    4090

  Fragments arranged by size: 

                4090    1570     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3085    3865    1353 
   Size:       1732     780    3423

  Fragments arranged by size: 

                3423    1732     780
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4522    4896    5042     387 
   Size:       1064     138    2933     374     146    1280

  Fragments arranged by size: 

                2933    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2021    2106    2599    2750    2900    3998    4595 
   Size:        393     389      85     493     151     150    1098     597

Cuts at:   4595    5382    5650    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524    1098     787     597     493     393     389     268
                 151     150      85
 
EcoRI G'AATT_C

Cuts at:   3244    3622    3244 
   Size:        378    5557
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2375    2375 
   Size:       5935
 
FspI TGC'GCA

Cuts at:   4441    4441 
   Size:       5935
 
HaeII r_GCGC'y

Cuts at:   1678    5082    1678 
   Size:       3404    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2356    2380    2388    2412    4576 
   Size:         32     135      32     784      24       8      24    2164

Cuts at:   4576    4608    4650    4682    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    2164     784     135      42      32      32      32
                  32      24      24       8
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3279    3525     669 
   Size:        988     281    1341     246    3079

  Fragments arranged by size: 

                3079    1341     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2049     748 
   Size:       1301    4634
 
HpaI GTT'AAC

Cuts at:   3525    3525 
   Size:       5935
 
KpnI G_GTAC'C

Cuts at:   3260    3260 
   Size:       5935
 
MfeI C'AATT_G

Cuts at:   3534    3534 
   Size:       5935
 
MluI A'CGCG_T

Cuts at:   5811    5811 
   Size:       5935
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    5049    5233    1305 
   Size:       3744     184    2007

  Fragments arranged by size: 

                3744    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4113

  Fragments arranged by size: 

                4113    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2532    3893    4252    4411     518 
   Size:        648    1366    1361     359     159    2042

  Fragments arranged by size: 

                2042    1366    1361     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2521    3351    4028    5176    5421    1606 
   Size:        915     830     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     915     830     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4444

  Fragments arranged by size: 

                4444     939     552
 
NdeI CA'TA_TG

Cuts at:    387    2529    3028     387 
   Size:       2142     499    3294

  Fragments arranged by size: 

                3294    2142     499
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5935
 
NotI GC'GGCC_GC

Cuts at:   3382    3382 
   Size:       5935
 
NspI r_CATG'y

Cuts at:   1132    1229    3293    4838    1132 
   Size:         97    2064    1545    2229

  Fragments arranged by size: 

                2229    2064    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5935
 
PciI A'CATG_T

Cuts at:   4834    4834 
   Size:       5935
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5935
 
PmeI GTTT'AAAC

Cuts at:   3239    3239 
   Size:       5935
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4004    4036    5517    5549    4004 
   Size:         32    1481      32    4390

  Fragments arranged by size: 

                4390    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5935
 
PsiI TTA'TAA

Cuts at:   3505    3505 
   Size:       5935
 
PspXI vC'TCGA_Gb

Cuts at:   2844    2844 
   Size:       5935
 
PstI C_TGCA'G

Cuts at:    830    3287     830 
   Size:       2457    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5935
 
PvuII CAG'CTG

Cuts at:   2521    2521 
   Size:       5935
 
SalI G'TCGA_C

Cuts at:   1936    3277    1936 
   Size:       1341    4594
 
SbfI CC_TGCA'GG

Cuts at:   3287    3287 
   Size:       5935
 
ScaI AGT'ACT

Cuts at:   4183    5895    4183 
   Size:       1712    4223
 
SfcI C'TryA_G

Cuts at:    826    1045    2954    3283    4418    5099    5290     826 
   Size:        219    1909     329    1135     681     191    1471

  Fragments arranged by size: 

                1909    1471    1135     681     329     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5935
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5935
 
SmaI CCC'GGG

Cuts at:   1872    2427    3262    1872 
   Size:        555     835    4545

  Fragments arranged by size: 

                4545     835     555
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2046    2844    2887    4039    4940    5202 
   Size:        197     926     103     798      43    1152     901     262

Cuts at:   5202     820 
   Size:       1553

  Fragments arranged by size: 

                1553    1152     926     901     798     262     197     103
                  43
 
SnaBI TAC'GTA

Cuts at:    493    3726     493 
   Size:       3233    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5935
 
SphI G_CATG'C

Cuts at:   1132    3293    1132 
   Size:       2161    3774
 
SspI AAT'ATT

Cuts at:      5      52    3859       5 
   Size:         47    3807    2081

  Fragments arranged by size: 

                3807    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5935
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3374     513 
   Size:        552     939    1370    3074

  Fragments arranged by size: 

                3074    1370     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3962    3979    4132    4317    4762     325 
   Size:       1255    2382      17     153     185     445    1498

  Fragments arranged by size: 

                2382    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2650    4181    5893 
   Size:        274      80      33      51    1443     673    1531    1712

Cuts at:   5893      96 
   Size:        138

  Fragments arranged by size: 

                1712    1531    1443     673     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5895    5927    5895 
   Size:         32    5903
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2405    3160    1316 
   Size:       1089     755    4091

  Fragments arranged by size: 

                4091    1089     755
 
XbaI T'CTAG_A

Cuts at:   3271    3271 
   Size:       5935
 
XhoI C'TCGA_G

Cuts at:   1943    2844    1943 
   Size:        901    5034
 
XmaI C'CCGG_G

Cuts at:   1870    2425    3260    1870 
   Size:        555     835    4545

  Fragments arranged by size: 

                4545     835     555
 
XmnI GAAnn'nnTTC

Cuts at:   2133    4064    2133 
   Size:       1931    4004
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3743     276 
   Size:         53      83     186     936    2209    2468

  Fragments arranged by size: 

                2468    2209     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PshAI     PsiI    PspXI     PstI     PvuI    PvuII
     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI EcoO109I    EcoRV     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI    PpuMI
   PspOMI     PsrI    RsrII     SacI    SacII    SanDI     SapI    SexAI
     SfiI     SfoI     SrfI     SwaI     XcmI
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