Restriction map for
pFN21AE6465
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BanII
BbeI BbrPI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspEI BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HhaI HinP1I HindIII
HpaI HphI KasI KpnI KspI
MaeI MamI MluI MroI MspA1I
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AatII 168 GACGTC GACGT^C
AciI 209 GCGG CCGC(-3/-1)
AcyI 168 GACGTC G[GA]^CG[CT]C
AvaII 740 GGTCC G^G[AT]CC
BalI 656 TGGCCA TGG^CCA
BanI 630 GGCACC G^G[CT][GA]CC
BbsI 849 GAAGAC GAAGAC(2/6)
BbvCI 1435 GCTGAGG CCTCAGC(-5/-2)
BpuAI 849 GAAGAC GAAGAC(2/6)
BsaHI 168 GACGTC G[GA]^CG[CT]C
BsaI 907 GGTCTC GGTCTC(1/5)
BsmI 1190 GCATTC GAATGC(1/-1)
BspDI 106 ATCGAT AT^CGAT
BsrFI 159 GCCGGT [GA]^CCGG[CT]
BstAPI 691 GCAGTGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 159 GCCGGT [GA]^CCGG[CT]
ClaI 106 ATCGAT AT^CGAT
DraI 7 TTTAAA TTT^AAA
EaeI 656 TGGCCA [CT]^GGCC[GA]
EcoRI 566 GAATTC G^AATTC
HgaI 551 GCGTC GACGC(5/10)
Hin1I 168 GACGTC G[GA]^CG[CT]C
MfeI 964 CAATTG C^AATTG
MluNI 656 TGGCCA TGG^CCA
MscI 656 TGGCCA TGG^CCA
MunI 964 CAATTG C^AATTG
PstI 527 CTGCAG CTGCA^G
SapI 1567 GAAGAGC GCTCTTC(1/4)
SfaNI 548 GATGC GCATC(5/9)
StuI 803 AGGCCT AGG^CCT
Tsp45I 592 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 512 ACATGT A^C[GA][CT]GT
785 ACATGT
Asp700I 31 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1293 GAATGTGTTC
BamHI 272 GGATCC G^GATCC
372 GGATCC
BmyI 629 GGGCAC G[AGT]GC[ACT]^C
813 GTGCCC
BsaWI 275 TCCGGT [AT]^CCGG[AT]
342 ACCGGA
BsgI 163 GTGCAG GTGCAG(16/14)
724 GTGCAG
BsmAI 862 GAGAC GTCTC(1/5)
908 GTCTC
Bsp1286I 629 GGGCAC G[AGT]GC[ACT]^C
813 GTGCCC
BspHI 239 TCATGA T^CATGA
1022 TCATGA
BspLU11I 512 ACATGT A^CATGT
785 ACATGT
BsrI 368 ACTGG ACTGG(1/-1)
654 ACTGG
BstXI 701 CCATGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1085 CCAAAGGGGTGG
EarI 1567 GAAGAG CTCTTC(1/4)
1591 GAAGAG
EcoNI 952 CCTATTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1557 CCTTGATGAGG
EcoT14I 396 CCATGG C^C[AT][AT]GG
609 CCATGG
HincII 310 GTCAAC GT[CT]^[GA]AC
1156 GTCAAC
HindII 310 GTCAAC GT[CT]^[GA]AC
1156 GTCAAC
Ksp632I 1567 GAAGAG CTCTTC(1/4)
1591 GAAGAG
MaeII 169 ACGT A^CGT
887 ACGT
MaeIII 224 GTTAC ^GT[ACGT]AC
592 GTGAC
NcoI 396 CCATGG C^CATGG
609 CCATGG
PleI 504 GACTC GAGTC(4/5)
1470 GAGTC
RcaI 239 TCATGA T^CATGA
1022 TCATGA
SfcI 527 CTGCAG C^T[GA][CT]AG
1118 CTGTAG
StyI 396 CCATGG C^C[AT][AT]GG
609 CCATGG
TaqI 107 TCGA T^CGA
502 TCGA
TthHB8I 107 TCGA T^CGA
502 TCGA
XcmI 296 CCATTGTGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1187 CCAGCATTCTCATGG
XmnI 31 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1293 GAATGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BsaJI BseRI BsiYI BslI
BsmFI BsrDI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DsaI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScrFI TfiI Tru9I TseI Tsp509I
TspRI XhoII