(Circular) (Six-base) MAPSORT of: pFN21AE6465.seq Check: 6621 from: 1 to: 6322 pFN21AE6465 6322 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2177 4132 278 Size: 53 83 186 936 641 1955 2468 Fragments arranged by size: 2468 1955 936 641 186 83 53 AccI GT'mk_AC Cuts at: 1937 3665 1937 Size: 1728 4594 Acc65I G'GTAC_C Cuts at: 3643 3643 Size: 6322 AclI AA'CG_TT Cuts at: 1563 4449 4822 1563 Size: 2886 373 3063 Fragments arranged by size: 3063 2886 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4385 5769 6037 1632 Size: 2753 1384 268 1917 Fragments arranged by size: 2753 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6125 AflIII A'CryG_T Cuts at: 1203 2517 2790 5221 6198 1203 Size: 1314 273 2431 977 1327 Fragments arranged by size: 2431 1327 1314 977 273 AgeI A'CCGG_T Cuts at: 5153 5153 Size: 6322 AhdI GACnn_n'nnGTC Cuts at: 5051 5051 Size: 6322 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6322 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6322 AlwNI CAG_nnn'CTG Cuts at: 4065 5637 4065 Size: 1572 4750 ApaLI G'TGCA_C Cuts at: 4379 5535 4379 Size: 1156 5166 ApoI r'AATT_y Cuts at: 1493 2040 2094 2352 2436 2571 2997 3631 3857 Size: 547 54 258 84 135 426 634 226 Cuts at: 3857 4009 6118 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 634 547 426 258 226 152 135 84 54 AseI AT'TA_AT Cuts at: 160 1032 4876 160 Size: 872 3844 1606 Fragments arranged by size: 3844 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 AvaI C'yCGr_G Cuts at: 1870 1943 3647 1870 Size: 73 1704 4545 Fragments arranged by size: 4545 1704 73 BamHI G'GATC_C Cuts at: 2277 2377 3652 4019 2277 Size: 100 1275 367 4580 Fragments arranged by size: 4580 1275 367 100 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2635 3643 5098 618 Size: 325 217 627 848 1008 1455 1842 Fragments arranged by size: 1842 1455 1008 848 627 325 217 BanII G_rGCy'C Cuts at: 1391 3641 1391 Size: 2250 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2861 928 Size: 971 962 4389 Fragments arranged by size: 4389 971 962 BbvCI CC'TCA_GC Cuts at: 3441 3441 Size: 6322 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4513 4547 1903 Size: 34 50 34 2492 34 3678 Fragments arranged by size: 3678 2492 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4214 5424 4214 Size: 1210 5112 BclI T'GATC_A Cuts at: 1548 1548 Size: 6322 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4933 136 Size: 107 122 71 631 605 3261 1525 Fragments arranged by size: 3261 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6317 6317 Size: 6322 BlpI GC'TnA_GC Cuts at: 3739 3739 Size: 6322 Bme1580I G_kGCm'C Cuts at: 1790 2638 2822 4383 5539 1790 Size: 848 184 1561 1156 2573 Fragments arranged by size: 2573 1561 1156 848 184 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6322 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2383 4053 5011 Size: 416 53 83 399 24 966 1670 958 Cuts at: 5011 442 Size: 1753 Fragments arranged by size: 1753 1670 966 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6322 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3415 3415 Size: 6322 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4982 1239 Size: 3743 2579 Bpu10I CC'TnA_GC Cuts at: 3441 3441 Size: 6322 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4447 5312 5610 4447 Size: 865 298 5159 Fragments arranged by size: 5159 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2918 4985 882 Size: 617 1419 2067 2219 Fragments arranged by size: 2219 2067 1419 617 BsaAI yAC'GTr Cuts at: 493 1204 4113 493 Size: 711 2909 2702 Fragments arranged by size: 2909 2702 711 BsaBI GATnn'nnATC Cuts at: 6316 6316 Size: 6322 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2174 4129 4511 5146 Size: 53 83 186 936 641 1955 382 635 Cuts at: 5146 275 Size: 1451 Fragments arranged by size: 1955 1451 936 641 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2280 2347 4755 5153 5427 5574 5985 2280 Size: 67 2408 398 274 147 411 2617 Fragments arranged by size: 2617 2408 411 398 274 147 67 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6097 Fragments arranged by size: 6097 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2864 3419 3458 3567 1186 Size: 1678 555 39 109 3941 Fragments arranged by size: 3941 1678 555 109 39 BseYI C'CCAG_C Cuts at: 2254 2605 2717 2821 5525 6021 2254 Size: 351 112 104 2704 496 2555 Fragments arranged by size: 2704 2555 496 351 112 104 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2189 2750 1163 Size: 573 453 561 4735 Fragments arranged by size: 4735 573 561 453 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3772 4533 5561 1540 Size: 275 186 1771 761 1028 2301 Fragments arranged by size: 2301 1771 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4383 4468 5539 4383 Size: 85 1071 5166 Fragments arranged by size: 5166 1071 85 BsmI GAATG_Cn' Cuts at: 3195 3833 3926 3195 Size: 638 93 5591 Fragments arranged by size: 5591 638 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2638 2822 3641 4383 4468 5539 1391 Size: 399 848 184 819 742 85 1071 2174 Fragments arranged by size: 2174 1071 848 819 742 399 184 85 BspEI T'CCGG_A Cuts at: 5985 5985 Size: 6322 BspHI T'CATG_A Cuts at: 2244 3027 4211 5941 2244 Size: 783 1184 1730 2625 Fragments arranged by size: 2625 1730 1184 783 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3677 844 Size: 2833 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4209 1394 Size: 429 2386 3507 Fragments arranged by size: 3507 2386 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2235 3174 3270 4817 4991 65 Size: 1183 987 939 96 1547 174 1396 Fragments arranged by size: 1547 1396 1183 987 939 174 96 BsrFI r'CCGG_y Cuts at: 1700 1810 2164 4966 5153 1700 Size: 110 354 2802 187 2869 Fragments arranged by size: 2869 2802 354 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6322 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6322 BssSI C'ACGA_G Cuts at: 4382 5394 4382 Size: 1012 5310 BstAPI GCAn_nnn'nTGC Cuts at: 794 2702 794 Size: 1908 4414 BstBI TT'CG_AA Cuts at: 5126 5126 Size: 6322 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6322 BstXI CCAn_nnnn'nTGG Cuts at: 2713 3097 2713 Size: 384 5938 BstYI r'GATC_y Cuts at: 1972 2277 2377 2405 3207 3652 4019 4404 4421 Size: 305 100 28 802 445 367 385 17 Cuts at: 4421 5873 5959 5971 6317 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 802 445 385 367 346 305 100 86 28 17 12 BtgI C'CryG_G Cuts at: 513 1065 2076 2401 2614 513 Size: 552 1011 325 213 4221 Fragments arranged by size: 4221 1011 552 325 213 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5583 Fragments arranged by size: 5583 683 56 BtsI GCAGTG_nn' Cuts at: 2206 2703 3838 4632 4652 2206 Size: 497 1135 794 20 3876 Fragments arranged by size: 3876 1135 794 497 20 ClaI AT'CG_AT Cuts at: 1554 2112 1554 Size: 558 5764 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3201 3236 529 Size: 35 2637 35 3615 Fragments arranged by size: 3615 2637 35 35 DraI TTT'AAA Cuts at: 2014 3626 2014 Size: 1612 4710 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5329 809 Size: 507 4013 1802 Fragments arranged by size: 4013 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2661 3769 4658 Size: 54 1425 50 275 18 831 1108 889 Cuts at: 4658 6287 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1108 889 831 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3769 1537 Size: 275 1957 4090 Fragments arranged by size: 4090 1957 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3567 3591 4252 1353 Size: 2214 24 661 3423 Fragments arranged by size: 3423 2214 661 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4909 5283 5429 387 Size: 1064 138 3320 374 146 1280 Fragments arranged by size: 3320 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4385 4982 5769 6037 1239 Size: 393 2753 597 787 268 1524 Fragments arranged by size: 2753 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2961 3566 2961 Size: 605 5717 EcoRI G'AATT_C Cuts at: 2571 3631 4009 2571 Size: 1060 378 4884 Fragments arranged by size: 4884 1060 378 FspI TGC'GCA Cuts at: 4828 4828 Size: 6322 HaeII r_GCGC'y Cuts at: 1678 5469 1678 Size: 3791 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2478 2510 3566 3598 4963 Size: 32 135 32 906 32 1056 32 1365 Cuts at: 4963 4995 5037 5069 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1365 1056 906 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2317 3163 3666 3912 669 Size: 988 281 379 846 503 246 3079 Fragments arranged by size: 3079 988 846 503 379 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6322 HpaI GTT'AAC Cuts at: 3912 3912 Size: 6322 KpnI G_GTAC'C Cuts at: 3647 3647 Size: 6322 MfeI C'AATT_G Cuts at: 2969 3921 2969 Size: 952 5370 MluI A'CGCG_T Cuts at: 6198 6198 Size: 6322 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2858 5436 5620 1305 Size: 1553 2578 184 2007 Fragments arranged by size: 2578 2007 1553 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2663 10 Size: 54 1425 343 831 3669 Fragments arranged by size: 3669 1425 831 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2939 3200 3330 4280 4639 4798 518 Size: 648 1773 261 130 950 359 159 2042 Fragments arranged by size: 2042 1773 950 648 359 261 159 130 MspA1I CmG'CkG Cuts at: 1606 3738 4415 5563 5808 1606 Size: 2132 677 1148 245 2120 Fragments arranged by size: 2132 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2401 2614 513 Size: 552 1336 213 4221 Fragments arranged by size: 4221 1336 552 213 NdeI CA'TA_TG Cuts at: 387 387 Size: 6322 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6322 NotI GC'GGCC_GC Cuts at: 3769 3769 Size: 6322 NspI r_CATG'y Cuts at: 1132 1229 2521 2773 2794 3680 5225 1132 Size: 97 1292 252 21 886 1545 2229 Fragments arranged by size: 2229 1545 1292 886 252 97 21 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6322 PciI A'CATG_T Cuts at: 2517 2790 5221 2517 Size: 273 2431 3618 Fragments arranged by size: 3618 2431 273 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6322 PmeI GTTT'AAAC Cuts at: 3626 3626 Size: 6322 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4391 4423 5904 5936 4391 Size: 32 1481 32 4777 Fragments arranged by size: 4777 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6322 PsiI TTA'TAA Cuts at: 3892 3892 Size: 6322 PstI C_TGCA'G Cuts at: 830 2536 3674 830 Size: 1706 1138 3478 Fragments arranged by size: 3478 1706 1138 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6322 SalI G'TCGA_C Cuts at: 1936 3664 1936 Size: 1728 4594 SapI GCTCTTCn'nnn_ Cuts at: 3567 3567 Size: 6322 SbfI CC_TGCA'GG Cuts at: 3674 3674 Size: 6322 ScaI AGT'ACT Cuts at: 4570 6282 4570 Size: 1712 4610 SfcI C'TryA_G Cuts at: 826 1045 2532 3123 3670 4805 5486 5677 826 Size: 219 1487 591 547 1135 681 191 1471 Fragments arranged by size: 1487 1471 1135 681 591 547 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6322 SmaI CCC'GGG Cuts at: 1872 3649 1872 Size: 1777 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4426 5327 5589 820 Size: 197 926 2483 901 262 1553 Fragments arranged by size: 2483 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4113 493 Size: 3620 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6322 SphI G_CATG'C Cuts at: 1132 3680 1132 Size: 2548 3774 SspI AAT'ATT Cuts at: 5 52 4246 5 Size: 47 4194 2081 Fragments arranged by size: 4194 2081 47 StuI AGG'CCT Cuts at: 1517 2810 1517 Size: 1293 5029 StyI C'CwwG_G Cuts at: 513 1065 2401 2614 3761 513 Size: 552 1336 213 1147 3074 Fragments arranged by size: 3074 1336 1147 552 213 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4349 4366 4519 4704 5149 325 Size: 1255 2769 17 153 185 445 1498 Fragments arranged by size: 2769 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2733 4568 6280 Size: 274 80 33 51 1443 756 1835 1712 Cuts at: 6280 96 Size: 138 Fragments arranged by size: 1835 1712 1443 756 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2151 2183 6282 6314 2151 Size: 32 4099 32 2159 Fragments arranged by size: 4099 2159 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6322 XbaI T'CTAG_A Cuts at: 3658 3658 Size: 6322 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2308 3199 2308 Size: 891 5431 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6322 XmaI C'CCGG_G Cuts at: 1870 3647 1870 Size: 1777 4545 XmnI GAAnn'nnTTC Cuts at: 2040 3302 4451 2040 Size: 1262 1149 3911 Fragments arranged by size: 3911 1262 1149 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2175 4130 276 Size: 53 83 186 936 641 1955 2468 Fragments arranged by size: 2468 1955 936 641 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI