Restriction map for pFN21AE6465
 (Linear) (Six-base) MAPSORT of: KIEE6465.seq  Check: 873  from: 1  to: 1617



KIEE6465.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:32  ..

AatII G_ACGT'C

Cuts at:      0     172    1617 
   Size:        172    1445
 
AflIII A'CryG_T

Cuts at:      0     512     785    1617 
   Size:        512     273     832

  Fragments arranged by size: 

                 832     512     273
 
ApoI r'AATT_y

Cuts at:      0      35      89     347     431     566     992    1617 
   Size:         35      54     258      84     135     426     625

  Fragments arranged by size: 

                 625     426     258     135      84      54      35
 
BamHI G'GATC_C

Cuts at:      0     272     372    1617 
   Size:        272     100    1245

  Fragments arranged by size: 

                1245     272     100
 
BanI G'GyrC_C

Cuts at:      0     630    1617 
   Size:        630     987
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     856    1617 
   Size:        856     761
 
BbvCI CC'TCA_GC

Cuts at:      0    1436    1617 
   Size:       1436     181
 
Bme1580I G_kGCm'C

Cuts at:      0     633     817    1617 
   Size:        633     184     800

  Fragments arranged by size: 

                 800     633     184
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     378    1617 
   Size:        378    1239
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1410    1617 
   Size:       1410     207
 
Bpu10I CC'TnA_GC

Cuts at:      0    1436    1617 
   Size:       1436     181
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     913    1617 
   Size:        913     704
 
BsaHI Gr'CG_yC

Cuts at:      0     169    1617 
   Size:        169    1448
 
BsaWI w'CCGG_w

Cuts at:      0     275     342    1617 
   Size:        275      67    1275

  Fragments arranged by size: 

                1275     275      67
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     859    1414    1453    1562    1617 
   Size:        859     555      39     109      55

  Fragments arranged by size: 

                 859     555     109      55      39
 
BseYI C'CCAG_C

Cuts at:      0     249     600     712     816    1617 
   Size:        249     351     112     104     801

  Fragments arranged by size: 

                 801     351     249     112     104
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     745    1617 
   Size:        184     561     872

  Fragments arranged by size: 

                 872     561     184
 
BsmI GAATG_Cn'

Cuts at:      0    1190    1617 
   Size:       1190     427
 
Bsp1286I G_dGCh'C

Cuts at:      0     633     817    1617 
   Size:        633     184     800

  Fragments arranged by size: 

                 800     633     184
 
BspHI T'CATG_A

Cuts at:      0     239    1022    1617 
   Size:        239     783     595

  Fragments arranged by size: 

                 783     595     239
 
BsrDI GCAATG_nn'

Cuts at:      0     230    1169    1265    1617 
   Size:        230     939      96     352

  Fragments arranged by size: 

                 939     352     230      96
 
BsrFI r'CCGG_y

Cuts at:      0     159    1617 
   Size:        159    1458
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     697    1617 
   Size:        697     920
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     708    1092    1617 
   Size:        708     384     525

  Fragments arranged by size: 

                 708     525     384
 
BstYI r'GATC_y

Cuts at:      0     272     372     400    1202    1617 
   Size:        272     100      28     802     415

  Fragments arranged by size: 

                 802     415     272     100      28
 
BtgI C'CryG_G

Cuts at:      0      71     396     609    1617 
   Size:         71     325     213    1008

  Fragments arranged by size: 

                1008     325     213      71
 
BtsI GCAGTG_nn'

Cuts at:      0     201     698    1617 
   Size:        201     497     919

  Fragments arranged by size: 

                 919     497     201
 
ClaI AT'CG_AT

Cuts at:      0     107    1617 
   Size:        107    1510
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1196    1231    1617 
   Size:       1196      35     386

  Fragments arranged by size: 

                1196     386      35
 
DraI TTT'AAA

Cuts at:      0       9    1617 
   Size:          9    1608
 
EaeI y'GGCC_r

Cuts at:      0     656    1617 
   Size:        656     961
 
EarI CTCTTCn'nnn_

Cuts at:      0    1562    1586    1617 
   Size:       1562      24      31

  Fragments arranged by size: 

                1562      31      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     956    1561    1617 
   Size:        956     605      56

  Fragments arranged by size: 

                 956     605      56
 
EcoRI G'AATT_C

Cuts at:      0     566    1617 
   Size:        566    1051
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     473     505    1561    1593    1617 
   Size:        473      32    1056      32      24

  Fragments arranged by size: 

                1056     473      32      32      24
 
HincII GTy'rAC

Cuts at:      0     312    1158    1617 
   Size:        312     846     459

  Fragments arranged by size: 

                 846     459     312
 
MfeI C'AATT_G

Cuts at:      0     964    1617 
   Size:        964     653
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     853    1617 
   Size:        853     764
 
MscI TGG'CCA

Cuts at:      0     658    1617 
   Size:        658     959
 
MslI CAynn'nnrTG

Cuts at:      0     934    1195    1325    1617 
   Size:        934     261     130     292

  Fragments arranged by size: 

                 934     292     261     130
 
NcoI C'CATG_G

Cuts at:      0     396     609    1617 
   Size:        396     213    1008

  Fragments arranged by size: 

                1008     396     213
 
NspI r_CATG'y

Cuts at:      0     516     768     789    1617 
   Size:        516     252      21     828

  Fragments arranged by size: 

                 828     516     252      21
 
PciI A'CATG_T

Cuts at:      0     512     785    1617 
   Size:        512     273     832

  Fragments arranged by size: 

                 832     512     273
 
PstI C_TGCA'G

Cuts at:      0     531    1617 
   Size:        531    1086
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1562    1617 
   Size:       1562      55
 
SfcI C'TryA_G

Cuts at:      0     527    1118    1617 
   Size:        527     591     499

  Fragments arranged by size: 

                 591     527     499
 
StuI AGG'CCT

Cuts at:      0     805    1617 
   Size:        805     812
 
StyI C'CwwG_G

Cuts at:      0     396     609    1617 
   Size:        396     213    1008

  Fragments arranged by size: 

                1008     396     213
 
TatI w'GTAC_w

Cuts at:      0     728    1617 
   Size:        728     889
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     146     178    1617 
   Size:        146      32    1439

  Fragments arranged by size: 

                1439     146      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     303    1194    1617 
   Size:        303     891     423

  Fragments arranged by size: 

                 891     423     303
 
XmnI GAAnn'nnTTC

Cuts at:      0      35    1297    1617 
   Size:         35    1262     320

  Fragments arranged by size: 

                1262     320      35
 
ZraI GAC'GTC

Cuts at:      0     170    1617 
   Size:        170    1447
 

 Enzymes that do cut:

    AatII   AflIII     ApoI    BamHI     BanI     BbsI    BbvCI Bme1580I
     BmrI     BplI   Bpu10I     BsaI    BsaHI    BsaWI    BseRI    BseYI
     BsgI     BsmI Bsp1286I    BspHI    BsrDI    BsrFI   BstAPI    BstXI
    BstYI     BtgI     BtsI     ClaI    CspCI     DraI     EaeI     EarI
    EcoNI    EcoRI    Hin4I   HincII     MfeI     MmeI     MscI     MslI
     NcoI     NspI     PciI     PstI     SapI     SfcI     StuI     StyI
     TatI     TstI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BpmI    BpuEI    BsaAI    BsaBI    BsaXI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspEI    BspMI    BsrBI    BsrGI   BssHII    BssSI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI   DraIII     DrdI     EagI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MluI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII  Tth111I     XbaI     XhoI     XmaI
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