(Linear) (Six-base) MAPSORT of: KIEE6465.seq Check: 873 from: 1 to: 1617 KIEE6465.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AatII G_ACGT'C Cuts at: 0 172 1617 Size: 172 1445 AflIII A'CryG_T Cuts at: 0 512 785 1617 Size: 512 273 832 Fragments arranged by size: 832 512 273 ApoI r'AATT_y Cuts at: 0 35 89 347 431 566 992 1617 Size: 35 54 258 84 135 426 625 Fragments arranged by size: 625 426 258 135 84 54 35 BamHI G'GATC_C Cuts at: 0 272 372 1617 Size: 272 100 1245 Fragments arranged by size: 1245 272 100 BanI G'GyrC_C Cuts at: 0 630 1617 Size: 630 987 BbsI GAAGACnn'nnnn_ Cuts at: 0 856 1617 Size: 856 761 BbvCI CC'TCA_GC Cuts at: 0 1436 1617 Size: 1436 181 Bme1580I G_kGCm'C Cuts at: 0 633 817 1617 Size: 633 184 800 Fragments arranged by size: 800 633 184 BmrI ACTGGGnnnn_n' Cuts at: 0 378 1617 Size: 378 1239 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1410 1617 Size: 1410 207 Bpu10I CC'TnA_GC Cuts at: 0 1436 1617 Size: 1436 181 BsaI GGTCTCn'nnnn_ Cuts at: 0 913 1617 Size: 913 704 BsaHI Gr'CG_yC Cuts at: 0 169 1617 Size: 169 1448 BsaWI w'CCGG_w Cuts at: 0 275 342 1617 Size: 275 67 1275 Fragments arranged by size: 1275 275 67 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 859 1414 1453 1562 1617 Size: 859 555 39 109 55 Fragments arranged by size: 859 555 109 55 39 BseYI C'CCAG_C Cuts at: 0 249 600 712 816 1617 Size: 249 351 112 104 801 Fragments arranged by size: 801 351 249 112 104 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 745 1617 Size: 184 561 872 Fragments arranged by size: 872 561 184 BsmI GAATG_Cn' Cuts at: 0 1190 1617 Size: 1190 427 Bsp1286I G_dGCh'C Cuts at: 0 633 817 1617 Size: 633 184 800 Fragments arranged by size: 800 633 184 BspHI T'CATG_A Cuts at: 0 239 1022 1617 Size: 239 783 595 Fragments arranged by size: 783 595 239 BsrDI GCAATG_nn' Cuts at: 0 230 1169 1265 1617 Size: 230 939 96 352 Fragments arranged by size: 939 352 230 96 BsrFI r'CCGG_y Cuts at: 0 159 1617 Size: 159 1458 BstAPI GCAn_nnn'nTGC Cuts at: 0 697 1617 Size: 697 920 BstXI CCAn_nnnn'nTGG Cuts at: 0 708 1092 1617 Size: 708 384 525 Fragments arranged by size: 708 525 384 BstYI r'GATC_y Cuts at: 0 272 372 400 1202 1617 Size: 272 100 28 802 415 Fragments arranged by size: 802 415 272 100 28 BtgI C'CryG_G Cuts at: 0 71 396 609 1617 Size: 71 325 213 1008 Fragments arranged by size: 1008 325 213 71 BtsI GCAGTG_nn' Cuts at: 0 201 698 1617 Size: 201 497 919 Fragments arranged by size: 919 497 201 ClaI AT'CG_AT Cuts at: 0 107 1617 Size: 107 1510 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1196 1231 1617 Size: 1196 35 386 Fragments arranged by size: 1196 386 35 DraI TTT'AAA Cuts at: 0 9 1617 Size: 9 1608 EaeI y'GGCC_r Cuts at: 0 656 1617 Size: 656 961 EarI CTCTTCn'nnn_ Cuts at: 0 1562 1586 1617 Size: 1562 24 31 Fragments arranged by size: 1562 31 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 956 1561 1617 Size: 956 605 56 Fragments arranged by size: 956 605 56 EcoRI G'AATT_C Cuts at: 0 566 1617 Size: 566 1051 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 473 505 1561 1593 1617 Size: 473 32 1056 32 24 Fragments arranged by size: 1056 473 32 32 24 HincII GTy'rAC Cuts at: 0 312 1158 1617 Size: 312 846 459 Fragments arranged by size: 846 459 312 MfeI C'AATT_G Cuts at: 0 964 1617 Size: 964 653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 853 1617 Size: 853 764 MscI TGG'CCA Cuts at: 0 658 1617 Size: 658 959 MslI CAynn'nnrTG Cuts at: 0 934 1195 1325 1617 Size: 934 261 130 292 Fragments arranged by size: 934 292 261 130 NcoI C'CATG_G Cuts at: 0 396 609 1617 Size: 396 213 1008 Fragments arranged by size: 1008 396 213 NspI r_CATG'y Cuts at: 0 516 768 789 1617 Size: 516 252 21 828 Fragments arranged by size: 828 516 252 21 PciI A'CATG_T Cuts at: 0 512 785 1617 Size: 512 273 832 Fragments arranged by size: 832 512 273 PstI C_TGCA'G Cuts at: 0 531 1617 Size: 531 1086 SapI GCTCTTCn'nnn_ Cuts at: 0 1562 1617 Size: 1562 55 SfcI C'TryA_G Cuts at: 0 527 1118 1617 Size: 527 591 499 Fragments arranged by size: 591 527 499 StuI AGG'CCT Cuts at: 0 805 1617 Size: 805 812 StyI C'CwwG_G Cuts at: 0 396 609 1617 Size: 396 213 1008 Fragments arranged by size: 1008 396 213 TatI w'GTAC_w Cuts at: 0 728 1617 Size: 728 889 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 146 178 1617 Size: 146 32 1439 Fragments arranged by size: 1439 146 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 303 1194 1617 Size: 303 891 423 Fragments arranged by size: 891 423 303 XmnI GAAnn'nnTTC Cuts at: 0 35 1297 1617 Size: 35 1262 320 Fragments arranged by size: 1262 320 35 ZraI GAC'GTC Cuts at: 0 170 1617 Size: 170 1447 Enzymes that do cut: AatII AflIII ApoI BamHI BanI BbsI BbvCI Bme1580I BmrI BplI Bpu10I BsaI BsaHI BsaWI BseRI BseYI BsgI BsmI Bsp1286I BspHI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtsI ClaI CspCI DraI EaeI EarI EcoNI EcoRI Hin4I HincII MfeI MmeI MscI MslI NcoI NspI PciI PstI SapI SfcI StuI StyI TatI TstI XcmI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanII BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BpmI BpuEI BsaAI BsaBI BsaXI BsiEI BsiHKAI BsiWI BsmBI BspEI BspMI BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI DraIII DrdI EagI EciI EcoICRI Eco57MI EcoO109I EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MluI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI XhoI XmaI