################################## # NO CUT ENZYMES ( 234 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI KspI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI ScrFI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 16 enzymes) # ################################### AfaI 84 GTAC GT^AC AflII 67 CTTAAG C^TTAAG BbvI 126 GCAGC GCAGC(8/12) BfrI 67 CTTAAG C^TTAAG BsrI 52 CCAGT ACTGG(1/-1) Cac8I 129 GCTTGC GC[ACGT]^[ACGT]GC EarI 229 GAAGAG CTCTTC(1/4) Fnu4HI 126 GCAGC GC^[ACGT]GC ItaI 126 GCAGC GC^[ACGT]GC Ksp632I 229 GAAGAG CTCTTC(1/4) NspI 122 ACATGC [GA]CATG^[CT] RsaI 84 GTAC GT^AC SfcI 104 CTGTAG C^T[GA][CT]AG SmlI 67 CTTAAG C^T[CT][GA]AG TseI 126 GCAGC G^C[AT]GC TspRI 53 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 6 enzymes) # ################################### AluI 128 AGCT AG^CT 159 AGCT HphI 60 GGTGA GGTGA(8/7) 178 GGTGA MseI 68 TTAA T^TAA 187 TTAA MslI 117 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 172 CATCTGGGTG NlaIII 117 CATG CATG^ 123 CATG Tru9I 68 TTAA T^TAA 187 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### MboII MnlI