(Circular) (Six-base) MAPSORT of: pFN21AE5150.seq Check: 3112 from: 1 to: 4963 pFN21AE5150 4963 bp With 182 enzymes: SgfI * January 30, 2014 17:13 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2773 278 Size: 53 83 186 936 1237 2468 Fragments arranged by size: 2468 1237 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2306 1937 Size: 369 4594 Acc65I G'GTAC_C Cuts at: 2284 2284 Size: 4963 AclI AA'CG_TT Cuts at: 1563 3090 3463 1563 Size: 1527 373 3063 Fragments arranged by size: 3063 1527 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3026 4410 4678 1632 Size: 1394 1384 268 1917 Fragments arranged by size: 1917 1394 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2072 820 Size: 197 1055 3711 Fragments arranged by size: 3711 1055 197 AflIII A'CryG_T Cuts at: 1203 3862 4839 1203 Size: 2659 977 1327 Fragments arranged by size: 2659 1327 977 AgeI A'CCGG_T Cuts at: 3794 3794 Size: 4963 AhdI GACnn_n'nnGTC Cuts at: 3692 3692 Size: 4963 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4963 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4963 AlwNI CAG_nnn'CTG Cuts at: 2706 4278 2706 Size: 1572 3391 ApaLI G'TGCA_C Cuts at: 3020 4176 3020 Size: 1156 3807 ApoI r'AATT_y Cuts at: 1493 2272 2498 2650 4759 1493 Size: 779 226 152 2109 1697 Fragments arranged by size: 2109 1697 779 226 152 AseI AT'TA_AT Cuts at: 160 1032 3517 160 Size: 872 2485 1606 Fragments arranged by size: 2485 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4963 AvaI C'yCGr_G Cuts at: 1870 1943 2288 1870 Size: 73 345 4545 Fragments arranged by size: 4545 345 73 BamHI G'GATC_C Cuts at: 2293 2660 2293 Size: 367 4596 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2284 3739 618 Size: 325 217 627 497 1455 1842 Fragments arranged by size: 1842 1455 627 497 325 217 BanII G_rGCy'C Cuts at: 1391 2282 1391 Size: 891 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3992 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3154 3188 1903 Size: 34 1217 34 3678 Fragments arranged by size: 3678 1217 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2855 4065 2855 Size: 1210 3753 BclI T'GATC_A Cuts at: 1548 1548 Size: 4963 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3574 136 Size: 107 122 71 631 605 1902 1525 Fragments arranged by size: 1902 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4958 4958 Size: 4963 BlpI GC'TnA_GC Cuts at: 2380 2380 Size: 4963 Bme1580I G_kGCm'C Cuts at: 1790 3024 4180 1790 Size: 1234 1156 2573 Fragments arranged by size: 2573 1234 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4963 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2694 3652 442 Size: 416 53 83 399 24 1277 958 1753 Fragments arranged by size: 1753 1277 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4963 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3623 1239 Size: 2384 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3088 3953 4251 3088 Size: 865 298 3800 Fragments arranged by size: 3800 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3626 882 Size: 617 2127 2219 Fragments arranged by size: 2219 2127 617 BsaAI yAC'GTr Cuts at: 493 1204 2754 493 Size: 711 1550 2702 Fragments arranged by size: 2702 1550 711 BsaBI GATnn'nnATC Cuts at: 4957 4957 Size: 4963 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2770 3152 3787 275 Size: 53 83 186 936 1237 382 635 1451 Fragments arranged by size: 1451 1237 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3396 3794 4068 4215 4626 3396 Size: 398 274 147 411 3733 Fragments arranged by size: 3733 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4738 Fragments arranged by size: 4738 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4963 BseYI C'CCAG_C Cuts at: 4166 4662 4166 Size: 496 4467 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4390 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2413 3174 4202 1540 Size: 275 186 412 761 1028 2301 Fragments arranged by size: 2301 1028 761 412 275 186 BsiHKAI G_wGCw'C Cuts at: 3024 3109 4180 3024 Size: 85 1071 3807 Fragments arranged by size: 3807 1071 85 BsmI GAATG_Cn' Cuts at: 2474 2567 2474 Size: 93 4870 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2282 3024 3109 4180 1391 Size: 399 492 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 492 399 85 BspEI T'CCGG_A Cuts at: 4626 4626 Size: 4963 BspHI T'CATG_A Cuts at: 2852 4582 2852 Size: 1730 3233 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2318 844 Size: 1474 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2850 1394 Size: 429 1027 3507 Fragments arranged by size: 3507 1027 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3458 3632 65 Size: 1183 2210 174 1396 Fragments arranged by size: 2210 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3607 3794 1700 Size: 110 1797 187 2869 Fragments arranged by size: 2869 1797 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4963 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4963 BssSI C'ACGA_G Cuts at: 3023 4035 3023 Size: 1012 3951 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4963 BstBI TT'CG_AA Cuts at: 3767 3767 Size: 4963 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4963 BstYI r'GATC_y Cuts at: 1972 2293 2660 3045 3062 4514 4600 4612 4958 Size: 321 367 385 17 1452 86 12 346 Cuts at: 4958 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 321 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4411 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4224 Fragments arranged by size: 4224 683 56 BtsI GCAGTG_nn' Cuts at: 2479 3273 3293 2479 Size: 794 20 4149 Fragments arranged by size: 4149 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4963 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4928 DraI TTT'AAA Cuts at: 2267 2267 Size: 4963 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3970 809 Size: 507 2654 1802 Fragments arranged by size: 2654 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2410 3299 4928 Size: 54 1425 50 275 18 580 889 1629 Cuts at: 4928 8 Size: 43 Fragments arranged by size: 1629 1425 889 580 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2410 1537 Size: 275 598 4090 Fragments arranged by size: 4090 598 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2229 2893 1353 Size: 876 664 3423 Fragments arranged by size: 3423 876 664 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3550 3924 4070 387 Size: 1064 138 1961 374 146 1280 Fragments arranged by size: 1961 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3026 3623 4410 4678 1239 Size: 393 1394 597 787 268 1524 Fragments arranged by size: 1524 1394 787 597 393 268 EcoRI G'AATT_C Cuts at: 2272 2650 2272 Size: 378 4585 FspI TGC'GCA Cuts at: 3469 3469 Size: 4963 HaeII r_GCGC'y Cuts at: 1678 4110 1678 Size: 2432 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3604 3636 3678 3710 1373 Size: 32 135 32 2032 32 42 32 2626 Fragments arranged by size: 2626 2032 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2307 2553 669 Size: 988 281 369 246 3079 Fragments arranged by size: 3079 988 369 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4963 HpaI GTT'AAC Cuts at: 2553 2553 Size: 4963 KpnI G_GTAC'C Cuts at: 2288 2288 Size: 4963 MfeI C'AATT_G Cuts at: 2562 2562 Size: 4963 MluI A'CGCG_T Cuts at: 4839 4839 Size: 4963 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4077 4261 1305 Size: 2772 184 2007 Fragments arranged by size: 2772 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3141 Fragments arranged by size: 3141 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2126 2181 2921 3280 3439 518 Size: 648 960 55 740 359 159 2042 Fragments arranged by size: 2042 960 740 648 359 159 55 MspA1I CmG'CkG Cuts at: 1606 2379 3056 4204 4449 1606 Size: 773 677 1148 245 2120 Fragments arranged by size: 2120 1148 773 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4411 NdeI CA'TA_TG Cuts at: 387 387 Size: 4963 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4963 NotI GC'GGCC_GC Cuts at: 2410 2410 Size: 4963 NspI r_CATG'y Cuts at: 1132 1229 2131 2321 3866 1132 Size: 97 902 190 1545 2229 Fragments arranged by size: 2229 1545 902 190 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4963 PciI A'CATG_T Cuts at: 3862 3862 Size: 4963 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4963 PmeI GTTT'AAAC Cuts at: 2267 2267 Size: 4963 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3032 3064 4545 4577 3032 Size: 32 1481 32 3418 Fragments arranged by size: 3418 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4963 PsiI TTA'TAA Cuts at: 2533 2533 Size: 4963 PstI C_TGCA'G Cuts at: 830 2315 830 Size: 1485 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4963 SalI G'TCGA_C Cuts at: 1936 2305 1936 Size: 369 4594 SbfI CC_TGCA'GG Cuts at: 2315 2315 Size: 4963 ScaI AGT'ACT Cuts at: 3211 4923 3211 Size: 1712 3251 SfcI C'TryA_G Cuts at: 826 1045 2109 2311 3446 4127 4318 826 Size: 219 1064 202 1135 681 191 1471 Fragments arranged by size: 1471 1135 1064 681 219 202 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4963 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4963 SmaI CCC'GGG Cuts at: 1872 2290 1872 Size: 418 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2072 3067 3968 4230 820 Size: 197 926 129 995 901 262 1553 Fragments arranged by size: 1553 995 926 901 262 197 129 SnaBI TAC'GTA Cuts at: 493 2754 493 Size: 2261 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4963 SphI G_CATG'C Cuts at: 1132 2321 1132 Size: 1189 3774 SspI AAT'ATT Cuts at: 5 52 2887 5 Size: 47 2835 2081 Fragments arranged by size: 2835 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4963 StyI C'CwwG_G Cuts at: 513 1065 2402 513 Size: 552 1337 3074 Fragments arranged by size: 3074 1337 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2171 2990 3007 3160 3345 3790 325 Size: 1255 591 819 17 153 185 445 1498 Fragments arranged by size: 1498 1255 819 591 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2088 3209 4921 Size: 274 80 33 51 1443 111 1121 1712 Cuts at: 4921 96 Size: 138 Fragments arranged by size: 1712 1443 1121 274 138 111 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2043 2075 4923 4955 2043 Size: 32 2848 32 2051 Fragments arranged by size: 2848 2051 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4963 XbaI T'CTAG_A Cuts at: 2299 2299 Size: 4963 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4963 XmaI C'CCGG_G Cuts at: 1870 2288 1870 Size: 418 4545 XmnI GAAnn'nnTTC Cuts at: 3092 3092 Size: 4963 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2771 276 Size: 53 83 186 936 1237 2468 Fragments arranged by size: 2468 1237 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI