################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HapII HgaI Hin1I HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MaeIII MamI MflI MluI MroI MseI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AfaI 82 GTAC GT^AC AflIII 391 ACATGT A^C[GA][CT]GT AlwNI 280 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 260 AGCGCT AGC^GCT Asp700I 231 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 24 GAGCTC G[AT]GC[AT]^C AvaII 41 GGACC G^G[AT]CC BanI 354 GGTGCC G^G[CT][GA]CC BanII 24 GAGCTC G[GA]GC[CT]^C BfaI 188 CTAG C^TAG BseRI 89 GAGGAG GAGGAG(10/8) BsiHKAI 24 GAGCTC G[AT]GC[AT]^C BsmBI 217 CGTCTC CGTCTC(1/5) BsmFI 40 GGGAC GGGAC(10/14) BspLU11I 391 ACATGT A^CATGT BsrI 498 ACTGG ACTGG(1/-1) DpnII 480 GATC ^GATC DraII 40 GGGACCC [GA]G^G[ACGT]CC[CT] DsaI 240 CCACGG C^C[GA][CT]GG Eco47III 260 AGCGCT AGC^GCT EcoO109I 40 GGGACCC [GA]G^G[ACGT]CC[CT] HaeII 260 AGCGCT [GA]GCGC^[CT] HindIII 460 AAGCTT A^AGCTT HinfI 245 GAGTC G^A[ACGT]TC MaeI 188 CTAG C^TAG MaeII 212 ACGT A^CGT MboI 480 GATC ^GATC MboII 301 GAAGA GAAGA(8/7) MfeI 488 CAATTG C^AATTG MslI 165 CACCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 488 CAATTG C^AATTG NdeII 480 GATC ^GATC NheI 187 GCTAGC G^CTAGC NspI 391 ACATGT [GA]CATG^[CT] PleI 245 GAGTC GAGTC(4/5) PpuMI 40 GGGACCC [GA]G^G[AT]CC[CT] PshAI 211 GACGTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 82 GTAC GT^AC SacI 24 GAGCTC GAGCT^C Sau3AI 480 GATC ^GATC Tsp509I 489 AATT ^AATT XmnI 231 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AciI 5 CCGC CCGC(-3/-1) 162 CCGC BalI 47 TGGCCA TGG^CCA 365 TGGCCA BlpI 372 GCTCAGC GC^T[ACGT]AGC 384 GCTAAGC BmyI 24 GAGCTC G[AGT]GC[ACT]^C 355 GTGCCC Bpu1102I 372 GCTCAGC GC^T[ACGT]AGC 384 GCTAAGC BsaJI 44 CCCTGG C^C[ACGT][ACGT]GG 240 CCACGG BsiYI 63 CCTTCTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 113 CCCACTGCAGG BslI 63 CCTTCTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 113 CCCACTGCAGG BsmAI 218 GTCTC GTCTC(1/5) 516 GTCTC Bsp1286I 24 GAGCTC G[AGT]GC[ACT]^C 355 GTGCCC BspMI 413 ACCTGC ACCTGC(4/8) 279 GCAGGT CelII 372 GCTCAGC GC^T[ACGT]AGC 384 GCTAAGC CfoI 100 GCGC GCG^C 261 GCGC Cfr13I 41 GGACC G^G[ACGT]CC 72 GGCCC HhaI 100 GCGC GCG^C 261 GCGC HinP1I 100 GCGC G^CGC 261 GCGC MluNI 47 TGGCCA TGG^CCA 365 TGGCCA MscI 47 TGGCCA TGG^CCA 365 TGGCCA MspA1I 54 CAGCTG C[AC]G^C[GT]G 95 CAGCTG NlaIII 171 CATG CATG^ 392 CATG PstI 10 CTGCAG CTGCA^G 117 CTGCAG PvuII 54 CAGCTG CAG^CTG 95 CAGCTG Sau96I 41 GGACC G^G[ACGT]CC 72 GGCCC SfcI 10 CTGCAG C^T[GA][CT]AG 117 CTGCAG TspRI 115 CACTG CA[GC]TG[ACGT][ACGT]^ 452 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AluI BbvI BssKI BstNI Cac8I DdeI EaeI EcoRII Fnu4HI HaeIII HphI ItaI MnlI MvaI MwoI NlaIV ScrFI TseI