(Circular) (Six-base) MAPSORT of: pFN21AE1661.seq Check: 3575 from: 1 to: 5242 pFN21AE1661 5242 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2275 2275 Size: 5242 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3052 278 Size: 53 83 186 936 1516 2468 Fragments arranged by size: 2468 1516 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2585 1937 Size: 648 4594 Acc65I G'GTAC_C Cuts at: 2563 2563 Size: 5242 AclI AA'CG_TT Cuts at: 1563 3369 3742 1563 Size: 1806 373 3063 Fragments arranged by size: 3063 1806 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2468 3305 4689 4957 1632 Size: 836 837 1384 268 1917 Fragments arranged by size: 1917 1384 837 836 268 AfeI AGC'GCT Cuts at: 2267 2267 Size: 5242 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5045 AflIII A'CryG_T Cuts at: 1203 2396 4141 5118 1203 Size: 1193 1745 977 1327 Fragments arranged by size: 1745 1327 1193 977 AgeI A'CCGG_T Cuts at: 4073 4073 Size: 5242 AhdI GACnn_n'nnGTC Cuts at: 3971 3971 Size: 5242 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5242 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2192 1184 Size: 1008 4234 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2225 2257 2225 Size: 32 5210 AlwNI CAG_nnn'CTG Cuts at: 2290 2985 4557 2290 Size: 695 1572 2975 Fragments arranged by size: 2975 1572 695 ApaLI G'TGCA_C Cuts at: 3299 4455 3299 Size: 1156 4086 ApoI r'AATT_y Cuts at: 1493 2551 2777 2929 5038 1493 Size: 1058 226 152 2109 1697 Fragments arranged by size: 2109 1697 1058 226 152 AseI AT'TA_AT Cuts at: 160 1032 3796 160 Size: 872 2764 1606 Fragments arranged by size: 2764 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5242 AvaI C'yCGr_G Cuts at: 1870 1943 2567 1870 Size: 73 624 4545 Fragments arranged by size: 4545 624 73 BamHI G'GATC_C Cuts at: 2572 2939 2572 Size: 367 4875 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2359 2563 4018 618 Size: 325 217 627 572 204 1455 1842 Fragments arranged by size: 1842 1455 627 572 325 217 204 BanII G_rGCy'C Cuts at: 1391 2033 2561 1391 Size: 642 528 4072 Fragments arranged by size: 4072 642 528 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4271 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3433 3467 1903 Size: 34 1496 34 3678 Fragments arranged by size: 3678 1496 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3134 4344 3134 Size: 1210 4032 BclI T'GATC_A Cuts at: 1548 1548 Size: 5242 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3853 136 Size: 107 122 71 631 605 2181 1525 Fragments arranged by size: 2181 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5237 5237 Size: 5242 BlpI GC'TnA_GC Cuts at: 2378 2390 2659 2378 Size: 12 269 4961 Fragments arranged by size: 4961 269 12 Bme1580I G_kGCm'C Cuts at: 1790 2364 3303 4459 1790 Size: 574 939 1156 2573 Fragments arranged by size: 2573 1156 939 574 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5242 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2973 3931 442 Size: 416 53 83 399 24 1556 958 1753 Fragments arranged by size: 1753 1556 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2196 1056 Size: 1140 4102 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3902 1239 Size: 2663 2579 Bpu10I CC'TnA_GC Cuts at: 2184 2184 Size: 5242 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3367 4232 4530 3367 Size: 865 298 4079 Fragments arranged by size: 4079 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3905 882 Size: 617 2406 2219 Fragments arranged by size: 2406 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3033 493 Size: 711 1829 2702 Fragments arranged by size: 2702 1829 711 BsaBI GATnn'nnATC Cuts at: 5236 5236 Size: 5242 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3049 3431 4066 275 Size: 53 83 186 936 1516 382 635 1451 Fragments arranged by size: 1516 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3675 4073 4347 4494 4905 3675 Size: 398 274 147 411 4012 Fragments arranged by size: 4012 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2225 2255 1178 Size: 30 165 30 822 30 4165 Fragments arranged by size: 4165 822 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2109 1186 Size: 923 4319 BseYI C'CCAG_C Cuts at: 4445 4941 4445 Size: 496 4746 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4669 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2692 3453 4481 1540 Size: 275 186 691 761 1028 2301 Fragments arranged by size: 2301 1028 761 691 275 186 BsiHKAI G_wGCw'C Cuts at: 2033 3303 3388 4459 2033 Size: 1270 85 1071 2816 Fragments arranged by size: 2816 1270 1071 85 BsmI GAATG_Cn' Cuts at: 2753 2846 2753 Size: 93 5149 BsmBI CGTCTCn'nnnn_ Cuts at: 2228 2228 Size: 5242 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2364 2561 3303 3388 4459 1391 Size: 399 243 331 197 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 331 243 197 85 BspEI T'CCGG_A Cuts at: 4905 4905 Size: 5242 BspHI T'CATG_A Cuts at: 3131 4861 3131 Size: 1730 3512 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2275 2427 2597 844 Size: 1431 152 170 3489 Fragments arranged by size: 3489 1431 170 152 BsrBI CCG'CTC Cuts at: 1394 1823 3129 1394 Size: 429 1306 3507 Fragments arranged by size: 3507 1306 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3737 3911 65 Size: 1183 2489 174 1396 Fragments arranged by size: 2489 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3886 4073 1700 Size: 110 2076 187 2869 Fragments arranged by size: 2869 2076 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5242 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5242 BssSI C'ACGA_G Cuts at: 3302 4314 3302 Size: 1012 4230 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5242 BstBI TT'CG_AA Cuts at: 4046 4046 Size: 5242 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5242 BstYI r'GATC_y Cuts at: 1972 2572 2939 3324 3341 4793 4879 4891 5237 Size: 600 367 385 17 1452 86 12 346 Cuts at: 5237 1972 Size: 1977 Fragments arranged by size: 1977 1452 600 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2245 513 Size: 552 939 241 3510 Fragments arranged by size: 3510 939 552 241 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4503 Fragments arranged by size: 4503 683 56 BtsI GCAGTG_nn' Cuts at: 2119 2758 3552 3572 2119 Size: 639 794 20 3789 Fragments arranged by size: 3789 794 639 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5242 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5207 DraI TTT'AAA Cuts at: 2546 2546 Size: 5242 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4249 809 Size: 507 2933 1802 Fragments arranged by size: 2933 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2052 2370 Size: 54 1425 50 275 18 177 45 318 Cuts at: 2370 2689 3578 5207 8 Size: 319 889 1629 43 Fragments arranged by size: 1629 1425 889 319 318 275 177 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2689 1537 Size: 275 877 4090 Fragments arranged by size: 4090 877 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3172 1353 Size: 1819 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3829 4203 4349 387 Size: 1064 138 2240 374 146 1280 Fragments arranged by size: 2240 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2031 2031 Size: 5242 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2468 3305 3902 4689 4957 1239 Size: 393 836 837 597 787 268 1524 Fragments arranged by size: 1524 837 836 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2046 2046 Size: 5242 EcoRI G'AATT_C Cuts at: 2551 2929 2551 Size: 378 4864 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2158 2158 Size: 5242 FspI TGC'GCA Cuts at: 3748 3748 Size: 5242 HaeII r_GCGC'y Cuts at: 1678 2269 4389 1678 Size: 591 2120 2531 Fragments arranged by size: 2531 2120 591 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3883 3915 3957 3989 1373 Size: 32 135 32 2311 32 42 32 2626 Fragments arranged by size: 2626 2311 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2586 2832 669 Size: 988 281 648 246 3079 Fragments arranged by size: 3079 988 648 281 246 HindIII A'AGCT_T Cuts at: 748 2465 748 Size: 1717 3525 HpaI GTT'AAC Cuts at: 2832 2832 Size: 5242 KpnI G_GTAC'C Cuts at: 2567 2567 Size: 5242 MfeI C'AATT_G Cuts at: 2493 2841 2493 Size: 348 4894 MluI A'CGCG_T Cuts at: 5118 5118 Size: 5242 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2163 4356 4540 1305 Size: 858 2193 184 2007 Fragments arranged by size: 2193 2007 858 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2054 2372 10 Size: 54 1425 343 222 318 2880 Fragments arranged by size: 2880 1425 343 318 222 54 MslI CAynn'nnrTG Cuts at: 518 1166 2174 3200 3559 3718 518 Size: 648 1008 1026 359 159 2042 Fragments arranged by size: 2042 1026 1008 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2061 2102 2658 3335 4483 4728 1606 Size: 455 41 556 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 556 455 245 41 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3751 Fragments arranged by size: 3751 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5242 NheI G'CTAG_C Cuts at: 1052 2192 1052 Size: 1140 4102 NotI GC'GGCC_GC Cuts at: 2689 2689 Size: 5242 NspI r_CATG'y Cuts at: 1132 1229 2400 2600 4145 1132 Size: 97 1171 200 1545 2229 Fragments arranged by size: 2229 1545 1171 200 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5242 PciI A'CATG_T Cuts at: 2396 4141 2396 Size: 1745 3497 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5242 PmeI GTTT'AAAC Cuts at: 2546 2546 Size: 5242 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2225 2257 3311 3343 4824 4856 2225 Size: 32 1054 32 1481 32 2611 Fragments arranged by size: 2611 1481 1054 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2046 2046 Size: 5242 PshAI GACnn'nnGTC Cuts at: 1613 2220 1613 Size: 607 4635 PsiI TTA'TAA Cuts at: 2812 2812 Size: 5242 PstI C_TGCA'G Cuts at: 830 2019 2126 2594 830 Size: 1189 107 468 3478 Fragments arranged by size: 3478 1189 468 107 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5242 PvuII CAG'CTG Cuts at: 2061 2102 2061 Size: 41 5201 SacI G_AGCT'C Cuts at: 2033 2033 Size: 5242 SalI G'TCGA_C Cuts at: 1936 2584 1936 Size: 648 4594 SanDI GG'GwC_CC Cuts at: 2046 2046 Size: 5242 SbfI CC_TGCA'GG Cuts at: 2594 2594 Size: 5242 ScaI AGT'ACT Cuts at: 3490 5202 3490 Size: 1712 3530 SfcI C'TryA_G Cuts at: 826 1045 2015 2122 2590 3725 4406 4597 826 Size: 219 970 107 468 1135 681 191 1471 Fragments arranged by size: 1471 1135 970 681 468 219 191 107 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5242 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5242 SmaI CCC'GGG Cuts at: 1872 2569 1872 Size: 697 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3346 4247 4509 820 Size: 197 926 1403 901 262 1553 Fragments arranged by size: 1553 1403 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3033 493 Size: 2540 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5242 SphI G_CATG'C Cuts at: 1132 2600 1132 Size: 1468 3774 SspI AAT'ATT Cuts at: 5 52 3166 5 Size: 47 3114 2081 Fragments arranged by size: 3114 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5242 StyI C'CwwG_G Cuts at: 513 1065 2004 2681 513 Size: 552 939 677 3074 Fragments arranged by size: 3074 939 677 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3269 3286 3439 3624 4069 325 Size: 1255 1689 17 153 185 445 1498 Fragments arranged by size: 1689 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3488 5200 96 Size: 274 80 33 51 1443 1511 1712 138 Fragments arranged by size: 1712 1511 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5202 5234 5202 Size: 32 5210 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5242 XbaI T'CTAG_A Cuts at: 2578 2578 Size: 5242 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5242 XmaI C'CCGG_G Cuts at: 1870 2567 1870 Size: 697 4545 XmnI GAAnn'nnTTC Cuts at: 2240 3371 2240 Size: 1131 4111 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3050 276 Size: 53 83 186 936 1516 2468 Fragments arranged by size: 2468 1516 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI