Restriction map for pFN21AE1661
 (Circular) (Six-base) MAPSORT of: pFN21AE1661.seq  Check: 3575  from: 1  to: 5242



pFN21AE1661  5242 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:52  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2275    2275 
   Size:       5242
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3052     278 
   Size:         53      83     186     936    1516    2468

  Fragments arranged by size: 

                2468    1516     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2585    1937 
   Size:        648    4594
 
Acc65I G'GTAC_C

Cuts at:   2563    2563 
   Size:       5242
 
AclI AA'CG_TT

Cuts at:   1563    3369    3742    1563 
   Size:       1806     373    3063

  Fragments arranged by size: 

                3063    1806     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2468    3305    4689    4957    1632 
   Size:        836     837    1384     268    1917

  Fragments arranged by size: 

                1917    1384     837     836     268
 
AfeI AGC'GCT

Cuts at:   2267    2267 
   Size:       5242
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5045
 
AflIII A'CryG_T

Cuts at:   1203    2396    4141    5118    1203 
   Size:       1193    1745     977    1327

  Fragments arranged by size: 

                1745    1327    1193     977
 
AgeI A'CCGG_T

Cuts at:   4073    4073 
   Size:       5242
 
AhdI GACnn_n'nnGTC

Cuts at:   3971    3971 
   Size:       5242
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5242
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2192    1184 
   Size:       1008    4234
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2225    2257    2225 
   Size:         32    5210
 
AlwNI CAG_nnn'CTG

Cuts at:   2290    2985    4557    2290 
   Size:        695    1572    2975

  Fragments arranged by size: 

                2975    1572     695
 
ApaLI G'TGCA_C

Cuts at:   3299    4455    3299 
   Size:       1156    4086
 
ApoI r'AATT_y

Cuts at:   1493    2551    2777    2929    5038    1493 
   Size:       1058     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1058     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3796     160 
   Size:        872    2764    1606

  Fragments arranged by size: 

                2764    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5242
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2567    1870 
   Size:         73     624    4545

  Fragments arranged by size: 

                4545     624      73
 
BamHI G'GATC_C

Cuts at:   2572    2939    2572 
   Size:        367    4875
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2359    2563    4018     618 
   Size:        325     217     627     572     204    1455    1842

  Fragments arranged by size: 

                1842    1455     627     572     325     217     204
 
BanII G_rGCy'C

Cuts at:   1391    2033    2561    1391 
   Size:        642     528    4072

  Fragments arranged by size: 

                4072     642     528
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4271
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3433    3467    1903 
   Size:         34    1496      34    3678

  Fragments arranged by size: 

                3678    1496      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3134    4344    3134 
   Size:       1210    4032
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5242
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3853     136 
   Size:        107     122      71     631     605    2181    1525

  Fragments arranged by size: 

                2181    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5237    5237 
   Size:       5242
 
BlpI GC'TnA_GC

Cuts at:   2378    2390    2659    2378 
   Size:         12     269    4961

  Fragments arranged by size: 

                4961     269      12
 
Bme1580I G_kGCm'C

Cuts at:   1790    2364    3303    4459    1790 
   Size:        574     939    1156    2573

  Fragments arranged by size: 

                2573    1156     939     574
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5242
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2973    3931     442 
   Size:        416      53      83     399      24    1556     958    1753

  Fragments arranged by size: 

                1753    1556     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    2196    1056 
   Size:       1140    4102
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3902    1239 
   Size:       2663    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2184    2184 
   Size:       5242
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3367    4232    4530    3367 
   Size:        865     298    4079

  Fragments arranged by size: 

                4079     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3905     882 
   Size:        617    2406    2219

  Fragments arranged by size: 

                2406    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3033     493 
   Size:        711    1829    2702

  Fragments arranged by size: 

                2702    1829     711
 
BsaBI GATnn'nnATC

Cuts at:   5236    5236 
   Size:       5242
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3049    3431    4066     275 
   Size:         53      83     186     936    1516     382     635    1451

  Fragments arranged by size: 

                1516    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3675    4073    4347    4494    4905    3675 
   Size:        398     274     147     411    4012

  Fragments arranged by size: 

                4012     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2225    2255    1178 
   Size:         30     165      30     822      30    4165

  Fragments arranged by size: 

                4165     822     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2109    1186 
   Size:        923    4319
 
BseYI C'CCAG_C

Cuts at:   4445    4941    4445 
   Size:        496    4746
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4669
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2692    3453    4481    1540 
   Size:        275     186     691     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     691     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2033    3303    3388    4459    2033 
   Size:       1270      85    1071    2816

  Fragments arranged by size: 

                2816    1270    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2753    2846    2753 
   Size:         93    5149
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2228    2228 
   Size:       5242
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2033    2364    2561    3303    3388    4459    1391 
   Size:        399     243     331     197     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     331     243     197      85
            
 
BspEI T'CCGG_A

Cuts at:   4905    4905 
   Size:       5242
 
BspHI T'CATG_A

Cuts at:   3131    4861    3131 
   Size:       1730    3512
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2275    2427    2597     844 
   Size:       1431     152     170    3489

  Fragments arranged by size: 

                3489    1431     170     152
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3129    1394 
   Size:        429    1306    3507

  Fragments arranged by size: 

                3507    1306     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3737    3911      65 
   Size:       1183    2489     174    1396

  Fragments arranged by size: 

                2489    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3886    4073    1700 
   Size:        110    2076     187    2869

  Fragments arranged by size: 

                2869    2076     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5242
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5242
 
BssSI C'ACGA_G

Cuts at:   3302    4314    3302 
   Size:       1012    4230
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5242
 
BstBI TT'CG_AA

Cuts at:   4046    4046 
   Size:       5242
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5242
 
BstYI r'GATC_y

Cuts at:   1972    2572    2939    3324    3341    4793    4879    4891    5237 
   Size:        600     367     385      17    1452      86      12     346

Cuts at:   5237    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     600     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2245     513 
   Size:        552     939     241    3510

  Fragments arranged by size: 

                3510     939     552     241
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4503

  Fragments arranged by size: 

                4503     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2119    2758    3552    3572    2119 
   Size:        639     794      20    3789

  Fragments arranged by size: 

                3789     794     639      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5242
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5207
 
DraI TTT'AAA

Cuts at:   2546    2546 
   Size:       5242
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4249     809 
   Size:        507    2933    1802

  Fragments arranged by size: 

                2933    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2007    2052    2370 
   Size:         54    1425      50     275      18     177      45     318

Cuts at:   2370    2689    3578    5207       8 
   Size:        319     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     319     318     275     177      54
                  50      45      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2689    1537 
   Size:        275     877    4090

  Fragments arranged by size: 

                4090     877     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3172    1353 
   Size:       1819    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3829    4203    4349     387 
   Size:       1064     138    2240     374     146    1280

  Fragments arranged by size: 

                2240    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2031    2031 
   Size:       5242
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2468    3305    3902    4689    4957    1239 
   Size:        393     836     837     597     787     268    1524

  Fragments arranged by size: 

                1524     837     836     787     597     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2046    2046 
   Size:       5242
 
EcoRI G'AATT_C

Cuts at:   2551    2929    2551 
   Size:        378    4864
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2158    2158 
   Size:       5242
 
FspI TGC'GCA

Cuts at:   3748    3748 
   Size:       5242
 
HaeII r_GCGC'y

Cuts at:   1678    2269    4389    1678 
   Size:        591    2120    2531

  Fragments arranged by size: 

                2531    2120     591
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3883    3915    3957    3989    1373 
   Size:         32     135      32    2311      32      42      32    2626

  Fragments arranged by size: 

                2626    2311     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2586    2832     669 
   Size:        988     281     648     246    3079

  Fragments arranged by size: 

                3079     988     648     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2465     748 
   Size:       1717    3525
 
HpaI GTT'AAC

Cuts at:   2832    2832 
   Size:       5242
 
KpnI G_GTAC'C

Cuts at:   2567    2567 
   Size:       5242
 
MfeI C'AATT_G

Cuts at:   2493    2841    2493 
   Size:        348    4894
 
MluI A'CGCG_T

Cuts at:   5118    5118 
   Size:       5242
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2163    4356    4540    1305 
   Size:        858    2193     184    2007

  Fragments arranged by size: 

                2193    2007     858     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2054    2372      10 
   Size:         54    1425     343     222     318    2880

  Fragments arranged by size: 

                2880    1425     343     318     222      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2174    3200    3559    3718     518 
   Size:        648    1008    1026     359     159    2042

  Fragments arranged by size: 

                2042    1026    1008     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2061    2102    2658    3335    4483    4728    1606 
   Size:        455      41     556     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     556     455     245      41
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3751

  Fragments arranged by size: 

                3751     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5242
 
NheI G'CTAG_C

Cuts at:   1052    2192    1052 
   Size:       1140    4102
 
NotI GC'GGCC_GC

Cuts at:   2689    2689 
   Size:       5242
 
NspI r_CATG'y

Cuts at:   1132    1229    2400    2600    4145    1132 
   Size:         97    1171     200    1545    2229

  Fragments arranged by size: 

                2229    1545    1171     200      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5242
 
PciI A'CATG_T

Cuts at:   2396    4141    2396 
   Size:       1745    3497
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5242
 
PmeI GTTT'AAAC

Cuts at:   2546    2546 
   Size:       5242
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2225    2257    3311    3343    4824    4856    2225 
   Size:         32    1054      32    1481      32    2611

  Fragments arranged by size: 

                2611    1481    1054      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2046    2046 
   Size:       5242
 
PshAI GACnn'nnGTC

Cuts at:   1613    2220    1613 
   Size:        607    4635
 
PsiI TTA'TAA

Cuts at:   2812    2812 
   Size:       5242
 
PstI C_TGCA'G

Cuts at:    830    2019    2126    2594     830 
   Size:       1189     107     468    3478

  Fragments arranged by size: 

                3478    1189     468     107
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5242
 
PvuII CAG'CTG

Cuts at:   2061    2102    2061 
   Size:         41    5201
 
SacI G_AGCT'C

Cuts at:   2033    2033 
   Size:       5242
 
SalI G'TCGA_C

Cuts at:   1936    2584    1936 
   Size:        648    4594
 
SanDI GG'GwC_CC

Cuts at:   2046    2046 
   Size:       5242
 
SbfI CC_TGCA'GG

Cuts at:   2594    2594 
   Size:       5242
 
ScaI AGT'ACT

Cuts at:   3490    5202    3490 
   Size:       1712    3530
 
SfcI C'TryA_G

Cuts at:    826    1045    2015    2122    2590    3725    4406    4597     826 
   Size:        219     970     107     468    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135     970     681     468     219     191     107
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5242
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5242
 
SmaI CCC'GGG

Cuts at:   1872    2569    1872 
   Size:        697    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3346    4247    4509     820 
   Size:        197     926    1403     901     262    1553

  Fragments arranged by size: 

                1553    1403     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3033     493 
   Size:       2540    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5242
 
SphI G_CATG'C

Cuts at:   1132    2600    1132 
   Size:       1468    3774
 
SspI AAT'ATT

Cuts at:      5      52    3166       5 
   Size:         47    3114    2081

  Fragments arranged by size: 

                3114    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5242
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2681     513 
   Size:        552     939     677    3074

  Fragments arranged by size: 

                3074     939     677     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3269    3286    3439    3624    4069     325 
   Size:       1255    1689      17     153     185     445    1498

  Fragments arranged by size: 

                1689    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3488    5200      96 
   Size:        274      80      33      51    1443    1511    1712     138

  Fragments arranged by size: 

                1712    1511    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5202    5234    5202 
   Size:         32    5210
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5242
 
XbaI T'CTAG_A

Cuts at:   2578    2578 
   Size:       5242
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5242
 
XmaI C'CCGG_G

Cuts at:   1870    2567    1870 
   Size:        697    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2240    3371    2240 
   Size:       1131    4111
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3050     276 
   Size:         53      83     186     936    1516    2468

  Fragments arranged by size: 

                2468    1516     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI
     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI    PpuMI
    PshAI     PsiI     PstI     PvuI    PvuII     SacI     SalI    SanDI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI    BfrBI     BplI
    BsiWI    BstXI  BstZ17I   Bsu36I   DraIII    EcoNI    EcoRV     FseI
    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI
     PfoI     PmlI   PspOMI    PspXI     PsrI    RsrII    SacII     SapI
    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com