################################## # NO CUT ENZYMES ( 197 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaJI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeII NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TfiI TspRI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AciI 136 GCGG CCGC(-3/-1) AflII 100 CTTAAG C^TTAAG AlwNI 466 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 72 GGACC G^G[AT]CC BbsI 292 GAAGAC GAAGAC(2/6) BbvI 436 GCAGC GCAGC(8/12) BfrI 100 CTTAAG C^TTAAG BpuAI 292 GAAGAC GAAGAC(2/6) BsaI 280 GAGACC GGTCTC(1/5) BseRI 181 GAGGAG GAGGAG(10/8) BsiYI 411 CCAAGTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 411 CCAAGTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspMI 118 GCAGGT ACCTGC(4/8) BssKI 69 CCAGG ^CC[ACGT]GG BstBI 343 TTCGAA TT^CGAA BstNI 69 CCAGG CC^[AT]GG Cac8I 432 GCCAGC GC[ACGT]^[ACGT]GC Cfr13I 72 GGACC G^G[ACGT]CC DdeI 156 CTTAG C^T[ACGT]AG DraII 71 AGGACCC [GA]G^G[ACGT]CC[CT] EarI 485 GAAGAG CTCTTC(1/4) EcoO109I 71 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRII 69 CCAGG ^CC[AT]GG Fnu4HI 436 GCAGC GC^[ACGT]GC HinfI 462 GAGTC G^A[ACGT]TC ItaI 436 GCAGC GC^[ACGT]GC Ksp632I 485 GAAGAG CTCTTC(1/4) MvaI 69 CCAGG CC^[AT]GG MwoI 59 GCTGTTACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 77 CATATG CA^TATG NlaIV 72 GGACCC GG[ACGT]^[ACGT]CC NspV 343 TTCGAA TT^CGAA PflMI 411 CCAAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 462 GAGTC GAGTC(4/5) PpuMI 71 AGGACCC [GA]G^G[AT]CC[CT] SapI 485 GAAGAGC GCTCTTC(1/4) Sau96I 72 GGACC G^G[ACGT]CC ScrFI 69 CCAGG CC^[ACGT]GG SfuI 343 TTCGAA TT^CGAA SmlI 100 CTTAAG C^T[CT][GA]AG SspI 107 AATATT AAT^ATT TseI 436 GCAGC G^C[AT]GC Tsp45I 188 GTGAC ^GT[GC]AC Van91I 411 CCAAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 6 enzymes) # ################################### BsmAI 280 GAGAC GTCTC(1/5) 217 GTCTC DraI 238 TTTAAA TTT^AAA 247 TTTAAA DrdI 295 GACATGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 373 GACTTGAATGTC MnlI 181 GAGG CCTC(7/6) 479 GAGG MslI 124 CATTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 408 CATCCAAGTG NlaIII 297 CATG CATG^ 400 CATG ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AluI BstF5I FokI MaeIII MboII MseI SfaNI TaqI Tru9I Tsp509I TthHB8I