################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII AccII AclI AcsI AflIII AgeI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AviII AvrII BalI BamHI BbvCI BcgI BclI BglI BglII BlnI BsaBI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI Ksp632I KspI MamI MfeI MluI MluNI MscI MslI MunI MvnI NaeI NcoI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SmaI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 809 GGTACC G^GTACC AccI 760 GTAGAC GT^[AC][GT]AC AccIII 227 TCCGGA T^CCGGA AcyI 449 GGCGCC G[GA]^CG[CT]C AflII 661 CTTAAG C^TTAAG AhdI 171 GACCAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 210 AGCGCT AGC^GCT Asp718I 809 GGTACC G^GTACC AspEI 171 GACCAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 63 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 802 CCCGAG C^[CT]CG[GA]G BbeI 449 GGCGCC GGCGC^C BbrPI 385 CACGTG CAC^GTG BbsI 892 GTCTTC GAAGAC(2/6) BciVI 791 GGATAC GTATCC(6/5) BcnI 693 CCGGG CC^[GC]GG BfrI 661 CTTAAG C^TTAAG BlpI 475 GCTGAGC GC^T[ACGT]AGC Bpu1102I 475 GCTGAGC GC^T[ACGT]AGC BpuAI 892 GTCTTC GAAGAC(2/6) BsaHI 449 GGCGCC G[GA]^CG[CT]C BsaI 749 GAGACC GGTCTC(1/5) BsaWI 227 TCCGGA [AT]^CCGG[AT] BseAI 227 TCCGGA T^CCGGA BsgI 382 CTGCAC GTGCAG(16/14) BsoBI 802 CCCGAG C^[CT]CG[GA]G BspEI 227 TCCGGA T^CCGGA BstAPI 407 GCACTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 846 CCACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 475 GCTGAGC GC^T[ACGT]AGC DdeI 476 CTGAG C^T[ACGT]AG Eam1105I 171 GACCAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 210 AGCGCT AGC^GCT EcoNI 682 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Hin1I 449 GGCGCC G[GA]^CG[CT]C HinfI 298 GACTC G^A[ACGT]TC KasI 449 GGCGCC G^GCGCC KpnI 809 GGTACC GGTAC^C MroI 227 TCCGGA T^CCGGA MspA1I 814 CAGCTG C[AC]G^C[GT]G NarI 449 GGCGCC GG^CGCC NciI 693 CCGGG CC^[GC]GG NheI 135 GCTAGC G^CTAGC NlaIII 367 CATG CATG^ PflFI 63 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 298 GACTC GAGTC(4/5) PmaCI 385 CACGTG CAC^GTG PmlI 385 CACGTG CAC^GTG PvuII 814 CAGCTG CAG^CTG SacI 119 GAGCTC GAGCT^C SfoI 449 GGCGCC GGC^GCC SnaBI 757 TACGTA TAC^GTA StuI 1034 AGGCCT AGG^CCT Tsp45I 292 GTCAC ^GT[GC]AC Tsp509I 820 AATT ^AATT Tth111I 63 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BsaAI 385 CACGTG [CT]AC^GT[GA] 757 TACGTA BsmAI 231 GAGAC GTCTC(1/5) 749 GAGAC BsrDI 867 CATTGC GCAATG(2/0) 995 GCAATG BstYI 422 GGATCT [GA]^GATC[CT] 665 AGATCC CfoI 211 GCGC GCG^C 450 GCGC HaeII 210 AGCGCT [GA]GCGC^[CT] 449 GGCGCC HapII 228 CCGG C^CGG 693 CCGG HhaI 211 GCGC GCG^C 450 GCGC HinP1I 211 GCGC G^CGC 450 GCGC HpaII 228 CCGG C^CGG 693 CCGG MaeII 386 ACGT A^CGT 758 ACGT MaeIII 292 GTCAC ^GT[ACGT]AC 601 GTTAC MboII 425 TCTTC GAAGA(8/7) 893 TCTTC MflI 422 GGATCT [GA]^GATC[CT] 665 AGATCC MseI 511 TTAA T^TAA 662 TTAA MspI 228 CCGG C^CGG 693 CCGG PflMI 256 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 768 CCAGGAACTGG PpuMI 457 GGGTCCC [GA]G^G[AT]CC[CT] 706 AGGACCC Tru9I 511 TTAA T^TAA 662 TTAA Van91I 256 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 768 CCAGGAACTGG XhoII 422 GGATCT [GA]^GATC[CT] 665 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaII BanI BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HphI ItaI MaeI MboI MnlI MvaI MwoI NdeII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfcI SmlI StyI TseI TspRI