(Circular) (Six-base) MAPSORT of: pFN21AE5019.seq Check: 2750 from: 1 to: 5755 pFN21AE5019 5755 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3565 278 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2766 3098 1937 Size: 829 332 4594 Fragments arranged by size: 4594 829 332 Acc65I G'GTAC_C Cuts at: 2814 3076 2814 Size: 262 5493 AclI AA'CG_TT Cuts at: 1563 3882 4255 1563 Size: 2319 373 3063 Fragments arranged by size: 3063 2319 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2295 2884 3818 5202 5470 1632 Size: 663 589 934 1384 268 1917 Fragments arranged by size: 1917 1384 934 663 589 268 AfeI AGC'GCT Cuts at: 2217 2217 Size: 5755 AflII C'TTAA_G Cuts at: 820 1017 2666 820 Size: 197 1649 3909 Fragments arranged by size: 3909 1649 197 AflIII A'CryG_T Cuts at: 1203 4654 5631 1203 Size: 3451 977 1327 Fragments arranged by size: 3451 1327 977 AgeI A'CCGG_T Cuts at: 4586 4586 Size: 5755 AhdI GACnn_n'nnGTC Cuts at: 2181 4484 2181 Size: 2303 3452 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5755 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5755 AlwNI CAG_nnn'CTG Cuts at: 2075 2609 2779 3498 5070 2075 Size: 534 170 719 1572 2760 Fragments arranged by size: 2760 1572 719 534 170 ApaLI G'TGCA_C Cuts at: 3812 4968 3812 Size: 1156 4599 ApoI r'AATT_y Cuts at: 1493 3064 3290 3442 5551 1493 Size: 1571 226 152 2109 1697 Fragments arranged by size: 2109 1697 1571 226 152 AseI AT'TA_AT Cuts at: 160 1032 4309 160 Size: 872 3277 1606 Fragments arranged by size: 3277 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 2807 3080 1870 Size: 73 864 273 4545 Fragments arranged by size: 4545 864 273 73 BamHI G'GATC_C Cuts at: 3085 3452 3085 Size: 367 5388 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2107 2454 2814 3076 4531 Size: 325 217 627 320 347 360 262 1455 Cuts at: 4531 618 Size: 1842 Fragments arranged by size: 1842 1455 627 360 347 325 320 262 217 BanII G_rGCy'C Cuts at: 1391 2099 2128 2230 3074 1391 Size: 708 29 102 844 4072 Fragments arranged by size: 4072 844 708 102 29 BbeI G_GCGC'C Cuts at: 2458 2458 Size: 5755 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2890 928 Size: 971 991 3793 Fragments arranged by size: 3793 991 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3946 3980 1903 Size: 34 50 34 1925 34 3678 Fragments arranged by size: 3678 1925 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2790 3647 4857 2790 Size: 857 1210 3688 Fragments arranged by size: 3688 1210 857 BclI T'GATC_A Cuts at: 1548 1548 Size: 5755 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4366 136 Size: 107 122 71 631 605 2694 1525 Fragments arranged by size: 2694 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5750 5750 Size: 5755 BlpI GC'TnA_GC Cuts at: 2481 3172 2481 Size: 691 5064 Bme1580I G_kGCm'C Cuts at: 1790 2050 2846 3816 4972 1790 Size: 260 796 970 1156 2573 Fragments arranged by size: 2573 1156 970 796 260 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5755 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2789 3486 4444 Size: 416 53 83 399 24 1372 697 958 Cuts at: 4444 442 Size: 1753 Fragments arranged by size: 1753 1372 958 697 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2144 1056 Size: 1088 4667 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2274 2274 Size: 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2244 2709 4415 1239 Size: 917 88 465 1706 2579 Fragments arranged by size: 2579 1706 917 465 88 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2443 2557 3880 4745 5043 2443 Size: 114 1323 865 298 3155 Fragments arranged by size: 3155 1323 865 298 114 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2748 4418 882 Size: 617 1249 1670 2219 Fragments arranged by size: 2219 1670 1249 617 BsaAI yAC'GTr Cuts at: 493 1204 2392 2764 3546 493 Size: 711 1188 372 782 2702 Fragments arranged by size: 2702 1188 782 711 372 BsaBI GATnn'nnATC Cuts at: 5749 5749 Size: 5755 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2455 3562 3944 4579 Size: 53 83 186 936 922 1107 382 635 Cuts at: 4579 275 Size: 1451 Fragments arranged by size: 1451 1107 936 922 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2232 4188 4586 4860 5007 5418 2232 Size: 1956 398 274 147 411 2569 Fragments arranged by size: 2569 1956 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2185 2215 1178 Size: 30 165 30 782 30 4718 Fragments arranged by size: 4718 782 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2288 2340 2464 2722 2897 2969 1186 Size: 1102 52 124 258 175 72 3972 Fragments arranged by size: 3972 1102 258 175 124 72 52 BseYI C'CCAG_C Cuts at: 4958 5454 4958 Size: 496 5259 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2372 1163 Size: 573 636 4546 Fragments arranged by size: 4546 636 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3205 3966 4994 1540 Size: 275 186 1204 761 1028 2301 Fragments arranged by size: 2301 1204 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2062 2128 2397 2487 3816 3901 4972 2062 Size: 66 269 90 1329 85 1071 2845 Fragments arranged by size: 2845 1329 1071 269 90 85 66 BsmI GAATG_Cn' Cuts at: 3266 3359 3266 Size: 93 5662 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2050 2062 2099 2128 2230 2397 2487 Size: 399 260 12 37 29 102 167 90 Cuts at: 2487 2846 3074 3816 3901 4972 1391 Size: 359 228 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 359 260 228 167 102 90 85 37 29 12 BspEI T'CCGG_A Cuts at: 2232 5418 2232 Size: 3186 2569 BspHI T'CATG_A Cuts at: 3644 5374 3644 Size: 1730 4025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3110 844 Size: 2266 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3642 1394 Size: 429 1819 3507 Fragments arranged by size: 3507 1819 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2871 3007 4250 4424 65 Size: 1183 1623 136 1243 174 1396 Fragments arranged by size: 1623 1396 1243 1183 174 136 BsrFI r'CCGG_y Cuts at: 1700 1810 4399 4586 1700 Size: 110 2589 187 2869 Fragments arranged by size: 2869 2589 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5755 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5755 BssSI C'ACGA_G Cuts at: 3815 4827 3815 Size: 1012 4743 BstAPI GCAn_nnn'nTGC Cuts at: 794 2418 794 Size: 1624 4131 BstBI TT'CG_AA Cuts at: 4559 4559 Size: 5755 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5755 BstXI CCAn_nnnn'nTGG Cuts at: 2858 2858 Size: 5755 BstYI r'GATC_y Cuts at: 1972 2427 2670 3085 3452 3837 3854 5306 5392 Size: 455 243 415 367 385 17 1452 86 Cuts at: 5392 5404 5750 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 455 415 385 367 346 243 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5203 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5016 Fragments arranged by size: 5016 683 56 BtsI GCAGTG_nn' Cuts at: 2839 2846 3271 4065 4085 2839 Size: 7 425 794 20 4509 Fragments arranged by size: 4509 794 425 20 7 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5755 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5720 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4762 809 Size: 507 3446 1802 Fragments arranged by size: 3446 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3202 4091 5720 Size: 54 1425 50 275 18 1372 889 1629 Cuts at: 5720 8 Size: 43 Fragments arranged by size: 1629 1425 1372 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3202 1537 Size: 275 1390 4090 Fragments arranged by size: 4090 1390 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3685 1353 Size: 2332 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4342 4716 4862 387 Size: 1064 138 2753 374 146 1280 Fragments arranged by size: 2753 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2126 2126 Size: 5755 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2244 2295 2709 2884 3818 4415 Size: 393 524 88 51 414 175 934 597 Cuts at: 4415 5202 5470 1239 Size: 787 268 1524 Fragments arranged by size: 1524 934 787 597 524 414 393 268 175 88 51 EcoNI CCTnn'n_nnAGG Cuts at: 2691 2691 Size: 5755 EcoO109I rG'GnC_Cy Cuts at: 2463 2712 2967 2463 Size: 249 255 5251 Fragments arranged by size: 5251 255 249 EcoRI G'AATT_C Cuts at: 3064 3442 3064 Size: 378 5377 FspI TGC'GCA Cuts at: 4261 4261 Size: 5755 HaeII r_GCGC'y Cuts at: 1678 2219 2458 4902 1678 Size: 541 239 2444 2531 Fragments arranged by size: 2531 2444 541 239 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2167 2199 4396 4428 4470 Size: 32 135 32 595 32 2197 32 42 Cuts at: 4470 4502 1373 Size: 32 2626 Fragments arranged by size: 2626 2197 595 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3099 3345 669 Size: 988 281 1161 246 3079 Fragments arranged by size: 3079 1161 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 3345 3345 Size: 5755 KasI G'GCGC_C Cuts at: 2454 2454 Size: 5755 KpnI G_GTAC'C Cuts at: 2818 3080 2818 Size: 262 5493 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4869 5053 1305 Size: 3564 184 2007 Fragments arranged by size: 3564 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3933 Fragments arranged by size: 3933 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3713 4072 4231 518 Size: 648 2547 359 159 2042 Fragments arranged by size: 2547 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2821 3171 3848 4996 5241 1606 Size: 1215 350 677 1148 245 2120 Fragments arranged by size: 2120 1215 1148 677 350 245 NarI GG'CG_CC Cuts at: 2455 2455 Size: 5755 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5203 NdeI CA'TA_TG Cuts at: 387 387 Size: 5755 NheI G'CTAG_C Cuts at: 1052 2140 1052 Size: 1088 4667 NotI GC'GGCC_GC Cuts at: 3202 3202 Size: 5755 NspI r_CATG'y Cuts at: 1132 1229 3113 4658 1132 Size: 97 1884 1545 2229 Fragments arranged by size: 2229 1884 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5755 PciI A'CATG_T Cuts at: 4654 4654 Size: 5755 PflMI CCAn_nnn'nTGG Cuts at: 1108 2267 2779 1108 Size: 1159 512 4084 Fragments arranged by size: 4084 1159 512 PfoI T'CCnGG_A Cuts at: 2157 2772 2157 Size: 615 5140 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PmlI CAC'GTG Cuts at: 2392 2392 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3824 3856 5337 5369 3824 Size: 32 1481 32 4210 Fragments arranged by size: 4210 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2463 2712 2463 Size: 249 5506 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5755 PsiI TTA'TAA Cuts at: 3325 3325 Size: 5755 PstI C_TGCA'G Cuts at: 830 2582 2805 2834 3053 3107 830 Size: 1752 223 29 219 54 3478 Fragments arranged by size: 3478 1752 223 219 54 29 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 PvuII CAG'CTG Cuts at: 2821 2821 Size: 5755 SacI G_AGCT'C Cuts at: 2128 2128 Size: 5755 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SanDI GG'GwC_CC Cuts at: 2463 2463 Size: 5755 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 4003 5715 4003 Size: 1712 4043 SfcI C'TryA_G Cuts at: 826 1045 2240 2317 2578 2801 2830 3049 3103 Size: 219 1195 77 261 223 29 219 54 Cuts at: 3103 4238 4919 5110 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1195 1135 681 261 223 219 219 191 77 54 29 SfoI GGC'GCC Cuts at: 2456 2456 Size: 5755 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5755 SmaI CCC'GGG Cuts at: 1872 3082 1872 Size: 1210 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2422 2536 2666 3859 4760 5022 Size: 197 926 479 114 130 1193 901 262 Cuts at: 5022 820 Size: 1553 Fragments arranged by size: 1553 1193 926 901 479 262 197 130 114 SnaBI TAC'GTA Cuts at: 493 2764 3546 493 Size: 2271 782 2702 Fragments arranged by size: 2702 2271 782 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 3113 1132 Size: 1981 3774 SspI AAT'ATT Cuts at: 5 52 3679 5 Size: 47 3627 2081 Fragments arranged by size: 3627 2081 47 StuI AGG'CCT Cuts at: 1517 3041 1517 Size: 1524 4231 StyI C'CwwG_G Cuts at: 513 1065 2444 2458 2924 3194 513 Size: 552 1379 14 466 270 3074 Fragments arranged by size: 3074 1379 552 466 270 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2343 3782 3799 3952 4137 4582 325 Size: 1255 763 1439 17 153 185 445 1498 Fragments arranged by size: 1498 1439 1255 763 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4001 5713 96 Size: 274 80 33 51 1443 2024 1712 138 Fragments arranged by size: 2024 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5715 5747 5715 Size: 32 5723 Tth111I GACn'n_nGTC Cuts at: 1316 2071 1316 Size: 755 5000 XbaI T'CTAG_A Cuts at: 3091 3091 Size: 5755 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5755 XmaI C'CCGG_G Cuts at: 1870 3080 1870 Size: 1210 4545 XmnI GAAnn'nnTTC Cuts at: 3884 3884 Size: 5755 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3563 276 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI