(Linear) (Six-base) MAPSORT of: KIEE5019.seq Check: 163 from: 1 to: 1050 KIEE5019.orf With 182 enzymes: SgfI * December 5, 2014 12:58 .. AccI GT'mk_AC Cuts at: 0 761 1050 Size: 761 289 Acc65I G'GTAC_C Cuts at: 0 809 1050 Size: 809 241 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 290 879 1050 Size: 290 589 171 Fragments arranged by size: 589 290 171 AfeI AGC'GCT Cuts at: 0 212 1050 Size: 212 838 AflII C'TTAA_G Cuts at: 0 661 1050 Size: 661 389 AhdI GACnn_n'nnGTC Cuts at: 0 176 1050 Size: 176 874 AlwNI CAG_nnn'CTG Cuts at: 0 70 604 774 1050 Size: 70 534 170 276 Fragments arranged by size: 534 276 170 70 AvaI C'yCGr_G Cuts at: 0 802 1050 Size: 802 248 BanI G'GyrC_C Cuts at: 0 102 449 809 1050 Size: 102 347 360 241 Fragments arranged by size: 360 347 241 102 BanII G_rGCy'C Cuts at: 0 94 123 225 1050 Size: 94 29 102 825 Fragments arranged by size: 825 102 94 29 BbeI G_GCGC'C Cuts at: 0 453 1050 Size: 453 597 BbsI GAAGACnn'nnnn_ Cuts at: 0 885 1050 Size: 885 165 BciVI GTATCCnnnnn_n' Cuts at: 0 785 1050 Size: 785 265 BlpI GC'TnA_GC Cuts at: 0 476 1050 Size: 476 574 Bme1580I G_kGCm'C Cuts at: 0 45 841 1050 Size: 45 796 209 Fragments arranged by size: 796 209 45 BmrI ACTGGGnnnn_n' Cuts at: 0 784 1050 Size: 784 266 BmtI G_CTAG'C Cuts at: 0 139 1050 Size: 139 911 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 269 1050 Size: 269 781 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 239 704 1050 Size: 151 88 465 346 Fragments arranged by size: 465 346 151 88 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 438 552 1050 Size: 438 114 498 Fragments arranged by size: 498 438 114 BsaI GGTCTCn'nnnn_ Cuts at: 0 743 1050 Size: 743 307 BsaAI yAC'GTr Cuts at: 0 387 759 1050 Size: 387 372 291 Fragments arranged by size: 387 372 291 BsaHI Gr'CG_yC Cuts at: 0 450 1050 Size: 450 600 BsaWI w'CCGG_w Cuts at: 0 227 1050 Size: 227 823 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 180 210 1050 Size: 180 30 840 Fragments arranged by size: 840 180 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 283 335 459 717 892 964 1050 Size: 283 52 124 258 175 72 86 Fragments arranged by size: 283 258 175 124 86 72 52 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 367 1050 Size: 367 683 BsiHKAI G_wGCw'C Cuts at: 0 57 123 392 482 1050 Size: 57 66 269 90 568 Fragments arranged by size: 568 269 90 66 57 Bsp1286I G_dGCh'C Cuts at: 0 45 57 94 123 225 392 482 841 Size: 45 12 37 29 102 167 90 359 Cuts at: 841 1050 Size: 209 Fragments arranged by size: 359 209 167 102 90 45 37 29 12 BspEI T'CCGG_A Cuts at: 0 227 1050 Size: 227 823 BsrDI GCAATG_nn' Cuts at: 0 866 1002 1050 Size: 866 136 48 Fragments arranged by size: 866 136 48 BstAPI GCAn_nnn'nTGC Cuts at: 0 413 1050 Size: 413 637 BstXI CCAn_nnnn'nTGG Cuts at: 0 853 1050 Size: 853 197 BstYI r'GATC_y Cuts at: 0 422 665 1050 Size: 422 243 385 Fragments arranged by size: 422 385 243 BtsI GCAGTG_nn' Cuts at: 0 834 841 1050 Size: 834 7 209 Fragments arranged by size: 834 209 7 EcoICRI GAG'CTC Cuts at: 0 121 1050 Size: 121 929 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 239 290 704 879 1050 Size: 151 88 51 414 175 171 Fragments arranged by size: 414 175 171 151 88 51 EcoNI CCTnn'n_nnAGG Cuts at: 0 686 1050 Size: 686 364 EcoO109I rG'GnC_Cy Cuts at: 0 458 707 962 1050 Size: 458 249 255 88 Fragments arranged by size: 458 255 249 88 HaeII r_GCGC'y Cuts at: 0 214 453 1050 Size: 214 239 597 Fragments arranged by size: 597 239 214 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 162 194 1050 Size: 162 32 856 Fragments arranged by size: 856 162 32 KasI G'GCGC_C Cuts at: 0 449 1050 Size: 449 601 KpnI G_GTAC'C Cuts at: 0 813 1050 Size: 813 237 MspA1I CmG'CkG Cuts at: 0 816 1050 Size: 816 234 NarI GG'CG_CC Cuts at: 0 450 1050 Size: 450 600 NheI G'CTAG_C Cuts at: 0 135 1050 Size: 135 915 PflMI CCAn_nnn'nTGG Cuts at: 0 262 774 1050 Size: 262 512 276 Fragments arranged by size: 512 276 262 PfoI T'CCnGG_A Cuts at: 0 152 767 1050 Size: 152 615 283 Fragments arranged by size: 615 283 152 PmlI CAC'GTG Cuts at: 0 387 1050 Size: 387 663 PpuMI rG'GwC_Cy Cuts at: 0 458 707 1050 Size: 458 249 343 Fragments arranged by size: 458 343 249 PstI C_TGCA'G Cuts at: 0 577 800 829 1048 1050 Size: 577 223 29 219 2 Fragments arranged by size: 577 223 219 29 2 PvuII CAG'CTG Cuts at: 0 816 1050 Size: 816 234 SacI G_AGCT'C Cuts at: 0 123 1050 Size: 123 927 SanDI GG'GwC_CC Cuts at: 0 458 1050 Size: 458 592 SfcI C'TryA_G Cuts at: 0 235 312 573 796 825 1044 1050 Size: 235 77 261 223 29 219 6 Fragments arranged by size: 261 235 223 219 77 29 6 SfoI GGC'GCC Cuts at: 0 451 1050 Size: 451 599 SmlI C'TyrA_G Cuts at: 0 417 531 661 1050 Size: 417 114 130 389 Fragments arranged by size: 417 389 130 114 SnaBI TAC'GTA Cuts at: 0 759 1050 Size: 759 291 StuI AGG'CCT Cuts at: 0 1036 1050 Size: 1036 14 StyI C'CwwG_G Cuts at: 0 439 453 919 1050 Size: 439 14 466 131 Fragments arranged by size: 466 439 131 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 338 1050 Size: 338 712 Tth111I GACn'n_nGTC Cuts at: 0 66 1050 Size: 66 984 Enzymes that do cut: AccI Acc65I AcuI AfeI AflII AhdI AlwNI AvaI BanI BanII BbeI BbsI BciVI BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspEI BsrDI BstAPI BstXI BstYI BtsI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI KpnI MspA1I NarI NheI PflMI PfoI PmlI PpuMI PstI PvuII SacI SanDI SfcI SfoI SmlI SnaBI StuI StyI TaqII Tth111I Enzymes that do not cut: AarI AatII AclI AflIII AgeI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BclI BfrBI BglI BglII BmgBI Bpu10I BsaBI BseYI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI MmeI MscI MslI NaeI NcoI NdeI NgoMIV NotI NruI NsiI NspI PacI PasI PciI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SpeI SphI SrfI SspI SwaI TatI TstI XbaI XcmI XhoI XmaI XmnI ZraI