################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AcyI 1385 GGCGTC G[GA]^CG[CT]C Aor51HI 953 AGCGCT AGC^GCT ApaI 820 GGGCCC GGGCC^C AvaI 287 CCCGAG C^[CT]CG[GA]G BalI 966 TGGCCA TGG^CCA BbrPI 1565 CACGTG CAC^GTG BglII 996 AGATCT A^GATCT BsaAI 1565 CACGTG [CT]AC^GT[GA] BsaHI 1385 GGCGTC G[GA]^CG[CT]C BsaI 1049 GAGACC GGTCTC(1/5) BsgI 1372 GTGCAG GTGCAG(16/14) BsoBI 287 CCCGAG C^[CT]CG[GA]G Bsp1407I 1500 TGTACA T^GTACA BspMI 322 ACCTGC ACCTGC(4/8) BsrDI 347 GCAATG GCAATG(2/0) BsrGI 1500 TGTACA T^GTACA BstAPI 591 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DrdI 763 GACCACATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 316 CTCTTC CTCTTC(1/4) Eco47III 953 AGCGCT AGC^GCT FauI 96 GCGGG CCCGC(4/6) HgaI 1386 GCGTC GACGC(5/10) Hin1I 1385 GGCGTC G[GA]^CG[CT]C Ksp632I 316 CTCTTC CTCTTC(1/4) MaeII 1566 ACGT A^CGT MluNI 966 TGGCCA TGG^CCA MscI 966 TGGCCA TGG^CCA PmaCI 1565 CACGTG CAC^GTG PmlI 1565 CACGTG CAC^GTG PstI 589 CTGCAG CTGCA^G SexAI 978 ACCAGGT A^CC[AT]GGT SmlI 1532 CTCAAG C^T[CT][GA]AG SspBI 1500 TGTACA T^GTACA TfiI 1686 GAATC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwI 188 GGATC GGATC(4/5) 1033 GGATC BanI 1144 GGCACC G^G[CT][GA]CC 1161 GGCACC BbsI 1453 GAAGAC GAAGAC(2/6) 1591 GAAGAC BglI 682 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1315 GCCTATGAGGC BpuAI 1453 GAAGAC GAAGAC(2/6) 1591 GAAGAC BsmAI 239 GAGAC GTCTC(1/5) 1049 GAGAC BstYI 188 GGATCT [GA]^GATC[CT] 996 AGATCT EaeI 804 TGGCCG [CT]^GGCC[GA] 966 TGGCCA EcoNI 370 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 921 CCTGGCCCAGG MaeIII 275 GTGAC ^GT[ACGT]AC 887 GTGAC MflI 188 GGATCT [GA]^GATC[CT] 996 AGATCT MslI 36 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 432 CATCTGGATG PflMI 884 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 927 CCAGGGCCTGG PpuMI 405 GGGTCCC [GA]G^G[AT]CC[CT] 798 GGGTCCT PshAI 277 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1288 GACAGCAGTC Tsp45I 275 GTGAC ^GT[GC]AC 887 GTGAC Tsp509I 355 AATT ^AATT 1605 AATT Van91I 884 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 927 CCAGGGCCTGG XcmI 294 CCACCCCAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1043 CCATTGGAGACCTGG XhoII 188 GGATCT [GA]^GATC[CT] 996 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AfaI AluI AlwNI AspHI AvaII BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TseI TspRI