(Circular) (Six-base) MAPSORT of: pFN21AB5989.seq Check: 6015 from: 1 to: 6424 pFN21AB5989 6424 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2336 2336 Size: 6424 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4234 278 Size: 53 83 186 936 2698 2468 Fragments arranged by size: 2698 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3767 1937 Size: 1830 4594 Acc65I G'GTAC_C Cuts at: 3745 3745 Size: 6424 AclI AA'CG_TT Cuts at: 1563 4551 4924 1563 Size: 2988 373 3063 Fragments arranged by size: 3063 2988 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3646 4487 5871 6139 1632 Size: 2014 841 1384 268 1917 Fragments arranged by size: 2014 1917 1384 841 268 AfeI AGC'GCT Cuts at: 2960 2960 Size: 6424 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6227 AflIII A'CryG_T Cuts at: 1203 5323 6300 1203 Size: 4120 977 1327 Fragments arranged by size: 4120 1327 977 AgeI A'CCGG_T Cuts at: 5255 5255 Size: 6424 AhdI GACnn_n'nnGTC Cuts at: 5153 5153 Size: 6424 AleI CACnn'nnGTG Cuts at: 1166 2045 1166 Size: 879 5545 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6424 AlwNI CAG_nnn'CTG Cuts at: 2181 2580 2602 2895 2938 3434 3517 4167 5739 Size: 399 22 293 43 496 83 650 1572 Cuts at: 5739 2181 Size: 2866 Fragments arranged by size: 2866 1572 650 496 399 293 83 43 22 ApaI G_GGCC'C Cuts at: 2829 2829 Size: 6424 ApaLI G'TGCA_C Cuts at: 4481 5637 4481 Size: 1156 5268 ApoI r'AATT_y Cuts at: 1493 3733 3959 4111 6220 1493 Size: 2240 226 152 2109 1697 Fragments arranged by size: 2240 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4978 160 Size: 872 3946 1606 Fragments arranged by size: 3946 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6424 AvaI C'yCGr_G Cuts at: 1870 1943 2292 3749 1870 Size: 73 349 1457 4545 Fragments arranged by size: 4545 1457 349 73 BamHI G'GATC_C Cuts at: 3754 4121 3754 Size: 367 6057 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3149 3166 3745 5200 618 Size: 325 217 627 1362 17 579 1455 1842 Fragments arranged by size: 1842 1455 1362 627 579 325 217 17 BanII G_rGCy'C Cuts at: 1391 2151 2299 2390 2829 2861 3177 3453 3743 Size: 760 148 91 439 32 316 276 290 Cuts at: 3743 1391 Size: 4072 Fragments arranged by size: 4072 760 439 316 290 276 148 91 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3465 3603 928 Size: 971 1566 138 3749 Fragments arranged by size: 3749 1566 971 138 BbvCI CC'TCA_GC Cuts at: 3443 3642 3696 3443 Size: 199 54 6171 Fragments arranged by size: 6171 199 54 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4615 4649 1903 Size: 34 50 34 2594 34 3678 Fragments arranged by size: 3678 2594 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4316 5526 4316 Size: 1210 5214 BclI T'GATC_A Cuts at: 1548 1548 Size: 6424 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2693 3326 5035 Size: 107 122 71 631 605 1021 633 1709 Cuts at: 5035 136 Size: 1525 Fragments arranged by size: 1709 1525 1021 633 631 605 122 107 71 BglII A'GATC_T Cuts at: 3001 6419 3001 Size: 3418 3006 BlpI GC'TnA_GC Cuts at: 3841 3841 Size: 6424 Bme1580I G_kGCm'C Cuts at: 1790 2221 2829 4485 5641 1790 Size: 431 608 1656 1156 2573 Fragments arranged by size: 2573 1656 1156 608 431 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6424 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2129 2875 2905 Size: 416 53 83 399 24 712 746 30 Cuts at: 2905 3344 4155 5113 442 Size: 439 811 958 1753 Fragments arranged by size: 1753 958 811 746 712 439 416 399 83 53 30 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6424 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2115 2364 2602 5084 1239 Size: 876 249 238 2482 2579 Fragments arranged by size: 2579 2482 876 249 238 Bpu10I CC'TnA_GC Cuts at: 2607 3443 3642 3696 2607 Size: 836 199 54 5335 Fragments arranged by size: 5335 836 199 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3522 4549 5414 5712 3522 Size: 1027 865 298 4234 Fragments arranged by size: 4234 1027 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3048 5087 882 Size: 617 1549 2039 2219 Fragments arranged by size: 2219 2039 1549 617 BsaAI yAC'GTr Cuts at: 493 1204 3572 4215 493 Size: 711 2368 643 2702 Fragments arranged by size: 2702 2368 711 643 BsaBI GATnn'nnATC Cuts at: 6418 6418 Size: 6424 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3391 4231 4613 5248 Size: 53 83 186 936 1858 840 382 635 Cuts at: 5248 275 Size: 1451 Fragments arranged by size: 1858 1451 936 840 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4857 5255 5529 5676 6087 4857 Size: 398 274 147 411 5194 Fragments arranged by size: 5194 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3700 3730 1178 Size: 30 165 30 2297 30 3872 Fragments arranged by size: 3872 2297 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2367 2462 2465 2969 3400 1186 Size: 1181 95 3 504 431 4210 Fragments arranged by size: 4210 1181 504 431 95 3 BseYI C'CCAG_C Cuts at: 2303 2458 2488 3127 5627 6123 2303 Size: 155 30 639 2500 496 2604 Fragments arranged by size: 2604 2500 639 496 155 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3398 1163 Size: 573 1662 4189 Fragments arranged by size: 4189 1662 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3874 4635 5663 1540 Size: 275 186 1873 761 1028 2301 Fragments arranged by size: 2301 1873 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2151 3024 3078 3177 3453 4485 4570 5641 2151 Size: 873 54 99 276 1032 85 1071 2934 Fragments arranged by size: 2934 1071 1032 873 276 99 85 54 BsmI GAATG_Cn' Cuts at: 3935 4028 3935 Size: 93 6331 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2151 2221 2299 2390 2829 2861 3024 Size: 399 361 70 78 91 439 32 163 Cuts at: 3024 3078 3177 3453 3743 4485 4570 5641 1391 Size: 54 99 276 290 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 439 399 361 290 276 163 99 91 85 78 70 54 32 BspEI T'CCGG_A Cuts at: 6087 6087 Size: 6424 BspHI T'CATG_A Cuts at: 4313 6043 4313 Size: 1730 4694 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2336 3779 844 Size: 1492 1443 3489 Fragments arranged by size: 3489 1492 1443 BsrBI CCG'CTC Cuts at: 1394 1823 4311 1394 Size: 429 2488 3507 Fragments arranged by size: 3507 2488 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2359 4919 5093 65 Size: 1183 1111 2560 174 1396 Fragments arranged by size: 2560 1396 1183 1111 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5068 5255 1700 Size: 110 3258 187 2869 Fragments arranged by size: 3258 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3505 96 Size: 3409 3015 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6424 BssSI C'ACGA_G Cuts at: 4484 5496 4484 Size: 1012 5412 BstAPI GCAn_nnn'nTGC Cuts at: 794 2602 794 Size: 1808 4616 BstBI TT'CG_AA Cuts at: 5228 5228 Size: 6424 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6424 BstXI CCAn_nnnn'nTGG Cuts at: 2047 2443 3411 2047 Size: 396 968 5060 Fragments arranged by size: 5060 968 396 BstYI r'GATC_y Cuts at: 1972 2193 3001 3754 4121 4506 4523 5975 6061 Size: 221 808 753 367 385 17 1452 86 Cuts at: 6061 6073 6419 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 808 753 385 367 346 221 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5872 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5685 Fragments arranged by size: 5685 683 56 BtsI GCAGTG_nn' Cuts at: 2655 2787 3381 3940 4734 4754 2655 Size: 132 594 559 794 20 4325 Fragments arranged by size: 4325 794 594 559 132 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6424 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6389 DraI TTT'AAA Cuts at: 3728 3728 Size: 6424 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2774 5431 809 Size: 507 1458 2657 1802 Fragments arranged by size: 2657 1802 1458 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2809 2971 3871 Size: 54 1425 50 275 18 979 162 900 Cuts at: 3871 4760 6389 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 979 900 889 275 162 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3871 1537 Size: 275 2059 4090 Fragments arranged by size: 4090 2059 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2327 4354 1353 Size: 974 2027 3423 Fragments arranged by size: 3423 2027 974 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5011 5385 5531 387 Size: 1064 138 3422 374 146 1280 Fragments arranged by size: 3422 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2149 3175 3451 2149 Size: 1026 276 5122 Fragments arranged by size: 5122 1026 276 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2364 2602 3646 4487 5084 5871 Size: 393 483 249 238 1044 841 597 787 Cuts at: 5871 6139 1239 Size: 268 1524 Fragments arranged by size: 1524 1044 841 787 597 483 393 268 249 238 EcoNI CCTnn'n_nnAGG Cuts at: 2379 2930 2379 Size: 551 5873 EcoO109I rG'GnC_Cy Cuts at: 2089 2411 2804 2826 2935 3677 2089 Size: 322 393 22 109 742 4836 Fragments arranged by size: 4836 742 393 322 109 22 EcoRI G'AATT_C Cuts at: 3733 4111 3733 Size: 378 6046 FspI TGC'GCA Cuts at: 4930 4930 Size: 6424 HaeII r_GCGC'y Cuts at: 1678 2962 3418 3517 5571 1678 Size: 1284 456 99 2054 2531 Fragments arranged by size: 2531 2054 1284 456 99 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3097 3129 3221 3253 3376 Size: 32 135 32 1525 32 92 32 123 Cuts at: 3376 3408 5065 5097 5139 5171 1373 Size: 32 1657 32 42 32 2626 Fragments arranged by size: 2626 1657 1525 135 123 92 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3768 4014 669 Size: 988 281 1830 246 3079 Fragments arranged by size: 3079 1830 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6424 HpaI GTT'AAC Cuts at: 4014 4014 Size: 6424 KpnI G_GTAC'C Cuts at: 3749 3749 Size: 6424 MfeI C'AATT_G Cuts at: 4023 4023 Size: 6424 MluI A'CGCG_T Cuts at: 6300 6300 Size: 6424 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3244 3337 5538 5722 1305 Size: 1939 93 2201 184 2007 Fragments arranged by size: 2201 2007 1939 184 93 MscI TGG'CCA Cuts at: 10 64 1489 1832 2973 10 Size: 54 1425 343 1141 3461 Fragments arranged by size: 3461 1425 1141 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2045 2441 4382 4741 4900 518 Size: 648 879 396 1941 359 159 2042 Fragments arranged by size: 2042 1941 879 648 396 359 159 MspA1I CmG'CkG Cuts at: 1606 2045 2166 2181 2668 3131 3273 3497 3646 Size: 439 121 15 487 463 142 224 149 Cuts at: 3646 3840 4517 5665 5910 1606 Size: 194 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 487 463 439 245 224 194 149 142 121 15 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5872 NdeI CA'TA_TG Cuts at: 387 387 Size: 6424 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6424 NotI GC'GGCC_GC Cuts at: 3871 3871 Size: 6424 NspI r_CATG'y Cuts at: 1132 1229 3782 5327 1132 Size: 97 2553 1545 2229 Fragments arranged by size: 2553 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2390 2407 2932 1348 Size: 1042 17 525 4840 Fragments arranged by size: 4840 1042 525 17 PciI A'CATG_T Cuts at: 5323 5323 Size: 6424 PflMI CCAn_nnn'nTGG Cuts at: 1108 2895 2938 1108 Size: 1787 43 4594 Fragments arranged by size: 4594 1787 43 PfoI T'CCnGG_A Cuts at: 2631 3686 2631 Size: 1055 5369 PmeI GTTT'AAAC Cuts at: 3728 3728 Size: 6424 PmlI CAC'GTG Cuts at: 3572 3572 Size: 6424 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4493 4525 6006 6038 4493 Size: 32 1481 32 4879 Fragments arranged by size: 4879 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2411 2804 2411 Size: 393 6031 PshAI GACnn'nnGTC Cuts at: 1613 2286 3297 1613 Size: 673 1011 4740 Fragments arranged by size: 4740 1011 673 PsiI TTA'TAA Cuts at: 3994 3994 Size: 6424 PspOMI G'GGCC_C Cuts at: 2825 2825 Size: 6424 PstI C_TGCA'G Cuts at: 830 2598 3776 830 Size: 1768 1178 3478 Fragments arranged by size: 3478 1768 1178 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6424 PvuII CAG'CTG Cuts at: 2045 2668 3131 3497 2045 Size: 623 463 366 4972 Fragments arranged by size: 4972 623 463 366 SacI G_AGCT'C Cuts at: 2151 3177 3453 2151 Size: 1026 276 5122 Fragments arranged by size: 5122 1026 276 SalI G'TCGA_C Cuts at: 1936 3766 1936 Size: 1830 4594 SanDI GG'GwC_CC Cuts at: 2411 2411 Size: 6424 SbfI CC_TGCA'GG Cuts at: 3776 3776 Size: 6424 ScaI AGT'ACT Cuts at: 4672 6384 4672 Size: 1712 4712 SexAI A'CCwGG_T Cuts at: 2983 2983 Size: 6424 SfcI C'TryA_G Cuts at: 826 1045 2173 2513 2527 2594 2759 3197 3634 Size: 219 1128 340 14 67 165 438 437 Cuts at: 3634 3772 4907 5588 5779 826 Size: 138 1135 681 191 1471 Fragments arranged by size: 1471 1135 1128 681 438 437 340 219 191 165 138 67 14 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6424 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6424 SmaI CCC'GGG Cuts at: 1872 3751 1872 Size: 1879 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3537 4528 5429 5691 820 Size: 197 926 1594 991 901 262 1553 Fragments arranged by size: 1594 1553 991 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4215 493 Size: 3722 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6424 SphI G_CATG'C Cuts at: 1132 3782 1132 Size: 2650 3774 SspI AAT'ATT Cuts at: 5 52 4348 5 Size: 47 4296 2081 Fragments arranged by size: 4296 2081 47 StuI AGG'CCT Cuts at: 1517 2696 2757 3289 3640 1517 Size: 1179 61 532 351 4301 Fragments arranged by size: 4301 1179 532 351 61 StyI C'CwwG_G Cuts at: 513 1065 2558 2853 3069 3369 3525 3863 513 Size: 552 1493 295 216 300 156 338 3074 Fragments arranged by size: 3074 1493 552 338 300 295 216 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3167 4451 4468 4621 4806 5251 325 Size: 1255 1587 1284 17 153 185 445 1498 Fragments arranged by size: 1587 1498 1284 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2883 3505 3602 Size: 274 80 33 51 1443 906 622 97 Cuts at: 3602 4670 6382 96 Size: 1068 1712 138 Fragments arranged by size: 1712 1443 1068 906 622 274 138 97 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3294 3326 6384 6416 3294 Size: 32 3058 32 3302 Fragments arranged by size: 3302 3058 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6424 XbaI T'CTAG_A Cuts at: 3760 3760 Size: 6424 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2306 3055 2306 Size: 749 5675 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6424 XmaI C'CCGG_G Cuts at: 1870 3749 1870 Size: 1879 4545 XmnI GAAnn'nnTTC Cuts at: 4553 4553 Size: 6424 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4232 276 Size: 53 83 186 936 2698 2468 Fragments arranged by size: 2698 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI