(Circular) (Six-base) MAPSORT of: pFN21AB8524.seq Check: 8459 from: 1 to: 7216 pFN21AB8524 7216 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2170 2170 Size: 7216 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5026 278 Size: 53 83 186 936 3490 2468 Fragments arranged by size: 3490 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2265 4559 1937 Size: 328 2294 4594 Fragments arranged by size: 4594 2294 328 Acc65I G'GTAC_C Cuts at: 3319 4537 3319 Size: 1218 5998 AclI AA'CG_TT Cuts at: 1563 5343 5716 1563 Size: 3780 373 3063 Fragments arranged by size: 3780 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2138 3180 3362 3510 5279 6663 6931 1632 Size: 506 1042 182 148 1769 1384 268 1917 Fragments arranged by size: 1917 1769 1384 1042 506 268 182 148 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7019 AflIII A'CryG_T Cuts at: 1203 6115 7092 1203 Size: 4912 977 1327 Fragments arranged by size: 4912 1327 977 AgeI A'CCGG_T Cuts at: 6047 6047 Size: 7216 AhdI GACnn_n'nnGTC Cuts at: 5945 5945 Size: 7216 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7216 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7216 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2843 2875 2843 Size: 32 7184 AlwNI CAG_nnn'CTG Cuts at: 2068 3340 4253 4959 6531 2068 Size: 1272 913 706 1572 2753 Fragments arranged by size: 2753 1572 1272 913 706 ApaI G_GGCC'C Cuts at: 2922 2922 Size: 7216 ApaLI G'TGCA_C Cuts at: 5273 6429 5273 Size: 1156 6060 ApoI r'AATT_y Cuts at: 1493 3394 4525 4751 4903 7012 1493 Size: 1901 1131 226 152 2109 1697 Fragments arranged by size: 2109 1901 1697 1131 226 152 AseI AT'TA_AT Cuts at: 160 1032 5770 160 Size: 872 4738 1606 Fragments arranged by size: 4738 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7216 AvaI C'yCGr_G Cuts at: 1870 1943 3083 4219 4541 1870 Size: 73 1140 1136 322 4545 Fragments arranged by size: 4545 1140 1136 322 73 BamHI G'GATC_C Cuts at: 2169 4546 4913 2169 Size: 2377 367 4472 Fragments arranged by size: 4472 2377 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2018 2621 2827 3319 4537 Size: 325 217 627 231 603 206 492 1218 Cuts at: 4537 5992 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1218 627 603 492 325 231 217 206 BanII G_rGCy'C Cuts at: 1391 2482 2802 2885 2922 3738 3988 4535 1391 Size: 1091 320 83 37 816 250 547 4072 Fragments arranged by size: 4072 1091 816 547 320 250 83 37 BbeI G_GCGC'C Cuts at: 2022 2022 Size: 7216 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3237 3285 4469 928 Size: 971 1338 48 1184 3675 Fragments arranged by size: 3675 1338 1184 971 48 BbvCI CC'TCA_GC Cuts at: 2906 4130 2906 Size: 1224 5992 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4346 4380 5407 5441 1903 Size: 34 2409 34 1027 34 3678 Fragments arranged by size: 3678 2409 1027 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5108 6318 5108 Size: 1210 6006 BclI T'GATC_A Cuts at: 1548 1548 Size: 7216 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2145 2619 2717 Size: 107 122 71 631 605 473 474 98 Cuts at: 2717 5827 136 Size: 3110 1525 Fragments arranged by size: 3110 1525 631 605 474 473 122 107 98 71 BglII A'GATC_T Cuts at: 7211 7211 Size: 7216 BlpI GC'TnA_GC Cuts at: 4633 4633 Size: 7216 Bme1580I G_kGCm'C Cuts at: 1790 2532 2832 2922 4420 5277 6433 1790 Size: 742 300 90 1498 857 1156 2573 Fragments arranged by size: 2573 1498 1156 857 742 300 90 BmgBI CAC'GTC Cuts at: 1318 3856 1318 Size: 2538 4678 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2916 4947 5905 Size: 416 53 83 399 24 1499 2031 958 Cuts at: 5905 442 Size: 1753 Fragments arranged by size: 2031 1753 1499 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7216 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2451 2604 2451 Size: 153 7063 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2071 2468 3083 3317 3746 5876 1239 Size: 832 397 615 234 429 2130 2579 Fragments arranged by size: 2579 2130 832 615 429 397 234 Bpu10I CC'TnA_GC Cuts at: 2906 4130 2906 Size: 1224 5992 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2446 3182 3280 3401 5341 6206 6504 2446 Size: 736 98 121 1940 865 298 3158 Fragments arranged by size: 3158 1940 865 736 298 121 98 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5879 882 Size: 617 4380 2219 Fragments arranged by size: 4380 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2730 5007 493 Size: 711 1526 2277 2702 Fragments arranged by size: 2702 2277 1526 711 BsaBI GATnn'nnATC Cuts at: 7210 7210 Size: 7216 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2019 5023 5405 6040 Size: 53 83 186 936 486 3004 382 635 Cuts at: 6040 275 Size: 1451 Fragments arranged by size: 3004 1451 936 635 486 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2043 3774 5649 6047 6321 6468 6879 2043 Size: 1731 1875 398 274 147 411 2380 Fragments arranged by size: 2380 1875 1731 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3887 3917 1178 Size: 30 165 30 2484 30 4477 Fragments arranged by size: 4477 2484 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2455 2962 1186 Size: 1269 507 5440 Fragments arranged by size: 5440 1269 507 BseYI C'CCAG_C Cuts at: 2023 2370 2525 2915 3030 3034 6419 6915 2023 Size: 347 155 390 115 4 3385 496 2324 Fragments arranged by size: 3385 2324 496 390 347 155 115 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2204 2522 4371 1163 Size: 573 468 318 1849 4008 Fragments arranged by size: 4008 1849 573 468 318 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2140 2979 4005 4666 5427 6455 Size: 275 186 139 839 1026 661 761 1028 Cuts at: 6455 1540 Size: 2301 Fragments arranged by size: 2301 1028 1026 839 761 661 275 186 139 BsiHKAI G_wGCw'C Cuts at: 2885 3738 3988 5277 5362 6433 2885 Size: 853 250 1289 85 1071 3668 Fragments arranged by size: 3668 1289 1071 853 250 85 BsmI GAATG_Cn' Cuts at: 3046 3699 3907 4303 4727 4820 3046 Size: 653 208 396 424 93 5442 Fragments arranged by size: 5442 653 424 396 208 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2482 2532 2802 2832 2885 2922 3738 Size: 399 692 50 270 30 53 37 816 Cuts at: 3738 3988 4420 4535 5277 5362 6433 1391 Size: 250 432 115 742 85 1071 2174 Fragments arranged by size: 2174 1071 816 742 692 432 399 270 250 115 85 53 50 37 30 BspEI T'CCGG_A Cuts at: 2043 6879 2043 Size: 4836 2380 BspHI T'CATG_A Cuts at: 3426 5105 6835 3426 Size: 1679 1730 3807 Fragments arranged by size: 3807 1730 1679 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2170 2803 4571 844 Size: 1326 633 1768 3489 Fragments arranged by size: 3489 1768 1326 633 BsrBI CCG'CTC Cuts at: 1394 1823 5103 1394 Size: 429 3280 3507 Fragments arranged by size: 3507 3280 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3440 3651 5711 5885 65 Size: 1183 2192 211 2060 174 1396 Fragments arranged by size: 2192 2060 1396 1183 211 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5860 6047 1700 Size: 110 4050 187 2869 Fragments arranged by size: 4050 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7216 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7216 BssSI C'ACGA_G Cuts at: 3140 5276 6288 3140 Size: 2136 1012 4068 Fragments arranged by size: 4068 2136 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2536 4358 794 Size: 1742 1822 3652 Fragments arranged by size: 3652 1822 1742 BstBI TT'CG_AA Cuts at: 6020 6020 Size: 7216 BstEII G'GTnAC_C Cuts at: 1184 4094 1184 Size: 2910 4306 BstXI CCAn_nnnn'nTGG Cuts at: 3188 3358 3892 3188 Size: 170 534 6512 Fragments arranged by size: 6512 534 170 BstYI r'GATC_y Cuts at: 1972 2064 2169 4546 4913 5298 5315 6767 6853 Size: 92 105 2377 367 385 17 1452 86 Cuts at: 6853 6865 7211 1972 Size: 12 346 1977 Fragments arranged by size: 2377 1977 1452 385 367 346 105 92 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3979 3979 Size: 7216 BtgI C'CryG_G Cuts at: 513 1065 2004 2140 2217 2409 2931 3681 513 Size: 552 939 136 77 192 522 750 4048 Fragments arranged by size: 4048 939 750 552 522 192 136 77 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3611 4371 487 Size: 683 56 2385 760 3332 Fragments arranged by size: 3332 2385 760 683 56 BtsI GCAGTG_nn' Cuts at: 2254 3078 4732 5526 5546 2254 Size: 824 1654 794 20 3924 Fragments arranged by size: 3924 1654 824 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7216 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7181 DraI TTT'AAA Cuts at: 4520 4520 Size: 7216 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3629 6223 809 Size: 507 2313 2594 1802 Fragments arranged by size: 2594 2313 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2137 2146 2968 Size: 54 1425 50 275 18 307 9 822 Cuts at: 2968 2976 4002 4341 4663 5552 7181 8 Size: 8 1026 339 322 889 1629 43 Fragments arranged by size: 1629 1425 1026 889 822 339 322 307 275 54 50 43 18 9 8 EagI C'GGCC_G Cuts at: 1537 1812 2137 2976 4002 4663 1537 Size: 275 325 839 1026 661 4090 Fragments arranged by size: 4090 1026 839 661 325 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2371 3164 5146 1353 Size: 1018 793 1982 3423 Fragments arranged by size: 3423 1982 1018 793 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5803 6177 6323 387 Size: 1064 138 4214 374 146 1280 Fragments arranged by size: 4214 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2883 3736 3986 2883 Size: 853 250 6113 Fragments arranged by size: 6113 853 250 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2071 2138 2468 3083 3180 3317 3362 Size: 393 439 67 330 615 97 137 45 Cuts at: 3362 3510 3746 5279 5876 6663 6931 1239 Size: 148 236 1533 597 787 268 1524 Fragments arranged by size: 1533 1524 787 615 597 439 393 330 268 236 148 137 97 67 45 EcoO109I rG'GnC_Cy Cuts at: 2421 2865 3255 2421 Size: 444 390 6382 Fragments arranged by size: 6382 444 390 EcoRI G'AATT_C Cuts at: 4525 4903 4525 Size: 378 6838 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3461 3461 Size: 7216 FspI TGC'GCA Cuts at: 5722 5722 Size: 7216 HaeII r_GCGC'y Cuts at: 1678 2022 2099 2641 3582 6363 1678 Size: 344 77 542 941 2781 2531 Fragments arranged by size: 2781 2531 941 542 344 77 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5857 5889 5931 5963 1373 Size: 32 135 32 4285 32 42 32 2626 Fragments arranged by size: 4285 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3178 4477 4560 4806 669 Size: 988 281 1240 1299 83 246 3079 Fragments arranged by size: 3079 1299 1240 988 281 246 83 HindIII A'AGCT_T Cuts at: 748 3200 748 Size: 2452 4764 HpaI GTT'AAC Cuts at: 3178 4806 3178 Size: 1628 5588 KasI G'GCGC_C Cuts at: 2018 2018 Size: 7216 KpnI G_GTAC'C Cuts at: 3323 4541 3323 Size: 1218 5998 MfeI C'AATT_G Cuts at: 4815 4815 Size: 7216 MluI A'CGCG_T Cuts at: 7092 7092 Size: 7216 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2278 4395 6330 6514 1305 Size: 973 2117 1935 184 2007 Fragments arranged by size: 2117 2007 1935 973 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4343 10 Size: 54 1425 343 2511 2883 Fragments arranged by size: 2883 2511 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3053 4092 5174 5533 5692 518 Size: 648 1887 1039 1082 359 159 2042 Fragments arranged by size: 2042 1887 1082 1039 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2027 2136 2142 2824 2943 3034 3724 4632 Size: 421 109 6 682 119 91 690 908 Cuts at: 4632 5309 6457 6702 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 908 690 682 677 421 245 119 109 91 6 NarI GG'CG_CC Cuts at: 2019 2019 Size: 7216 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5725 Fragments arranged by size: 5725 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7216 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7216 NotI GC'GGCC_GC Cuts at: 2137 2976 4663 2137 Size: 839 1687 4690 Fragments arranged by size: 4690 1687 839 NspI r_CATG'y Cuts at: 1132 1229 2335 3100 4091 4574 6119 1132 Size: 97 1106 765 991 483 1545 2229 Fragments arranged by size: 2229 1545 1106 991 765 483 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7216 PciI A'CATG_T Cuts at: 6115 6115 Size: 7216 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7216 PfoI T'CCnGG_A Cuts at: 2172 3107 4142 2172 Size: 935 1035 5246 Fragments arranged by size: 5246 1035 935 PmeI GTTT'AAAC Cuts at: 4520 4520 Size: 7216 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5285 5317 6798 6830 5285 Size: 32 1481 32 5671 Fragments arranged by size: 5671 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2421 2865 2421 Size: 444 6772 PshAI GACnn'nnGTC Cuts at: 1613 4043 1613 Size: 2430 4786 PsiI TTA'TAA Cuts at: 3920 4786 3920 Size: 866 6350 PspOMI G'GGCC_C Cuts at: 2918 2918 Size: 7216 PspXI vC'TCGA_Gb Cuts at: 3083 3083 Size: 7216 PstI C_TGCA'G Cuts at: 830 2838 3124 3210 3993 4068 4161 4568 830 Size: 2008 286 86 783 75 93 407 3478 Fragments arranged by size: 3478 2008 783 407 286 93 86 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7216 PvuII CAG'CTG Cuts at: 2027 3034 3724 2027 Size: 1007 690 5519 Fragments arranged by size: 5519 1007 690 RsrII CG'GwC_CG Cuts at: 2046 2046 Size: 7216 SacI G_AGCT'C Cuts at: 2885 3738 3988 2885 Size: 853 250 6113 Fragments arranged by size: 6113 853 250 SacII CC_GC'GG Cuts at: 2143 2143 Size: 7216 SalI G'TCGA_C Cuts at: 1936 4558 1936 Size: 2622 4594 SbfI CC_TGCA'GG Cuts at: 4568 4568 Size: 7216 ScaI AGT'ACT Cuts at: 4225 5464 7176 4225 Size: 1239 1712 4265 Fragments arranged by size: 4265 1712 1239 SfcI C'TryA_G Cuts at: 826 1045 2834 3120 3206 3989 4064 4157 4245 Size: 219 1789 286 86 783 75 93 88 Cuts at: 4245 4564 5699 6380 6571 826 Size: 319 1135 681 191 1471 Fragments arranged by size: 1789 1471 1135 783 681 319 286 219 191 93 88 86 75 SfiI GGCCn_nnn'nGGCC Cuts at: 2145 2145 Size: 7216 SfoI GGC'GCC Cuts at: 2020 2020 Size: 7216 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7216 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7216 SmaI CCC'GGG Cuts at: 1872 4543 1872 Size: 2671 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2425 3083 3197 3259 3416 5320 Size: 197 926 482 658 114 62 157 1904 Cuts at: 5320 6221 6483 820 Size: 901 262 1553 Fragments arranged by size: 1904 1553 926 901 658 482 262 197 157 114 62 SnaBI TAC'GTA Cuts at: 493 5007 493 Size: 4514 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7216 SphI G_CATG'C Cuts at: 1132 4574 1132 Size: 3442 3774 SspI AAT'ATT Cuts at: 5 52 3749 5140 5 Size: 47 3697 1391 2081 Fragments arranged by size: 3697 2081 1391 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7216 StyI C'CwwG_G Cuts at: 513 1065 2004 3885 4655 513 Size: 552 939 1881 770 3074 Fragments arranged by size: 3074 1881 939 770 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3471 5243 5260 5413 5598 6043 325 Size: 1255 1891 1772 17 153 185 445 1498 Fragments arranged by size: 1891 1772 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2391 2505 4223 Size: 274 80 33 51 1443 414 114 1718 Cuts at: 4223 5462 7174 96 Size: 1239 1712 138 Fragments arranged by size: 1718 1712 1443 1239 414 274 138 114 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3887 3919 7176 7208 3887 Size: 32 3257 32 3895 Fragments arranged by size: 3895 3257 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4214 1316 Size: 2898 4318 XbaI T'CTAG_A Cuts at: 4552 4552 Size: 7216 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2119 2755 2929 4055 2119 Size: 636 174 1126 5280 Fragments arranged by size: 5280 1126 636 174 XhoI C'TCGA_G Cuts at: 1943 3083 1943 Size: 1140 6076 XmaI C'CCGG_G Cuts at: 1870 4541 1870 Size: 2671 4545 XmnI GAAnn'nnTTC Cuts at: 3234 5345 3234 Size: 2111 5105 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5024 276 Size: 53 83 186 936 3490 2468 Fragments arranged by size: 3490 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PsrI SanDI SapI SexAI SrfI SwaI