################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccI AccII AciI AclI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BanI BbeI BbrPI BcgI BcnI BfaI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaI BsgI BsiEI BsiHKAI BsiWI BsoBI Bsp1407I BspDI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MfeI MluI MluNI MscI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccIII 655 TCCGGA T^CCGGA AcyI 661 GACGCC G[GA]^CG[CT]C AflIII 899 ACATGT A^C[GA][CT]GT AocI 920 CCTCAGG CC^T[ACGT]AGG AspI 100 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 755 GGACC G^G[AT]CC BamHI 137 GGATCC G^GATCC BanII 916 GAGCCC G[GA]GC[CT]^C BbsI 415 GTCTTC GAAGAC(2/6) BbvCI 891 GCTGAGG CCTCAGC(-5/-2) BciVI 472 GGATAC GTATCC(6/5) BglII 119 AGATCT A^GATCT BmyI 916 GAGCCC G[AGT]GC[ACT]^C BpuAI 415 GTCTTC GAAGAC(2/6) BsaBI 441 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 661 GACGCC G[GA]^CG[CT]C BsaWI 655 TCCGGA [AT]^CCGG[AT] BseAI 655 TCCGGA T^CCGGA BsmBI 659 GAGACG CGTCTC(1/5) BsmFI 579 GTCCC GGGAC(10/14) BsmI 248 GAATGC GAATGC(1/-1) Bsp1286I 916 GAGCCC G[AGT]GC[ACT]^C BspEI 655 TCCGGA T^CCGGA BspLU11I 899 ACATGT A^CATGT BsrDI 586 GCAATG GCAATG(2/0) BstF5I 447 CATCC GGATG(2/0) Bsu36I 920 CCTCAGG CC^T[ACGT]AGG Cac8I 664 GCCAGC GC[ACGT]^[ACGT]GC Cfr13I 755 GGACC G^G[ACGT]CC DraI 426 TTTAAA TTT^AAA EarI 123 CTCTTC CTCTTC(1/4) Eco81I 920 CCTCAGG CC^T[ACGT]AGG EcoNI 758 CCTCTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FokI 447 CATCC GGATG(9/13) HapII 656 CCGG C^CGG HgaI 661 GACGC GACGC(5/10) Hin1I 661 GACGCC G[GA]^CG[CT]C HpaII 656 CCGG C^CGG Ksp632I 123 CTCTTC CTCTTC(1/4) MamI 441 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 655 TCCGGA T^CCGGA MspI 656 CCGG C^CGG NspI 899 ACATGT [GA]CATG^[CT] PflFI 100 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 795 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 408 GACTTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Sau96I 755 GGACC G^G[ACGT]CC SfaNI 633 GATGC GCATC(5/9) SfcI 299 CTACAG C^T[GA][CT]AG SmlI 325 CTCAAG C^T[CT][GA]AG TaqI 162 TCGA T^CGA Tth111I 100 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 162 TCGA T^CGA Van91I 795 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AfaI 311 GTAC GT^AC 897 GTAC AlwI 138 GATCC GGATC(4/5) 137 GGATC BbvI 636 GCAGC GCAGC(8/12) 396 GCTGC BclI 272 TGATCA T^GATCA 698 TGATCA BseRI 598 CTCCTC GAGGAG(10/8) 683 GAGGAG BsiYI 758 CCTCTGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 795 CCAGGTGATGG BslI 758 CCTCTGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 795 CCAGGTGATGG BsmAI 659 GAGAC GTCTC(1/5) 861 GAGAC BstYI 119 AGATCT [GA]^GATC[CT] 137 GGATCC FbaI 272 TGATCA T^GATCA 698 TGATCA Fnu4HI 396 GCTGC GC^[ACGT]GC 636 GCAGC HaeIII 304 GGCC GG^CC 867 GGCC HphI 87 GGTGA GGTGA(8/7) 798 GGTGA ItaI 396 GCTGC GC^[ACGT]GC 636 GCAGC MflI 119 AGATCT [GA]^GATC[CT] 137 GGATCC MwoI 220 GCTTTATTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 882 GCTACTGTGGC NlaIV 137 GGATCC GG[ACGT]^[ACGT]CC 915 GGAGCC RsaI 311 GTAC GT^AC 897 GTAC SspI 183 AATATT AAT^ATT 430 AATATT StuI 303 AGGCCT AGG^CCT 866 AGGCCT TseI 396 GCTGC G^C[AT]GC 636 GCAGC XhoII 119 AGATCT [GA]^GATC[CT] 137 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AcsI AluI ApoI BsaJI BsrI BssKI BstNI DdeI DpnII EcoRII HinfI MaeIII MboI MboII MnlI MseI MslI MvaI NdeII NlaIII Sau3AI ScrFI TfiI Tru9I Tsp45I Tsp509I TspRI