################################## # NO CUT ENZYMES ( 183 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV FauI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AfaI 82 GTAC GT^AC AspI 100 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvI 886 GCAGC GCAGC(8/12) BclI 86 TGATCA T^GATCA BmyI 8 GTGCCC G[AGT]GC[ACT]^C BsaI 659 GAGACC GGTCTC(1/5) BseRI 139 CTCCTC GAGGAG(10/8) BsiYI 161 CCAGAATTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 161 CCAGAATTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 799 GAGACG CGTCTC(1/5) Bsp1286I 8 GTGCCC G[AGT]GC[ACT]^C BsrDI 68 GCAATG GCAATG(2/0) BssKI 665 CCAGG ^CC[ACGT]GG BstNI 665 CCAGG CC^[AT]GG Cac8I 294 GCTTGC GC[ACGT]^[ACGT]GC DraI 426 TTTAAA TTT^AAA EcoRI 46 GAATTC G^AATTC EcoRII 665 CCAGG ^CC[AT]GG EcoT22I 448 ATGCAT ATGCA^T FbaI 86 TGATCA T^GATCA Fnu4HI 886 GCAGC GC^[ACGT]GC ItaI 886 GCAGC GC^[ACGT]GC MaeIII 509 GTGAC ^GT[ACGT]AC MvaI 665 CCAGG CC^[AT]GG NlaIV 406 GGAACC GG[ACGT]^[ACGT]CC NsiI 448 ATGCAT ATGCA^T PflFI 100 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 809 GAGTC GAGTC(4/5) PshAI 669 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 613 CTGCAG CTGCA^G RsaI 82 GTAC GT^AC ScaI 81 AGTACT AGT^ACT ScrFI 665 CCAGG CC^[ACGT]GG SfaNI 311 GCATC GCATC(5/9) SfcI 613 CTGCAG C^T[GA][CT]AG SspI 183 AATATT AAT^ATT StuI 869 AGGCCT AGG^CCT TaqI 131 TCGA T^CGA TseI 886 GCAGC G^C[AT]GC Tsp45I 509 GTGAC ^GT[GC]AC Tth111I 100 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 131 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BbsI 415 GTCTTC GAAGAC(2/6) 811 GTCTTC BpmI 159 CTCCAG CTGGAG(16/14) 625 CTCCAG BpuAI 415 GTCTTC GAAGAC(2/6) 811 GTCTTC BsmAI 659 GAGAC GTCTC(1/5) 799 GAGAC BsrI 101 ACTGG ACTGG(1/-1) 461 ACTGG EcoT14I 410 CCTTGG C^C[AT][AT]GG 671 CCAAGG HaeIII 579 GGCC GG^CC 870 GGCC MnlI 125 CCTC CCTC(7/6) 141 CCTC MslI 222 CATATTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 679 CATACAAATG NspI 446 ACATGC [GA]CATG^[CT] 788 ACATGC StyI 410 CCTTGG C^C[AT][AT]GG 671 CCAAGG TfiI 387 GATTC G^A[AT]TC 843 GATTC TspRI 527 CAGTG CA[GC]TG[ACGT][ACGT]^ 779 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AcsI AluI ApoI AvaII BfaI BsaJI Cfr13I DdeI DpnII HinfI MaeI MboI MboII MseI NdeII NlaIII Sau3AI Sau96I Tru9I Tsp509I