Restriction map for
pFN21AE5325
(Circular) (Six-base) MAPSORT of: pFN21AE5325.seq Check: 3977 from: 1 to: 5614
pFN21AE5325 5614 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3424 278
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2957 1937
Size: 1020 4594
Acc65I G'GTAC_C
Cuts at: 2935 2935
Size: 5614
AclI AA'CG_TT
Cuts at: 1563 3741 4114 1563
Size: 2178 373 3063
Fragments arranged by size:
3063 2178 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2296 3677 5061 5329 1632
Size: 664 1381 1384 268 1917
Fragments arranged by size:
1917 1384 1381 664 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5417
AflIII A'CryG_T
Cuts at: 1203 4513 5490 1203
Size: 3310 977 1327
Fragments arranged by size:
3310 1327 977
AgeI A'CCGG_T
Cuts at: 4445 4445
Size: 5614
AhdI GACnn_n'nnGTC
Cuts at: 4343 4343
Size: 5614
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5614
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5614
AlwNI CAG_nnn'CTG
Cuts at: 3357 4929 3357
Size: 1572 4042
ApaLI G'TGCA_C
Cuts at: 3671 4827 3671
Size: 1156 4458
ApoI r'AATT_y
Cuts at: 1493 2051 2065 2500 2543 2923 3149 3301 5410
Size: 558 14 435 43 380 226 152 2109
Cuts at: 5410 1493
Size: 1697
Fragments arranged by size:
2109 1697 558 435 380 226 152 43
14
AseI AT'TA_AT
Cuts at: 160 1032 4168 160
Size: 872 3136 1606
Fragments arranged by size:
3136 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
AvaI C'yCGr_G
Cuts at: 1870 1943 2939 1870
Size: 73 996 4545
Fragments arranged by size:
4545 996 73
BamHI G'GATC_C
Cuts at: 2944 3311 2944
Size: 367 5247
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2935 4390 618
Size: 325 217 627 1148 1455 1842
Fragments arranged by size:
1842 1455 1148 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2933 1391
Size: 1542 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2413 2809 928
Size: 971 514 396 3733
Fragments arranged by size:
3733 971 514 396
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3805 3839 1903
Size: 34 1868 34 3678
Fragments arranged by size:
3678 1868 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3506 4716 3506
Size: 1210 4404
BclI T'GATC_A
Cuts at: 1548 2091 1548
Size: 543 5071
BfrBI ATG'CAT
Cuts at: 2455 2455
Size: 5614
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4225 136
Size: 107 122 71 631 605 2553 1525
Fragments arranged by size:
2553 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5609 5609
Size: 5614
BlpI GC'TnA_GC
Cuts at: 3031 3031
Size: 5614
Bme1580I G_kGCm'C
Cuts at: 1790 2017 3675 4831 1790
Size: 227 1658 1156 2573
Fragments arranged by size:
2573 1658 1156 227
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5614
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2116 3345 4303
Size: 416 53 83 399 24 699 1229 958
Cuts at: 4303 442
Size: 1753
Fragments arranged by size:
1753 1229 958 699 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5614
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2146 2146
Size: 5614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2149 2615 4274 1239
Size: 910 466 1659 2579
Fragments arranged by size:
2579 1659 910 466
Bpu10I CC'TnA_GC
Cuts at: 2895 2895
Size: 5614
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3739 4604 4902 3739
Size: 865 298 4451
Fragments arranged by size:
4451 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2658 4277 882
Size: 617 1159 1619 2219
Fragments arranged by size:
2219 1619 1159 617
BsaAI yAC'GTr
Cuts at: 493 1204 3405 493
Size: 711 2201 2702
Fragments arranged by size:
2702 2201 711
BsaBI GATnn'nnATC
Cuts at: 5608 5608
Size: 5614
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3421 3803 4438 275
Size: 53 83 186 936 1888 382 635 1451
Fragments arranged by size:
1888 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4047 4445 4719 4866 5277 4047
Size: 398 274 147 411 4384
Fragments arranged by size:
4384 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5389
Fragments arranged by size:
5389 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 1186
Size: 949 4665
BseYI C'CCAG_C
Cuts at: 4817 5313 4817
Size: 496 5118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5041
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3064 3825 4853 1540
Size: 275 186 1063 761 1028 2301
Fragments arranged by size:
2301 1063 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3675 3760 4831 3675
Size: 85 1071 4458
Fragments arranged by size:
4458 1071 85
BsmI GAATG_Cn'
Cuts at: 3125 3218 3125
Size: 93 5521
BsmBI CGTCTCn'nnnn_
Cuts at: 2798 2798
Size: 5614
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2017 2933 3675 3760 4831 1391
Size: 399 227 916 742 85 1071 2174
Fragments arranged by size:
2174 1071 916 742 399 227 85
BspEI T'CCGG_A
Cuts at: 5277 5277
Size: 5614
BspHI T'CATG_A
Cuts at: 3503 5233 3503
Size: 1730 3884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2969 844
Size: 2125 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3501 1394
Size: 429 1678 3507
Fragments arranged by size:
3507 1678 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2080 4109 4283 65
Size: 1183 832 2029 174 1396
Fragments arranged by size:
2029 1396 1183 832 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4258 4445 1700
Size: 110 2448 187 2869
Fragments arranged by size:
2869 2448 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5614
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5614
BssSI C'ACGA_G
Cuts at: 3674 4686 3674
Size: 1012 4602
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5614
BstBI TT'CG_AA
Cuts at: 4418 4418
Size: 5614
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5614
BstYI r'GATC_y
Cuts at: 1972 2944 3311 3696 3713 5165 5251 5263 5609
Size: 972 367 385 17 1452 86 12 346
Cuts at: 5609 1972
Size: 1977
Fragments arranged by size:
1977 1452 972 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4123
Fragments arranged by size:
4123 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4875
Fragments arranged by size:
4875 683 56
BtsI GCAGTG_nn'
Cuts at: 3130 3924 3944 3130
Size: 794 20 4800
Fragments arranged by size:
4800 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5614
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5579
DraI TTT'AAA
Cuts at: 2433 2918 2433
Size: 485 5129
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4621 809
Size: 507 3305 1802
Fragments arranged by size:
3305 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3061 3950 5579
Size: 54 1425 50 275 18 1231 889 1629
Cuts at: 5579 8
Size: 43
Fragments arranged by size:
1629 1425 1231 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3061 1537
Size: 275 1249 4090
Fragments arranged by size:
4090 1249 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3544 1353
Size: 2191 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4201 4575 4721 387
Size: 1064 138 2612 374 146 1280
Fragments arranged by size:
2612 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2149 2296 2615 3677 4274 5061 5329
Size: 393 517 147 319 1062 597 787 268
Cuts at: 5329 1239
Size: 1524
Fragments arranged by size:
1524 1062 787 597 517 393 319 268
147
EcoRI G'AATT_C
Cuts at: 2051 2923 3301 2051
Size: 872 378 4364
Fragments arranged by size:
4364 872 378
FspI TGC'GCA
Cuts at: 4120 4120
Size: 5614
HaeII r_GCGC'y
Cuts at: 1678 4761 1678
Size: 3083 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4255 4287 4329 4361 1373
Size: 32 135 32 2683 32 42 32 2626
Fragments arranged by size:
2683 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2958 3204 669
Size: 988 281 1020 246 3079
Fragments arranged by size:
3079 1020 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5614
HpaI GTT'AAC
Cuts at: 3204 3204
Size: 5614
KpnI G_GTAC'C
Cuts at: 2939 2939
Size: 5614
MfeI C'AATT_G
Cuts at: 3213 3213
Size: 5614
MluI A'CGCG_T
Cuts at: 5490 5490
Size: 5614
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4728 4912 1305
Size: 3423 184 2007
Fragments arranged by size:
3423 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3792
Fragments arranged by size:
3792 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 2688 3572 3931 4090 518
Size: 648 1065 457 884 359 159 2042
Fragments arranged by size:
2042 1065 884 648 457 359 159
MspA1I CmG'CkG
Cuts at: 1606 3030 3707 4855 5100 1606
Size: 1424 677 1148 245 2120
Fragments arranged by size:
2120 1424 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4123
Fragments arranged by size:
4123 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5614
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5614
NotI GC'GGCC_GC
Cuts at: 3061 3061
Size: 5614
NsiI A_TGCA'T
Cuts at: 2457 2457
Size: 5614
NspI r_CATG'y
Cuts at: 1132 1229 2455 2797 2972 4517 1132
Size: 97 1226 342 175 1545 2229
Fragments arranged by size:
2229 1545 1226 342 175 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5614
PciI A'CATG_T
Cuts at: 4513 4513
Size: 5614
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5614
PmeI GTTT'AAAC
Cuts at: 2918 2918
Size: 5614
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3683 3715 5196 5228 3683
Size: 32 1481 32 4069
Fragments arranged by size:
4069 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2678 1613
Size: 1065 4549
PsiI TTA'TAA
Cuts at: 3184 3184
Size: 5614
PstI C_TGCA'G
Cuts at: 830 2622 2966 830
Size: 1792 344 3478
Fragments arranged by size:
3478 1792 344
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5614
SalI G'TCGA_C
Cuts at: 1936 2956 1936
Size: 1020 4594
SbfI CC_TGCA'GG
Cuts at: 2966 2966
Size: 5614
ScaI AGT'ACT
Cuts at: 2088 3862 5574 2088
Size: 1774 1712 2128
Fragments arranged by size:
2128 1774 1712
SfcI C'TryA_G
Cuts at: 826 1045 2618 2962 4097 4778 4969 826
Size: 219 1573 344 1135 681 191 1471
Fragments arranged by size:
1573 1471 1135 681 344 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5614
SmaI CCC'GGG
Cuts at: 1872 2941 1872
Size: 1069 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3718 4619 4881 820
Size: 197 926 1775 901 262 1553
Fragments arranged by size:
1775 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3405 493
Size: 2912 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5614
SphI G_CATG'C
Cuts at: 1132 2972 1132
Size: 1840 3774
SspI AAT'ATT
Cuts at: 5 52 2190 3538 5
Size: 47 2138 1348 2081
Fragments arranged by size:
2138 2081 1348 47
StuI AGG'CCT
Cuts at: 1517 2876 1517
Size: 1359 4255
StyI C'CwwG_G
Cuts at: 513 1065 2004 2415 2676 3053 513
Size: 552 939 411 261 377 3074
Fragments arranged by size:
3074 939 552 411 377 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2490 2527 3641 3658 3811 3996 4441
Size: 1255 910 37 1114 17 153 185 445
Cuts at: 4441 325
Size: 1498
Fragments arranged by size:
1498 1255 1114 910 445 185 153 37
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2086 3860 5572
Size: 274 80 33 51 1443 109 1774 1712
Cuts at: 5572 96
Size: 138
Fragments arranged by size:
1774 1712 1443 274 138 109 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5574 5606 5574
Size: 32 5582
Tth111I GACn'n_nGTC
Cuts at: 1316 2108 1316
Size: 792 4822
XbaI T'CTAG_A
Cuts at: 2950 2950
Size: 5614
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5614
XmaI C'CCGG_G
Cuts at: 1870 2939 1870
Size: 1069 4545
XmnI GAAnn'nnTTC
Cuts at: 3743 3743
Size: 5614
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3422 276
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI