Restriction map for
pFN21AE5383
##################################
# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AvaI
AvaII AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BmyI BpmI BpuAI
BsaBI BsaHI BsaI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspMI BsrBI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstUI BstXI BstZ17I Bsu36I CfoI
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoRI EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MluI MroI MslI MspA1I MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AfaI 871 GTAC GT^AC
AlwI 486 GGATC GGATC(4/5)
AspI 475 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 728 TGGCCA TGG^CCA
BbvCI 303 CCTCAGC CCTCAGC(-5/-2)
BfaI 835 CTAG C^TAG
BlpI 146 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 146 GCTTAGC GC^T[ACGT]AGC
BsaAI 5 TACGTG [CT]AC^GT[GA]
BsaWI 229 TCCGGT [AT]^CCGG[AT]
BsiYI 860 CCTCTTTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 860 CCTCTTTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspHI 392 TCATGA T^CATGA
BsrFI 142 ACCGGC [GA]^CCGG[CT]
BssKI 809 CCTGG ^CC[ACGT]GG
BstAPI 433 GCATACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 232 GGTAACC G^GT[ACGT]ACC
BstF5I 699 CATCC GGATG(2/0)
BstNI 809 CCTGG CC^[AT]GG
BstPI 232 GGTAACC G^GT[ACGT]ACC
BstYI 486 GGATCT [GA]^GATC[CT]
CelII 146 GCTTAGC GC^T[ACGT]AGC
Cfr10I 142 ACCGGC [GA]^CCGG[CT]
DpnII 487 GATC ^GATC
EaeI 728 TGGCCA [CT]^GGCC[GA]
EarI 363 GAAGAG CTCTTC(1/4)
EcoNI 860 CCTCTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 232 GGTAACC G^GT[ACGT]ACC
EcoRII 809 CCTGG ^CC[AT]GG
EcoRV 687 GATATC GAT^ATC
FokI 699 CATCC GGATG(9/13)
HaeIII 729 GGCC GG^CC
HphI 140 TCACC GGTGA(8/7)
Ksp632I 363 GAAGAG CTCTTC(1/4)
MaeI 835 CTAG C^TAG
MaeII 6 ACGT A^CGT
MboI 487 GATC ^GATC
MfeI 880 CAATTG C^AATTG
MflI 486 GGATCT [GA]^GATC[CT]
MluNI 728 TGGCCA TGG^CCA
MscI 728 TGGCCA TGG^CCA
MunI 880 CAATTG C^AATTG
MvaI 809 CCTGG CC^[AT]GG
MwoI 433 GCATACATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 487 GATC ^GATC
PflFI 475 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 874 CTGCAG CTGCA^G
RcaI 392 TCATGA T^CATGA
RsaI 871 GTAC GT^AC
SapI 363 GAAGAGC GCTCTTC(1/4)
Sau3AI 487 GATC ^GATC
ScrFI 809 CCTGG CC^[ACGT]GG
SfcI 874 CTGCAG C^T[GA][CT]AG
TaqI 625 TCGA T^CGA
TfiI 447 GATTC G^A[AT]TC
TspRI 50 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 475 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 625 TCGA T^CGA
XhoII 486 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcsI 161 GAATTT [GA]^AATT[CT]
333 AAATTT
ApoI 161 GAATTT [GA]^AATT[CT]
333 AAATTT
BbvI 876 GCAGC GCAGC(8/12)
771 GCTGC
BsaJI 808 CCCTGG C^C[ACGT][ACGT]GG
809 CCTGGG
BsmAI 603 GAGAC GTCTC(1/5)
644 GAGAC
BspLU11I 494 ACATGT A^CATGT
574 ACATGT
BsrDI 438 CATTGC GCAATG(2/0)
673 GCAATG
Cac8I 845 GCAAGC GC[ACGT]^[ACGT]GC
855 GCAAGC
Fnu4HI 771 GCTGC GC^[ACGT]GC
876 GCAGC
HapII 143 CCGG C^CGG
230 CCGG
HpaII 143 CCGG C^CGG
230 CCGG
ItaI 771 GCTGC GC^[ACGT]GC
876 GCAGC
MspI 143 CCGG C^CGG
230 CCGG
NspI 494 ACATGT [GA]CATG^[CT]
574 ACATGT
TseI 771 GCTGC G^C[AT]GC
876 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AflIII AluI DdeI HinfI MaeIII
MboII MnlI MseI NlaIII PleI
SfaNI Tru9I Tsp45I Tsp509I