Restriction map for pFN21AE4387
 (Circular) (Six-base) MAPSORT of: pFN21AE4387.seq  Check: 2862  from: 1  to: 5929



pFN21AE4387  5929 bp

 With 182 enzymes: SgfI * 

                        March 28, 2012 14:31  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3739     278 
   Size:         53      83     186     936    2203    2468

  Fragments arranged by size: 

                2468    2203     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2517    3272    1937 
   Size:        580     755    4594

  Fragments arranged by size: 

                4594     755     580
 
Acc65I G'GTAC_C

Cuts at:   2733    3250    2733 
   Size:        517    5412
 
AclI AA'CG_TT

Cuts at:   1563    4056    4429    1563 
   Size:       2493     373    3063

  Fragments arranged by size: 

                3063    2493     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2800    3075    3992    5376    5644    1632 
   Size:       1168     275     917    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1168     917     275     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5732
 
AflIII A'CryG_T

Cuts at:   1203    4828    5805    1203 
   Size:       3625     977    1327

  Fragments arranged by size: 

                3625    1327     977
 
AgeI A'CCGG_T

Cuts at:   4760    4760 
   Size:       5929
 
AhdI GACnn_n'nnGTC

Cuts at:   4658    4658 
   Size:       5929
 
AleI CACnn'nnGTG

Cuts at:   1166    3045    1166 
   Size:       1879    4050
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2843    1184 
   Size:       1659    4270
 
AlwNI CAG_nnn'CTG

Cuts at:   2884    3169    3672    5244    2884 
   Size:        285     503    1572    3569

  Fragments arranged by size: 

                3569    1572     503     285
 
ApaI G_GGCC'C

Cuts at:   3123    3123 
   Size:       5929
 
ApaLI G'TGCA_C

Cuts at:   3986    5142    3986 
   Size:       1156    4773
 
ApoI r'AATT_y

Cuts at:   1493    2285    3065    3238    3464    3616    5725    1493 
   Size:        792     780     173     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     792     780     226     173     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4483     160 
   Size:        872    3451    1606

  Fragments arranged by size: 

                3451    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5929
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3254    1870 
   Size:         73    1311    4545

  Fragments arranged by size: 

                4545    1311      73
 
BamHI G'GATC_C

Cuts at:   3259    3626    3259 
   Size:        367    5562
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2303    2370    2455    2733    2993 
   Size:        325     217     627     516      67      85     278     260

Cuts at:   2993    3250    4705     618 
   Size:        257    1455    1842

  Fragments arranged by size: 

                1842    1455     627     516     325     278     260     257
                 217      85      67
 
BanII G_rGCy'C

Cuts at:   1391    3123    3248    1391 
   Size:       1732     125    4072

  Fragments arranged by size: 

                4072    1732     125
 
BbeI G_GCGC'C

Cuts at:   2374    2374 
   Size:       5929
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2617     928 
   Size:        971     718    4240

  Fragments arranged by size: 

                4240     971     718
 
BbvCI CC'TCA_GC

Cuts at:   2877    2877 
   Size:       5929
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4120    4154    1903 
   Size:         34    2183      34    3678

  Fragments arranged by size: 

                3678    2183      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3821    5031    3821 
   Size:       1210    4719
 
BclI T'GATC_A

Cuts at:   1548    2040    2184    2662    1548 
   Size:        492     144     478    4815

  Fragments arranged by size: 

                4815     492     478     144
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    4540     136 
   Size:        107     122      71     631     605     337    2531    1525

  Fragments arranged by size: 

                2531    1525     631     605     337     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5924    5924 
   Size:       5929
 
BlpI GC'TnA_GC

Cuts at:   3346    3346 
   Size:       5929
 
Bme1580I G_kGCm'C

Cuts at:   1790    2308    2460    2996    3123    3990    5146    1790 
   Size:        518     152     536     127     867    1156    2573

  Fragments arranged by size: 

                2573    1156     867     536     518     152     127
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5929
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3660    4618     442 
   Size:        416      53      83     399      24    2243     958    1753

  Fragments arranged by size: 

                2243    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5929
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2966    3008    4589    1239 
   Size:       1727      42    1581    2579

  Fragments arranged by size: 

                2579    1727    1581      42
 
Bpu10I CC'TnA_GC

Cuts at:   2877    2877 
   Size:       5929
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4054    4919    5217    4054 
   Size:        865     298    4766

  Fragments arranged by size: 

                4766     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4592     882 
   Size:        617    3093    2219

  Fragments arranged by size: 

                3093    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3720     493 
   Size:        711    2516    2702

  Fragments arranged by size: 

                2702    2516     711
 
BsaBI GATnn'nnATC

Cuts at:   5923    5923 
   Size:       5929
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2371    3736    4118    4753 
   Size:         53      83     186     936     838    1365     382     635

Cuts at:   4753     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1365     936     838     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   4362    4760    5034    5181    5592    4362 
   Size:        398     274     147     411    4699

  Fragments arranged by size: 

                4699     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2394    2424    1178 
   Size:         30     165      30     991      30    4683

  Fragments arranged by size: 

                4683     991     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2402    1186 
   Size:       1216    4713
 
BseYI C'CCAG_C

Cuts at:   2062    5132    5628    2062 
   Size:       3070     496    2363

  Fragments arranged by size: 

                3070    2363     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5356
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3379    4140    5168    1540 
   Size:        275     186    1378     761    1028    2301

  Fragments arranged by size: 

                2301    1378    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3990    4075    5146    3990 
   Size:         85    1071    4773

  Fragments arranged by size: 

                4773    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2270    3440    3533    2270 
   Size:       1170      93    4666

  Fragments arranged by size: 

                4666    1170      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2059    2059 
   Size:       5929
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2308    2460    2996    3123    3248    3990    4075 
   Size:        399     518     152     536     127     125     742      85

Cuts at:   4075    5146    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     536     518     399     152     127
                 125      85
 
BspEI T'CCGG_A

Cuts at:   5592    5592 
   Size:       5929
 
BspHI T'CATG_A

Cuts at:   2685    3818    5548    2685 
   Size:       1133    1730    3066

  Fragments arranged by size: 

                3066    1730    1133
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3284     844 
   Size:       2440    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3816    1394 
   Size:        429    1993    3507

  Fragments arranged by size: 

                3507    1993     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2112    3143    4424    4598      65 
   Size:       1183     864    1031    1281     174    1396

  Fragments arranged by size: 

                1396    1281    1183    1031     864     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4573    4760    1700 
   Size:        110    2763     187    2869

  Fragments arranged by size: 

                2869    2763     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5929
 
BssHII G'CGCG_C

Cuts at:   1925    2120    1925 
   Size:        195    5734
 
BssSI C'ACGA_G

Cuts at:   3989    5001    3989 
   Size:       1012    4917
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5929
 
BstBI TT'CG_AA

Cuts at:   4733    4733 
   Size:       5929
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5929
 
BstYI r'GATC_y

Cuts at:   1972    2163    3165    3259    3626    4011    4028    5480    5566 
   Size:        191    1002      94     367     385      17    1452      86

Cuts at:   5566    5578    5924    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452    1002     385     367     346     191      94
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2793     513 
   Size:        552    1728    3649

  Fragments arranged by size: 

                3649    1728     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5190

  Fragments arranged by size: 

                5190     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2267    2541    2870    3181    3445    4239    4259    2267 
   Size:        274     329     311     264     794      20    3937

  Fragments arranged by size: 

                3937     794     329     311     274     264      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5929
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5894
 
DraI TTT'AAA

Cuts at:   3006    3233    3006 
   Size:        227    5702
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4936     809 
   Size:        507    3620    1802

  Fragments arranged by size: 

                3620    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2073    2590    3376 
   Size:         54    1425      50     275      18     243     517     786

Cuts at:   3376    4265    5894       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     786     517     275     243      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3376    1537 
   Size:        275    1564    4090

  Fragments arranged by size: 

                4090    1564     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2219    3221    3859    1353 
   Size:        866    1002     638    3423

  Fragments arranged by size: 

                3423    1002     866     638
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2459    4516    4890    5036     387 
   Size:       1064     138     870    2057     374     146    1280

  Fragments arranged by size: 

                2057    1280    1064     870     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2800    2966    3008    3075    3992    4589    5376 
   Size:        393    1168     166      42      67     917     597     787

Cuts at:   5376    5644    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1168     917     787     597     393     268     166
                  67      42
 
EcoO109I rG'GnC_Cy

Cuts at:   2137    2137 
   Size:       5929
 
EcoRI G'AATT_C

Cuts at:   3065    3238    3616    3065 
   Size:        173     378    5378

  Fragments arranged by size: 

                5378     378     173
 
EcoRV GAT'ATC

Cuts at:   3200    3200 
   Size:       5929
 
FspI TGC'GCA

Cuts at:   4435    4435 
   Size:       5929
 
HaeII r_GCGC'y

Cuts at:   1678    2374    5076    1678 
   Size:        696    2702    2531

  Fragments arranged by size: 

                2702    2531     696
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2148    2180    2913    2945    3189 
   Size:         32     135      32     576      32     733      32     244

Cuts at:   3189    3221    4570    4602    4644    4676    1373 
   Size:         32    1349      32      42      32    2626

  Fragments arranged by size: 

                2626    1349     733     576     244     135      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3273    3519     669 
   Size:        988     281    1335     246    3079

  Fragments arranged by size: 

                3079    1335     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5929
 
HpaI GTT'AAC

Cuts at:   3519    3519 
   Size:       5929
 
KasI G'GCGC_C

Cuts at:   2370    2370 
   Size:       5929
 
KpnI G_GTAC'C

Cuts at:   2737    3254    2737 
   Size:        517    5412
 
MfeI C'AATT_G

Cuts at:   3528    3528 
   Size:       5929
 
MluI A'CGCG_T

Cuts at:   5805    5805 
   Size:       5929
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2622    5043    5227    1305 
   Size:       1317    2421     184    2007

  Fragments arranged by size: 

                2421    2007    1317     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2075    2592      10 
   Size:         54    1425     343     243     517    3347

  Fragments arranged by size: 

                3347    1425     517     343     243      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2453    3045    3887    4246    4405     518 
   Size:        648    1287     592     842     359     159    2042

  Fragments arranged by size: 

                2042    1287     842     648     592     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2035    2190    2396    3028    3345    4022    5170    5415 
   Size:        429     155     206     632     317     677    1148     245

Cuts at:   5415    1606 
   Size:       2120

  Fragments arranged by size: 

                2120    1148     677     632     429     317     245     206
                 155
 
NarI GG'CG_CC

Cuts at:   2371    2371 
   Size:       5929
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5377
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5929
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5929
 
NotI GC'GGCC_GC

Cuts at:   3376    3376 
   Size:       5929
 
NspI r_CATG'y

Cuts at:   1132    1229    3287    4832    1132 
   Size:         97    2058    1545    2229

  Fragments arranged by size: 

                2229    2058    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    2176    1348 
   Size:        828    5101
 
PciI A'CATG_T

Cuts at:   4828    4828 
   Size:       5929
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5929
 
PmeI GTTT'AAAC

Cuts at:   3233    3233 
   Size:       5929
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3998    4030    5511    5543    3998 
   Size:         32    1481      32    4384

  Fragments arranged by size: 

                4384    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2137    2137 
   Size:       5929
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5929
 
PsiI TTA'TAA

Cuts at:   3499    3499 
   Size:       5929
 
PspOMI G'GGCC_C

Cuts at:   3119    3119 
   Size:       5929
 
PstI C_TGCA'G

Cuts at:    830    3165    3281     830 
   Size:       2335     116    3478

  Fragments arranged by size: 

                3478    2335     116
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5929
 
PvuII CAG'CTG

Cuts at:   2396    3028    2396 
   Size:        632    5297
 
SalI G'TCGA_C

Cuts at:   1936    3271    1936 
   Size:       1335    4594
 
SbfI CC_TGCA'GG

Cuts at:   3281    3281 
   Size:       5929
 
ScaI AGT'ACT

Cuts at:   4177    5889    4177 
   Size:       1712    4217
 
SexAI A'CCwGG_T

Cuts at:   2631    2631 
   Size:       5929
 
SfcI C'TryA_G

Cuts at:    826    1045    2391    3161    3277    4412    5093    5284     826 
   Size:        219    1346     770     116    1135     681     191    1471

  Fragments arranged by size: 

                1471    1346    1135     770     681     219     191     116
            
 
SfoI GGC'GCC

Cuts at:   2372    2372 
   Size:       5929
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5929
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5929
 
SmaI CCC'GGG

Cuts at:   1872    3256    1872 
   Size:       1384    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    4033    4934    5196     820 
   Size:        197     926    2090     901     262    1553

  Fragments arranged by size: 

                2090    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3720     493 
   Size:       3227    2702
 
SpeI A'CTAG_T

Cuts at:    152    2599     152 
   Size:       2447    3482
 
SphI G_CATG'C

Cuts at:   1132    3287    1132 
   Size:       2155    3774
 
SspI AAT'ATT

Cuts at:      5      52    3853       5 
   Size:         47    3801    2081

  Fragments arranged by size: 

                3801    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5929
 
StyI C'CwwG_G

Cuts at:    513    1065    3115    3368     513 
   Size:        552    2050     253    3074

  Fragments arranged by size: 

                3074    2050     552     253
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3956    3973    4126    4311    4756     325 
   Size:       1255    2376      17     153     185     445    1498

  Fragments arranged by size: 

                2376    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2353    2508    3037 
   Size:        274      80      33      51    1443     376     155     529

Cuts at:   3037    4175    5887      96 
   Size:       1138    1712     138

  Fragments arranged by size: 

                1712    1443    1138     529     376     274     155     138
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2394    2426    5889    5921    2394 
   Size:         32    3463      32    2402

  Fragments arranged by size: 

                3463    2402      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5929
 
XbaI T'CTAG_A

Cuts at:   3265    3265 
   Size:       5929
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5929
 
XmaI C'CCGG_G

Cuts at:   1870    3254    1870 
   Size:       1384    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4058    4058 
   Size:       5929
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3737     276 
   Size:         53      83     186     936    2203    2468

  Fragments arranged by size: 

                2468    2203     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRI    EcoRV     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI
    PpuMI    PshAI     PsiI   PspOMI     PstI     PvuI    PvuII     SalI
     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI    BfrBI     BplI
    BsiWI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoNI     FalI
     FseI    FspAI     NaeI   NgoMIV     NruI     NsiI     PacI     PfoI
     PmlI    PspXI     PsrI    RsrII     SacI    SacII    SanDI     SapI
     SfiI     SrfI     SwaI     XcmI
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