(Circular) (Six-base) MAPSORT of: pFN21AE4387.seq Check: 2862 from: 1 to: 5929 pFN21AE4387 5929 bp With 182 enzymes: SgfI * March 28, 2012 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3739 278 Size: 53 83 186 936 2203 2468 Fragments arranged by size: 2468 2203 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2517 3272 1937 Size: 580 755 4594 Fragments arranged by size: 4594 755 580 Acc65I G'GTAC_C Cuts at: 2733 3250 2733 Size: 517 5412 AclI AA'CG_TT Cuts at: 1563 4056 4429 1563 Size: 2493 373 3063 Fragments arranged by size: 3063 2493 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2800 3075 3992 5376 5644 1632 Size: 1168 275 917 1384 268 1917 Fragments arranged by size: 1917 1384 1168 917 275 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5732 AflIII A'CryG_T Cuts at: 1203 4828 5805 1203 Size: 3625 977 1327 Fragments arranged by size: 3625 1327 977 AgeI A'CCGG_T Cuts at: 4760 4760 Size: 5929 AhdI GACnn_n'nnGTC Cuts at: 4658 4658 Size: 5929 AleI CACnn'nnGTG Cuts at: 1166 3045 1166 Size: 1879 4050 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2843 1184 Size: 1659 4270 AlwNI CAG_nnn'CTG Cuts at: 2884 3169 3672 5244 2884 Size: 285 503 1572 3569 Fragments arranged by size: 3569 1572 503 285 ApaI G_GGCC'C Cuts at: 3123 3123 Size: 5929 ApaLI G'TGCA_C Cuts at: 3986 5142 3986 Size: 1156 4773 ApoI r'AATT_y Cuts at: 1493 2285 3065 3238 3464 3616 5725 1493 Size: 792 780 173 226 152 2109 1697 Fragments arranged by size: 2109 1697 792 780 226 173 152 AseI AT'TA_AT Cuts at: 160 1032 4483 160 Size: 872 3451 1606 Fragments arranged by size: 3451 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5929 AvaI C'yCGr_G Cuts at: 1870 1943 3254 1870 Size: 73 1311 4545 Fragments arranged by size: 4545 1311 73 BamHI G'GATC_C Cuts at: 3259 3626 3259 Size: 367 5562 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2303 2370 2455 2733 2993 Size: 325 217 627 516 67 85 278 260 Cuts at: 2993 3250 4705 618 Size: 257 1455 1842 Fragments arranged by size: 1842 1455 627 516 325 278 260 257 217 85 67 BanII G_rGCy'C Cuts at: 1391 3123 3248 1391 Size: 1732 125 4072 Fragments arranged by size: 4072 1732 125 BbeI G_GCGC'C Cuts at: 2374 2374 Size: 5929 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2617 928 Size: 971 718 4240 Fragments arranged by size: 4240 971 718 BbvCI CC'TCA_GC Cuts at: 2877 2877 Size: 5929 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4120 4154 1903 Size: 34 2183 34 3678 Fragments arranged by size: 3678 2183 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3821 5031 3821 Size: 1210 4719 BclI T'GATC_A Cuts at: 1548 2040 2184 2662 1548 Size: 492 144 478 4815 Fragments arranged by size: 4815 492 478 144 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4540 136 Size: 107 122 71 631 605 337 2531 1525 Fragments arranged by size: 2531 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5924 5924 Size: 5929 BlpI GC'TnA_GC Cuts at: 3346 3346 Size: 5929 Bme1580I G_kGCm'C Cuts at: 1790 2308 2460 2996 3123 3990 5146 1790 Size: 518 152 536 127 867 1156 2573 Fragments arranged by size: 2573 1156 867 536 518 152 127 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5929 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3660 4618 442 Size: 416 53 83 399 24 2243 958 1753 Fragments arranged by size: 2243 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5929 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2966 3008 4589 1239 Size: 1727 42 1581 2579 Fragments arranged by size: 2579 1727 1581 42 Bpu10I CC'TnA_GC Cuts at: 2877 2877 Size: 5929 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4054 4919 5217 4054 Size: 865 298 4766 Fragments arranged by size: 4766 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4592 882 Size: 617 3093 2219 Fragments arranged by size: 3093 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3720 493 Size: 711 2516 2702 Fragments arranged by size: 2702 2516 711 BsaBI GATnn'nnATC Cuts at: 5923 5923 Size: 5929 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2371 3736 4118 4753 Size: 53 83 186 936 838 1365 382 635 Cuts at: 4753 275 Size: 1451 Fragments arranged by size: 1451 1365 936 838 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4362 4760 5034 5181 5592 4362 Size: 398 274 147 411 4699 Fragments arranged by size: 4699 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2394 2424 1178 Size: 30 165 30 991 30 4683 Fragments arranged by size: 4683 991 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2402 1186 Size: 1216 4713 BseYI C'CCAG_C Cuts at: 2062 5132 5628 2062 Size: 3070 496 2363 Fragments arranged by size: 3070 2363 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5356 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3379 4140 5168 1540 Size: 275 186 1378 761 1028 2301 Fragments arranged by size: 2301 1378 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3990 4075 5146 3990 Size: 85 1071 4773 Fragments arranged by size: 4773 1071 85 BsmI GAATG_Cn' Cuts at: 2270 3440 3533 2270 Size: 1170 93 4666 Fragments arranged by size: 4666 1170 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2059 2059 Size: 5929 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2308 2460 2996 3123 3248 3990 4075 Size: 399 518 152 536 127 125 742 85 Cuts at: 4075 5146 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 536 518 399 152 127 125 85 BspEI T'CCGG_A Cuts at: 5592 5592 Size: 5929 BspHI T'CATG_A Cuts at: 2685 3818 5548 2685 Size: 1133 1730 3066 Fragments arranged by size: 3066 1730 1133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3284 844 Size: 2440 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3816 1394 Size: 429 1993 3507 Fragments arranged by size: 3507 1993 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2112 3143 4424 4598 65 Size: 1183 864 1031 1281 174 1396 Fragments arranged by size: 1396 1281 1183 1031 864 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4573 4760 1700 Size: 110 2763 187 2869 Fragments arranged by size: 2869 2763 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5929 BssHII G'CGCG_C Cuts at: 1925 2120 1925 Size: 195 5734 BssSI C'ACGA_G Cuts at: 3989 5001 3989 Size: 1012 4917 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5929 BstBI TT'CG_AA Cuts at: 4733 4733 Size: 5929 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5929 BstYI r'GATC_y Cuts at: 1972 2163 3165 3259 3626 4011 4028 5480 5566 Size: 191 1002 94 367 385 17 1452 86 Cuts at: 5566 5578 5924 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1002 385 367 346 191 94 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2793 513 Size: 552 1728 3649 Fragments arranged by size: 3649 1728 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5190 Fragments arranged by size: 5190 683 56 BtsI GCAGTG_nn' Cuts at: 2267 2541 2870 3181 3445 4239 4259 2267 Size: 274 329 311 264 794 20 3937 Fragments arranged by size: 3937 794 329 311 274 264 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5929 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5894 DraI TTT'AAA Cuts at: 3006 3233 3006 Size: 227 5702 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4936 809 Size: 507 3620 1802 Fragments arranged by size: 3620 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2590 3376 Size: 54 1425 50 275 18 243 517 786 Cuts at: 3376 4265 5894 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 786 517 275 243 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3376 1537 Size: 275 1564 4090 Fragments arranged by size: 4090 1564 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2219 3221 3859 1353 Size: 866 1002 638 3423 Fragments arranged by size: 3423 1002 866 638 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2459 4516 4890 5036 387 Size: 1064 138 870 2057 374 146 1280 Fragments arranged by size: 2057 1280 1064 870 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2800 2966 3008 3075 3992 4589 5376 Size: 393 1168 166 42 67 917 597 787 Cuts at: 5376 5644 1239 Size: 268 1524 Fragments arranged by size: 1524 1168 917 787 597 393 268 166 67 42 EcoO109I rG'GnC_Cy Cuts at: 2137 2137 Size: 5929 EcoRI G'AATT_C Cuts at: 3065 3238 3616 3065 Size: 173 378 5378 Fragments arranged by size: 5378 378 173 EcoRV GAT'ATC Cuts at: 3200 3200 Size: 5929 FspI TGC'GCA Cuts at: 4435 4435 Size: 5929 HaeII r_GCGC'y Cuts at: 1678 2374 5076 1678 Size: 696 2702 2531 Fragments arranged by size: 2702 2531 696 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2148 2180 2913 2945 3189 Size: 32 135 32 576 32 733 32 244 Cuts at: 3189 3221 4570 4602 4644 4676 1373 Size: 32 1349 32 42 32 2626 Fragments arranged by size: 2626 1349 733 576 244 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3273 3519 669 Size: 988 281 1335 246 3079 Fragments arranged by size: 3079 1335 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5929 HpaI GTT'AAC Cuts at: 3519 3519 Size: 5929 KasI G'GCGC_C Cuts at: 2370 2370 Size: 5929 KpnI G_GTAC'C Cuts at: 2737 3254 2737 Size: 517 5412 MfeI C'AATT_G Cuts at: 3528 3528 Size: 5929 MluI A'CGCG_T Cuts at: 5805 5805 Size: 5929 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2622 5043 5227 1305 Size: 1317 2421 184 2007 Fragments arranged by size: 2421 2007 1317 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2075 2592 10 Size: 54 1425 343 243 517 3347 Fragments arranged by size: 3347 1425 517 343 243 54 MslI CAynn'nnrTG Cuts at: 518 1166 2453 3045 3887 4246 4405 518 Size: 648 1287 592 842 359 159 2042 Fragments arranged by size: 2042 1287 842 648 592 359 159 MspA1I CmG'CkG Cuts at: 1606 2035 2190 2396 3028 3345 4022 5170 5415 Size: 429 155 206 632 317 677 1148 245 Cuts at: 5415 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 632 429 317 245 206 155 NarI GG'CG_CC Cuts at: 2371 2371 Size: 5929 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5377 NdeI CA'TA_TG Cuts at: 387 387 Size: 5929 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5929 NotI GC'GGCC_GC Cuts at: 3376 3376 Size: 5929 NspI r_CATG'y Cuts at: 1132 1229 3287 4832 1132 Size: 97 2058 1545 2229 Fragments arranged by size: 2229 2058 1545 97 PasI CC'CwG_GG Cuts at: 1348 2176 1348 Size: 828 5101 PciI A'CATG_T Cuts at: 4828 4828 Size: 5929 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5929 PmeI GTTT'AAAC Cuts at: 3233 3233 Size: 5929 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3998 4030 5511 5543 3998 Size: 32 1481 32 4384 Fragments arranged by size: 4384 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2137 2137 Size: 5929 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5929 PsiI TTA'TAA Cuts at: 3499 3499 Size: 5929 PspOMI G'GGCC_C Cuts at: 3119 3119 Size: 5929 PstI C_TGCA'G Cuts at: 830 3165 3281 830 Size: 2335 116 3478 Fragments arranged by size: 3478 2335 116 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5929 PvuII CAG'CTG Cuts at: 2396 3028 2396 Size: 632 5297 SalI G'TCGA_C Cuts at: 1936 3271 1936 Size: 1335 4594 SbfI CC_TGCA'GG Cuts at: 3281 3281 Size: 5929 ScaI AGT'ACT Cuts at: 4177 5889 4177 Size: 1712 4217 SexAI A'CCwGG_T Cuts at: 2631 2631 Size: 5929 SfcI C'TryA_G Cuts at: 826 1045 2391 3161 3277 4412 5093 5284 826 Size: 219 1346 770 116 1135 681 191 1471 Fragments arranged by size: 1471 1346 1135 770 681 219 191 116 SfoI GGC'GCC Cuts at: 2372 2372 Size: 5929 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5929 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5929 SmaI CCC'GGG Cuts at: 1872 3256 1872 Size: 1384 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4033 4934 5196 820 Size: 197 926 2090 901 262 1553 Fragments arranged by size: 2090 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3720 493 Size: 3227 2702 SpeI A'CTAG_T Cuts at: 152 2599 152 Size: 2447 3482 SphI G_CATG'C Cuts at: 1132 3287 1132 Size: 2155 3774 SspI AAT'ATT Cuts at: 5 52 3853 5 Size: 47 3801 2081 Fragments arranged by size: 3801 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5929 StyI C'CwwG_G Cuts at: 513 1065 3115 3368 513 Size: 552 2050 253 3074 Fragments arranged by size: 3074 2050 552 253 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3956 3973 4126 4311 4756 325 Size: 1255 2376 17 153 185 445 1498 Fragments arranged by size: 2376 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2353 2508 3037 Size: 274 80 33 51 1443 376 155 529 Cuts at: 3037 4175 5887 96 Size: 1138 1712 138 Fragments arranged by size: 1712 1443 1138 529 376 274 155 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2394 2426 5889 5921 2394 Size: 32 3463 32 2402 Fragments arranged by size: 3463 2402 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5929 XbaI T'CTAG_A Cuts at: 3265 3265 Size: 5929 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5929 XmaI C'CCGG_G Cuts at: 1870 3254 1870 Size: 1384 4545 XmnI GAAnn'nnTTC Cuts at: 4058 4058 Size: 5929 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3737 276 Size: 53 83 186 936 2203 2468 Fragments arranged by size: 2468 2203 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI