(Linear) (Six-base) MAPSORT of: KIEE4387.seq Check: 3900 from: 1 to: 1224 KIEE4387.orf With 182 enzymes: SgfI * March 22, 2012 17:53 .. AccI GT'mk_AC Cuts at: 0 512 1224 Size: 512 712 Acc65I G'GTAC_C Cuts at: 0 728 1224 Size: 728 496 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 795 1070 1224 Size: 795 275 154 Fragments arranged by size: 795 275 154 AleI CACnn'nnGTG Cuts at: 0 1040 1224 Size: 1040 184 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 838 1224 Size: 838 386 AlwNI CAG_nnn'CTG Cuts at: 0 879 1164 1224 Size: 879 285 60 Fragments arranged by size: 879 285 60 ApaI G_GGCC'C Cuts at: 0 1118 1224 Size: 1118 106 ApoI r'AATT_y Cuts at: 0 280 1060 1224 Size: 280 780 164 Fragments arranged by size: 780 280 164 BanI G'GyrC_C Cuts at: 0 298 365 450 728 988 1224 Size: 298 67 85 278 260 236 Fragments arranged by size: 298 278 260 236 85 67 BanII G_rGCy'C Cuts at: 0 1118 1224 Size: 1118 106 BbeI G_GCGC'C Cuts at: 0 369 1224 Size: 369 855 BbsI GAAGACnn'nnnn_ Cuts at: 0 612 1224 Size: 612 612 BbvCI CC'TCA_GC Cuts at: 0 872 1224 Size: 872 352 BclI T'GATC_A Cuts at: 0 35 179 657 1224 Size: 35 144 478 567 Fragments arranged by size: 567 478 144 35 Bme1580I G_kGCm'C Cuts at: 0 303 455 991 1118 1224 Size: 303 152 536 127 106 Fragments arranged by size: 536 303 152 127 106 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 961 1003 1224 Size: 961 42 221 Fragments arranged by size: 961 221 42 Bpu10I CC'TnA_GC Cuts at: 0 872 1224 Size: 872 352 BsaHI Gr'CG_yC Cuts at: 0 366 1224 Size: 366 858 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 389 419 1224 Size: 389 30 805 Fragments arranged by size: 805 389 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 397 1224 Size: 397 827 BseYI C'CCAG_C Cuts at: 0 57 1224 Size: 57 1167 BsmI GAATG_Cn' Cuts at: 0 265 1224 Size: 265 959 BsmBI CGTCTCn'nnnn_ Cuts at: 0 54 1224 Size: 54 1170 Bsp1286I G_dGCh'C Cuts at: 0 303 455 991 1118 1224 Size: 303 152 536 127 106 Fragments arranged by size: 536 303 152 127 106 BspHI T'CATG_A Cuts at: 0 680 1224 Size: 680 544 BsrDI GCAATG_nn' Cuts at: 0 107 1138 1224 Size: 107 1031 86 Fragments arranged by size: 1031 107 86 BssHII G'CGCG_C Cuts at: 0 115 1224 Size: 115 1109 BstYI r'GATC_y Cuts at: 0 158 1160 1224 Size: 158 1002 64 Fragments arranged by size: 1002 158 64 BtgI C'CryG_G Cuts at: 0 788 1224 Size: 788 436 BtsI GCAGTG_nn' Cuts at: 0 262 536 865 1176 1224 Size: 262 274 329 311 48 Fragments arranged by size: 329 311 274 262 48 DraI TTT'AAA Cuts at: 0 1001 1224 Size: 1001 223 EaeI y'GGCC_r Cuts at: 0 68 585 1224 Size: 68 517 639 Fragments arranged by size: 639 517 68 EarI CTCTTCn'nnn_ Cuts at: 0 214 1216 1224 Size: 214 1002 8 Fragments arranged by size: 1002 214 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 454 1224 Size: 454 770 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 795 961 1003 1070 1224 Size: 795 166 42 67 154 Fragments arranged by size: 795 166 154 67 42 EcoO109I rG'GnC_Cy Cuts at: 0 132 1224 Size: 132 1092 EcoRI G'AATT_C Cuts at: 0 1060 1224 Size: 1060 164 EcoRV GAT'ATC Cuts at: 0 1195 1224 Size: 1195 29 HaeII r_GCGC'y Cuts at: 0 369 1224 Size: 369 855 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 143 175 908 940 1184 1216 1224 Size: 143 32 733 32 244 32 8 Fragments arranged by size: 733 244 143 32 32 32 8 KasI G'GCGC_C Cuts at: 0 365 1224 Size: 365 859 KpnI G_GTAC'C Cuts at: 0 732 1224 Size: 732 492 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 617 1224 Size: 617 607 MscI TGG'CCA Cuts at: 0 70 587 1224 Size: 70 517 637 Fragments arranged by size: 637 517 70 MslI CAynn'nnrTG Cuts at: 0 448 1040 1224 Size: 448 592 184 Fragments arranged by size: 592 448 184 MspA1I CmG'CkG Cuts at: 0 30 185 391 1023 1224 Size: 30 155 206 632 201 Fragments arranged by size: 632 206 201 155 30 NarI GG'CG_CC Cuts at: 0 366 1224 Size: 366 858 PasI CC'CwG_GG Cuts at: 0 171 1224 Size: 171 1053 PpuMI rG'GwC_Cy Cuts at: 0 132 1224 Size: 132 1092 PspOMI G'GGCC_C Cuts at: 0 1114 1224 Size: 1114 110 PstI C_TGCA'G Cuts at: 0 1160 1224 Size: 1160 64 PvuII CAG'CTG Cuts at: 0 391 1023 1224 Size: 391 632 201 Fragments arranged by size: 632 391 201 SexAI A'CCwGG_T Cuts at: 0 626 1224 Size: 626 598 SfcI C'TryA_G Cuts at: 0 386 1156 1224 Size: 386 770 68 Fragments arranged by size: 770 386 68 SfoI GGC'GCC Cuts at: 0 367 1224 Size: 367 857 SpeI A'CTAG_T Cuts at: 0 594 1224 Size: 594 630 StyI C'CwwG_G Cuts at: 0 1110 1224 Size: 1110 114 TatI w'GTAC_w Cuts at: 0 348 503 1032 1224 Size: 348 155 529 192 Fragments arranged by size: 529 348 192 155 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 389 421 1224 Size: 389 32 803 Fragments arranged by size: 803 389 32 Enzymes that do cut: AccI Acc65I AcuI AleI AlfI AlwNI ApaI ApoI BanI BanII BbeI BbsI BbvCI BclI Bme1580I BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsmI BsmBI Bsp1286I BspHI BsrDI BssHII BstYI BtgI BtsI DraI EaeI EarI EciI Eco57MI EcoO109I EcoRI EcoRV HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NarI PasI PpuMI PspOMI PstI PvuII SexAI SfcI SfoI SpeI StyI TatI TstI Enzymes that do not cut: AarI AatII AclI AfeI AflII AflIII AgeI AhdI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsgI BsiEI BsiHKAI BsiWI BspEI BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI EcoICRI EcoNI FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI ZraI