################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BanI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsiEI BsiWI BsmAI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI Ksp632I MamI MfeI MluI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 160 GACGTC GACGT^C AccIII 212 TCCGGA T^CCGGA AcyI 160 GACGTC G[GA]^CG[CT]C AocI 856 CCTGAGG CC^T[ACGT]AGG Aor51HI 123 AGCGCT AGC^GCT ApaI 317 GGGCCC GGGCC^C AvaI 57 CCCGGG C^[CT]CG[GA]G AviII 662 TGCGCA TGC^GCA BamHI 962 GGATCC G^GATCC BbsI 382 GTCTTC GAAGAC(2/6) BcgI 806 GCATCGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 875 CTAG C^TAG BpuAI 382 GTCTTC GAAGAC(2/6) BsaHI 160 GACGTC G[GA]^CG[CT]C BsaWI 212 TCCGGA [AT]^CCGG[AT] BseAI 212 TCCGGA T^CCGGA BsmFI 537 GTCCC GGGAC(10/14) BsoBI 57 CCCGGG C^[CT]CG[GA]G BspEI 212 TCCGGA T^CCGGA BsrFI 455 ACCGGC [GA]^CCGG[CT] BssHII 80 GCGCGC G^CGCGC BssSI 292 CTCGTG CACGAG(-5/-1) BstAPI 713 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 856 CCTGAGG CC^T[ACGT]AGG Cfr10I 455 ACCGGC [GA]^CCGG[CT] Eco47III 123 AGCGCT AGC^GCT Eco81I 856 CCTGAGG CC^T[ACGT]AGG EcoNI 321 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 1010 CCAAGG C^C[AT][AT]GG FspI 662 TGCGCA TGC^GCA HgaI 368 GCGTC GACGC(5/10) Hin1I 160 GACGTC G[GA]^CG[CT]C KspI 131 CCGCGG CCGC^GG MaeI 875 CTAG C^TAG MaeII 161 ACGT A^CGT MroI 212 TCCGGA T^CCGGA PleI 240 GAGTC GAGTC(4/5) PstI 1033 CTGCAG CTGCA^G PvuII 900 CAGCTG CAG^CTG SacII 131 CCGCGG CCGC^GG SexAI 257 ACCTGGT A^CC[AT]GGT SfcI 1033 CTGCAG C^T[GA][CT]AG SmaI 57 CCCGGG CCC^GGG StyI 1010 CCAAGG C^C[AT][AT]GG TaqI 102 TCGA T^CGA TfiI 449 GAATC G^A[AT]TC Tsp509I 226 AATT ^AATT TthHB8I 102 TCGA T^CGA XcmI 521 CCAACTTCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 57 CCCGGG C^CCGGG XmaI 57 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 546 GTAC GT^AC 597 GTAC AvaII 95 GGACC G^G[AT]CC 536 GGTCC BalI 296 TGGCCA TGG^CCA 821 TGGCCA BcnI 57 CCCGG CC^[GC]GG 58 CCGGG BsmI 909 GAATGC GAATGC(1/-1) 995 GCATTC BspMI 659 ACCTGC ACCTGC(4/8) 220 GCAGGT BsrI 885 ACTGG ACTGG(1/-1) 594 CCAGT BstF5I 576 CATCC GGATG(2/0) 618 CATCC BstXI 149 CCACCGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 755 CCATGTTTGTGG BstYI 851 AGATCC [GA]^GATC[CT] 962 GGATCC EaeI 296 TGGCCA [CT]^GGCC[GA] 821 TGGCCA FokI 576 CATCC GGATG(9/13) 618 CATCC HinfI 240 GAGTC G^A[ACGT]TC 449 GAATC MaeIII 145 GTCAC ^GT[ACGT]AC 871 GTCAC MboII 302 TCTTC GAAGA(8/7) 383 TCTTC MflI 851 AGATCC [GA]^GATC[CT] 962 GGATCC MluNI 296 TGGCCA TGG^CCA 821 TGGCCA MscI 296 TGGCCA TGG^CCA 821 TGGCCA MseI 407 TTAA T^TAA 800 TTAA MspA1I 131 CCGCGG C[AC]G^C[GT]G 900 CAGCTG NciI 57 CCCGG CC^[GC]GG 58 CCGGG NlaIII 435 CATG CATG^ 756 CATG PpuMI 94 AGGACCC [GA]G^G[AT]CC[CT] 535 GGGTCCC RsaI 546 GTAC GT^AC 597 GTAC Tru9I 407 TTAA T^TAA 800 TTAA Tsp45I 145 GTCAC ^GT[GC]AC 871 GTCAC XhoII 851 AGATCC [GA]^GATC[CT] 962 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AluI AlwI AspHI BanII BbvI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboI MnlI MslI MspI MvaI MvnI MwoI NdeII NlaIV PflMI Sau3AI Sau96I ScrFI SfaNI TseI TspRI Van91I