(Circular) (Six-base) MAPSORT of: pFN21AE9562.seq Check: 9229 from: 1 to: 5791 pFN21AE9562 5791 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2169 3601 278 Size: 53 83 186 936 633 1432 2468 Fragments arranged by size: 2468 1432 936 633 186 83 53 AccI GT'mk_AC Cuts at: 1937 3134 1937 Size: 1197 4594 Acc65I G'GTAC_C Cuts at: 3112 3112 Size: 5791 AclI AA'CG_TT Cuts at: 1563 3918 4291 1563 Size: 2355 373 3063 Fragments arranged by size: 3063 2355 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2672 2750 3854 5238 5506 1632 Size: 1040 78 1104 1384 268 1917 Fragments arranged by size: 1917 1384 1104 1040 268 78 AfeI AGC'GCT Cuts at: 2130 2130 Size: 5791 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5594 AflIII A'CryG_T Cuts at: 1203 4690 5667 1203 Size: 3487 977 1327 Fragments arranged by size: 3487 1327 977 AgeI A'CCGG_T Cuts at: 4622 4622 Size: 5791 AhdI GACnn_n'nnGTC Cuts at: 4520 4520 Size: 5791 AleI CACnn'nnGTG Cuts at: 1166 2159 2510 1166 Size: 993 351 4447 Fragments arranged by size: 4447 993 351 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2693 1184 Size: 1509 4282 AlwNI CAG_nnn'CTG Cuts at: 3534 5106 3534 Size: 1572 4219 ApaI G_GGCC'C Cuts at: 2326 2326 Size: 5791 ApaLI G'TGCA_C Cuts at: 3848 5004 3848 Size: 1156 4635 ApoI r'AATT_y Cuts at: 1493 3100 3326 3478 5587 1493 Size: 1607 226 152 2109 1697 Fragments arranged by size: 2109 1697 1607 226 152 AseI AT'TA_AT Cuts at: 160 1032 4345 160 Size: 872 3313 1606 Fragments arranged by size: 3313 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 AvaI C'yCGr_G Cuts at: 1870 1943 2062 3116 1870 Size: 73 119 1054 4545 Fragments arranged by size: 4545 1054 119 73 BamHI G'GATC_C Cuts at: 2967 3121 3488 2967 Size: 154 367 5270 Fragments arranged by size: 5270 367 154 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3112 4567 618 Size: 325 217 627 1325 1455 1842 Fragments arranged by size: 1842 1455 1325 627 325 217 BanII G_rGCy'C Cuts at: 1391 2326 2800 2934 3047 3110 1391 Size: 935 474 134 113 63 4072 Fragments arranged by size: 4072 935 474 134 113 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2380 928 Size: 971 481 4339 Fragments arranged by size: 4339 971 481 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2800 2834 3982 4016 1903 Size: 34 863 34 1148 34 3678 Fragments arranged by size: 3678 1148 863 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3683 4893 3683 Size: 1210 4581 BclI T'GATC_A Cuts at: 1548 1548 Size: 5791 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4402 136 Size: 107 122 71 631 605 2730 1525 Fragments arranged by size: 2730 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5786 5786 Size: 5791 BlpI GC'TnA_GC Cuts at: 3208 3208 Size: 5791 Bme1580I G_kGCm'C Cuts at: 1790 2326 3852 5008 1790 Size: 536 1526 1156 2573 Fragments arranged by size: 2573 1526 1156 536 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5791 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2593 3522 4480 Size: 416 53 83 399 24 1176 929 958 Cuts at: 4480 442 Size: 1753 Fragments arranged by size: 1753 1176 958 929 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5791 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2035 3066 2035 Size: 1031 4760 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2080 2109 2567 4451 1239 Size: 841 29 458 1884 2579 Fragments arranged by size: 2579 1884 841 458 29 Bpu10I CC'TnA_GC Cuts at: 2201 2369 2201 Size: 168 5623 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3916 4781 5079 3916 Size: 865 298 4628 Fragments arranged by size: 4628 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4454 882 Size: 617 2955 2219 Fragments arranged by size: 2955 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3582 493 Size: 711 2378 2702 Fragments arranged by size: 2702 2378 711 BsaBI GATnn'nnATC Cuts at: 5785 5785 Size: 5791 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2166 3598 3980 4615 Size: 53 83 186 936 633 1432 382 635 Cuts at: 4615 275 Size: 1451 Fragments arranged by size: 1451 1432 936 635 633 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2217 4224 4622 4896 5043 5454 2217 Size: 2007 398 274 147 411 2554 Fragments arranged by size: 2554 2007 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5566 Fragments arranged by size: 5566 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2348 2492 2624 1186 Size: 1162 144 132 4353 Fragments arranged by size: 4353 1162 144 132 BseYI C'CCAG_C Cuts at: 2319 2735 2793 3046 4994 5490 2319 Size: 416 58 253 1948 496 2620 Fragments arranged by size: 2620 1948 496 416 253 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2465 2557 3089 1163 Size: 573 729 92 532 3865 Fragments arranged by size: 3865 729 573 532 92 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3241 4002 5030 1540 Size: 275 186 1240 761 1028 2301 Fragments arranged by size: 2301 1240 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2145 2202 2340 3852 3937 5008 2145 Size: 57 138 1512 85 1071 2928 Fragments arranged by size: 2928 1512 1071 138 85 57 BsmI GAATG_Cn' Cuts at: 2920 3000 3302 3395 2920 Size: 80 302 93 5316 Fragments arranged by size: 5316 302 93 80 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2202 2326 2340 2800 2934 3047 Size: 399 355 57 124 14 460 134 113 Cuts at: 3047 3110 3852 3937 5008 1391 Size: 63 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 460 399 355 134 124 113 85 63 57 14 BspEI T'CCGG_A Cuts at: 2217 5454 2217 Size: 3237 2554 BspHI T'CATG_A Cuts at: 3680 5410 3680 Size: 1730 4061 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2216 2673 3146 844 Size: 1372 457 473 3489 Fragments arranged by size: 3489 1372 473 457 BsrBI CCG'CTC Cuts at: 1394 1823 3678 1394 Size: 429 1855 3507 Fragments arranged by size: 3507 1855 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4286 4460 65 Size: 1183 3038 174 1396 Fragments arranged by size: 3038 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2460 4435 4622 1700 Size: 110 650 1975 187 2869 Fragments arranged by size: 2869 1975 650 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5791 BssHII G'CGCG_C Cuts at: 1925 2085 1925 Size: 160 5631 BssSI C'ACGA_G Cuts at: 2297 3851 4863 2297 Size: 1554 1012 3225 Fragments arranged by size: 3225 1554 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2724 794 Size: 1930 3861 BstBI TT'CG_AA Cuts at: 4595 4595 Size: 5791 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5791 BstXI CCAn_nnnn'nTGG Cuts at: 2161 2767 2161 Size: 606 5185 BstYI r'GATC_y Cuts at: 1972 2856 2967 3121 3488 3873 3890 5342 5428 Size: 884 111 154 367 385 17 1452 86 Cuts at: 5428 5440 5786 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 884 385 367 346 154 111 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2862 2862 Size: 5791 BtgI C'CryG_G Cuts at: 513 1065 2136 2160 2508 2990 513 Size: 552 1071 24 348 482 3314 Fragments arranged by size: 3314 1071 552 482 348 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2389 487 Size: 683 56 1163 3889 Fragments arranged by size: 3889 1163 683 56 BtsI GCAGTG_nn' Cuts at: 2061 2338 2397 2619 2995 3307 4101 4121 2061 Size: 277 59 222 376 312 794 20 3731 Fragments arranged by size: 3731 794 376 312 277 222 59 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5791 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5756 DraI TTT'AAA Cuts at: 3095 3095 Size: 5791 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4798 809 Size: 507 3482 1802 Fragments arranged by size: 3482 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2301 2826 3238 Size: 54 1425 50 275 18 471 525 412 Cuts at: 3238 4127 5756 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 525 471 412 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3238 1537 Size: 275 1426 4090 Fragments arranged by size: 4090 1426 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3721 1353 Size: 2368 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4378 4752 4898 387 Size: 1064 138 2789 374 146 1280 Fragments arranged by size: 2789 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2080 2109 2567 2672 2750 3854 4451 Size: 393 448 29 458 105 78 1104 597 Cuts at: 4451 5238 5506 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1104 787 597 458 448 393 268 105 78 29 EcoNI CCTnn'n_nnAGG Cuts at: 2330 2330 Size: 5791 EcoO109I rG'GnC_Cy Cuts at: 2100 2323 2541 2100 Size: 223 218 5350 Fragments arranged by size: 5350 223 218 EcoRI G'AATT_C Cuts at: 3100 3478 3100 Size: 378 5413 FspI TGC'GCA Cuts at: 2669 4297 2669 Size: 1628 4163 HaeII r_GCGC'y Cuts at: 1678 2132 2355 2467 4938 1678 Size: 454 223 112 2471 2531 Fragments arranged by size: 2531 2471 454 223 112 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4432 4464 4506 4538 1373 Size: 32 135 32 2860 32 42 32 2626 Fragments arranged by size: 2860 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3135 3381 669 Size: 988 281 1197 246 3079 Fragments arranged by size: 3079 1197 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5791 HpaI GTT'AAC Cuts at: 3381 3381 Size: 5791 KpnI G_GTAC'C Cuts at: 3116 3116 Size: 5791 MfeI C'AATT_G Cuts at: 3390 3390 Size: 5791 MluI A'CGCG_T Cuts at: 5667 5667 Size: 5791 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4905 5089 1305 Size: 3600 184 2007 Fragments arranged by size: 3600 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2303 2828 10 Size: 54 1425 343 471 525 2973 Fragments arranged by size: 2973 1425 525 471 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2159 2510 2675 2765 3065 3749 4108 Size: 648 993 351 165 90 300 684 359 Cuts at: 4108 4267 518 Size: 159 2042 Fragments arranged by size: 2042 993 684 648 359 351 300 165 159 90 MspA1I CmG'CkG Cuts at: 1606 2138 2907 3207 3884 5032 5277 1606 Size: 532 769 300 677 1148 245 2120 Fragments arranged by size: 2120 1148 769 677 532 300 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5239 NdeI CA'TA_TG Cuts at: 387 387 Size: 5791 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5791 NotI GC'GGCC_GC Cuts at: 3238 3238 Size: 5791 NspI r_CATG'y Cuts at: 1132 1229 3149 4694 1132 Size: 97 1920 1545 2229 Fragments arranged by size: 2229 1920 1545 97 PasI CC'CwG_GG Cuts at: 1348 2115 2326 1348 Size: 767 211 4813 Fragments arranged by size: 4813 767 211 PciI A'CATG_T Cuts at: 4690 4690 Size: 5791 PflMI CCAn_nnn'nTGG Cuts at: 1108 2440 2793 2845 1108 Size: 1332 353 52 4054 Fragments arranged by size: 4054 1332 353 52 PfoI T'CCnGG_A Cuts at: 2096 2096 Size: 5791 PmeI GTTT'AAAC Cuts at: 3095 3095 Size: 5791 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3860 3892 5373 5405 3860 Size: 32 1481 32 4246 Fragments arranged by size: 4246 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2100 2541 2100 Size: 441 5350 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5791 PsiI TTA'TAA Cuts at: 3361 3361 Size: 5791 PspOMI G'GGCC_C Cuts at: 2322 2322 Size: 5791 PstI C_TGCA'G Cuts at: 830 3042 3143 830 Size: 2212 101 3478 Fragments arranged by size: 3478 2212 101 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5791 PvuII CAG'CTG Cuts at: 2907 2907 Size: 5791 SacII CC_GC'GG Cuts at: 2139 2139 Size: 5791 SalI G'TCGA_C Cuts at: 1936 3133 1936 Size: 1197 4594 SanDI GG'GwC_CC Cuts at: 2541 2541 Size: 5791 SbfI CC_TGCA'GG Cuts at: 3143 3143 Size: 5791 ScaI AGT'ACT Cuts at: 4039 5751 4039 Size: 1712 4079 SexAI A'CCwGG_T Cuts at: 2262 2262 Size: 5791 SfcI C'TryA_G Cuts at: 826 1045 3038 3139 4274 4955 5146 826 Size: 219 1993 101 1135 681 191 1471 Fragments arranged by size: 1993 1471 1135 681 219 191 101 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5791 SmaI CCC'GGG Cuts at: 1872 2064 3118 1872 Size: 192 1054 4545 Fragments arranged by size: 4545 1054 192 SmlI C'TyrA_G Cuts at: 820 1017 1943 3895 4796 5058 820 Size: 197 926 1952 901 262 1553 Fragments arranged by size: 1952 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3582 493 Size: 3089 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5791 SphI G_CATG'C Cuts at: 1132 3149 1132 Size: 2017 3774 SrfI GCCC'GGGC Cuts at: 2064 2064 Size: 5791 SspI AAT'ATT Cuts at: 5 52 3715 5 Size: 47 3663 2081 Fragments arranged by size: 3663 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5791 StyI C'CwwG_G Cuts at: 513 1065 3015 3230 513 Size: 552 1950 215 3074 Fragments arranged by size: 3074 1950 552 215 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2107 2612 2665 3070 3818 3835 3988 Size: 1255 527 505 53 405 748 17 153 Cuts at: 3988 4173 4618 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 748 527 505 445 405 185 153 53 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2601 4037 5749 Size: 274 80 33 51 1443 624 1436 1712 Cuts at: 5749 96 Size: 138 Fragments arranged by size: 1712 1443 1436 624 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5751 5783 5751 Size: 32 5759 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5791 XbaI T'CTAG_A Cuts at: 3127 3127 Size: 5791 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2533 2533 Size: 5791 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5791 XmaI C'CCGG_G Cuts at: 1870 2062 3116 1870 Size: 192 1054 4545 Fragments arranged by size: 4545 1054 192 XmnI GAAnn'nnTTC Cuts at: 3920 3920 Size: 5791 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2167 3599 276 Size: 53 83 186 936 633 1432 2468 Fragments arranged by size: 2468 1432 936 633 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SapI SfiI SfoI SwaI