################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII Acc65I AccIII AflIII AgeI AhdI AocI AscI AseI AsnI Asp718I AspEI AspI BcgI BsaWI BseAI BsiWI BspDI BspEI BspLU11I BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I ClaI DraI DraII DrdI Eam1105I Eco81I EcoO109I EcoRI FseI HindIII HpaI KpnI MluI MroI NruI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI PstI PvuI SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 2185 GTCTAC GT^[AC][GT]AC AclI 1447 AACGTT AA^CGTT AflII 2625 CTTAAG C^TTAAG Aor51HI 2812 AGCGCT AGC^GCT Asp700I 1102 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 2012 GGATCC G^GATCC BbrPI 2649 CACGTG CAC^GTG BbsI 1798 GTCTTC GAAGAC(2/6) BbvCI 2920 GCTGAGG CCTCAGC(-5/-2) BclI 1199 TGATCA T^GATCA BfrI 2625 CTTAAG C^TTAAG BpuAI 1798 GTCTTC GAAGAC(2/6) BsaAI 2649 CACGTG [CT]AC^GT[GA] BsaI 1954 GGTCTC GGTCTC(1/5) BsmBI 3482 CGTCTC CGTCTC(1/5) BspMI 2341 ACCTGC ACCTGC(4/8) BstAPI 1646 GCAAGGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CpoI 176 CGGTCCG CG^G[AT]CCG DraIII 2400 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 2812 AGCGCT AGC^GCT EcoNI 1835 CCTCAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 947 GATATC GAT^ATC FbaI 1199 TGATCA T^GATCA HincII 2587 GTCAAC GT[CT]^[GA]AC HindII 2587 GTCAAC GT[CT]^[GA]AC MfeI 1292 CAATTG C^AATTG MunI 1292 CAATTG C^AATTG NcoI 602 CCATGG C^CATGG NdeI 2100 CATATG CA^TATG NheI 2355 GCTAGC G^CTAGC PflMI 1291 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2649 CACGTG CAC^GTG PmlI 2649 CACGTG CAC^GTG Psp1406I 1447 AACGTT AA^CGTT RsrII 176 CGGTCCG CG^G[AT]CCG SapI 2667 GAAGAGC GCTCTTC(1/4) SspI 557 AATATT AAT^ATT Van91I 1291 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1102 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Alw44I 56 GTGCAC G^TGCAC 649 GTGCAC ApaI 2654 GGGCCC GGGCC^C 2869 GGGCCC ApaLI 56 GTGCAC G^TGCAC 649 GTGCAC AvrII 2211 CCTAGG C^CTAGG 2439 CCTAGG BciVI 2233 GGATAC GTATCC(6/5) 3473 GTATCC BglI 2428 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2940 GCCCGCGCGGC BglII 1522 AGATCT A^GATCT 2036 AGATCT BlnI 2211 CCTAGG C^CTAGG 2439 CCTAGG BlpI 586 GCTCAGC GC^T[ACGT]AGC 2976 GCTGAGC Bpu1102I 586 GCTCAGC GC^T[ACGT]AGC 2976 GCTGAGC BsrBI 411 CCGCTC CCGCTC(-3/-3) 3178 CCGCTC BsrDI 1740 CATTGC GCAATG(2/0) 1905 CATTGC BssHII 3046 GCGCGC G^CGCGC 3281 GCGCGC BstEII 1726 GGTGACC G^GT[ACGT]ACC 3096 GGTCACC BstPI 1726 GGTGACC G^GT[ACGT]ACC 3096 GGTCACC CelII 586 GCTCAGC GC^T[ACGT]AGC 2976 GCTGAGC EcoO65I 1726 GGTGACC G^GT[ACGT]ACC 3096 GGTCACC MseI 1474 TTAA T^TAA 2626 TTAA NotI 2946 GCGGCCGC GC^GGCCGC 3355 GCGGCCGC PvuII 1584 CAGCTG CAG^CTG 1745 CAGCTG SmaI 812 CCCGGG CCC^GGG 3323 CCCGGG SmlI 1836 CTCAAG C^T[CT][GA]AG 2625 CTTAAG SphI 1651 GCATGC GCATG^C 2531 GCATGC Tru9I 1474 TTAA T^TAA 2626 TTAA XmaCI 812 CCCGGG C^CCGGG 3323 CCCGGG XmaI 812 CCCGGG C^CCGGG 3323 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII AviII BalI BanI BanII BbeI BbvI BcnI BfaI BmyI BpmI BsaBI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspHI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EagI EarI EclXI Eco52I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NsiI NspI PleI RcaI RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SspBI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII