(Circular) (Six-base) MAPSORT of: pFN21AE9555.seq Check: 4516 from: 1 to: 8245 pFN21AE9555 8245 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6055 278 Size: 53 83 186 936 4519 2468 Fragments arranged by size: 4519 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4191 5588 1937 Size: 2254 1397 4594 Fragments arranged by size: 4594 2254 1397 Acc65I G'GTAC_C Cuts at: 5566 5566 Size: 8245 AclI AA'CG_TT Cuts at: 1563 3453 6372 6745 1563 Size: 1890 2919 373 3063 Fragments arranged by size: 3063 2919 1890 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2346 2568 2807 3050 3926 5398 6308 7692 Size: 714 222 239 243 876 1472 910 1384 Cuts at: 7692 7960 1632 Size: 268 1917 Fragments arranged by size: 1917 1472 1384 910 876 714 268 243 239 222 AfeI AGC'GCT Cuts at: 4819 4819 Size: 8245 AflII C'TTAA_G Cuts at: 820 1017 4630 820 Size: 197 3613 4435 Fragments arranged by size: 4435 3613 197 AflIII A'CryG_T Cuts at: 1203 7144 8121 1203 Size: 5941 977 1327 Fragments arranged by size: 5941 1327 977 AgeI A'CCGG_T Cuts at: 7076 7076 Size: 8245 AhdI GACnn_n'nnGTC Cuts at: 6974 6974 Size: 8245 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8245 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4475 1184 Size: 3291 4954 AlwNI CAG_nnn'CTG Cuts at: 4693 4705 5260 5988 7560 4693 Size: 12 555 728 1572 5378 Fragments arranged by size: 5378 1572 728 555 12 ApaI G_GGCC'C Cuts at: 4663 4878 4663 Size: 215 8030 ApaLI G'TGCA_C Cuts at: 2061 2654 6302 7458 2061 Size: 593 3648 1156 2848 Fragments arranged by size: 3648 2848 1156 593 ApoI r'AATT_y Cuts at: 1493 2482 2897 3074 3412 5554 5780 5932 8041 Size: 989 415 177 338 2142 226 152 2109 Cuts at: 8041 1493 Size: 1697 Fragments arranged by size: 2142 2109 1697 989 415 338 226 177 152 AseI AT'TA_AT Cuts at: 160 1032 6799 160 Size: 872 5767 1606 Fragments arranged by size: 5767 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8245 AvaI C'yCGr_G Cuts at: 1870 1943 2817 4877 5328 5351 5431 5570 1870 Size: 73 874 2060 451 23 80 139 4545 Fragments arranged by size: 4545 2060 874 451 139 80 73 23 AvrII C'CTAG_G Cuts at: 4216 4444 4216 Size: 228 8017 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2628 2661 3813 3846 3880 3913 2628 Size: 33 1152 33 34 33 6960 Fragments arranged by size: 6960 1152 34 33 33 33 BamHI G'GATC_C Cuts at: 4017 5575 5942 4017 Size: 1558 367 6320 Fragments arranged by size: 6320 1558 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2877 4859 4942 5177 5566 Size: 325 217 627 1090 1982 83 235 389 Cuts at: 5566 7021 618 Size: 1455 1842 Fragments arranged by size: 1982 1842 1455 1090 627 389 325 235 217 83 BanII G_rGCy'C Cuts at: 1391 2122 3237 4616 4663 4878 5000 5075 5133 Size: 731 1115 1379 47 215 122 75 58 Cuts at: 5133 5339 5534 5564 1391 Size: 206 195 30 4072 Fragments arranged by size: 4072 1379 1115 731 215 206 195 122 75 58 47 30 BbeI G_GCGC'C Cuts at: 2881 4863 4946 5181 2881 Size: 1982 83 235 5945 Fragments arranged by size: 5945 1982 235 83 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3796 928 Size: 971 1897 5377 Fragments arranged by size: 5377 1897 971 BbvCI CC'TCA_GC Cuts at: 4926 4926 Size: 8245 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6436 6470 1903 Size: 34 4499 34 3678 Fragments arranged by size: 4499 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4232 5489 6137 7347 4232 Size: 1257 648 1210 5130 Fragments arranged by size: 5130 1257 1210 648 BclI T'GATC_A Cuts at: 1548 3204 1548 Size: 1656 6589 BfrBI ATG'CAT Cuts at: 4110 4165 4540 4110 Size: 55 375 7815 Fragments arranged by size: 7815 375 55 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4439 4951 6856 Size: 107 122 71 631 605 2767 512 1905 Cuts at: 6856 136 Size: 1525 Fragments arranged by size: 2767 1905 1525 631 605 512 122 107 71 BglII A'GATC_T Cuts at: 3527 4041 8240 3527 Size: 514 4199 3532 Fragments arranged by size: 4199 3532 514 BlpI GC'TnA_GC Cuts at: 2592 4982 5662 2592 Size: 2390 680 5175 Fragments arranged by size: 5175 2390 680 Bme1580I G_kGCm'C Cuts at: 1790 2065 2658 4081 4663 4878 6306 7462 1790 Size: 275 593 1423 582 215 1428 1156 2573 Fragments arranged by size: 2573 1428 1423 1156 593 582 275 215 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8245 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2285 3354 3674 Size: 416 53 83 399 24 868 1069 320 Cuts at: 3674 3704 5976 6934 442 Size: 30 2272 958 1753 Fragments arranged by size: 2272 1753 1069 958 868 416 399 320 83 53 30 24 BmtI G_CTAG'C Cuts at: 1056 4364 1056 Size: 3308 4937 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2369 5152 2369 Size: 2783 5462 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2440 2474 3375 5399 6905 1239 Size: 1201 34 901 2024 1506 2579 Fragments arranged by size: 2579 2024 1506 1201 901 34 Bpu10I CC'TnA_GC Cuts at: 4926 4926 Size: 8245 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3826 6370 7235 7533 3826 Size: 2544 865 298 4538 Fragments arranged by size: 4538 2544 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3965 6908 882 Size: 617 2466 2943 2219 Fragments arranged by size: 2943 2466 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4656 6036 493 Size: 711 3452 1380 2702 Fragments arranged by size: 3452 2702 1380 711 BsaBI GATnn'nnATC Cuts at: 2726 2952 3024 8239 2726 Size: 226 72 5215 2732 Fragments arranged by size: 5215 2732 226 72 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2878 4860 4890 4943 Size: 53 83 186 936 1345 1982 30 53 Cuts at: 4943 5172 5178 6052 6434 7069 275 Size: 229 6 874 382 635 1451 Fragments arranged by size: 1982 1451 1345 936 874 635 382 229 186 83 53 53 30 6 BsaWI w'CCGG_w Cuts at: 6678 7076 7350 7497 7908 6678 Size: 398 274 147 411 7015 Fragments arranged by size: 7015 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3611 3641 1178 Size: 30 165 30 2208 30 5782 Fragments arranged by size: 5782 2208 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2092 2392 2395 4606 4943 5053 5396 5524 Size: 906 300 3 2211 337 110 343 128 Cuts at: 5524 1186 Size: 3907 Fragments arranged by size: 3907 2211 906 343 337 300 128 110 3 BseYI C'CCAG_C Cuts at: 2922 3967 4080 4820 5162 5315 7448 7944 2922 Size: 1045 113 740 342 153 2133 496 3223 Fragments arranged by size: 3223 2133 1045 740 496 342 153 113 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3556 4249 5018 1163 Size: 573 1820 693 769 4390 Fragments arranged by size: 4390 1820 769 693 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4749 4955 5346 5364 5695 6456 Size: 275 186 2748 206 391 18 331 761 Cuts at: 6456 7484 1540 Size: 1028 2301 Fragments arranged by size: 2748 2301 1028 761 391 331 275 206 186 18 BsiHKAI G_wGCw'C Cuts at: 2065 2122 2658 4775 4935 5133 5441 5534 6306 Size: 57 536 2117 160 198 308 93 772 Cuts at: 6306 6391 7462 2065 Size: 85 1071 2848 Fragments arranged by size: 2848 2117 1071 772 536 308 198 160 93 85 57 BsmI GAATG_Cn' Cuts at: 2199 2305 4179 5756 5849 2199 Size: 106 1874 1577 93 4595 Fragments arranged by size: 4595 1874 1577 106 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5493 5493 Size: 8245 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2065 2122 2658 3237 4081 4616 4663 Size: 399 275 57 536 579 844 535 47 Cuts at: 4663 4775 4878 4935 5000 5075 5133 5339 5441 Size: 112 103 57 65 75 58 206 102 Cuts at: 5441 5534 5564 6306 6391 7462 1391 Size: 93 30 742 85 1071 2174 Fragments arranged by size: 2174 1071 844 742 579 536 535 399 275 206 112 103 102 93 85 75 65 58 57 57 47 30 BspEI T'CCGG_A Cuts at: 7908 7908 Size: 8245 BspHI T'CATG_A Cuts at: 2161 2568 4065 6134 7864 2161 Size: 407 1497 2069 1730 2542 Fragments arranged by size: 2542 2069 1730 1497 407 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4355 5600 844 Size: 3511 1245 3489 Fragments arranged by size: 3511 3489 1245 BsrBI CCG'CTC Cuts at: 1394 1823 2418 5185 6132 1394 Size: 429 595 2767 947 3507 Fragments arranged by size: 3507 2767 947 595 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3744 3909 6740 6914 65 Size: 1183 2496 165 2831 174 1396 Fragments arranged by size: 2831 2496 1396 1183 174 165 BsrFI r'CCGG_y Cuts at: 1700 1810 4862 4892 5263 5345 6889 7076 1700 Size: 110 3052 30 371 82 1544 187 2869 Fragments arranged by size: 3052 2869 1544 371 187 110 82 30 BsrGI T'GTAC_A Cuts at: 96 3605 4338 4470 96 Size: 3509 733 132 3871 Fragments arranged by size: 3871 3509 733 132 BssHII G'CGCG_C Cuts at: 1925 5051 5286 1925 Size: 3126 235 4884 Fragments arranged by size: 4884 3126 235 BssSI C'ACGA_G Cuts at: 6305 7317 6305 Size: 1012 7233 BstAPI GCAn_nnn'nTGC Cuts at: 794 3657 794 Size: 2863 5382 BstBI TT'CG_AA Cuts at: 7049 7049 Size: 8245 BstEII G'GTnAC_C Cuts at: 1184 3731 5101 1184 Size: 2547 1370 4328 Fragments arranged by size: 4328 2547 1370 BstYI r'GATC_y Cuts at: 1972 2536 3527 4017 4041 4577 5214 5575 5942 Size: 564 991 490 24 536 637 361 367 Cuts at: 5942 6327 6344 7796 7882 7894 8240 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 991 637 564 536 490 385 367 361 346 86 24 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2053 2607 4527 4783 4836 5154 Size: 552 939 49 554 1920 256 53 318 Cuts at: 5154 5295 5340 513 Size: 141 45 3418 Fragments arranged by size: 3418 1920 939 554 552 318 256 141 53 49 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2713 487 Size: 683 56 1487 6019 Fragments arranged by size: 6019 1487 683 56 BtsI GCAGTG_nn' Cuts at: 2920 3544 5101 5761 6555 6575 2920 Size: 624 1557 660 794 20 4590 Fragments arranged by size: 4590 1557 794 660 624 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8245 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3772 3807 529 Size: 35 3208 35 4967 Fragments arranged by size: 4967 3208 35 35 DraI TTT'AAA Cuts at: 5549 5549 Size: 8245 DraIII CAC_nnn'GTG Cuts at: 4410 4410 Size: 8245 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7252 809 Size: 507 5936 1802 Fragments arranged by size: 5936 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2868 3038 3780 Size: 54 1425 50 275 18 1038 170 742 Cuts at: 3780 4482 4746 4917 4952 5001 5115 5265 5343 Size: 702 264 171 35 49 114 150 78 Cuts at: 5343 5361 5692 6581 8210 8 Size: 18 331 889 1629 43 Fragments arranged by size: 1629 1425 1038 889 742 702 331 275 264 171 170 150 114 78 54 50 49 43 35 18 18 EagI C'GGCC_G Cuts at: 1537 1812 4746 4952 5343 5361 5692 1537 Size: 275 2934 206 391 18 331 4090 Fragments arranged by size: 4090 2934 391 331 275 206 18 EarI CTCTTCn'nnn_ Cuts at: 1353 2361 2413 2618 4598 4667 6175 1353 Size: 1008 52 205 1980 69 1508 3423 Fragments arranged by size: 3423 1980 1508 1008 205 69 52 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6832 7206 7352 387 Size: 1064 138 5243 374 146 1280 Fragments arranged by size: 5243 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2120 5131 5532 2120 Size: 3011 401 4833 Fragments arranged by size: 4833 3011 401 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2346 2440 2474 2568 2807 3050 3375 Size: 393 714 94 34 94 239 243 325 Cuts at: 3375 3926 5398 5399 6308 6905 7692 7960 1239 Size: 551 1472 1 909 597 787 268 1524 Fragments arranged by size: 1524 1472 909 787 714 597 551 393 325 268 243 239 94 94 34 1 EcoNI CCTnn'n_nnAGG Cuts at: 3844 3844 Size: 8245 EcoRI G'AATT_C Cuts at: 5554 5932 5554 Size: 378 7867 EcoRV GAT'ATC Cuts at: 2954 2954 Size: 8245 FspI TGC'GCA Cuts at: 4505 4535 4855 6751 4505 Size: 30 320 1896 5999 Fragments arranged by size: 5999 1896 320 30 FspAI rTGC'GCAy Cuts at: 4535 4535 Size: 8245 HaeII r_GCGC'y Cuts at: 1678 2881 4512 4705 4797 4803 4821 4863 4907 Size: 1203 1631 193 92 6 18 42 44 Cuts at: 4907 4946 5181 7392 1678 Size: 39 235 2211 2531 Fragments arranged by size: 2531 2211 1631 1203 235 193 92 44 42 39 18 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2387 2419 3189 3221 3348 Size: 32 135 32 815 32 770 32 127 Cuts at: 3348 3380 4541 4573 5012 5044 6886 6918 6960 Size: 32 1161 32 439 32 1842 32 42 Cuts at: 6960 6992 1373 Size: 32 2626 Fragments arranged by size: 2626 1842 1161 815 770 439 135 127 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4594 5589 5835 669 Size: 988 281 2656 995 246 3079 Fragments arranged by size: 3079 2656 995 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8245 HpaI GTT'AAC Cuts at: 5835 5835 Size: 8245 KasI G'GCGC_C Cuts at: 2877 4859 4942 5177 2877 Size: 1982 83 235 5945 Fragments arranged by size: 5945 1982 235 83 KpnI G_GTAC'C Cuts at: 5570 5570 Size: 8245 MfeI C'AATT_G Cuts at: 3297 5844 3297 Size: 2547 5698 MluI A'CGCG_T Cuts at: 8121 8121 Size: 8245 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3436 3578 7359 7543 1305 Size: 2131 142 3781 184 2007 Fragments arranged by size: 3781 2131 2007 184 142 MscI TGG'CCA Cuts at: 10 64 1489 1832 2870 3782 4484 10 Size: 54 1425 343 1038 912 702 3771 Fragments arranged by size: 3771 1425 1038 912 702 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2849 3248 4406 4457 5231 6203 6562 Size: 648 1683 399 1158 51 774 972 359 Cuts at: 6562 6721 518 Size: 159 2042 Fragments arranged by size: 2042 1683 1158 972 774 648 399 359 159 51 MspA1I CmG'CkG Cuts at: 1606 3591 3752 5156 5297 5342 5661 6338 7486 Size: 1985 161 1404 141 45 319 677 1148 Cuts at: 7486 7731 1606 Size: 245 2120 Fragments arranged by size: 2120 1985 1404 1148 677 319 245 161 141 45 NaeI GCC'GGC Cuts at: 4864 4894 5265 4864 Size: 30 371 7844 Fragments arranged by size: 7844 371 30 NarI GG'CG_CC Cuts at: 2878 4860 4943 5178 2878 Size: 1982 83 235 5945 Fragments arranged by size: 5945 1982 235 83 NcoI C'CATG_G Cuts at: 513 1065 2004 2607 513 Size: 552 939 603 6151 Fragments arranged by size: 6151 939 603 552 NdeI CA'TA_TG Cuts at: 387 4106 387 Size: 3719 4526 NgoMIV G'CCGG_C Cuts at: 4862 4892 5263 4862 Size: 30 371 7844 Fragments arranged by size: 7844 371 30 NheI G'CTAG_C Cuts at: 1052 4360 1052 Size: 3308 4937 NotI GC'GGCC_GC Cuts at: 4952 5361 5692 4952 Size: 409 331 7505 Fragments arranged by size: 7505 409 331 NsiI A_TGCA'T Cuts at: 4112 4167 4542 4112 Size: 55 375 7815 Fragments arranged by size: 7815 375 55 NspI r_CATG'y Cuts at: 1132 1229 2098 3247 3651 3660 4456 4540 4544 Size: 97 869 1149 404 9 796 84 4 Cuts at: 4544 5603 7148 1132 Size: 1059 1545 2229 Fragments arranged by size: 2229 1545 1149 1059 869 796 404 97 84 9 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8245 PciI A'CATG_T Cuts at: 7144 7144 Size: 8245 PflMI CCAn_nnn'nTGG Cuts at: 1108 3302 1108 Size: 2194 6051 PmeI GTTT'AAAC Cuts at: 5549 5549 Size: 8245 PmlI CAC'GTG Cuts at: 4656 4656 Size: 8245 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6314 6346 7827 7859 6314 Size: 32 1481 32 6700 Fragments arranged by size: 6700 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8245 PsiI TTA'TAA Cuts at: 4142 5815 4142 Size: 1673 6572 PspOMI G'GGCC_C Cuts at: 4659 4874 4659 Size: 215 8030 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3116 3148 3116 Size: 32 8213 PstI C_TGCA'G Cuts at: 830 5597 830 Size: 4767 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8245 PvuII CAG'CTG Cuts at: 3591 3752 3591 Size: 161 8084 RsrII CG'GwC_CG Cuts at: 2182 2182 Size: 8245 SacI G_AGCT'C Cuts at: 2122 5133 5534 2122 Size: 3011 401 4833 Fragments arranged by size: 4833 3011 401 SacII CC_GC'GG Cuts at: 5157 5298 5343 5157 Size: 141 45 8059 Fragments arranged by size: 8059 141 45 SalI G'TCGA_C Cuts at: 1936 5587 1936 Size: 3651 4594 SapI GCTCTTCn'nnn_ Cuts at: 4667 4667 Size: 8245 SbfI CC_TGCA'GG Cuts at: 5597 5597 Size: 8245 ScaI AGT'ACT Cuts at: 6493 8205 6493 Size: 1712 6533 SfcI C'TryA_G Cuts at: 826 1045 2463 2749 3798 5593 6728 7409 7600 Size: 219 1418 286 1049 1795 1135 681 191 Cuts at: 7600 826 Size: 1471 Fragments arranged by size: 1795 1471 1418 1135 1049 681 286 219 191 SfoI GGC'GCC Cuts at: 2879 4861 4944 5179 2879 Size: 1982 83 235 5945 Fragments arranged by size: 5945 1982 235 83 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8245 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8245 SmaI CCC'GGG Cuts at: 1872 2819 5330 5572 1872 Size: 947 2511 242 4545 Fragments arranged by size: 4545 2511 947 242 SmlI C'TyrA_G Cuts at: 820 1017 1943 3841 4630 6349 7250 7512 820 Size: 197 926 1898 789 1719 901 262 1553 Fragments arranged by size: 1898 1719 1553 926 901 789 262 197 SnaBI TAC'GTA Cuts at: 493 6036 493 Size: 5543 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8245 SphI G_CATG'C Cuts at: 1132 3660 4540 5603 1132 Size: 2528 880 1063 3774 Fragments arranged by size: 3774 2528 1063 880 SspI AAT'ATT Cuts at: 5 52 2564 6169 5 Size: 47 2512 3605 2081 Fragments arranged by size: 3605 2512 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8245 StyI C'CwwG_G Cuts at: 513 1065 2004 2607 2811 3062 3769 4216 4444 Size: 552 939 603 204 251 707 447 228 Cuts at: 4444 4899 5684 513 Size: 455 785 3074 Fragments arranged by size: 3074 939 785 707 603 552 455 447 251 228 204 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4408 6272 6289 6442 6627 7072 325 Size: 1255 2828 1864 17 153 185 445 1498 Fragments arranged by size: 2828 1864 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3605 3642 3712 Size: 274 80 33 51 1443 1628 37 70 Cuts at: 3712 4338 4470 6491 8203 96 Size: 626 132 2021 1712 138 Fragments arranged by size: 2021 1712 1628 1443 626 274 138 132 80 70 51 37 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8205 8237 8205 Size: 32 8213 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8245 XbaI T'CTAG_A Cuts at: 5581 5581 Size: 8245 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2272 2818 2878 3220 3751 4525 5374 2272 Size: 546 60 342 531 774 849 5143 Fragments arranged by size: 5143 849 774 546 531 342 60 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8245 XmaI C'CCGG_G Cuts at: 1870 2817 5328 5570 1870 Size: 947 2511 242 4545 Fragments arranged by size: 4545 2511 947 242 XmnI GAAnn'nnTTC Cuts at: 3111 6374 3111 Size: 3263 4982 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6053 276 Size: 53 83 186 936 4519 2468 Fragments arranged by size: 4519 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BsiWI BstXI BstZ17I Bsu36I EcoO109I FalI FseI NruI PacI PfoI PpuMI PspXI SanDI SexAI SfiI SrfI SwaI