################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KspI MaeII MfeI MluI MluNI MroI MscI MseI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 103 GGTACC G^GTACC AfaI 104 GTAC GT^AC AluI 4 AGCT AG^CT AlwI 412 GATCC GGATC(4/5) AocI 415 CCTGAGG CC^T[ACGT]AGG Asp700I 569 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 103 GGTACC G^GTACC AvaII 204 GGACC G^G[AT]CC BanI 103 GGTACC G^G[CT][GA]CC BanII 43 GGGCTC G[GA]GC[CT]^C BbsI 298 GAAGAC GAAGAC(2/6) BciVI 660 GGATAC GTATCC(6/5) BclI 705 TGATCA T^GATCA BglI 24 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 43 GGGCTC G[AGT]GC[ACT]^C BpmI 553 CTGGAG CTGGAG(16/14) BpuAI 298 GAAGAC GAAGAC(2/6) BsaBI 700 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC Bsp1286I 43 GGGCTC G[AGT]GC[ACT]^C BstYI 411 AGATCC [GA]^GATC[CT] Bsu36I 415 CCTGAGG CC^T[ACGT]AGG Cac8I 33 GCCAGC GC[ACGT]^[ACGT]GC DraII 89 AGGGCCT [GA]G^G[ACGT]CC[CT] EarI 292 GAAGAG CTCTTC(1/4) Eco81I 415 CCTGAGG CC^T[ACGT]AGG EcoO109I 89 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoT22I 191 ATGCAT ATGCA^T FauI 21 CCCGC CCCGC(4/6) FbaI 705 TGATCA T^GATCA HphI 243 GGTGA GGTGA(8/7) KpnI 103 GGTACC GGTAC^C Ksp632I 292 GAAGAG CTCTTC(1/4) MaeIII 212 GTGAC ^GT[ACGT]AC MamI 700 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 411 AGATCC [GA]^GATC[CT] MspA1I 35 CAGCGG C[AC]G^C[GT]G NsiI 191 ATGCAT ATGCA^T NspI 189 ACATGC [GA]CATG^[CT] RsaI 104 GTAC GT^AC StuI 398 AGGCCT AGG^CCT Tsp45I 212 GTGAC ^GT[GC]AC XhoII 411 AGATCC [GA]^GATC[CT] XmnI 569 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AcsI 253 AAATTT [GA]^AATT[CT] 331 GAATTT ApoI 253 AAATTT [GA]^AATT[CT] 331 GAATTT BfaI 220 CTAG C^TAG 347 CTAG BsiYI 22 CCGCCGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 34 CCAGCGGAGGG BslI 22 CCGCCGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 34 CCAGCGGAGGG BsmFI 203 GGGAC GGGAC(10/14) 485 GGGAC BsrDI 370 GCAATG GCAATG(2/0) 382 GCAATG DpnII 412 GATC ^GATC 706 GATC MaeI 220 CTAG C^TAG 347 CTAG MboI 412 GATC ^GATC 706 GATC MslI 229 CATTTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 378 CACTGCAATG NdeII 412 GATC ^GATC 706 GATC NlaIII 190 CATG CATG^ 469 CATG Sau3AI 412 GATC ^GATC 706 GATC SfaNI 694 GATGC GCATC(5/9) 405 GCATC Tsp509I 254 AATT ^AATT 332 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AciI AlwNI BbvI BsaJI BsmI BsrI BssKI BstF5I BstNI Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HinfI ItaI MboII MnlI MvaI MwoI NlaIV Sau96I ScrFI TfiI TseI TspRI