(Circular) (Six-base) MAPSORT of: pFN21AB7576.seq Check: 664 from: 1 to: 5557 pFN21AB7576 5557 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3367 278 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2900 1937 Size: 963 4594 Acc65I G'GTAC_C Cuts at: 2878 2878 Size: 5557 AclI AA'CG_TT Cuts at: 1563 3684 4057 1563 Size: 2121 373 3063 Fragments arranged by size: 3063 2121 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2861 3620 5004 5272 1632 Size: 1229 759 1384 268 1917 Fragments arranged by size: 1917 1384 1229 759 268 AflII C'TTAA_G Cuts at: 820 1017 2756 820 Size: 197 1739 3621 Fragments arranged by size: 3621 1739 197 AflIII A'CryG_T Cuts at: 1203 2360 4456 5433 1203 Size: 1157 2096 977 1327 Fragments arranged by size: 2096 1327 1157 977 AgeI A'CCGG_T Cuts at: 4388 4388 Size: 5557 AhdI GACnn_n'nnGTC Cuts at: 4286 4286 Size: 5557 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5557 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5557 AlwNI CAG_nnn'CTG Cuts at: 3300 4872 3300 Size: 1572 3985 ApaI G_GGCC'C Cuts at: 2793 2793 Size: 5557 ApaLI G'TGCA_C Cuts at: 3614 4770 3614 Size: 1156 4401 ApoI r'AATT_y Cuts at: 1493 2866 3092 3244 5353 1493 Size: 1373 226 152 2109 1697 Fragments arranged by size: 2109 1697 1373 226 152 AseI AT'TA_AT Cuts at: 160 1032 4111 160 Size: 872 3079 1606 Fragments arranged by size: 3079 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 AvaI C'yCGr_G Cuts at: 1870 1943 2786 2882 1870 Size: 73 843 96 4545 Fragments arranged by size: 4545 843 96 73 BamHI G'GATC_C Cuts at: 2887 3254 2887 Size: 367 5190 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2078 2183 2489 2878 4333 Size: 325 217 627 291 105 306 389 1455 Cuts at: 4333 618 Size: 1842 Fragments arranged by size: 1842 1455 627 389 325 306 291 217 105 BanII G_rGCy'C Cuts at: 1391 2624 2651 2793 2876 1391 Size: 1233 27 142 83 4072 Fragments arranged by size: 4072 1233 142 83 27 BbeI G_GCGC'C Cuts at: 2082 2187 2493 2082 Size: 105 306 5146 Fragments arranged by size: 5146 306 105 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2849 928 Size: 971 950 3636 Fragments arranged by size: 3636 971 950 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3748 3782 1903 Size: 34 1811 34 3678 Fragments arranged by size: 3678 1811 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3449 4659 3449 Size: 1210 4347 BclI T'GATC_A Cuts at: 1548 1548 Size: 5557 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2054 2094 2708 Size: 107 122 71 631 605 382 40 614 Cuts at: 2708 4168 136 Size: 1460 1525 Fragments arranged by size: 1525 1460 631 614 605 382 122 107 71 40 BglII A'GATC_T Cuts at: 5552 5552 Size: 5557 BlpI GC'TnA_GC Cuts at: 2974 2974 Size: 5557 Bme1580I G_kGCm'C Cuts at: 1790 2226 2793 3618 4774 1790 Size: 436 567 825 1156 2573 Fragments arranged by size: 2573 1156 825 567 436 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5557 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2654 3288 4246 Size: 416 53 83 399 24 1237 634 958 Cuts at: 4246 442 Size: 1753 Fragments arranged by size: 1753 1237 958 634 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5557 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2339 4217 1239 Size: 1100 1878 2579 Fragments arranged by size: 2579 1878 1100 Bpu10I CC'TnA_GC Cuts at: 2618 2618 Size: 5557 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2294 2363 2669 3682 4547 4845 2294 Size: 69 306 1013 865 298 3006 Fragments arranged by size: 3006 1013 865 306 298 69 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2330 4220 882 Size: 617 831 1890 2219 Fragments arranged by size: 2219 1890 831 617 BsaAI yAC'GTr Cuts at: 493 1204 2547 3348 493 Size: 711 1343 801 2702 Fragments arranged by size: 2702 1343 801 711 BsaBI GATnn'nnATC Cuts at: 5551 5551 Size: 5557 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2057 2079 2184 2490 Size: 53 83 186 936 524 22 105 306 Cuts at: 2490 3364 3746 4381 275 Size: 874 382 635 1451 Fragments arranged by size: 1451 936 874 635 524 382 306 186 105 83 53 22 BsaWI w'CCGG_w Cuts at: 2820 3990 4388 4662 4809 5220 2820 Size: 1170 398 274 147 411 3157 Fragments arranged by size: 3157 1170 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2531 2561 1178 Size: 30 165 30 1128 30 4174 Fragments arranged by size: 4174 1128 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2155 2381 2506 1186 Size: 969 226 125 4237 Fragments arranged by size: 4237 969 226 125 BseYI C'CCAG_C Cuts at: 2624 4760 5256 2624 Size: 2136 496 2925 Fragments arranged by size: 2925 2136 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2240 2426 2569 1163 Size: 573 504 186 143 4151 Fragments arranged by size: 4151 573 504 186 143 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2748 3007 3768 4796 1540 Size: 275 186 747 259 761 1028 2301 Fragments arranged by size: 2301 1028 761 747 275 259 186 BsiHKAI G_wGCw'C Cuts at: 3618 3703 4774 3618 Size: 85 1071 4401 Fragments arranged by size: 4401 1071 85 BsiWI C'GTAC_G Cuts at: 2028 2028 Size: 5557 BsmI GAATG_Cn' Cuts at: 3068 3161 3068 Size: 93 5464 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2624 2651 2793 2876 3618 3703 Size: 399 436 398 27 142 83 742 85 Cuts at: 3703 4774 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 436 399 398 142 85 83 27 BspEI T'CCGG_A Cuts at: 5220 5220 Size: 5557 BspHI T'CATG_A Cuts at: 3446 5176 3446 Size: 1730 3827 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2398 2912 844 Size: 1554 514 3489 Fragments arranged by size: 3489 1554 514 BsrBI CCG'CTC Cuts at: 1394 1823 2683 2744 3444 1394 Size: 429 860 61 700 3507 Fragments arranged by size: 3507 860 700 429 61 BsrDI GCAATG_nn' Cuts at: 65 1248 4052 4226 65 Size: 1183 2804 174 1396 Fragments arranged by size: 2804 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2702 2770 4201 4388 1700 Size: 110 892 68 1431 187 2869 Fragments arranged by size: 2869 1431 892 187 110 68 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5557 BssHII G'CGCG_C Cuts at: 1925 2301 2452 2507 1925 Size: 376 151 55 4975 Fragments arranged by size: 4975 376 151 55 BssSI C'ACGA_G Cuts at: 2035 2258 3617 4629 2035 Size: 223 1359 1012 2963 Fragments arranged by size: 2963 1359 1012 223 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5557 BstBI TT'CG_AA Cuts at: 4361 4361 Size: 5557 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5557 BstYI r'GATC_y Cuts at: 1972 2887 3254 3639 3656 5108 5194 5206 5552 Size: 915 367 385 17 1452 86 12 346 Cuts at: 5552 1972 Size: 1977 Fragments arranged by size: 1977 1452 915 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2049 2133 513 Size: 552 939 45 84 3937 Fragments arranged by size: 3937 939 552 84 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4818 Fragments arranged by size: 4818 683 56 BtsI GCAGTG_nn' Cuts at: 2226 3073 3867 3887 2226 Size: 847 794 20 3896 Fragments arranged by size: 3896 847 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5557 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5522 DraI TTT'AAA Cuts at: 2861 2861 Size: 5557 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4564 809 Size: 507 3248 1802 Fragments arranged by size: 3248 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2457 2604 Size: 54 1425 50 275 18 216 411 147 Cuts at: 2604 2745 3004 3893 5522 8 Size: 141 259 889 1629 43 Fragments arranged by size: 1629 1425 889 411 275 259 216 147 141 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2745 3004 1537 Size: 275 933 259 4090 Fragments arranged by size: 4090 933 275 259 EarI CTCTTCn'nnn_ Cuts at: 1353 2858 3487 1353 Size: 1505 629 3423 Fragments arranged by size: 3423 1505 629 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2050 2789 4144 4518 4664 387 Size: 1064 138 461 739 1355 374 146 1280 Fragments arranged by size: 1355 1280 1064 739 461 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2339 2861 3620 4217 5004 5272 1239 Size: 393 707 522 759 597 787 268 1524 Fragments arranged by size: 1524 787 759 707 597 522 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2393 2393 Size: 5557 EcoO109I rG'GnC_Cy Cuts at: 2660 2790 2660 Size: 130 5427 EcoRI G'AATT_C Cuts at: 2866 3244 2866 Size: 378 5179 FspI TGC'GCA Cuts at: 2176 4063 2176 Size: 1887 3670 HaeII r_GCGC'y Cuts at: 1678 2082 2187 2378 2466 2493 4704 1678 Size: 404 105 191 88 27 2211 2531 Fragments arranged by size: 2531 2211 404 191 105 88 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4198 4230 4272 4304 1373 Size: 32 135 32 2626 32 42 32 2626 Fragments arranged by size: 2626 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2901 3147 669 Size: 988 281 963 246 3079 Fragments arranged by size: 3079 988 963 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5557 HpaI GTT'AAC Cuts at: 3147 3147 Size: 5557 KasI G'GCGC_C Cuts at: 2078 2183 2489 2078 Size: 105 306 5146 Fragments arranged by size: 5146 306 105 KpnI G_GTAC'C Cuts at: 2882 2882 Size: 5557 MfeI C'AATT_G Cuts at: 3156 3156 Size: 5557 MluI A'CGCG_T Cuts at: 2360 5433 2360 Size: 3073 2484 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2193 4671 4855 1305 Size: 888 2478 184 2007 Fragments arranged by size: 2478 2007 888 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2606 10 Size: 54 1425 343 774 2961 Fragments arranged by size: 2961 1425 774 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3515 3874 4033 518 Size: 648 2349 359 159 2042 Fragments arranged by size: 2349 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2135 2275 2632 2973 3650 4798 5043 1606 Size: 529 140 357 341 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 529 357 341 245 140 NaeI GCC'GGC Cuts at: 2772 2772 Size: 5557 NarI GG'CG_CC Cuts at: 2079 2184 2490 2079 Size: 105 306 5146 Fragments arranged by size: 5146 306 105 NcoI C'CATG_G Cuts at: 513 1065 2004 2049 513 Size: 552 939 45 4021 Fragments arranged by size: 4021 939 552 45 NdeI CA'TA_TG Cuts at: 387 387 Size: 5557 NgoMIV G'CCGG_C Cuts at: 2770 2770 Size: 5557 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5557 NotI GC'GGCC_GC Cuts at: 3004 3004 Size: 5557 NspI r_CATG'y Cuts at: 1132 1229 2915 4460 1132 Size: 97 1686 1545 2229 Fragments arranged by size: 2229 1686 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5557 PciI A'CATG_T Cuts at: 4456 4456 Size: 5557 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5557 PmeI GTTT'AAAC Cuts at: 2861 2861 Size: 5557 PmlI CAC'GTG Cuts at: 2547 2547 Size: 5557 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3626 3658 5139 5171 3626 Size: 32 1481 32 4012 Fragments arranged by size: 4012 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2660 2660 Size: 5557 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5557 PsiI TTA'TAA Cuts at: 3127 3127 Size: 5557 PspOMI G'GGCC_C Cuts at: 2789 2789 Size: 5557 PstI C_TGCA'G Cuts at: 830 2087 2909 830 Size: 1257 822 3478 Fragments arranged by size: 3478 1257 822 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5557 SacII CC_GC'GG Cuts at: 2136 2136 Size: 5557 SalI G'TCGA_C Cuts at: 1936 2899 1936 Size: 963 4594 SbfI CC_TGCA'GG Cuts at: 2909 2909 Size: 5557 ScaI AGT'ACT Cuts at: 3805 5517 3805 Size: 1712 3845 SfcI C'TryA_G Cuts at: 826 1045 2083 2905 4040 4721 4912 826 Size: 219 1038 822 1135 681 191 1471 Fragments arranged by size: 1471 1135 1038 822 681 219 191 SfoI GGC'GCC Cuts at: 2080 2185 2491 2080 Size: 105 306 5146 Fragments arranged by size: 5146 306 105 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5557 SmaI CCC'GGG Cuts at: 1872 2788 2884 1872 Size: 916 96 4545 Fragments arranged by size: 4545 916 96 SmlI C'TyrA_G Cuts at: 820 1017 1943 2309 2378 2684 2756 3661 4562 Size: 197 926 366 69 306 72 905 901 Cuts at: 4562 4824 820 Size: 262 1553 Fragments arranged by size: 1553 926 905 901 366 306 262 197 72 69 SnaBI TAC'GTA Cuts at: 493 3348 493 Size: 2855 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5557 SphI G_CATG'C Cuts at: 1132 2915 1132 Size: 1783 3774 SspI AAT'ATT Cuts at: 5 52 3481 5 Size: 47 3429 2081 Fragments arranged by size: 3429 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5557 StyI C'CwwG_G Cuts at: 513 1065 2004 2049 2466 2535 2996 513 Size: 552 939 45 417 69 461 3074 Fragments arranged by size: 3074 939 552 461 417 69 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3584 3601 3754 3939 4384 325 Size: 1255 2004 17 153 185 445 1498 Fragments arranged by size: 2004 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3803 5515 96 Size: 274 80 33 51 1443 1826 1712 138 Fragments arranged by size: 1826 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2531 2563 5517 5549 2531 Size: 32 2954 32 2539 Fragments arranged by size: 2954 2539 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5557 XbaI T'CTAG_A Cuts at: 2893 2893 Size: 5557 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5557 XmaI C'CCGG_G Cuts at: 1870 2786 2882 1870 Size: 916 96 4545 Fragments arranged by size: 4545 916 96 XmnI GAAnn'nnTTC Cuts at: 3686 3686 Size: 5557 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3365 276 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI