Restriction map for pFN21AB7576
 (Linear) (Six-base) MAPSORT of: KIBB7576.seq  Check: 5684  from: 1  to: 852



KIBB7576.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:43  ..

AflII C'TTAA_G

Cuts at:      0     751     852 
   Size:        751     101
 
AflIII A'CryG_T

Cuts at:      0     355     852 
   Size:        355     497
 
ApaI G_GGCC'C

Cuts at:      0     788     852 
   Size:        788      64
 
AvaI C'yCGr_G

Cuts at:      0     781     852 
   Size:        781      71
 
BanI G'GyrC_C

Cuts at:      0      73     178     484     852 
   Size:         73     105     306     368

  Fragments arranged by size: 

                 368     306     105      73
 
BanII G_rGCy'C

Cuts at:      0     619     646     788     852 
   Size:        619      27     142      64

  Fragments arranged by size: 

                 619     142      64      27
 
BbeI G_GCGC'C

Cuts at:      0      77     182     488     852 
   Size:         77     105     306     364

  Fragments arranged by size: 

                 364     306     105      77
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     844     852 
   Size:        844       8
 
BglI GCCn_nnn'nGGC

Cuts at:      0      49      89     703     852 
   Size:         49      40     614     149

  Fragments arranged by size: 

                 614     149      49      40
 
Bme1580I G_kGCm'C

Cuts at:      0     221     788     852 
   Size:        221     567      64

  Fragments arranged by size: 

                 567     221      64
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     649     852 
   Size:        649     203
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     852 
   Size:        334     518
 
Bpu10I CC'TnA_GC

Cuts at:      0     613     852 
   Size:        613     239
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     358     664     852 
   Size:        289      69     306     188

  Fragments arranged by size: 

                 306     289     188      69
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     325     852 
   Size:        325     527
 
BsaAI yAC'GTr

Cuts at:      0     542     852 
   Size:        542     310
 
BsaHI Gr'CG_yC

Cuts at:      0      52      74     179     485     852 
   Size:         52      22     105     306     367

  Fragments arranged by size: 

                 367     306     105      52      22
 
BsaWI w'CCGG_w

Cuts at:      0     815     852 
   Size:        815      37
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     526     556     852 
   Size:        526      30     296

  Fragments arranged by size: 

                 526     296      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     150     376     501     852 
   Size:        150     226     125     351

  Fragments arranged by size: 

                 351     226     150     125
 
BseYI C'CCAG_C

Cuts at:      0     619     852 
   Size:        619     233
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     421     564     852 
   Size:        235     186     143     288

  Fragments arranged by size: 

                 288     235     186     143
 
BsiEI CG_ry'CG

Cuts at:      0     743     852 
   Size:        743     109
 
BsiWI C'GTAC_G

Cuts at:      0      23     852 
   Size:         23     829
 
Bsp1286I G_dGCh'C

Cuts at:      0     221     619     646     788     852 
   Size:        221     398      27     142      64

  Fragments arranged by size: 

                 398     221     142      64      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     393     852 
   Size:        393     459
 
BsrBI CCG'CTC

Cuts at:      0     678     739     852 
   Size:        678      61     113

  Fragments arranged by size: 

                 678     113      61
 
BsrFI r'CCGG_y

Cuts at:      0     697     765     852 
   Size:        697      68      87

  Fragments arranged by size: 

                 697      87      68
 
BssHII G'CGCG_C

Cuts at:      0     296     447     502     852 
   Size:        296     151      55     350

  Fragments arranged by size: 

                 350     296     151      55
 
BssSI C'ACGA_G

Cuts at:      0      30     253     852 
   Size:         30     223     599

  Fragments arranged by size: 

                 599     223      30
 
BtgI C'CryG_G

Cuts at:      0      44     128     852 
   Size:         44      84     724

  Fragments arranged by size: 

                 724      84      44
 
BtsI GCAGTG_nn'

Cuts at:      0     221     852 
   Size:        221     631
 
EaeI y'GGCC_r

Cuts at:      0      41     452     599     740     852 
   Size:         41     411     147     141     112

  Fragments arranged by size: 

                 411     147     141     112      41
 
EagI C'GGCC_G

Cuts at:      0     740     852 
   Size:        740     112
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      45     784     852 
   Size:         45     739      68

  Fragments arranged by size: 

                 739      68      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     852 
   Size:        334     518
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     388     852 
   Size:        388     464
 
EcoO109I rG'GnC_Cy

Cuts at:      0     655     785     852 
   Size:        655     130      67

  Fragments arranged by size: 

                 655     130      67
 
FspI TGC'GCA

Cuts at:      0     171     852 
   Size:        171     681
 
HaeII r_GCGC'y

Cuts at:      0      77     182     373     461     488     852 
   Size:         77     105     191      88      27     364

  Fragments arranged by size: 

                 364     191     105      88      77      27
 
KasI G'GCGC_C

Cuts at:      0      73     178     484     852 
   Size:         73     105     306     368

  Fragments arranged by size: 

                 368     306     105      73
 
MluI A'CGCG_T

Cuts at:      0     355     852 
   Size:        355     497
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     188     852 
   Size:        188     664
 
MscI TGG'CCA

Cuts at:      0     601     852 
   Size:        601     251
 
MspA1I CmG'CkG

Cuts at:      0     130     270     627     852 
   Size:        130     140     357     225

  Fragments arranged by size: 

                 357     225     140     130
 
NaeI GCC'GGC

Cuts at:      0     767     852 
   Size:        767      85
 
NarI GG'CG_CC

Cuts at:      0      74     179     485     852 
   Size:         74     105     306     367

  Fragments arranged by size: 

                 367     306     105      74
 
NcoI C'CATG_G

Cuts at:      0      44     852 
   Size:         44     808
 
NgoMIV G'CCGG_C

Cuts at:      0     765     852 
   Size:        765      87
 
PmlI CAC'GTG

Cuts at:      0     542     852 
   Size:        542     310
 
PpuMI rG'GwC_Cy

Cuts at:      0     655     852 
   Size:        655     197
 
PspOMI G'GGCC_C

Cuts at:      0     784     852 
   Size:        784      68
 
PstI C_TGCA'G

Cuts at:      0      82     852 
   Size:         82     770
 
SacII CC_GC'GG

Cuts at:      0     131     852 
   Size:        131     721
 
SfcI C'TryA_G

Cuts at:      0      78     852 
   Size:         78     774
 
SfoI GGC'GCC

Cuts at:      0      75     180     486     852 
   Size:         75     105     306     366

  Fragments arranged by size: 

                 366     306     105      75
 
SmaI CCC'GGG

Cuts at:      0     783     852 
   Size:        783      69
 
SmlI C'TyrA_G

Cuts at:      0     304     373     679     751     852 
   Size:        304      69     306      72     101

  Fragments arranged by size: 

                 306     304     101      72      69
 
StyI C'CwwG_G

Cuts at:      0      44     461     530     852 
   Size:         44     417      69     322

  Fragments arranged by size: 

                 417     322      69      44
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     526     558     852 
   Size:        526      32     294

  Fragments arranged by size: 

                 526     294      32
 
XmaI C'CCGG_G

Cuts at:      0     781     852 
   Size:        781      71
 

 Enzymes that do cut:

    AflII   AflIII     ApaI     AvaI     BanI    BanII     BbeI     BbsI
     BglI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI
 Bsp1286I    BspMI    BsrBI    BsrFI   BssHII    BssSI     BtgI     BtsI
     EaeI     EagI     EciI  Eco57MI    EcoNI EcoO109I     FspI    HaeII
     KasI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     PmlI    PpuMI   PspOMI     PstI    SacII     SfcI     SfoI
     SmaI     SmlI     StyI     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI    BsaBI
  BsiHKAI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EarI  EcoICRI    EcoRI    EcoRV
     FalI     FseI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MslI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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