################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbeI BbrPI BbsI BcgI BclI BcnI BfrI BglI BglII BlnI BpmI BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstBI BstEII BstF5I BstPI BstUI BstYI BstZ17I Bsu36I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI HaeII HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MroI MspA1I MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp509I Tth111I Van91I XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AciI 489 CCGC CCGC(-3/-1) AlwI 717 GGATC GGATC(4/5) AspHI 194 GAGCTC G[AT]GC[AT]^C AviII 473 TGCGCA TGC^GCA BbvCI 175 CCTCAGC CCTCAGC(-5/-2) BciVI 276 GTATCC GTATCC(6/5) BlpI 70 GCTCAGC GC^T[ACGT]AGC Bpu1102I 70 GCTCAGC GC^T[ACGT]AGC BsaAI 188 TACGTA [CT]AC^GT[GA] BsiHKAI 194 GAGCTC G[AT]GC[AT]^C BsmFI 724 GGGAC GGGAC(10/14) BspMI 180 GCAGGT ACCTGC(4/8) BsrFI 355 ACCGGC [GA]^CCGG[CT] BssSI 206 CACGAG CACGAG(-5/-1) BstXI 105 CCAGAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 70 GCTCAGC GC^T[ACGT]AGC CfoI 474 GCGC GCG^C Cfr10I 355 ACCGGC [GA]^CCGG[CT] DpnII 718 GATC ^GATC DraII 724 GGGACCT [GA]G^G[ACGT]CC[CT] DraIII 463 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 724 GGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 713 CCTTGG C^C[AT][AT]GG FspI 473 TGCGCA TGC^GCA HapII 356 CCGG C^CGG HhaI 474 GCGC GCG^C HinP1I 474 GCGC G^CGC HincII 289 GTTGAC GT[CT]^[GA]AC HindII 289 GTTGAC GT[CT]^[GA]AC HinfI 434 GAATC G^A[ACGT]TC HpaII 356 CCGG C^CGG HphI 247 TCACC GGTGA(8/7) MaeIII 246 GTCAC ^GT[ACGT]AC MboI 718 GATC ^GATC MspI 356 CCGG C^CGG NdeI 689 CATATG CA^TATG NdeII 718 GATC ^GATC PaeR7I 842 CTCGAG C^TCGAG PpuMI 724 GGGACCT [GA]G^G[AT]CC[CT] SacI 194 GAGCTC GAGCT^C Sau3AI 718 GATC ^GATC SmlI 842 CTCGAG C^T[CT][GA]AG SnaBI 188 TACGTA TAC^GTA StyI 713 CCTTGG C^C[AT][AT]GG TfiI 434 GAATC G^A[AT]TC Tsp45I 246 GTCAC ^GT[GC]AC XbaI 793 TCTAGA T^CTAGA XhoI 842 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 187 GTAC GT^AC 684 GTAC AvaI 23 CCCGAG C^[CT]CG[GA]G 842 CTCGAG AvaII 353 GGACC G^G[AT]CC 725 GGACC BalI 114 TGGCCA TGG^CCA 507 TGGCCA BanII 20 GAGCCC G[GA]GC[CT]^C 194 GAGCTC BsoBI 23 CCCGAG C^[CT]CG[GA]G 842 CTCGAG BsrDI 66 CATTGC GCAATG(2/0) 545 GCAATG BstAPI 462 GCACCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 768 GCATCGAGTGC Cac8I 18 GCGAGC GC[ACGT]^[ACGT]GC 75 GCCAGC Cfr13I 353 GGACC G^G[ACGT]CC 725 GGACC EaeI 114 TGGCCA [CT]^GGCC[GA] 507 TGGCCA EarI 496 GAAGAG CTCTTC(1/4) 670 GAAGAG Ksp632I 496 GAAGAG CTCTTC(1/4) 670 GAAGAG MaeII 189 ACGT A^CGT 293 ACGT MluNI 114 TGGCCA TGG^CCA 507 TGGCCA MscI 114 TGGCCA TGG^CCA 507 TGGCCA MseI 53 TTAA T^TAA 733 TTAA MslI 571 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 687 CACATATGTG RsaI 187 GTAC GT^AC 684 GTAC Sau96I 353 GGACC G^G[ACGT]CC 725 GGACC TaqI 771 TCGA T^CGA 843 TCGA Tru9I 53 TTAA T^TAA 733 TTAA TthHB8I 771 TCGA T^CGA 843 TCGA XcmI 102 CCACCAGAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 448 CCAAATCCTCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AluI BanI BbvI BfaI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstNI DdeI EcoRII Fnu4HI HaeIII ItaI MaeI MboII MnlI MvaI MwoI NlaIII NlaIV ScrFI SfaNI TseI TspRI