(Circular) (Six-base) MAPSORT of: pFN21AE2410.seq Check: 4067 from: 1 to: 5563 pFN21AE2410 5563 bp With 182 enzymes: SgfI * May 24, 2012 15:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3373 278 Size: 53 83 186 936 1837 2468 Fragments arranged by size: 2468 1837 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2906 1937 Size: 969 4594 Acc65I G'GTAC_C Cuts at: 2884 2884 Size: 5563 AclI AA'CG_TT Cuts at: 1563 3690 4063 1563 Size: 2127 373 3063 Fragments arranged by size: 3063 2127 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3626 5010 5278 1632 Size: 1994 1384 268 1917 Fragments arranged by size: 1994 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5366 AflIII A'CryG_T Cuts at: 1203 4462 5439 1203 Size: 3259 977 1327 Fragments arranged by size: 3259 1327 977 AgeI A'CCGG_T Cuts at: 4394 4394 Size: 5563 AhdI GACnn_n'nnGTC Cuts at: 4292 4292 Size: 5563 AleI CACnn'nnGTG Cuts at: 1166 2696 1166 Size: 1530 4033 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5563 AlwNI CAG_nnn'CTG Cuts at: 3306 4878 3306 Size: 1572 3991 ApaLI G'TGCA_C Cuts at: 3620 4776 3620 Size: 1156 4407 ApoI r'AATT_y Cuts at: 1493 2872 3098 3250 5359 1493 Size: 1379 226 152 2109 1697 Fragments arranged by size: 2109 1697 1379 226 152 AseI AT'TA_AT Cuts at: 160 1032 4117 160 Size: 872 3085 1606 Fragments arranged by size: 3085 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5563 AvaI C'yCGr_G Cuts at: 1870 1943 2028 2847 2888 1870 Size: 73 85 819 41 4545 Fragments arranged by size: 4545 819 85 73 41 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2247 2280 2247 Size: 33 5530 BamHI G'GATC_C Cuts at: 2893 3260 2893 Size: 367 5196 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2466 2604 2633 2854 2884 Size: 325 217 627 679 138 29 221 30 Cuts at: 2884 4339 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 679 627 325 221 217 138 30 29 BanII G_rGCy'C Cuts at: 1391 2029 2203 2882 1391 Size: 638 174 679 4072 Fragments arranged by size: 4072 679 638 174 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4592 BbvCI CC'TCA_GC Cuts at: 2181 2181 Size: 5563 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3754 3788 1903 Size: 34 1817 34 3678 Fragments arranged by size: 3678 1817 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2292 3455 4665 2292 Size: 1163 1210 3190 Fragments arranged by size: 3190 1210 1163 BclI T'GATC_A Cuts at: 1548 1548 Size: 5563 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4174 136 Size: 107 122 71 631 605 2502 1525 Fragments arranged by size: 2502 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5558 5558 Size: 5563 BlpI GC'TnA_GC Cuts at: 2076 2980 2076 Size: 904 4659 Bme1580I G_kGCm'C Cuts at: 1790 2609 2784 2857 3624 4780 1790 Size: 819 175 73 767 1156 2573 Fragments arranged by size: 2573 1156 819 767 175 73 BmgBI CAC'GTC Cuts at: 1318 2299 1318 Size: 981 4582 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3294 4252 442 Size: 416 53 83 399 24 1877 958 1753 Fragments arranged by size: 1877 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5563 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4223 1239 Size: 2984 2579 Bpu10I CC'TnA_GC Cuts at: 2181 2205 2181 Size: 24 5539 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3688 4553 4851 3688 Size: 865 298 4400 Fragments arranged by size: 4400 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4226 882 Size: 617 2727 2219 Fragments arranged by size: 2727 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2195 3354 493 Size: 711 991 1159 2702 Fragments arranged by size: 2702 1159 991 711 BsaBI GATnn'nnATC Cuts at: 5557 5557 Size: 5563 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3370 3752 4387 275 Size: 53 83 186 936 1837 382 635 1451 Fragments arranged by size: 1837 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3996 4394 4668 4815 5226 3996 Size: 398 274 147 411 4333 Fragments arranged by size: 4333 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5338 Fragments arranged by size: 5338 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5563 BseYI C'CCAG_C Cuts at: 4766 5262 4766 Size: 496 5067 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4990 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3013 3774 4802 1540 Size: 275 186 1012 761 1028 2301 Fragments arranged by size: 2301 1028 1012 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2203 3624 3709 4780 2203 Size: 1421 85 1071 2986 Fragments arranged by size: 2986 1421 1071 85 BsmI GAATG_Cn' Cuts at: 3074 3167 3074 Size: 93 5470 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2203 2609 2784 2857 2882 3624 Size: 399 239 174 406 175 73 25 742 Cuts at: 3624 3709 4780 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 406 399 239 175 174 85 73 25 BspEI T'CCGG_A Cuts at: 5226 5226 Size: 5563 BspHI T'CATG_A Cuts at: 3452 5182 3452 Size: 1730 3833 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2176 2918 844 Size: 1332 742 3489 Fragments arranged by size: 3489 1332 742 BsrBI CCG'CTC Cuts at: 1394 1823 3450 1394 Size: 429 1627 3507 Fragments arranged by size: 3507 1627 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2070 2557 4058 4232 65 Size: 1183 822 487 1501 174 1396 Fragments arranged by size: 1501 1396 1183 822 487 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2360 4207 4394 1700 Size: 110 550 1847 187 2869 Fragments arranged by size: 2869 1847 550 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5563 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5563 BssSI C'ACGA_G Cuts at: 2211 3623 4635 2211 Size: 1412 1012 3139 Fragments arranged by size: 3139 1412 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2473 2779 794 Size: 1679 306 3578 Fragments arranged by size: 3578 1679 306 BstBI TT'CG_AA Cuts at: 4367 4367 Size: 5563 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5563 BstXI CCAn_nnnn'nTGG Cuts at: 2117 2117 Size: 5563 BstYI r'GATC_y Cuts at: 1972 2893 3260 3645 3662 5114 5200 5212 5558 Size: 921 367 385 17 1452 86 12 346 Cuts at: 5558 1972 Size: 1977 Fragments arranged by size: 1977 1452 921 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4072 Fragments arranged by size: 4072 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4824 Fragments arranged by size: 4824 683 56 BtsI GCAGTG_nn' Cuts at: 2088 3079 3873 3893 2088 Size: 991 794 20 3758 Fragments arranged by size: 3758 991 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5563 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5528 DraI TTT'AAA Cuts at: 2867 2867 Size: 5563 DraIII CAC_nnn'GTG Cuts at: 2473 2473 Size: 5563 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4570 809 Size: 507 3254 1802 Fragments arranged by size: 3254 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2119 2512 3010 Size: 54 1425 50 275 18 289 393 498 Cuts at: 3010 3899 5528 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 498 393 289 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3010 1537 Size: 275 1198 4090 Fragments arranged by size: 4090 1198 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2496 2670 3493 1353 Size: 1143 174 823 3423 Fragments arranged by size: 3423 1143 823 174 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4150 4524 4670 387 Size: 1064 138 2561 374 146 1280 Fragments arranged by size: 2561 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2201 2201 Size: 5563 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3626 4223 5010 5278 1239 Size: 393 1994 597 787 268 1524 Fragments arranged by size: 1994 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2730 2730 Size: 5563 EcoRI G'AATT_C Cuts at: 2872 3250 2872 Size: 378 5185 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2734 2734 Size: 5563 FspI TGC'GCA Cuts at: 2480 4069 2480 Size: 1589 3974 HaeII r_GCGC'y Cuts at: 1678 4710 1678 Size: 3032 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2576 2608 2821 2853 4204 Size: 32 135 32 1004 32 213 32 1351 Cuts at: 4204 4236 4278 4310 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1351 1004 213 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2296 2907 3153 669 Size: 988 281 358 611 246 3079 Fragments arranged by size: 3079 988 611 358 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5563 HpaI GTT'AAC Cuts at: 3153 3153 Size: 5563 KpnI G_GTAC'C Cuts at: 2888 2888 Size: 5563 MfeI C'AATT_G Cuts at: 3162 3162 Size: 5563 MluI A'CGCG_T Cuts at: 5439 5439 Size: 5563 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4677 4861 1305 Size: 3372 184 2007 Fragments arranged by size: 3372 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2121 2514 10 Size: 54 1425 343 289 393 3059 Fragments arranged by size: 3059 1425 393 343 289 54 MslI CAynn'nnrTG Cuts at: 518 1166 2580 2696 3521 3880 4039 518 Size: 648 1414 116 825 359 159 2042 Fragments arranged by size: 2042 1414 825 648 359 159 116 MspA1I CmG'CkG Cuts at: 1606 2979 3656 4804 5049 1606 Size: 1373 677 1148 245 2120 Fragments arranged by size: 2120 1373 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4072 Fragments arranged by size: 4072 939 552 NdeI CA'TA_TG Cuts at: 387 2695 387 Size: 2308 3255 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5563 NotI GC'GGCC_GC Cuts at: 3010 3010 Size: 5563 NspI r_CATG'y Cuts at: 1132 1229 2921 4466 1132 Size: 97 1692 1545 2229 Fragments arranged by size: 2229 1692 1545 97 PasI CC'CwG_GG Cuts at: 1348 2859 1348 Size: 1511 4052 PciI A'CATG_T Cuts at: 4462 4462 Size: 5563 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5563 PmeI GTTT'AAAC Cuts at: 2867 2867 Size: 5563 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2016 2048 2153 2185 3632 3664 5145 5177 2016 Size: 32 105 32 1447 32 1481 32 2402 Fragments arranged by size: 2402 1481 1447 105 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2730 2730 Size: 5563 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5563 PsiI TTA'TAA Cuts at: 3133 3133 Size: 5563 PspXI vC'TCGA_Gb Cuts at: 2847 2847 Size: 5563 PstI C_TGCA'G Cuts at: 830 2915 830 Size: 2085 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5563 SacI G_AGCT'C Cuts at: 2203 2203 Size: 5563 SalI G'TCGA_C Cuts at: 1936 2905 1936 Size: 969 4594 SbfI CC_TGCA'GG Cuts at: 2915 2915 Size: 5563 ScaI AGT'ACT Cuts at: 3811 5523 3811 Size: 1712 3851 SfcI C'TryA_G Cuts at: 826 1045 2911 4046 4727 4918 826 Size: 219 1866 1135 681 191 1471 Fragments arranged by size: 1866 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5563 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5563 SmaI CCC'GGG Cuts at: 1872 2890 1872 Size: 1018 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2847 3667 4568 4830 820 Size: 197 926 904 820 901 262 1553 Fragments arranged by size: 1553 926 904 901 820 262 197 SnaBI TAC'GTA Cuts at: 493 2195 3354 493 Size: 1702 1159 2702 Fragments arranged by size: 2702 1702 1159 SpeI A'CTAG_T Cuts at: 152 152 Size: 5563 SphI G_CATG'C Cuts at: 1132 2921 1132 Size: 1789 3774 SspI AAT'ATT Cuts at: 5 52 3487 5 Size: 47 3435 2081 Fragments arranged by size: 3435 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5563 StyI C'CwwG_G Cuts at: 513 1065 2004 2718 3002 513 Size: 552 939 714 284 3074 Fragments arranged by size: 3074 939 714 552 284 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2269 3590 3607 3760 3945 4390 325 Size: 1255 689 1321 17 153 185 445 1498 Fragments arranged by size: 1498 1321 1255 689 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2688 3809 5521 Size: 274 80 33 51 1443 711 1121 1712 Cuts at: 5521 96 Size: 138 Fragments arranged by size: 1712 1443 1121 711 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5523 5555 5523 Size: 32 5531 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5563 XbaI T'CTAG_A Cuts at: 2798 2899 2798 Size: 101 5462 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2114 2460 2114 Size: 346 5217 XhoI C'TCGA_G Cuts at: 1943 2847 1943 Size: 904 4659 XmaI C'CCGG_G Cuts at: 1870 2888 1870 Size: 1018 4545 XmnI GAAnn'nnTTC Cuts at: 3692 3692 Size: 5563 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3371 276 Size: 53 83 186 936 1837 2468 Fragments arranged by size: 2468 1837 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI