################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HgaI HincII HindII HindIII HinfI HpaI KpnI Ksp632I MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcsI 481 AAATTC [GA]^AATT[CT] AlwI 387 GATCC GGATC(4/5) ApaI 63 GGGCCC GGGCC^C ApoI 481 AAATTC [GA]^AATT[CT] BanII 63 GGGCCC G[GA]GC[CT]^C BbvCI 609 CCTCAGC CCTCAGC(-5/-2) BglI 49 GCCGCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 214 GCTGAGC GC^T[ACGT]AGC Bpu1102I 214 GCTGAGC GC^T[ACGT]AGC BsaAI 289 TACGTA [CT]AC^GT[GA] BsaI 78 GGTCTC GGTCTC(1/5) BsaWI 234 TCCGGT [AT]^CCGG[AT] BsmBI 314 GAGACG CGTCTC(1/5) BsrBI 217 GAGCGG CCGCTC(-3/-3) BsrFI 39 GCCGGC [GA]^CCGG[CT] BsrI 403 ACTGG ACTGG(1/-1) BstNI 154 CCTGG CC^[AT]GG CelII 214 GCTGAGC GC^T[ACGT]AGC Cfr10I 39 GCCGGC [GA]^CCGG[CT] DpnII 387 GATC ^GATC EaeI 305 CGGCCA [CT]^GGCC[GA] EcoRII 154 CCTGG ^CC[AT]GG HphI 650 TCACC GGTGA(8/7) MboI 387 GATC ^GATC MslI 624 CATCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 154 CCTGG CC^[AT]GG NaeI 39 GCCGGC GCC^GGC NcoI 587 CCATGG C^CATGG NdeII 387 GATC ^GATC NgoMIV 39 GCCGGC G^CCGGC NlaIII 588 CATG CATG^ PstI 6 CTGCAG CTGCA^G Sau3AI 387 GATC ^GATC SmaI 60 CCCGGG CCC^GGG SnaBI 289 TACGTA TAC^GTA StuI 434 AGGCCT AGG^CCT Tsp45I 400 GTGAC ^GT[GC]AC XcmI 335 CCAGCACGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 60 CCCGGG C^CCGGG XmaI 60 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 36 GGCGCC G[GA]^CG[CT]C 255 GGCGCC AfaI 292 GTAC GT^AC 576 GTAC AlwNI 167 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 398 CAGTGACTG AvaI 28 CCCGAG C^[CT]CG[GA]G 60 CCCGGG BanI 36 GGCGCC G^G[CT][GA]CC 255 GGCGCC BbeI 36 GGCGCC GGCGC^C 255 GGCGCC BpmI 102 CTCCAG CTGGAG(16/14) 161 CTGGAG BsaHI 36 GGCGCC G[GA]^CG[CT]C 255 GGCGCC BsmAI 314 GAGAC GTCTC(1/5) 79 GTCTC BsoBI 28 CCCGAG C^[CT]CG[GA]G 60 CCCGGG EcoT14I 188 CCTTGG C^C[AT][AT]GG 587 CCATGG HaeII 36 GGCGCC [GA]GCGC^[CT] 255 GGCGCC Hin1I 36 GGCGCC G[GA]^CG[CT]C 255 GGCGCC KasI 36 GGCGCC G^GCGCC 255 GGCGCC KspI 113 CCGCGG CCGC^GG 134 CCGCGG MaeII 290 ACGT A^CGT 317 ACGT MaeIII 400 GTGAC ^GT[ACGT]AC 407 GTTAC MboII 370 GAAGA GAAGA(8/7) 633 GAAGA NarI 36 GGCGCC GG^CGCC 255 GGCGCC PpuMI 150 GGGACCT [GA]G^G[AT]CC[CT] 183 GGGACCC RsaI 292 GTAC GT^AC 576 GTAC SacII 113 CCGCGG CCGC^GG 134 CCGCGG SfcI 6 CTGCAG C^T[GA][CT]AG 511 CTACAG SfoI 36 GGCGCC GGC^GCC 255 GGCGCC StyI 188 CCTTGG C^C[AT][AT]GG 587 CCATGG Tsp509I 453 AATT ^AATT 482 AATT TspRI 398 CAGTG CA[GC]TG[ACGT][ACGT]^ 517 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AccII AciI AluI AvaII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstUI Cac8I CfoI Cfr13I DdeI DraII DsaI EcoO109I FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspA1I MspI MvnI MwoI NciI NlaIV PvuII Sau96I ScrFI SfaNI TseI