Restriction map for
pFN21AB8948
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvrII BalI BamHI
BbrPI BbsI BbvCI BciVI BclI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaI BseAI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI HincII HindII
HindIII HpaI HphI KpnI MaeIII
MamI MfeI MflI MluNI MroI
MscI MseI MslI MunI NcoI
NdeI NheI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI SexAI SfaNI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XcmI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AatII 743 GACGTC GACGT^C
AfaI 67 GTAC GT^AC
AflIII 8 ACGCGT A^C[GA][CT]GT
ApaI 216 GGGCCC GGGCC^C
AviII 74 TGCGCA TGC^GCA
BcgI 92 GCAGCCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 142 GCCCGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 123 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 123 GCTGAGC GC^T[ACGT]AGC
BsmAI 277 GAGAC GTCTC(1/5)
BsmBI 277 GAGACG CGTCTC(1/5)
BspMI 285 GCAGGT ACCTGC(4/8)
BssSI 115 CACGAG CACGAG(-5/-1)
BstAPI 198 GCAGGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 26 CATCC GGATG(2/0)
CelII 123 GCTGAGC GC^T[ACGT]AGC
EarI 97 CTCTTC CTCTTC(1/4)
EcoNI 384 CCTCGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 26 CATCC GGATG(9/13)
FspI 74 TGCGCA TGC^GCA
Ksp632I 97 CTCTTC CTCTTC(1/4)
MaeII 744 ACGT A^CGT
MluI 8 ACGCGT A^CGCGT
NlaIII 204 CATG CATG^
NotI 763 GCGGCCGC GC^GGCCGC
NspI 203 ACATGC [GA]CATG^[CT]
PpuMI 55 GGGACCT [GA]G^G[AT]CC[CT]
RsaI 67 GTAC GT^AC
ScaI 66 AGTACT AGT^ACT
SfiI 141 GGCCCGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 69 ACTAGT A^CTAGT
TspRI 232 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
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AlwNI 81 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
845 CAGCGCCTG
BanII 216 GGGCCC G[GA]GC[CT]^C
912 GGGCTC
BfaI 70 CTAG C^TAG
410 CTAG
BpmI 464 CTCCAG CTGGAG(16/14)
578 CTCCAG
BseRI 118 GAGGAG GAGGAG(10/8)
388 GAGGAG
BsgI 724 CTGCAC GTGCAG(16/14)
283 GTGCAG
HinfI 253 GACTC G^A[ACGT]TC
370 GAGTC
MaeI 70 CTAG C^TAG
410 CTAG
MboII 98 TCTTC GAAGA(8/7)
824 TCTTC
NaeI 360 GCCGGC GCC^GGC
754 GCCGGC
NgoMIV 360 GCCGGC G^CCGGC
754 GCCGGC
PaeR7I 355 CTCGAG C^TCGAG
385 CTCGAG
PleI 253 GACTC GAGTC(4/5)
370 GAGTC
PstI 136 CTGCAG CTGCA^G
229 CTGCAG
PvuII 403 CAGCTG CAG^CTG
721 CAGCTG
SfcI 136 CTGCAG C^T[GA][CT]AG
229 CTGCAG
XhoI 355 CTCGAG C^TCGAG
385 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
AvaII BanI BbeI BbvI BcnI
BmyI BsaHI BsaJI BsaWI BsiEI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
KasI KspI MboI MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIV SacII Sau3AI
Sau96I ScrFI SfoI SmlI TaqI
TseI TthHB8I