(Circular) (Six-base) MAPSORT of: pFN21AB8948.seq Check: 7762 from: 1 to: 5653 pFN21AB8948 5653 bp With 182 enzymes: SgfI * August 14, 2012 18:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2281 2281 Size: 5653 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2752 3463 278 Size: 53 83 186 936 1216 711 2468 Fragments arranged by size: 2468 1216 936 711 186 83 53 AccI GT'mk_AC Cuts at: 1937 2996 1937 Size: 1059 4594 Acc65I G'GTAC_C Cuts at: 2974 2974 Size: 5653 AclI AA'CG_TT Cuts at: 1563 3780 4153 1563 Size: 2217 373 3063 Fragments arranged by size: 3063 2217 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3716 5100 5368 1632 Size: 2084 1384 268 1917 Fragments arranged by size: 2084 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5456 AflIII A'CryG_T Cuts at: 1203 2013 4552 5529 1203 Size: 810 2539 977 1327 Fragments arranged by size: 2539 1327 977 810 AgeI A'CCGG_T Cuts at: 4484 4484 Size: 5653 AhdI GACnn_n'nnGTC Cuts at: 4382 4382 Size: 5653 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5653 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2963 1184 Size: 1779 3874 AlwNI CAG_nnn'CTG Cuts at: 2091 2855 3396 4968 2091 Size: 764 541 1572 2776 Fragments arranged by size: 2776 1572 764 541 ApaI G_GGCC'C Cuts at: 2225 2225 Size: 5653 ApaLI G'TGCA_C Cuts at: 3710 4866 3710 Size: 1156 4497 ApoI r'AATT_y Cuts at: 1493 2962 3188 3340 5449 1493 Size: 1469 226 152 2109 1697 Fragments arranged by size: 2109 1697 1469 226 152 AseI AT'TA_AT Cuts at: 160 1032 4207 160 Size: 872 3175 1606 Fragments arranged by size: 3175 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 AvaI C'yCGr_G Cuts at: 1870 1943 2360 2390 2734 2978 1870 Size: 73 417 30 344 244 4545 Fragments arranged by size: 4545 417 344 244 73 30 BamHI G'GATC_C Cuts at: 2983 3350 2983 Size: 367 5286 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2263 2317 2398 2659 2762 Size: 325 217 627 476 54 81 261 103 Cuts at: 2762 2974 4429 618 Size: 212 1455 1842 Fragments arranged by size: 1842 1455 627 476 325 261 217 212 103 81 54 BanII G_rGCy'C Cuts at: 1391 2225 2921 2972 1391 Size: 834 696 51 4072 Fragments arranged by size: 4072 834 696 51 BbeI G_GCGC'C Cuts at: 2321 2402 2663 2321 Size: 81 261 5311 Fragments arranged by size: 5311 261 81 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4682 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2086 2120 3844 3878 1903 Size: 34 149 34 1724 34 3678 Fragments arranged by size: 3678 1724 149 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3545 4755 3545 Size: 1210 4443 BclI T'GATC_A Cuts at: 1548 1548 Size: 5653 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2153 4264 136 Size: 107 122 71 631 605 481 2111 1525 Fragments arranged by size: 2111 1525 631 605 481 122 107 71 BglII A'GATC_T Cuts at: 5648 5648 Size: 5653 BlpI GC'TnA_GC Cuts at: 2129 3070 2129 Size: 941 4712 Bme1580I G_kGCm'C Cuts at: 1790 2225 2268 3714 4870 1790 Size: 435 43 1446 1156 2573 Fragments arranged by size: 2573 1446 1156 435 43 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5653 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2063 2447 3384 Size: 416 53 83 399 24 646 384 937 Cuts at: 3384 4342 442 Size: 958 1753 Fragments arranged by size: 1753 958 937 646 416 399 384 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2454 2568 4313 1239 Size: 1215 114 1745 2579 Fragments arranged by size: 2579 1745 1215 114 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2649 3778 4643 4941 2649 Size: 1129 865 298 3361 Fragments arranged by size: 3361 1129 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4316 882 Size: 617 2817 2219 Fragments arranged by size: 2817 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3444 493 Size: 711 2240 2702 Fragments arranged by size: 2702 2240 711 BsaBI GATnn'nnATC Cuts at: 5647 5647 Size: 5653 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2230 2318 2399 2438 Size: 53 83 186 936 697 88 81 39 Cuts at: 2438 2660 2749 3460 3842 4477 275 Size: 222 89 711 382 635 1451 Fragments arranged by size: 1451 936 711 697 635 382 222 186 89 88 83 81 53 39 BsaWI w'CCGG_w Cuts at: 2494 2506 2518 4086 4484 4758 4905 5316 2494 Size: 12 12 1568 398 274 147 411 2831 Fragments arranged by size: 2831 1568 411 398 274 147 12 12 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5428 Fragments arranged by size: 5428 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2138 2408 1186 Size: 952 270 4431 Fragments arranged by size: 4431 952 270 BseYI C'CCAG_C Cuts at: 2184 2602 4856 5352 2184 Size: 418 2254 496 2485 Fragments arranged by size: 2485 2254 496 418 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2309 2714 1163 Size: 573 573 405 4102 Fragments arranged by size: 4102 573 573 405 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2337 2370 2499 2511 2523 2529 Size: 275 186 336 33 129 12 12 6 Cuts at: 2529 2760 2772 3103 3864 4892 1540 Size: 231 12 331 761 1028 2301 Fragments arranged by size: 2301 1028 761 336 331 275 231 186 129 33 12 12 12 6 BsiHKAI G_wGCw'C Cuts at: 3714 3799 4870 3714 Size: 85 1071 4497 Fragments arranged by size: 4497 1071 85 BsmI GAATG_Cn' Cuts at: 3164 3257 3164 Size: 93 5560 BsmBI CGTCTCn'nnnn_ Cuts at: 2276 2276 Size: 5653 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2225 2268 2921 2972 3714 3799 4870 Size: 399 435 43 653 51 742 85 1071 Cuts at: 4870 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 653 435 399 85 51 43 BspEI T'CCGG_A Cuts at: 5316 5316 Size: 5653 BspHI T'CATG_A Cuts at: 3542 5272 3542 Size: 1730 3923 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2281 3008 844 Size: 1437 727 3489 Fragments arranged by size: 3489 1437 727 BsrBI CCG'CTC Cuts at: 1394 1823 3540 1394 Size: 429 1717 3507 Fragments arranged by size: 3507 1717 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4148 4322 65 Size: 1183 2900 174 1396 Fragments arranged by size: 2900 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2110 2365 2759 4297 4484 1700 Size: 110 300 255 394 1538 187 2869 Fragments arranged by size: 2869 1538 394 300 255 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5653 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5653 BssSI C'ACGA_G Cuts at: 2120 3713 4725 2120 Size: 1593 1012 3048 Fragments arranged by size: 3048 1593 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2209 794 Size: 1415 4238 BstBI TT'CG_AA Cuts at: 4457 4457 Size: 5653 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5653 BstYI r'GATC_y Cuts at: 1972 2983 3350 3735 3752 5204 5290 5302 5648 Size: 1011 367 385 17 1452 86 12 346 Cuts at: 5648 1972 Size: 1977 Fragments arranged by size: 1977 1452 1011 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2056 2321 2754 2766 513 Size: 552 991 265 433 12 3400 Fragments arranged by size: 3400 991 552 433 265 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2886 487 Size: 683 56 1660 3254 Fragments arranged by size: 3254 1660 683 56 BtsI GCAGTG_nn' Cuts at: 2243 3169 3963 3983 2243 Size: 926 794 20 3913 Fragments arranged by size: 3913 926 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5653 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5618 DraI TTT'AAA Cuts at: 2957 2957 Size: 5653 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4660 809 Size: 507 3344 1802 Fragments arranged by size: 3344 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2154 2367 2704 Size: 54 1425 50 275 18 324 213 337 Cuts at: 2704 2757 2769 3100 3989 5618 8 Size: 53 12 331 889 1629 43 Fragments arranged by size: 1629 1425 889 337 331 324 275 213 54 53 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2367 2757 2769 3100 1537 Size: 275 555 390 12 331 4090 Fragments arranged by size: 4090 555 390 331 275 12 EarI CTCTTCn'nnn_ Cuts at: 1353 2108 3583 1353 Size: 755 1475 3423 Fragments arranged by size: 3423 1475 755 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4240 4614 4760 387 Size: 1064 138 2651 374 146 1280 Fragments arranged by size: 2651 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2454 2568 3716 4313 5100 5368 1239 Size: 393 822 114 1148 597 787 268 1524 Fragments arranged by size: 1524 1148 822 787 597 393 268 114 EcoNI CCTnn'n_nnAGG Cuts at: 2393 2393 Size: 5653 EcoO109I rG'GnC_Cy Cuts at: 2061 2221 2222 2061 Size: 160 1 5492 Fragments arranged by size: 5492 160 1 EcoRI G'AATT_C Cuts at: 2962 3340 2962 Size: 378 5275 FspI TGC'GCA Cuts at: 2081 4159 2081 Size: 2078 3575 FspAI rTGC'GCAy Cuts at: 2081 2081 Size: 5653 HaeII r_GCGC'y Cuts at: 1678 2091 2302 2321 2402 2663 2676 2781 2855 Size: 413 211 19 81 261 13 105 74 Cuts at: 2855 2904 4800 1678 Size: 49 1896 2531 Fragments arranged by size: 2531 1896 413 261 211 105 81 74 49 19 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2029 2061 2243 2275 2849 Size: 32 135 32 457 32 182 32 574 Cuts at: 2849 2881 4294 4326 4368 4400 1373 Size: 32 1413 32 42 32 2626 Fragments arranged by size: 2626 1413 574 457 182 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2997 3243 669 Size: 988 281 1059 246 3079 Fragments arranged by size: 3079 1059 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5653 HpaI GTT'AAC Cuts at: 3243 3243 Size: 5653 KasI G'GCGC_C Cuts at: 2317 2398 2659 2317 Size: 81 261 5311 Fragments arranged by size: 5311 261 81 KpnI G_GTAC'C Cuts at: 2978 2978 Size: 5653 MfeI C'AATT_G Cuts at: 3252 3252 Size: 5653 MluI A'CGCG_T Cuts at: 2013 5529 2013 Size: 3516 2137 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2034 4767 4951 1305 Size: 729 2733 184 2007 Fragments arranged by size: 2733 2007 729 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3831 Fragments arranged by size: 3831 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3611 3970 4129 518 Size: 648 2445 359 159 2042 Fragments arranged by size: 2445 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2184 2215 2323 2372 2410 2728 2756 2768 Size: 578 31 108 49 38 318 28 12 Cuts at: 2768 3069 3746 4894 5139 1606 Size: 301 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 578 318 301 245 108 49 38 31 28 12 NaeI GCC'GGC Cuts at: 2367 2761 2367 Size: 394 5259 NarI GG'CG_CC Cuts at: 2318 2399 2660 2318 Size: 81 261 5311 Fragments arranged by size: 5311 261 81 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5101 NdeI CA'TA_TG Cuts at: 387 387 Size: 5653 NgoMIV G'CCGG_C Cuts at: 2365 2759 2365 Size: 394 5259 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5653 NotI GC'GGCC_GC Cuts at: 2769 3100 2769 Size: 331 5322 NspI r_CATG'y Cuts at: 1132 1229 2212 3011 4556 1132 Size: 97 983 799 1545 2229 Fragments arranged by size: 2229 1545 983 799 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5653 PciI A'CATG_T Cuts at: 4552 4552 Size: 5653 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5653 PmeI GTTT'AAAC Cuts at: 2957 2957 Size: 5653 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3722 3754 5235 5267 3722 Size: 32 1481 32 4108 Fragments arranged by size: 4108 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2061 2061 Size: 5653 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5653 PsiI TTA'TAA Cuts at: 3223 3223 Size: 5653 PspOMI G'GGCC_C Cuts at: 2221 2221 Size: 5653 PspXI vC'TCGA_Gb Cuts at: 2360 2390 2360 Size: 30 5623 PstI C_TGCA'G Cuts at: 830 2145 2238 3005 830 Size: 1315 93 767 3478 Fragments arranged by size: 3478 1315 767 93 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5653 PvuII CAG'CTG Cuts at: 2410 2728 2410 Size: 318 5335 SacII CC_GC'GG Cuts at: 2324 2757 2769 2324 Size: 433 12 5208 Fragments arranged by size: 5208 433 12 SalI G'TCGA_C Cuts at: 1936 2995 1936 Size: 1059 4594 SbfI CC_TGCA'GG Cuts at: 3005 3005 Size: 5653 ScaI AGT'ACT Cuts at: 2073 3901 5613 2073 Size: 1828 1712 2113 Fragments arranged by size: 2113 1828 1712 SfcI C'TryA_G Cuts at: 826 1045 2141 2234 3001 4136 4817 5008 826 Size: 219 1096 93 767 1135 681 191 1471 Fragments arranged by size: 1471 1135 1096 767 681 219 191 93 SfiI GGCCn_nnn'nGGCC Cuts at: 2153 2153 Size: 5653 SfoI GGC'GCC Cuts at: 2319 2400 2661 2319 Size: 81 261 5311 Fragments arranged by size: 5311 261 81 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5653 SmaI CCC'GGG Cuts at: 1872 2980 1872 Size: 1108 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2360 2390 2664 3757 4658 4920 Size: 197 926 417 30 274 1093 901 262 Cuts at: 4920 820 Size: 1553 Fragments arranged by size: 1553 1093 926 901 417 274 262 197 30 SnaBI TAC'GTA Cuts at: 493 3444 493 Size: 2951 2702 SpeI A'CTAG_T Cuts at: 152 2074 152 Size: 1922 3731 SphI G_CATG'C Cuts at: 1132 3011 1132 Size: 1879 3774 SspI AAT'ATT Cuts at: 5 52 3577 5 Size: 47 3525 2081 Fragments arranged by size: 3525 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5653 StyI C'CwwG_G Cuts at: 513 1065 3092 513 Size: 552 2027 3074 Fragments arranged by size: 3074 2027 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2258 3680 3697 3850 4035 4480 325 Size: 1255 678 1422 17 153 185 445 1498 Fragments arranged by size: 1498 1422 1255 678 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2071 3899 5611 Size: 274 80 33 51 1443 94 1828 1712 Cuts at: 5611 96 Size: 138 Fragments arranged by size: 1828 1712 1443 274 138 94 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5613 5645 5613 Size: 32 5621 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5653 XbaI T'CTAG_A Cuts at: 2989 2989 Size: 5653 XhoI C'TCGA_G Cuts at: 1943 2360 2390 1943 Size: 417 30 5206 Fragments arranged by size: 5206 417 30 XmaI C'CCGG_G Cuts at: 1870 2978 1870 Size: 1108 4545 XmnI GAAnn'nnTTC Cuts at: 3782 3782 Size: 5653 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2750 3461 276 Size: 53 83 186 936 1216 711 2468 Fragments arranged by size: 2468 1216 936 711 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI NruI NsiI PacI PfoI PmlI PsrI RsrII SacI SanDI SapI SexAI SrfI SwaI XcmI