################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AflIII AgeI AhdI AocI ApaI AscI AseI AsnI Asp718I AspEI AspI AvrII BalI BanI BbeI BbrPI BcgI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BseRI BsiEI BsiWI BsmBI BsmI BspDI BspLU11I Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SwaI Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 398 TCCGGA T^CCGGA AcsI 1204 GAATTT [GA]^AATT[CT] Alw44I 76 GTGCAC G^TGCAC AlwNI 81 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 76 GTGCAC G^TGCAC ApoI 1204 GAATTT [GA]^AATT[CT] Asp700I 129 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 321 TGCGCA TGC^GCA BamHI 437 GGATCC G^GATCC BbsI 477 GAAGAC GAAGAC(2/6) BbvCI 1410 CCTCAGC CCTCAGC(-5/-2) BpuAI 477 GAAGAC GAAGAC(2/6) BsaAI 211 TACGTG [CT]AC^GT[GA] BsaI 904 GAGACC GGTCTC(1/5) BsaWI 398 TCCGGA [AT]^CCGG[AT] BseAI 398 TCCGGA T^CCGGA BsgI 836 GTGCAG GTGCAG(16/14) Bsp1407I 848 TGTACA T^GTACA BspEI 398 TCCGGA T^CCGGA BspHI 944 TCATGA T^CATGA BsrBI 1473 CCGCTC CCGCTC(-3/-3) BsrDI 425 GCAATG GCAATG(2/0) BsrFI 195 GCCGGT [GA]^CCGG[CT] BsrGI 848 TGTACA T^GTACA BsrI 138 CCAGT ACTGG(1/-1) BssHII 521 GCGCGC G^CGCGC BstAPI 1032 GCAAGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 437 GGATCC [GA]^GATC[CT] Cfr10I 195 GCCGGT [GA]^CCGG[CT] DsaI 20 CCGTGG C^C[GA][CT]GG EaeI 878 TGGCCG [CT]^GGCC[GA] EcoRV 1402 GATATC GAT^ATC FspI 321 TGCGCA TGC^GCA HincII 1390 GTTGAC GT[CT]^[GA]AC HindII 1390 GTTGAC GT[CT]^[GA]AC MflI 437 GGATCC [GA]^GATC[CT] MroI 398 TCCGGA T^CCGGA PpuMI 788 GGGACCT [GA]G^G[AT]CC[CT] PvuII 84 CAGCTG CAG^CTG RcaI 944 TCATGA T^CATGA SmaI 785 CCCGGG CCC^GGG SspBI 848 TGTACA T^GTACA SspI 154 AATATT AAT^ATT StuI 1320 AGGCCT AGG^CCT XhoII 437 GGATCC [GA]^GATC[CT] XmaCI 785 CCCGGG C^CCGGG XmaI 785 CCCGGG C^CCGGG XmnI 129 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccII 312 CGCG CG^CG 522 CGCG AspHI 76 GTGCAC G[AT]GC[AT]^C 754 GAGCAC BanII 216 GGGCTC G[GA]GC[CT]^C 931 GGGCTC BciVI 1138 GGATAC GTATCC(6/5) 1439 GTATCC BpmI 643 CTCCAG CTGGAG(16/14) 1313 CTGGAG BsiHKAI 76 GTGCAC G[AT]GC[AT]^C 754 GAGCAC BsmAI 904 GAGAC GTCTC(1/5) 1121 GAGAC BssSI 808 CTCGTG CACGAG(-5/-1) 1087 CTCGTG BstUI 312 CGCG CG^CG 522 CGCG DraII 102 AGGCCCT [GA]G^G[ACGT]CC[CT] 788 GGGACCT EcoO109I 102 AGGCCCT [GA]G^G[ACGT]CC[CT] 788 GGGACCT EcoT14I 483 CCAAGG C^C[AT][AT]GG 1151 CCAAGG MslI 228 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1083 CACCCTCGTG MspA1I 84 CAGCTG C[AC]G^C[GT]G 1072 CAGCGG MvnI 312 CGCG CG^CG 522 CGCG PleI 1180 GACTC GAGTC(4/5) 14 GAGTC StyI 483 CCAAGG C^C[AT][AT]GG 1151 CCAAGG TspRI 834 CAGTG CA[GC]TG[ACGT][ACGT]^ 1269 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AfaI AluI AlwI Aor51HI AvaI AvaII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EarI Eco47III EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TthHB8I