(Linear) (Six-base) MAPSORT of: KIEE5659.seq Check: 8943 from: 1 to: 1479 KIEE5659.orf With 182 enzymes: SgfI * December 5, 2014 13:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 63 1479 Size: 63 1416 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 740 1479 Size: 740 739 AfeI AGC'GCT Cuts at: 0 187 266 844 1479 Size: 187 79 578 635 Fragments arranged by size: 635 578 187 79 AleI CACnn'nnGTG Cuts at: 0 1087 1479 Size: 1087 392 AlwNI CAG_nnn'CTG Cuts at: 0 86 1479 Size: 86 1393 ApaLI G'TGCA_C Cuts at: 0 76 1479 Size: 76 1403 ApoI r'AATT_y Cuts at: 0 1204 1479 Size: 1204 275 AvaI C'yCGr_G Cuts at: 0 291 785 1118 1479 Size: 291 494 333 361 Fragments arranged by size: 494 361 333 291 BamHI G'GATC_C Cuts at: 0 437 1479 Size: 437 1042 BanII G_rGCy'C Cuts at: 0 220 935 1479 Size: 220 715 544 Fragments arranged by size: 715 544 220 BbsI GAAGACnn'nnnn_ Cuts at: 0 484 1479 Size: 484 995 BbvCI CC'TCA_GC Cuts at: 0 1411 1479 Size: 1411 68 BciVI GTATCCnnnnn_n' Cuts at: 0 1132 1450 1479 Size: 1132 318 29 Fragments arranged by size: 1132 318 29 Bme1580I G_kGCm'C Cuts at: 0 80 1094 1159 1479 Size: 80 1014 65 320 Fragments arranged by size: 1014 320 80 65 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 322 1159 1479 Size: 322 837 320 Fragments arranged by size: 837 322 320 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 628 1334 1479 Size: 628 706 145 Fragments arranged by size: 706 628 145 Bpu10I CC'TnA_GC Cuts at: 0 1411 1479 Size: 1411 68 BsaI GGTCTCn'nnnn_ Cuts at: 0 898 1479 Size: 898 581 BsaAI yAC'GTr Cuts at: 0 213 1479 Size: 213 1266 BsaWI w'CCGG_w Cuts at: 0 398 1479 Size: 398 1081 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 5 35 1479 Size: 5 30 1444 Fragments arranged by size: 1444 30 5 BseYI C'CCAG_C Cuts at: 0 182 664 1479 Size: 182 482 815 Fragments arranged by size: 815 482 182 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 857 1479 Size: 857 622 BsiHKAI G_wGCw'C Cuts at: 0 80 758 1479 Size: 80 678 721 Fragments arranged by size: 721 678 80 Bsp1286I G_dGCh'C Cuts at: 0 80 220 758 935 1094 1159 1479 Size: 80 140 538 177 159 65 320 Fragments arranged by size: 538 320 177 159 140 80 65 BspEI T'CCGG_A Cuts at: 0 398 1479 Size: 398 1081 BspHI T'CATG_A Cuts at: 0 944 1479 Size: 944 535 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 63 366 1109 1479 Size: 63 303 743 370 Fragments arranged by size: 743 370 303 63 BsrBI CCG'CTC Cuts at: 0 1475 1479 Size: 1475 4 BsrDI GCAATG_nn' Cuts at: 0 432 1479 Size: 432 1047 BsrFI r'CCGG_y Cuts at: 0 195 1479 Size: 195 1284 BsrGI T'GTAC_A Cuts at: 0 848 1479 Size: 848 631 BssHII G'CGCG_C Cuts at: 0 521 1479 Size: 521 958 BssSI C'ACGA_G Cuts at: 0 808 1087 1479 Size: 808 279 392 Fragments arranged by size: 808 392 279 BstAPI GCAn_nnn'nTGC Cuts at: 0 1038 1479 Size: 1038 441 BstYI r'GATC_y Cuts at: 0 437 1479 Size: 437 1042 BtgI C'CryG_G Cuts at: 0 20 1479 Size: 20 1459 EaeI y'GGCC_r Cuts at: 0 878 1479 Size: 878 601 EarI CTCTTCn'nnn_ Cuts at: 0 139 556 959 1479 Size: 139 417 403 520 Fragments arranged by size: 520 417 403 139 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 628 740 1334 1479 Size: 628 112 594 145 Fragments arranged by size: 628 594 145 112 EcoO109I rG'GnC_Cy Cuts at: 0 103 789 1479 Size: 103 686 690 Fragments arranged by size: 690 686 103 EcoRV GAT'ATC Cuts at: 0 1404 1479 Size: 1404 75 FspI TGC'GCA Cuts at: 0 323 1479 Size: 323 1156 FspAI rTGC'GCAy Cuts at: 0 323 1479 Size: 323 1156 HaeII r_GCGC'y Cuts at: 0 189 268 846 1479 Size: 189 79 578 633 Fragments arranged by size: 633 578 189 79 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 274 306 721 753 917 949 1130 1162 Size: 274 32 415 32 164 32 181 32 Cuts at: 1162 1479 Size: 317 Fragments arranged by size: 415 317 274 181 164 32 32 32 32 HincII GTy'rAC Cuts at: 0 1392 1479 Size: 1392 87 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 122 1121 1479 Size: 122 999 358 Fragments arranged by size: 999 358 122 MslI CAynn'nnrTG Cuts at: 0 232 1087 1479 Size: 232 855 392 Fragments arranged by size: 855 392 232 MspA1I CmG'CkG Cuts at: 0 86 1074 1479 Size: 86 988 405 Fragments arranged by size: 988 405 86 PpuMI rG'GwC_Cy Cuts at: 0 789 1479 Size: 789 690 PvuII CAG'CTG Cuts at: 0 86 1479 Size: 86 1393 SmaI CCC'GGG Cuts at: 0 787 1479 Size: 787 692 SspI AAT'ATT Cuts at: 0 156 1479 Size: 156 1323 StuI AGG'CCT Cuts at: 0 1322 1479 Size: 1322 157 StyI C'CwwG_G Cuts at: 0 483 1151 1479 Size: 483 668 328 Fragments arranged by size: 668 483 328 TatI w'GTAC_w Cuts at: 0 848 1265 1479 Size: 848 417 214 Fragments arranged by size: 848 417 214 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 5 37 1289 1321 1479 Size: 5 32 1252 32 158 Fragments arranged by size: 1252 158 32 32 5 XmaI C'CCGG_G Cuts at: 0 785 1479 Size: 785 694 XmnI GAAnn'nnTTC Cuts at: 0 133 1479 Size: 133 1346 Enzymes that do cut: AarI AcuI AfeI AleI AlwNI ApaLI ApoI AvaI BamHI BanII BbsI BbvCI BciVI Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaWI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstYI BtgI EaeI EarI Eco57MI EcoO109I EcoRV FspI FspAI HaeII Hin4I HincII MmeI MslI MspA1I PpuMI PvuII SmaI SspI StuI StyI TatI TstI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI BanI BbeI BcgI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BpuEI BsaBI BsaHI BseRI BsiEI BsiWI BsmI BsmBI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI FalI FseI HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SwaI TaqII Tth111I XbaI XcmI XhoI ZraI